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Stabilitas Ekstrak Bunga Telang (Clitoria ternatea L.) Terenkapsulasi Maltodekstrin dan Gelatin dan Potensi Prebiotiknya Sari, Devina Puspita; Aditiyarini, Dwi; Ariestanti, Catarina Aprilia
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 9, No 3 (2024): October 2024
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v9i3.7550

Bunga telang merupakan salah satu bunga yang dapat dimakan dan digunakan sebagai pewarna alami makanan. Bunga ini juga berpotensi sebagai antioksidan dan prebiotik akibat kandungan antosianinnya. Namun ekstrak bunga telang mudah terdegradasi. Stabilitas zat warna bunga telang dapat dipertahankan dengan teknik enkapsulasi menggunakan maltodekstrin dan gelatin. Penelitian ini dilakukan untuk mengetahui rasio maltodekstrin dan gelatin yang menghasilkan stabilitas enkapsulat bunga telang terbaik selama 15 hari penyimpanan dan potensi prebiotiknya. Ekstrak bunga telang diperoleh melalui metode maserasi dengan pelarut etanol 70%. Rasio maltodekstrin dan gelatin yang digunakan adalah 1:1, 3:1 dan 5:1, dengan kadar ekstrak bunga telang 1%. Stabilitas enkapsulat dianalisis dari kandungan antosianin dan antioksidan dengan waktu uji setiap 5 hari selama 15 hari. Kadar antosianin diukur dengan metode pH differential. Aktivitas antioksidan diukur dengan metode DPPH. Potensi prebiotik diuji menggunakan Lactobacillus bulgaricus. Hasil menunjukkan bahwa enkapsulat ekstrak bunga telang dengan rasio maltodekstrin dan gelatin sebesar 3:1 memiliki stabilitas enkapsulat terbaik dengan kadar air 6,863%, kadar antosianin 0,217 mg/g, dan daya redam radikal bebas sebesar 54,858%. Enkapsulat ini juga terbukti berpotensi menjadi prebiotik yang ditandai dengan pertumbuhan L.bulgaricus sebesar 2,85 x 109 CFU/mL.

Antibacterial Activity of Dendrophthoe pentandra (L.) Leaves Extract Against Staphylococcus aureus and Escherichia coli Inggrid, Annabelle Marie; Aditiyarini, Dwi; Prakasita, Vinsa Cantya
Jurnal Biodjati Vol 9, No 2 (2024): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v9i2.30436

Sapuran, Wonosobo is a popular area with several local tea plantations, which are infested by mistletoe, such as Dendrophthoe pentandra (L.) Miq. Mistletoe has been widely reported to have antibacterial activity due to its secondary metabolite content. Therefore, this study aimed to identify and explore the antibacterial activity of D. pentandra leaves extract against Staphylococcus aureus and Escherichia coli. The test samples were extracted using the maceration method, followed by phytochemical screening and total assay of flavonoids, phenols, and tannins. Antibacterial activity tests were carried out using disk diffusion, Minimum Inhibitory Concentration (MIC), and Minimum Bactericidal Concentration (MBC). The yield from the maceration method using 96% ethanol solvent was 8.78%, containing secondary metabolite compounds, including alkaloids, saponins, steroids, terpenoids, and flavonoids. The total flavonoid, phenol, and tanin content obtained were 4.09 ± 0.70 mg QE/g, 1.76 ± 0.16 g GAE/g, and 1.37 ± 0.14 mg TAE/g, respectively. In addition, the extract showed medium inhibition of S. aureus at a concentration of 400 and 525 mg/m, with no inhibitory effect against E. coli. The MIC and MBC from the microdilution method for S. aureus were at 4400 and 8800 mg/mL extract, while values of 28000 and 56000 mg/mL were obtained for E. coli. Based on these results, the inhibition activity of ethanol extract of D. pentandra against S. aureus was greater compared to E. coli.

Stability of encapsulated red dragon fruit (Hylocereus polyrhizus) peel: effects on antioxidant and prebiotic potency Justina, Fayola; Aditiyarini, Dwi; Ariestanti, Catarina Aprilia
Advances in Food Science, Sustainable Agriculture and Agroindustrial Engineering (AFSSAAE) Vol 7, No 4 (2024)
Publisher : Advances in Food Science, Sustainable Agriculture and Agroindustrial Engineering (AFSSAAE)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.afssaae.2024.007.04.1

The high consumption of red dragon fruit has led to an increase in red dragon fruit peel waste in the environment, which has potential as a source of natural food coloring and as a prebiotic but is susceptible todegraded. The objective of this study was to determine the best ratio of maltodextrin and gelatin as encapsulant materials to give the highest stability and prebiotic potential of the colorant (betacyanin) in red dragon fruit peel. The stability and prebiotic potential tests were conducted on the encapsulates during 15 days of storage at room temperature in a dark room. The treatment variations included different maltodextrin to gelatin (GM) ratios of 1:1 (GM1), 2:1 (GM2), and 3:1 (GM3). The parameters tested were encapsulation yield, moisture content, betacyanin content, percent inhibition of free radicals, and prebiotic potential using probiotic Lactobacillus bulgaricus (L. bulgaricus). The results showed that encapsulation could maintain the stability of betacyanin pigment during storage. GM1 gave the best overall results with 10.09% moisture content, 93.94% encapsulate yield, 70% solubility, 0.95 mg/L betacyanin content, 36.31% inhibition percentage, and 5.83 x 107 CFU/mL L. bulgaricus growth.

Cadmium Accumulation and Tolerance of Talinum paniculatum Callus Culture Miranda Gardha Viorenta; Ratih Restiani; Dwi Aditiyarini
Jurnal Biodjati Vol 10 No 1 (2025): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v10i1.38255

The increasing use of inorganic fertilizers is one of the main factors contributing to increased Cadmium (Cd) pollution in the environment. Phytoremediation is one of the strategies that can be used to address the problem of Cd pollution in the environment. The selection of Cadmium-tolerant plants can be conducted using an in vitro culture. Talinum paniculatum as an ornamental plant is potentially used as a phytoremediation agent, but limited information is available regarding its accumulation ability and tolerance to cadmium stress. Therefore, this study aims to determine the accumulation ability and tolerance of T. paniculatum callus to cadmium at various concentrations. This study used a completely randomized design (CRD) with six replications of Cd concentration treatment (0, 5, 10, and 20 ppm). Accumulation and tolerance of callus to Cd were assessed based on callus biomass, callus color, tolerance index, heavy metal concentration in callus and media, and Bioconcentration Factor value. An increase in Cd concentration showed a change in callus color from yellowish green to blackish. In addition, there was no significant difference in dry weight (0.078-0.086 g) and tolerance index (102.631%-113.158%) of callus. However, increasing Cd concentration showed significant differences in callus Cd accumulation from media (5-20 ppm) and Bioconcentration Factor (BCF) ratio (1.282-5.701), indicating the ability of T. paniculatum as an accumulator plant. This study's results support phytoremediation efforts against heavy metal pollution, including cadmium.

Antibacterial Activity of Dendrophthoe pentandra (L.) Leaves Extract Against Staphylococcus aureus and Escherichia coli Inggrid, Annabelle Marie; Aditiyarini, Dwi; Prakasita, Vinsa Cantya
Jurnal Biodjati Vol 9 No 2 (2024): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v9i2.30436

Sapuran, Wonosobo is a popular area with several local tea plantations, which are infested by mistletoe, such as Dendrophthoe pentandra (L.) Miq. Mistletoe has been widely reported to have antibacterial activity due to its secondary metabolite content. Therefore, this study aimed to identify and explore the antibacterial activity of D. pentandra leaves extract against Staphylococcus aureus and Escherichia coli. The test samples were extracted using the maceration method, followed by phytochemical screening and total assay of flavonoids, phenols, and tannins. Antibacterial activity tests were carried out using disk diffusion, Minimum Inhibitory Concentration (MIC), and Minimum Bactericidal Concentration (MBC). The yield from the maceration method using 96% ethanol solvent was 8.78%, containing secondary metabolite compounds, including alkaloids, saponins, steroids, terpenoids, and flavonoids. The total flavonoid, phenol, and tanin content obtained were 4.09 Â± 0.70 mg QE/g, 1.76 Â± 0.16 g GAE/g, and 1.37 Â± 0.14 mg TAE/g, respectively. In addition, the extract showed medium inhibition of S. aureus at a concentration of 400 and 525 mg/m, with no inhibitory effect against E. coli. The MIC and MBC from the microdilution method for S. aureus were at 4400 and 8800 mg/mL extract, while values of 28000 and 56000 mg/mL were obtained for E. coli. Based on these results, the inhibition activity of ethanol extract of D. pentandra against S. aureus was greater compared to E. coli.

Antibacterial Activity of Scurrula ferruginea (Roxb. Ex Jack) Leaves Extract Against Staphylococcus aureus and Escherichia coli Aditiyarini, Dwi; Sipayung, Nita Sonia; Prakasita, Vinsa Cantya
Jurnal Biodjati Vol 9 No 1 (2024): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v9i1.30445

The mistletoe (Scurrula ferruginea) is a parasitic plant obtaining nutrients and water from host plants at Nglinggo tea plantation, Kulon Progo, Yogyakarta. Despite the numerous benefits of Scurrula ferruginea leaf, substantiation regarding its pharmacological activity is still lacking. Therefore, this research aimed to identify Scurrula ferruginea species and obtain secondary metabolites with antibacterial activity. The experiment was carried out by extracting Scurrula ferruginea leaf using maceration methods with 96% ethanol, followed by qualitatively and quantitatively phytochemical screening, inhibitory zone test, minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) test. Several compounds, such as flavonoids, tannins, phenols, saponins, alkaloids, terpenoids, and steroids, were identified in the mistletoe extract. The results showed total flavonoid, phenolic, and tannin levels were 7.41 Â± 0.38 mg QE/mg, 1672 Â± 50.99 mg GAE/g, and 1.43 Â± 0.15 mg TAE/g, respectively. Scurrula ferruginea leaf ethanolic extract showed the potential to form an inhibition zone against S. aureus in the moderate category at 525 mg/mL, but no significant activity was observed against E. coli. The MIC results of Scurrula ferruginea leaf ethanolic extract against S. aureus were 0.6%, and the MBC was at 2.4%. Furthermore, the MIC value against E. coli was 14%, and the MBC value was 28%. Based on these results, it could be concluded that Scurrula ferruginea leaf ethanolic extract showed high effectiveness in inhibiting and killing S. aureus bacteria compared to E. coli, with MIC and MBC values of 0.6% and 2.4%, respectively

Antidiabetic Potential of Ethanol Extract from Moringa oleifera Leaves in Streptozotocin-Nicotinamide-Induced Female Mus musculus Prakasita, Vinsa Cantya; Theodora, Nadya Aprina; Susilowati, Enjelin Anjung; Aditiyarini, Dwi; Prasetyaningsih, Aniek
Jurnal Biodjati Vol 9 No 1 (2024): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v9i1.30699

Type 2 diabetes mellitus (DM) is a chronic metabolic disÂorder characterized by elevated blood glucose levels due to insulin resistance. Type 2 diabetes is considerably more prevalent than othÂer forms (85â€“90%). The risk of type 2 diabetes is higher in women (53.2%). There is an urgent requirement for better and more affordaÂble treatment options considering DM therapy is expensive and may have adverse health effects. The study's objective is to examine how acute toxicity, blood glucose levels, and body weight are affected by Moringa leaf ethanol extract (MLEE). Maceration was employed to eliminate the leaves of the moringa plant. Phytochemical screening was completed to assess the total flavonoid content and screen for alkaloids, flavonoids, phenolics, saponins, and tannins. Acute toxicity testing was performed following OECD guideline 423. Clinical sympÂtoms of acute toxicity were observed every 30 minutes for the first 24 hours post-treatment, followed by observations every 24 hours up to 14 days. The estimated LD50 range was determined. Streptozotocin/ nicotinamide-induced female Mus musculus was administered to evalÂuate the antidiabetic potential of MLEE. Six groups of mice were utiÂlized, which included a healthy control group (aquades not induced), a negative control group (induced aquades), a positive control group (induced glimepiride 0.8 mg/kg BW), and three treatment groups with varying dosages of MLEE (induced; 0, 100, and 150 mg/kg BW). A semi-auto chemical analyzer was employed on days 0 through 31 to determine blood glucose levels. An analytical digital balance was utilized to calculate the body weight. With a total flavonoid concenÂtration of 20.75%, MLEE incorporated alkaloids, flavonoids, phenoÂlics, saponins, and tannins. MLEE demonstrated a significant effect in lowering blood glucose levels at a dose of 100 mg/kg BW (P<0.05). A significant positive correlation has been identified between body weight and blood glucose levels (P<0.05).

Effect of Plant Growth Regulators (PGRs) on Biomass and Flavonoid Production of Talinum paniculatum Callus Culture Kaban, Sarah Mega Pratenna; Restiani, Ratih; Aditiyarini, Dwi
Jurnal Biodjati Vol 9 No 1 (2024): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v9i1.33788

Talinum paniculatum Jacq (Gaertn), locally known as Javanese Ginseng, is an essential medicinal plant characterized by high flavonoid content with antioxidant and antimicrobial activities. Compared to other natural sources, a larger number of T. paniculatum leaves is required to obtain high amounts of flavonoid for drug development. In this context, in vitro culture can be used to increase flavonoid production in a controlled condition without reducing plant population. The concentration of Plant Growth Regulators (PGRs) is capable of influencing biomass and flavonoid accumulation in callus culture from several species. Despite these benefits, there is limited information about the optimal concentration of PGRs for flavonoid production from T. paniculatum callus culture. Therefore, this study aimed to investigate the optimum concentration of 2,4-D and kinetin for callus growth, as well as flavonoid production from T. paniculatum. The investigation was carried out using a completely randomized design (CRD), where young leaves explants were cultured on MS media supplemented with various concentrations of 2,4-D (0, 0.5, 1, 2) mg/L and kinetin (0, 0.5, 1, 2) mg/L for callus and flavonoid production. The results showed that the highest callus biomass of 0.105 g was produced from MS medium with 2 mg/L of 2,4-D and 1 mg/L of kinetin. Meanwhile, the highest total flavonoid content of 25.66 mg QE/g DW was produced from media supplemented with 1 mg/Lof 2,4-D and 2 mg/L of kinetin. These results showed that different combinations of 2,4-D and kinetin were required to increase biomass and flavonoid production. In conclusion, this study provided valuable information for the development of bioactive compounds through in vitro culture.

Stabilitas Ekstrak Bunga Telang (Clitoria ternatea L.) Terenkapsulasi Maltodekstrin dan Gelatin dan Potensi Prebiotiknya Sari, Devina Puspita; Aditiyarini, Dwi; Ariestanti, Catarina Aprilia
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 9, No 3 (2024): October 2024
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v9i3.7550

Bunga telang merupakan salah satu bunga yang dapat dimakan dan digunakan sebagai pewarna alami makanan. Bunga ini juga berpotensi sebagai antioksidan dan prebiotik akibat kandungan antosianinnya. Namun ekstrak bunga telang mudah terdegradasi. Stabilitas zat warna bunga telang dapat dipertahankan dengan teknik enkapsulasi menggunakan maltodekstrin dan gelatin. Penelitian ini dilakukan untuk mengetahui rasio maltodekstrin dan gelatin yang menghasilkan stabilitas enkapsulat bunga telang terbaik selama 15 hari penyimpanan dan potensi prebiotiknya. Ekstrak bunga telang diperoleh melalui metode maserasi dengan pelarut etanol 70%. Rasio maltodekstrin dan gelatin yang digunakan adalah 1:1, 3:1 dan 5:1, dengan kadar ekstrak bunga telang 1%. Stabilitas enkapsulat dianalisis dari kandungan antosianin dan antioksidan dengan waktu uji setiap 5 hari selama 15 hari. Kadar antosianin diukur dengan metode pH differential. Aktivitas antioksidan diukur dengan metode DPPH. Potensi prebiotik diuji menggunakan Lactobacillus bulgaricus. Hasil menunjukkan bahwa enkapsulat ekstrak bunga telang dengan rasio maltodekstrin dan gelatin sebesar 3:1 memiliki stabilitas enkapsulat terbaik dengan kadar air 6,863%, kadar antosianin 0,217 mg/g, dan daya redam radikal bebas sebesar 54,858%. Enkapsulat ini juga terbukti berpotensi menjadi prebiotik yang ditandai dengan pertumbuhan L.bulgaricus sebesar 2,85 x 109 CFU/mL.

Aktivitas Antibakteri Ekstrak Kulit Batang Belimbing Wuluh Terhadap Escherichia coli Penyebab Diare Septhimoranie, Jade; Aditiyarini, Dwi; Prakasita, Vinsa Cantya
Bioeksperimen: Jurnal Penelitian Biologi Vol 8, No 2: September 2022
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v8i2.15749

Diare merupakan salah satu gejala infeksi pada saluran cerna, yang dapat disebabkan oleh adanya organisme (bakteri, virus, dan/atau parasit), sehingga menyebabkan feses menjadi lebih cair dan frekuensinya lebih sering dari biasanya. Escherichia coli memiliki presentase yang tinggi dibandingkan bakteri lain pada feses penderita diare. Pengobatan yang dilakukan biasanya dengan menggunakan antibiotik, namun sekarang ini banyak ditemukan antibiotik yang telah resisten. Tanaman obat-obatan dapat digunakan sebagai alternatif dalam mengatasi diare. Salah satunya ialah kulit batang Belimbing Wuluh. Adanya kandungan senyawa yang bersifat antibakteri pada kulit batang Belimbing Wuluh dimungkinkan dapat menghambat pertumbuhan E. coli penyebab diare. Tujuan penelitian adalah untuk mengetahui aktivitas antibakteri ekstrak kulit batang Belimbing Wuluh terhadap E. coli penyebab diare. Pada penelitian ini menggunakan metode ekstraksi dekoktasi. Uji fitokimia dilakukan terhadap flavonoid, alkaloid, dan saponin. Uji total fenol dilakukan dengan menggunakan metode Folin-Ciocalteu. Uji antibakteri dilakukan dengan menggunakan disk diffusion method Kirby-Bauer, dilanjutkan uji MIC dengan metode mikrodilusi serta uji MBC. Analisis data dengan One-Way ANOVA dan post-hoc Duncan. Kulit batang Belimbing Wuluh mengandung senyawa saponin, alkaloid, flavonoid, dan fenol. Hasil rata-rata dari nilai total fenol adalah 25,12 ± 7,73(mg GAE)⁄g. Hasil uji aktivitas antibakteri menunjukkan bahwa ekstrak kulit batang Belimbing Wuluh dengan konsentrasi 2,5% masih dapat menghambat E. coli secara signifikan (P≤0,05).

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