Article Per Year (5 Year)
p-Index From 2021 - 2026
3.713
P-Index
This Author published in this journals
All Journal METAMORFOSA Journal of Biological Sciences Biotika: Jurnal Ilmiah Biologi Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Biogenesis: Jurnal Ilmiah Biologi Prosiding Seminar Biologi Edumatsains Jurnal Biodjati Jurnal Biologi Tropis Biosaintifika: Journal of Biology & Biology Education Jurnal Pembelajaran dan Biologi Nukleus Jurnal Pembelajaran Pemberdayaan Masyarakat (JP2M) Sciscitatio : Journal for Biological Science Jurnal Pengabdian kepada Masyarakat Nusantara JURNAL BIOLOGI PAPUA SERVIRISMA: Jurnal Pengabdian kepada Masyarakat Pelita: Jurnal Pengabdian kepada Masyarakat
Ratih Restiani
Program Studi Biologi, Fakultas Bioteknologi, Universitas Kristen Duta Wacana
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Mathematics Physics Social Sciences Other

Published : 28 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 28 Documents
Search

 1   2   3 
Profiling secondary metabolites and antioxidant activity of tea mistletoe leaves (Scurrula artopurpurea (Bl.) Danser) in Nglinggo, Kulon Progo, Yogyakarta Dwi Aditiyarini; Ratih Restiani; Evieyana Evieyana
Biogenesis: Jurnal Ilmiah Biologi Vol 10 No 2 (2022)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v10i2.31258

Abstract

.
Pengaruh Konsentrasi Sukrosa terhadap Produksi Flavonoid pada Kultur Kalus Ginseng Jawa (Talinum paniculatum (Jacq.) Gaertn.) Josiah Herald Matheos; Ratih Restiani; Dwi Adityarini
BIOTIKA Jurnal Ilmiah Biologi Vol 20, No 2 (2022): BIOTIKA DESEMBER 2022
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/biotika.v20i2.41419

Abstract

Ginseng Jawa (Talinum paniculatum (Jacq.) Gaertn.) merupakan tanaman obat dengan berbagai manfaat karena memiliki metabolit sekunder yang beragam seperti saponin, tanin, triterpenoid, hingga flavonoid. Flavonoid merupakan salah satu metabolit sekunder yang dominan dihasilkan oleh daun Ginseng Jawa. Flavonoid dapat ditingkatkan produksinya melalui kultur in vitro dengan penambahan sukrosa pada konsentrasi optimal. Penelitian ini bertujuan untuk mengetahui pengaruh penambahan sukrosa pada berbagai konsentrasi terhadap peningkatan biomassa dan produksi flavonoid kalus. Desain yang digunakan dalam penelitian adalah Rancangan Acak Lengkap (RAL) dengan faktor tunggal yaitu konsentrasi sukrosa (0 ; 10 ; 20 ; 30 dan 40 g/L) sebanyak 5 ulangan. Kalus diinisiasi dari eksplan daun yang dikulturkan dalam media MS dengan penambahan 3 mg/L Kinetin + 2 mg/L 2,4-D + variasi konsentrasi sukrosa. Kalus dipanen pada hari ke-28, dikeringkan menggunakan oven pada suhu 400 C sampai diperoleh berat kering konstan kemudian diekstraksi menggunakan metanol 96% dengan metode maserasi. Hasil menunjukkan bahwa konsentrasi sukrosa 30 g/L dapat meningkatkan biomassa kalus secara optimal (0,081 g) dan konsentrasi sukrosa 40 g/L meningkatkan kandungan flavonoid total (TFC) sebesar 19,04 mg QE/g berat kering kalus. Hasil penelitian ini berperan penting sebagai informasi dasar mengenai kondisi optimal untuk peningkatan biomassa dan produksi flavonoid melalui kultur kalus T.paniculatum.
Browning Prevention Method in Kepel (Stelechocarpus burahol (Blume) Hook.f. & Thomson) in Vitro Culture Devi Sonti Sibarani; Ratih Restiani; Aniek Prasetyaningsih
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS (JPBN) Vol 10, No 1 (2024): Jurnal Pembelajaran Dan Biologi Nukleus March 2024
Publisher : Fakultas Keguruan dan Ilmu Pendidikan Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v10i1.5464

Abstract

Browning of explants is a common problem in Kepel (Stelechocarpus burahol (Blume) Hook.f. & Thomson) in vitro culture, resulting in low explant in vitro regeneration of Kepel. Using nodal explant, the effect of explant immersion in ascorbic acid, the addition of ascorbic acid to the media, and dark and light incubation conditions were investigated. The browning prevention method was selected based on the delayed browning appearance time, the lowest broning intensity, and the highest percentage of callus. This present study used a completely randomized design with treatment variations: immersion explant in ascorbic acid 100 mg/L, addition of ascorbic acid 100 and 200 mg/L to the media, addition of ascorbic acid 100 and 200 mg/L and activated charcoal 1 g/L to the media, and incubation in dark and light conditions for 28 days. The results showed that the combination of the addition of ascorbic acid 200 mg/L to MS media and incubation in dark conditions were effective browning prevention methods in inhibiting browning appearance time (5 DAP), significantly reducing browning intensity (0.3), and increasing callus growth (100%) of kepel node explants during 28 days of culture. The results of this study are useful in establishing protocols for in vitro culture of Kepel plants, especially at the initiation stage, and can be expected to support the successful conservation of Kepel plants through in vitro propagation. Keywords: Ascorbic acid, Activated Charcoal, Browning, In Vitro Culture, Stelechocarpus burahol
INDUKSI KALUS DARI EKSPLAN NODUS Stelecocharpus burahol (Blume) Hook. f & Thomson SEBAGAI UPAYA KONSERVASI IN VITRO Sekar, Astrid Ayu; Restiani, Ratih; Prasetyaningsih, Aniek
BIOTIKA Jurnal Ilmiah Biologi Vol 21, No 1 (2023): BIOTIKA JUNI 2023
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/biotika.v21i1.42869

Abstract

Tanaman Kepel (Stelechocarpus burahol (Blume) Hook. f. & Thomson) merupakan salah satu jenis tanaman asli Indonesia yang mengandung metabolit sekunder dan potensial sebagai antioksidan, antibakteri, antifungi, anti inflamasi, dan anti implantasi. Namun, saat ini tanaman kepel berstatus conservation dependent, sehingga diperlukan perbanyakan tanaman kepel melalui kultur in vitro. Penelitian ini bertujuan untuk menghasilkan metode perbanyakan tanaman kepel secara in vitro melalui tahap induksi kalus dengan mengoptimasi kombinasi dan konsentrasi BAP (Benzylaminopurin) dan IAA (Indole-3-acetic acid). Penelitian ini menggunakan desain Rancangan Acak Lengkap (RAL) satu faktor dengan kombinasi konsentrasi BAP dan IAA (0,1,2,5, dan 5 mgL-1 ) sebanyak 16 perlakuan masing-masing 3 ulangan. Eskplan nodus yang ditanam dalam medium MS dengan penambahan BAP dan IAA dikultur pada suhu 25 ± 2 0C, kondisi terang 24 jam dengan intensitas cahaya 3000 flux selama 30 hari. Pengamatan waktu induksi kalus, persentase pertumbuhan kalus dan intensitas kalus dilakukan setiap minggu selama 28 hari masa tanam. Data yang diperoleh dianalisis menggunakan ANOVA dan tidak dilanjutkan uji Duncan karena hasil yang diperoleh tidak berbeda signifikan (p ≥0,05). Hasil menunjukkan bahwa penambahan 1 mgL-1 BAP dan 5 mgL-1 IAAmenghasilkan waktu induksi tercepat yaitu 4,67 ± 1,15 hari, sedangkan medium MS dengan penambahan 5 mgL-1 BAP dan 2,5 mgL-1 IAAmerupakan kombinasi konsentrasi terbaik dalam pembentukan kalus (100%) dan intensitas kalus sebesar 0,57 ± 0,34 dengan tekstur remah berwarna kehijauan. Hasil penelitian ini dapat menjadi informasi penting dalam upaya konservasi tanaman kepel secara in vitro.
Pengaruh Konsentrasi Air Kelapa dan BAP (6-Benzylaminopurin) terhadap Regenerasi In vitro Protokorm Dendrobium stratiotes x Dendrobium wulaiense Agustinus, Vania; Restiani, Ratih; Prasetyaningsih, Aniek
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 9, No 2 (2024): June 2024
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v9i2.7728

Abstract

Kultur in vitro merupakan salah satu solusi alternatif untuk mengatasi kesulitan perbanyakan anggrek melalui biji. Keberhasilan regenerasi protokorm anggrek dipengaruhi oleh penambahan ZPT dan air kelapa ke dalam medium. Oleh karena itu, penelitian ini bertujuan untuk menentukan pengaruh konsentrasi air kelapa dan BAP terhadap regenerasi protokorm Dendrobium hibrida. Penelitian menggunakan Rancangan Acak Lengkap dengan dua faktor yaitu konsentrasi air kelapa dan BAP. Paramater yang diamati meliputi persentase tunas, jumlah tunas, tinggi protokorm, dan persentase akar. Hasil menunjukkan bahwa penambahan air kelapa dan BAP ke dalam medium VW berpengaruh nyata terhadap tinggi dan persentase akar protokorm. Konsentrasi air kelapa (10%) tanpa penambahan BAP dapat meningkatkan persentase tunas, akar, jumlah tunas, dan tinggi protokorm Dendrobium hibrida. Hasil penelitian menunjukan bahwa penggunaan air kelapa secara tunggal maupun kombinasi dengan hormon sitokinin (BAP) signifikan dalam mempercepat regenerasi protokorm Dendrobium hibrida. Ini merupakan penelitian pertama yang melaporkan penggunaan air kelapa dan BAP pada regenerasi protokorm hasil persilangan Dendrobium stratiotes x Dendrobium wulaiense.
Effect of Ascorbic Acid, Activated Charcoal and Dark Incubation on Browning Intensity of Saurauia bracteosa In Vitro Culture Putri Octaviana Ginting, Naomi Bastanta; Restiani, Ratih; Prasetyaningsih, Aniek; Meyga Semarayani, Cokorda Istri
Biosaintifika: Journal of Biology & Biology Education Vol 15, No 3 (2023): December 2023
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v15i3.48439

Abstract

Saurauia bracteosa, also known as Pirdot, is an endemic woody plant of the Actinidiaceae family with medicinal properties. Its population decline in nature has led to a vulnerable status, prompting conservation efforts, such as in vitro culture. Browning poses a significant challenge in the woody plant in vitro culture. Browning inhibitors such as ascorbic acid, activated charcoal and dark incubation can be used to address this issue. This study aims to determine the effect of ascorbic acid, activated charcoal and incubation conditions on browning inhibition of S.bracteosa. The study employed a Completely Randomized Design with 12 treatments on leaf explants, repeated five times. Observations, including the time of browning formation, percentage of browning, intensity of browning and percentage of live explant were carried out for 30 days and analyzed using ANOVA followed by DMRT. The results showed that the addition of activated charcoal in MS media with dark incubation delayed the browning formation time (12.40 DAI) while the adding ascorbic acid in MS media and dark incubation resulted in the lowest browning intensity (0.22). These research findings can serve as a foundational protocol for browning prevention, supporting the successful in vitro conservation of S. bracteosa.
Analysis of secondary metabolites and antioxidant activities of ethanol extract of Dendrophthoe pentandra (L.) Miq.) in Sapuran, Central Java Haryono, Sevien Elamaria; Aditiyarini, Dwi; Restiani, Ratih
Biogenesis: Jurnal Ilmiah Biologi Vol 12 No 1 (2024)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v12i1.40323

Abstract

Dendrophthoe pentandra (L.) Miq. are known to have the potential for health because they can be used as natural medicines to cure several diseases, including measles, coughs, diuretics, pain relievers, anti-inflammatory medications, as well as wound healing and infections. The local community in Indonesia has believed the health beneficial of tea mistletoe, but the information of its pharmacological activity is still limited. This research was conducted to explore the species and diversity of secondary metabolites of D. pentandra (L.) Miq. found in Sapuran, Wonosobo and its antioxidant activity. Extraction was performed by maceration in 96% ethanol. It is followed by phytochemical screening, determination of total secondary metabolite compounds, and antioxidant activity assay using DPPH. The results of this study confirmed that mistletoe is D. pentandra (L.). Its leaf extract has a yield of 8.76% that contains alkaloids, saponins, tannins, steroids, phenols, and terpenoids. Its total content of flavonoids, tannins, phenols and saponins was, respectively, 48.584 mg QE/g extract, 1.350 ± 0.140 mg TAE/g extract, 1.756 ± 0.171 g GAE/g extract, 4.665 ± 0.142 g SE/g extract. Its ethanolic extract has very high antioxidant activity with an IC50 value of 49.10 ppm. D. pentandra (L.) Miq. of Sapuran, Wonosobo, has great potential to be developed in the pharmacological field.
The Effect of Callus Age on Biomass and Flavonoid Production in Talinum paniculatum In Vitro Culture Restiani, Ratih; Aditiyarini, Dwi; Dolonseda, Anggel Christia; Nathania, Yohana Elsa
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 10, No 1: Jurnal Pembelajaran Dan Biologi Nukleus March 2024
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v10i1.5417

Abstract

Talinum paniculatum (Javanese Ginseng) is an herbaceous plant belonging to Talinaceae family that has been known as an important medicinal plant. Flavonoid is one of the prominent bioactive compounds that are contained in all parts of T. paniculatum and has been known for its several bioactivities, including antioxidant, anti-inflammatory, antimicrobial, antidiabetic, and anti-cancer. Callus culture can be used to ensure the quantity and quality of flavonoid production. Callus age is one of the important factors that needs to be considered in order to produce optimum biomass and flavonoid production. Therefore, this research aimed to investigate the effect of callus age on biomass and flavonoid production in T. panicultaum in vitro culture. Young leaves were used as an explant, and callus induction was performed using MS solid media supplemented with kinetin 3 mg/L and 2.4-D 2 mg/L. Biomass and flavonoid production were observed during 0, 28, 35, 42, and 49 days. Results showed that biomass and flavonoid production were influenced by callus age. The production of flavonoid in T. paniculatum callus culture was not linear with the growth of callus (biomass), and the optimum flavonoid production resulted in the 28th days of callus age (49.100 mg QE/g), while the optimum biomass resulted in the 49th days of callus age (4.295 g). It can be concluded that the optimum conditions for biomass and flavonoid production in T. paniculatum callus culture are influenced by different callus ages
PENGAJARAN DAN PRAKTIK UJI POTENSI PREBIOTIK PANGAN FUNGSIONAL LOKAL DI SMA NEGERI 7 YOGYAKARTA Ariestanti, Catarina; Nugroho, Wahyu Setyo; Kristiawan, Yoga Angkawijaya; Purba, Laurentia Henrieta Permita Sari; Restiani, Ratih
Servirisma Vol. 3 No. 2 (2023): Servirisma : Jurnal Pengabdian kepada Masyarakat
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat (LPPM) Universitas Kristen Duta Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21460/servirisma.2023.32.59

Abstract

Application of Biotechnology in Indonesia has been found in many sectors especially in food sector. One of the benefits is that it can be used to increase the nutritional value of food product so that it can be beneficial for health and decrease the risk of disease. The introduction of Biotechnology in utilizing local food product will increase students’ sensitivity about natural materials and their utilization. The purpose of this program was to introduce the role of Biotechnology in utilizing and processing agriculture by-product into beneficial edible end-product. This activity was divided into three stages: preparation, implementation, and reporting the activity. Preparation was done by contacting 7 State Senior High School as a partner. Implementation was conducted in partner’s Laboratory of Biology by delivering the teaching materials, continued with producing banana corm cookies and checked its prebiotik capability. Banana corm cookies were successfully produced. In vitro study showed that Lactobacillus plantarum FNCC-0265 were able to grow in banana corm cookies substituted media. The students showed a positive response during the activity, and it was shown by creation of the cookies and their ability to use drop plate technique for bacterial enumeration in vitro.
Cadmium Accumulation and Tolerance of Talinum paniculatum Callus Culture Miranda Gardha Viorenta; Ratih Restiani; Dwi Aditiyarini
Jurnal Biodjati Vol 10 No 1 (2025): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v10i1.38255

Abstract

The increasing use of inorganic fertilizers is one of the main factors contributing to increased Cadmium (Cd) pollution in the environment. Phytoremediation is one of the strategies that can be used to address the problem of Cd pollution in the environment. The selection of Cadmium-tolerant plants can be conducted using an in vitro culture. Talinum paniculatum as an ornamental plant is potentially used as a phytoremediation agent, but limited information is available regarding its accumulation ability and tolerance to cadmium stress. Therefore, this study aims to determine the accumulation ability and tolerance of T. paniculatum callus to cadmium at various concentrations. This study used a completely randomized design (CRD) with six replications of Cd concentration treatment (0, 5, 10, and 20 ppm). Accumulation and tolerance of callus to Cd were assessed based on callus biomass, callus color, tolerance index, heavy metal concentration in callus and media, and Bioconcentration Factor value. An increase in Cd concentration showed a change in callus color from yellowish green to blackish. In addition, there was no significant difference in dry weight (0.078-0.086 g) and tolerance index (102.631%-113.158%) of callus. However, increasing Cd concentration showed significant differences in callus Cd accumulation from media (5-20 ppm) and Bioconcentration Factor (BCF) ratio (1.282-5.701), indicating the ability of T. paniculatum as an accumulator plant. This study's results support phytoremediation efforts against heavy metal pollution, including cadmium.
Co-Authors Adityarini, Dwi Agustinus, Vania Alba Wijaya Maharani Anggel Christia Dolonseda Aniek Prasetyaningsih Aniek Prasetyaningsih Ariestanti, Catarina Astrid Helena Ayu Sekar, Astrid Christy, Nita Elvira Cindy Talenta Hutabarat Dena, Amelia Devi Sonti Sibarani Dolonseda, Anggel Christia Dwi Aditiyarini Dwi Adityarini Evieyana Evieyana Galgani, Gemma Gardha Viorenta, Miranda Haryono, Sevien Elamaria Heralius Dwiki Anggoro Josiah Herald Matheos Kaban, Sarah M.P. Kaban, Sarah Mega Pratenna Kendek Marendeng, Yunita Kristiawan, Yoga Angkawijaya Marendeng, Yunita Kendek Matheos, Josiah H. Matheos, Josiah Harold Matthew Linardi Meyga Semarayani, Cokorda Istri Miranda Gardha Viorenta Nathania, Yohana Elsa Nugroho, Wahyu Setyo Pono, Putri Purba, Laurentia Henrieta Permita Sari Putri Octaviana Ginting, Naomi Bastanta Rizki Wijaya Rolas Sinambela, Gracia Sekar, Astrid A. Sekar, Astrid Ayu Sibarani, Devi Sonti Sinaga, Meisi Olivia Viorenta, Miranda Gardha