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Analysis of secondary metabolites and antioxidant activities of ethanol extract of Dendrophthoe pentandra (L.) Miq.) in Sapuran, Central Java Haryono, Sevien Elamaria; Aditiyarini, Dwi; Restiani, Ratih
Biogenesis: Jurnal Ilmiah Biologi Vol 12 No 1 (2024)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v12i1.40323

Abstract

Dendrophthoe pentandra (L.) Miq. are known to have the potential for health because they can be used as natural medicines to cure several diseases, including measles, coughs, diuretics, pain relievers, anti-inflammatory medications, as well as wound healing and infections. The local community in Indonesia has believed the health beneficial of tea mistletoe, but the information of its pharmacological activity is still limited. This research was conducted to explore the species and diversity of secondary metabolites of D. pentandra (L.) Miq. found in Sapuran, Wonosobo and its antioxidant activity. Extraction was performed by maceration in 96% ethanol. It is followed by phytochemical screening, determination of total secondary metabolite compounds, and antioxidant activity assay using DPPH. The results of this study confirmed that mistletoe is D. pentandra (L.). Its leaf extract has a yield of 8.76% that contains alkaloids, saponins, tannins, steroids, phenols, and terpenoids. Its total content of flavonoids, tannins, phenols and saponins was, respectively, 48.584 mg QE/g extract, 1.350 ± 0.140 mg TAE/g extract, 1.756 ± 0.171 g GAE/g extract, 4.665 ± 0.142 g SE/g extract. Its ethanolic extract has very high antioxidant activity with an IC50 value of 49.10 ppm. D. pentandra (L.) Miq. of Sapuran, Wonosobo, has great potential to be developed in the pharmacological field.
The Effect of Callus Age on Biomass and Flavonoid Production in Talinum paniculatum In Vitro Culture Restiani, Ratih; Aditiyarini, Dwi; Dolonseda, Anggel Christia; Nathania, Yohana Elsa
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 10, No 1: Jurnal Pembelajaran Dan Biologi Nukleus March 2024
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v10i1.5417

Abstract

Talinum paniculatum (Javanese Ginseng) is an herbaceous plant belonging to Talinaceae family that has been known as an important medicinal plant. Flavonoid is one of the prominent bioactive compounds that are contained in all parts of T. paniculatum and has been known for its several bioactivities, including antioxidant, anti-inflammatory, antimicrobial, antidiabetic, and anti-cancer. Callus culture can be used to ensure the quantity and quality of flavonoid production. Callus age is one of the important factors that needs to be considered in order to produce optimum biomass and flavonoid production. Therefore, this research aimed to investigate the effect of callus age on biomass and flavonoid production in T. panicultaum in vitro culture. Young leaves were used as an explant, and callus induction was performed using MS solid media supplemented with kinetin 3 mg/L and 2.4-D 2 mg/L. Biomass and flavonoid production were observed during 0, 28, 35, 42, and 49 days. Results showed that biomass and flavonoid production were influenced by callus age. The production of flavonoid in T. paniculatum callus culture was not linear with the growth of callus (biomass), and the optimum flavonoid production resulted in the 28th days of callus age (49.100 mg QE/g), while the optimum biomass resulted in the 49th days of callus age (4.295 g). It can be concluded that the optimum conditions for biomass and flavonoid production in T. paniculatum callus culture are influenced by different callus ages
Stabilitas Ekstrak Bunga Telang (Clitoria ternatea L.) Terenkapsulasi Maltodekstrin dan Gelatin dan Potensi Prebiotiknya Sari, Devina Puspita; Aditiyarini, Dwi; Ariestanti, Catarina Aprilia
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 9, No 3 (2024): October 2024
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v9i3.7550

Abstract

Bunga telang merupakan salah satu bunga yang dapat dimakan dan digunakan sebagai pewarna alami makanan. Bunga ini juga berpotensi sebagai antioksidan dan prebiotik akibat kandungan antosianinnya. Namun ekstrak bunga telang mudah terdegradasi. Stabilitas zat warna bunga telang dapat dipertahankan dengan teknik enkapsulasi menggunakan maltodekstrin dan gelatin. Penelitian ini dilakukan untuk mengetahui rasio maltodekstrin dan gelatin yang menghasilkan stabilitas enkapsulat bunga telang terbaik selama 15 hari penyimpanan dan potensi prebiotiknya. Ekstrak bunga telang diperoleh melalui metode maserasi dengan pelarut etanol 70%. Rasio maltodekstrin dan gelatin yang digunakan adalah 1:1, 3:1 dan 5:1, dengan kadar ekstrak bunga telang 1%. Stabilitas enkapsulat dianalisis dari kandungan antosianin dan antioksidan dengan waktu uji setiap 5 hari selama 15 hari. Kadar antosianin diukur dengan metode pH differential. Aktivitas antioksidan diukur dengan metode DPPH. Potensi prebiotik diuji menggunakan Lactobacillus bulgaricus. Hasil menunjukkan bahwa enkapsulat ekstrak bunga telang dengan rasio maltodekstrin dan gelatin sebesar 3:1 memiliki stabilitas enkapsulat terbaik dengan kadar air 6,863%, kadar antosianin 0,217 mg/g, dan daya redam radikal bebas sebesar 54,858%. Enkapsulat ini juga terbukti berpotensi menjadi prebiotik yang ditandai dengan pertumbuhan L.bulgaricus sebesar 2,85 x 109 CFU/mL.
Effect of Fermentation Time on the Antioxidant and Antibacterial Activity of Fermented Cascara Beverage Simanjuntak, Monayanti; Hutabarat, Diva Yolanda; Sutan, Ditha Klarissa; Harjanto, Eunike; Aditiyarini, Dwi
Journal of Applied Food Technology Vol 11, No 2 (2024)
Publisher : Dept. Food Technology, Faculty of Animal and Agricultural Sciences, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17728/jaft.24038

Abstract

Indonesia is a country with a high consumption of coffee, which is in direct comparison with the increased production of coffee waste. Arabica coffee husk (cascara) is coffee industry waste that has not been utilized optimally, which is only 0.76% of the existing potential. Cascara is known to have a secondary metabolite compound that can be utilized as a good product for human health. The effects of Arabica cascara fermentation time on the antioxidant and antibacterial activity of cascara beverage were studied in this study. This research is using a completely randomized non-factorial design. Cascara was obtained from Puntang Mountain, West Java, and was fermented with Lactiplantibacillus plantarum (previously known as Lactobacillus plantarum) for 10, 14, and 18 days. pH value was measured with a pH meter, total sugar content with phenol sulfuric acid method, caffeine content with high performance liquid chromatography (HPLC) method, total lactic acid bacteria (LAB) with drop plate method, total flavonoid and phenolic content with UV-Vis spectrophotometry method, antioxidant activity with 2,2-diphenyl-1-picrylhydrazyl (DPPH) method, and antibacterial activity against Helicobacter pylori with disc diffusion method. Data were analyzed using One-Way ANOVA and DMRT test at the significance level of 0.05. The results showed that prolonged fermentation times reduce the quality of Arabica cascara beverage. The optimal fermentation times were found to be 10 days producing the best balance of pH (3.66), total sugar content (1.29%), caffeine content (0.043%), total LAB (1 x 107), total phenols (12.74 ppm), total flavonoids (3.40 ppm) and highest antioxidant IC50 activity (215 ppm) while for antibacterial activity has not been able to inhibit the growth of Helicobacter pylori.
Antibacterial Activity of Dendrophthoe pentandra (L.) Leaves Extract Against Staphylococcus aureus and Escherichia coli Inggrid, Annabelle Marie; Aditiyarini, Dwi; Prakasita, Vinsa Cantya
Jurnal Biodjati Vol 9, No 2 (2024): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v9i2.30436

Abstract

Sapuran, Wonosobo is a popular area with several local tea plantations, which are infested by mistletoe, such as Dendrophthoe pentandra (L.) Miq. Mistletoe has been widely reported to have antibacterial activity due to its secondary metabolite content. Therefore, this study aimed to identify and explore the antibacterial activity of D. pentandra leaves extract against Staphylococcus aureus and Escherichia coli. The test samples were extracted using the maceration method, followed by phytochemical screening and total assay of flavonoids, phenols, and tannins. Antibacterial activity tests were carried out using disk diffusion, Minimum Inhibitory Concentration (MIC), and Minimum Bactericidal Concentration (MBC). The yield from the maceration method using 96% ethanol solvent was 8.78%, containing secondary metabolite compounds, including alkaloids, saponins, steroids, terpenoids, and flavonoids. The total flavonoid, phenol, and tanin content obtained were 4.09 ± 0.70 mg QE/g, 1.76 ± 0.16 g GAE/g, and 1.37 ± 0.14 mg TAE/g, respectively. In addition, the extract showed medium inhibition of S. aureus at a concentration of 400 and 525 mg/m, with no inhibitory effect against E. coli. The MIC and MBC from the microdilution method for S. aureus were at 4400 and 8800 mg/mL extract, while values of 28000 and 56000 mg/mL were obtained for E. coli. Based on these results, the inhibition activity of ethanol extract of D. pentandra against S. aureus was greater compared to E. coli.
Stability of encapsulated red dragon fruit (Hylocereus polyrhizus) peel: effects on antioxidant and prebiotic potency Justina, Fayola; Aditiyarini, Dwi; Ariestanti, Catarina Aprilia
Advances in Food Science, Sustainable Agriculture and Agroindustrial Engineering (AFSSAAE) Vol 7, No 4 (2024)
Publisher : Advances in Food Science, Sustainable Agriculture and Agroindustrial Engineering (AFSSAAE)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.afssaae.2024.007.04.1

Abstract

The high consumption of red dragon fruit has led to an increase in red dragon fruit peel waste in the environment, which has potential as a source of natural food coloring and as a prebiotic but is susceptible todegraded. The objective of this study was to determine the best ratio of maltodextrin and gelatin as encapsulant materials to give the highest stability and prebiotic potential of the colorant (betacyanin) in red dragon fruit peel. The stability and prebiotic potential tests were conducted on the encapsulates during 15 days of storage at room temperature in a dark room. The treatment variations included different maltodextrin to gelatin (GM) ratios of 1:1 (GM1), 2:1 (GM2), and 3:1 (GM3). The parameters tested were encapsulation yield, moisture content, betacyanin content, percent inhibition of free radicals, and prebiotic potential using probiotic Lactobacillus bulgaricus (L. bulgaricus). The results showed that encapsulation could maintain the stability of betacyanin pigment during storage. GM1 gave the best overall results with 10.09% moisture content, 93.94% encapsulate yield, 70% solubility, 0.95 mg/L betacyanin content, 36.31% inhibition percentage, and 5.83 x 107 CFU/mL L. bulgaricus growth.
Cadmium Accumulation and Tolerance of Talinum paniculatum Callus Culture Miranda Gardha Viorenta; Ratih Restiani; Dwi Aditiyarini
Jurnal Biodjati Vol 10 No 1 (2025): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v10i1.38255

Abstract

The increasing use of inorganic fertilizers is one of the main factors contributing to increased Cadmium (Cd) pollution in the environment. Phytoremediation is one of the strategies that can be used to address the problem of Cd pollution in the environment. The selection of Cadmium-tolerant plants can be conducted using an in vitro culture. Talinum paniculatum as an ornamental plant is potentially used as a phytoremediation agent, but limited information is available regarding its accumulation ability and tolerance to cadmium stress. Therefore, this study aims to determine the accumulation ability and tolerance of T. paniculatum callus to cadmium at various concentrations. This study used a completely randomized design (CRD) with six replications of Cd concentration treatment (0, 5, 10, and 20 ppm). Accumulation and tolerance of callus to Cd were assessed based on callus biomass, callus color, tolerance index, heavy metal concentration in callus and media, and Bioconcentration Factor value. An increase in Cd concentration showed a change in callus color from yellowish green to blackish. In addition, there was no significant difference in dry weight (0.078-0.086 g) and tolerance index (102.631%-113.158%) of callus. However, increasing Cd concentration showed significant differences in callus Cd accumulation from media (5-20 ppm) and Bioconcentration Factor (BCF) ratio (1.282-5.701), indicating the ability of T. paniculatum as an accumulator plant. This study's results support phytoremediation efforts against heavy metal pollution, including cadmium.
Antibacterial Activity of Dendrophthoe pentandra (L.) Leaves Extract Against Staphylococcus aureus and Escherichia coli Inggrid, Annabelle Marie; Aditiyarini, Dwi; Prakasita, Vinsa Cantya
Jurnal Biodjati Vol 9 No 2 (2024): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v9i2.30436

Abstract

Sapuran, Wonosobo is a popular area with several local tea plantations, which are infested by mistletoe, such as Dendrophthoe pentandra (L.) Miq. Mistletoe has been widely reported to have antibacterial activity due to its secondary metabolite content. Therefore, this study aimed to identify and explore the antibacterial activity of D. pentandra leaves extract against Staphylococcus aureus and Escherichia coli. The test samples were extracted using the maceration method, followed by phytochemical screening and total assay of flavonoids, phenols, and tannins. Antibacterial activity tests were carried out using disk diffusion, Minimum Inhibitory Concentration (MIC), and Minimum Bactericidal Concentration (MBC). The yield from the maceration method using 96% ethanol solvent was 8.78%, containing secondary metabolite compounds, including alkaloids, saponins, steroids, terpenoids, and flavonoids. The total flavonoid, phenol, and tanin content obtained were 4.09 ± 0.70 mg QE/g, 1.76 ± 0.16 g GAE/g, and 1.37 ± 0.14 mg TAE/g, respectively. In addition, the extract showed medium inhibition of S. aureus at a concentration of 400 and 525 mg/m, with no inhibitory effect against E. coli. The MIC and MBC from the microdilution method for S. aureus were at 4400 and 8800 mg/mL extract, while values of 28000 and 56000 mg/mL were obtained for E. coli. Based on these results, the inhibition activity of ethanol extract of D. pentandra against S. aureus was greater compared to E. coli.
Antibacterial Activity of Scurrula ferruginea (Roxb. Ex Jack) Leaves Extract Against Staphylococcus aureus and Escherichia coli Aditiyarini, Dwi; Sipayung, Nita Sonia; Prakasita, Vinsa Cantya
Jurnal Biodjati Vol 9 No 1 (2024): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v9i1.30445

Abstract

The mistletoe (Scurrula ferruginea) is a parasitic plant obtaining nutrients and water from host plants at Nglinggo tea plantation, Kulon Progo, Yogyakarta. Despite the numerous benefits of Scurrula ferruginea leaf, substantiation regarding its pharmacological activity is still lacking. Therefore, this research aimed to identify Scurrula ferruginea species and obtain secondary metabolites with antibacterial activity. The experiment was carried out by extracting Scurrula ferruginea leaf using maceration methods with 96% ethanol, followed by qualitatively and quantitatively phytochemical screening, inhibitory zone test, minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) test. Several compounds, such as flavonoids, tannins, phenols, saponins, alkaloids, terpenoids, and steroids, were identified in the mistletoe extract. The results showed total flavonoid, phenolic, and tannin levels were 7.41 ± 0.38 mg QE/mg, 1672 ± 50.99 mg GAE/g, and 1.43 ± 0.15 mg TAE/g, respectively. Scurrula ferruginea leaf ethanolic extract showed the potential to form an inhibition zone against S. aureus in the moderate category at 525 mg/mL, but no significant activity was observed against E. coli. The MIC results of Scurrula ferruginea leaf ethanolic extract against S. aureus were 0.6%, and the MBC was at 2.4%. Furthermore, the MIC value against E. coli was 14%, and the MBC value was 28%. Based on these results, it could be concluded that Scurrula ferruginea leaf ethanolic extract showed high effectiveness in inhibiting and killing S. aureus bacteria compared to E. coli, with MIC and MBC values of 0.6% and 2.4%, respectively
Antidiabetic Potential of Ethanol Extract from Moringa oleifera Leaves in Streptozotocin-Nicotinamide-Induced Female Mus musculus Prakasita, Vinsa Cantya; Theodora, Nadya Aprina; Susilowati, Enjelin Anjung; Aditiyarini, Dwi; Prasetyaningsih, Aniek
Jurnal Biodjati Vol 9 No 1 (2024): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v9i1.30699

Abstract

Type 2 diabetes mellitus (DM) is a chronic metabolic dis­order characterized by elevated blood glucose levels due to insulin resistance. Type 2 diabetes is considerably more prevalent than oth­er forms (85–90%). The risk of type 2 diabetes is higher in women (53.2%). There is an urgent requirement for better and more afforda­ble treatment options considering DM therapy is expensive and may have adverse health effects. The study's objective is to examine how acute toxicity, blood glucose levels, and body weight are affected by Moringa leaf ethanol extract (MLEE). Maceration was employed to eliminate the leaves of the moringa plant. Phytochemical screening was completed to assess the total flavonoid content and screen for alkaloids, flavonoids, phenolics, saponins, and tannins. Acute toxicity testing was performed following OECD guideline 423. Clinical symp­toms of acute toxicity were observed every 30 minutes for the first 24 hours post-treatment, followed by observations every 24 hours up to 14 days. The estimated LD50 range was determined. Streptozotocin/ nicotinamide-induced female Mus musculus was administered to eval­uate the antidiabetic potential of MLEE. Six groups of mice were uti­lized, which included a healthy control group (aquades not induced), a negative control group (induced aquades), a positive control group (induced glimepiride 0.8 mg/kg BW), and three treatment groups with varying dosages of MLEE (induced; 0, 100, and 150 mg/kg BW). A semi-auto chemical analyzer was employed on days 0 through 31 to determine blood glucose levels. An analytical digital balance was utilized to calculate the body weight. With a total flavonoid concen­tration of 20.75%, MLEE incorporated alkaloids, flavonoids, pheno­lics, saponins, and tannins. MLEE demonstrated a significant effect in lowering blood glucose levels at a dose of 100 mg/kg BW (P<0.05). A significant positive correlation has been identified between body weight and blood glucose levels (P<0.05).