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All Journal METAMORFOSA Journal of Biological Sciences Biotika: Jurnal Ilmiah Biologi Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Biogenesis: Jurnal Ilmiah Biologi Prosiding Seminar Biologi Edumatsains Jurnal Biodjati Jurnal Biologi Tropis Biosaintifika: Journal of Biology & Biology Education Jurnal Pembelajaran dan Biologi Nukleus Jurnal Pembelajaran Pemberdayaan Masyarakat (JP2M) Sciscitatio : Journal for Biological Science Jurnal Pengabdian kepada Masyarakat Nusantara JURNAL BIOLOGI PAPUA SERVIRISMA: Jurnal Pengabdian kepada Masyarakat Pelita: Jurnal Pengabdian kepada Masyarakat
Ratih Restiani
Program Studi Biologi, Fakultas Bioteknologi, Universitas Kristen Duta Wacana
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Mathematics Physics Social Sciences Other

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Kultur jaringan tumbuhan dasar sebagai upaya peningkatan pengetahuan dan keterampilan siswa Restiani, Ratih; Kaban, Sarah Mega Pratenna; Sekar, Astrid Ayu; Matheos, Josiah Harold; Galgani, Gemma
Jurnal Pembelajaran Pemberdayaan Masyarakat (JP2M) Vol. 5 No. 2 (2024)
Publisher : Universitas Islam Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33474/jp2m.v5i2.21800

Abstract

Perkembangan IPTEK saat ini berkontribusi dalam meningkatnya implementasi bioteknologi dalam kehidupan manusia. Kultur jaringan tumbuhan merupakan salah satu aplikasi bioteknologi di bidang pertanian. Pengenalan kultur jaringan tumbuhan sejak dini bagi siswa SMA menjadi salah satu upaya mempersiapkan SDM yang terampil. Namun demikian, siswa SMA masih mengalami kesulitan dalam proses pembelajaran bioteknologi terutama pada topik ini. Pembelajaran yang berfokus pada teori dan tidak dilengkapi dengan kegiatan praktikum merupakan penyebab siswa sulit memahami pelajaran tersebut. Kendala ini terutama dialami oleh siswa kelas XII SMA BOPKRI I Yogyakarta. Oleh karena itu, kegiatan pengabdian ini bertujuan untuk meningkatkan pengetahuan dan keterampilan dasar siswa pada materi kultur jaringan tumbuhan. Metode kegiatan pengabdian adalah participatory Action Research (PAR) yang diberikan dalam bentuk pelatihan. Pelaksanaan pelatihan dibagi menjadi dua sesi yaitu pemaparan materi pengantar, demonstrasi praktikum dan kegiatan praktikum. Acara praktikum meliputi pembuatan media kultur dan sterilisasi serta inokulasi eksplan. Berdasarkan hasil evaluasi, kegiatan pengabdian ini telah berhasil sebesar 100% dalam meningkatkan pengetahuan berdasarkan hasil tanya jawab selama pelatihan serta keterampilan dalam melaksanakan tahapan kultur jaringan tumbuhan. Kegiatan pengabdian kepada masyarakat ini lebih lanjut dapat diintegrasikan dengan kegiatan pembelajaran biologi di kelas sehingga proses pelatihan dan pendampingan mengenai kultur jaringan tumbuhan dapat berlangsung lebih intensif. Selain itu, project kelompok yang diberikan selama kegiatan pengabdian dapat membantu siswa dalam mencapai tujuan pembelajaran bioteknologi di kelas.
Effect of Citric Acid and Polyvinylpyrrolidone (PVP) on Browning of Kepel (Stelechocarpus burahol (Blume) Hook.f. & Thomson) Nodes Marendeng, Yunita Kendek; Viorenta, Miranda Gardha; Sinaga, Meisi Olivia; Christy, Nita Elvira; Sibarani, Devi Sonti; Restiani, Ratih
Jurnal Biologi Tropis Vol. 24 No. 4 (2024): Oktober - Desember
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i4.7647

Abstract

Browning is a major constraint in in vitro propagation of Kepel (S. burahol). Therefore, the selection of an effective anti-browning compound can be useful to prevent browning in Kepel in vitro culture and produce explants that are able to regenerate. This study was aimed to investigate the effect of the addition of citric acid and PVP at different concentrations and incubation conditions on the prevention of browning in in vitro cultures of S. burahol. This research is an experimental study using S.burahol node explants. The research design used a Completely Randomized Design (CRD) with citric acid and PVP treatments of 300 and 400 ppm respectively and control incubated in dark and light conditions. Each treatment was repeated twice. The culture was incubated for 28 days. Observations were conducted on browning initiation time, browning intensity and callus initiation time. Data were analyzed descriptively. The results showed that citric acid treatment of 300 and 400 ppm and incubation of cultures in dark conditions effectively reduced the intensity of browning (0.2) and browning initiation time (6 DAI). In addition, the suplementation of 400 ppm citric acid and incubation in light conditions was also effective in initiating the fastest callus at 2 DAI. The results suggest that the addition of citric acid at concentrations of 300 and 400 ppm is more effective at slowing browning and reducing browning intensity for S. burahol node explants than PVP. The results of this study play an important role in supporting efforts to increase the productivity of S. burahol nodes growth through in vitro culture.
Edukasi Kultur Jaringan Tumbuhan bagi Siswa SMA Stella Duce 2 Yogyakarta Restiani, Ratih; Galgani, Gemma; Ayu Sekar, Astrid; Kendek Marendeng, Yunita; Rolas Sinambela, Gracia; Gardha Viorenta, Miranda
Jurnal Pengabdian kepada Masyarakat Nusantara Vol. 5 No. 2 (2024): Jurnal Pengabdian kepada Masyarakat Nusantara (JPkMN)
Publisher : Lembaga Dongan Dosen

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55338/jpkmn.v5i2.3211

Abstract

Kultur jaringan tumbuhan merupakan bagian dari topik bioteknologi yang dipelajari siswa SMA pada mata pelajaran Biologi di Sekolah Menengah Atas. Meskipun demikian, dalam prosesnya siswa mengalami kesulitan dalam pembelajaran bioteknologi terutama kultur jaringan tumbuhan karena pembelajaran berfokus pada pemberian teori tanpa dilengkapi dengan kegiatan praktikum. Selain itu, fasilitas laboratorium yang belum memadai menjadi faktor pembatas bagi pelaksanaan praktikum ini. Kesulitan dalam pembelajaran kultur jaringan tumbuhan ini juga dialami oleh siswa kelas X di SMA Stella Duce 2 Yogyakarta. Dalam upaya mendukung capaian pembelajaran topik Bioteknologi di kelas X dan mengatasi kesulitan dalam proses pembelajaran, maka kegiatan pengabdian dalam bentuk edukasi perlu dilakukan. Kegiatan pengabdian kepada masyarakat ini bertujuan untuk meningkatkan pengetahuan dan keterampilan melalui pengalaman praktikum pada topik kultur jaringan tumbuhan. Tahap pelaksanaan kegiatan meliputi tahap persiapan, pelaksanaan kegiatan (pemberian materi dan demonstrasi) dan tahap evaluasi. Hasil yang diperoleh selama kegiatan berlangsung adalah siswa terlibat secara aktif dalam proses diskusi dan tanya jawab, sebagian besar siswa dapat menjawab pertanyaan selama diskusi dan semua siswa berpartisipasi aktif dalam kegiatan praktikum serta mampu menyelesaikan kegiatan praktikum sesuai waktu yang diberikan. Berdasarkan hasil pelaksanaan tersebut, dapat disimpulkan bahwa kegiatan pengabdian berupa edukasi ini dapat membantu siswa dalam meningkatkan pengetahuan dan keterampilan dalam pembelajaran kultur jaringan tumbuhan.
Respon Pertumbuhan dan Toleransi Kalus Talinum paniculatum terhadap Cekaman Logam Berat Krom (Cr) Ratih Restiani; Kaban, Sarah M.P.; Sekar, Astrid A.; Matheos, Josiah H.
Jurnal Biologi Papua Vol 16 No 1 (2024)
Publisher : Jurusan Biologi FMIPA Universitas Cenderawasih

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31957/jbp.3590

Abstract

The escalating chromium (Cr) heavy metal contamination necessitates effective remediation strategies like phytoremediation. Selecting chromium-tolerant plant species is crucial for successful phytoremediation, achievable through in vitro culture to induce somaclonal variations with resistance traits. Talinum paniculatum, an ornamental plant with robust roots and stress resilience, is a promising candidate for chromium tolerance studies. This research assesses T. paniculatum callus response to chromium stress, focusing on growth and tolerance indices. Results demonstrate the callus's resilience to increasing Cr concentrations, reflected in biomass increase (0.117–0.150 g) and a high tolerance index (TI) of 77.78–100%. Structural integrity and color remained stable throughout the study period, underscoring T. paniculatum's potential for chromium phytoremediation. 
Effect of Plant Growth Regulators (PGRs) on Biomass and Flavonoid Production of Talinum paniculatum Callus Culture Kaban, Sarah Mega Pratenna; Restiani, Ratih; Aditiyarini, Dwi
Jurnal Biodjati Vol 9 No 1 (2024): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v9i1.33788

Abstract

Talinum paniculatum Jacq (Gaertn), locally known as Javanese Ginseng, is an essential medicinal plant characterized by high flavonoid content with antioxidant and antimicrobial activities. Compared to other natural sources, a larger number of T. paniculatum leaves is required to obtain high amounts of flavonoid for drug development. In this context, in vitro culture can be used to increase flavonoid production in a controlled condition without reducing plant population. The concentration of Plant Growth Regulators (PGRs) is capable of influencing biomass and flavonoid accumulation in callus culture from several species. Despite these benefits, there is limited information about the optimal concentration of PGRs for flavonoid production from T. paniculatum callus culture. Therefore, this study aimed to investigate the optimum concentration of 2,4-D and kinetin for callus growth, as well as flavonoid production from T. paniculatum. The investigation was carried out using a completely randomized design (CRD), where young leaves explants were cultured on MS media supplemented with various concentrations of 2,4-D (0, 0.5, 1, 2) mg/L and kinetin (0, 0.5, 1, 2) mg/L for callus and flavonoid production. The results showed that the highest callus biomass of 0.105 g was produced from MS medium with 2 mg/L of 2,4-D and 1 mg/L of kinetin. Meanwhile, the highest total flavonoid content of 25.66 mg QE/g DW was produced from media supplemented with 1 mg/Lof 2,4-D and 2 mg/L of kinetin. These results showed that different combinations of 2,4-D and kinetin were required to increase biomass and flavonoid production. In conclusion, this study provided valuable information for the development of bioactive compounds through in vitro culture.
Pengaruh Konsentrasi Air Kelapa dan BAP (6-Benzylaminopurin) terhadap Regenerasi In vitro Protokorm Dendrobium stratiotes x Dendrobium wulaiense Agustinus, Vania; Restiani, Ratih; Prasetyaningsih, Aniek
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 9, No 2 (2024): June 2024
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v9i2.7728

Abstract

Kultur in vitro merupakan salah satu solusi alternatif untuk mengatasi kesulitan perbanyakan anggrek melalui biji. Keberhasilan regenerasi protokorm anggrek dipengaruhi oleh penambahan ZPT dan air kelapa ke dalam medium. Oleh karena itu, penelitian ini bertujuan untuk menentukan pengaruh konsentrasi air kelapa dan BAP terhadap regenerasi protokorm Dendrobium hibrida. Penelitian menggunakan Rancangan Acak Lengkap dengan dua faktor yaitu konsentrasi air kelapa dan BAP. Paramater yang diamati meliputi persentase tunas, jumlah tunas, tinggi protokorm, dan persentase akar. Hasil menunjukkan bahwa penambahan air kelapa dan BAP ke dalam medium VW berpengaruh nyata terhadap tinggi dan persentase akar protokorm. Konsentrasi air kelapa (10%) tanpa penambahan BAP dapat meningkatkan persentase tunas, akar, jumlah tunas, dan tinggi protokorm Dendrobium hibrida. Hasil penelitian menunjukan bahwa penggunaan air kelapa secara tunggal maupun kombinasi dengan hormon sitokinin (BAP) signifikan dalam mempercepat regenerasi protokorm Dendrobium hibrida. Ini merupakan penelitian pertama yang melaporkan penggunaan air kelapa dan BAP pada regenerasi protokorm hasil persilangan Dendrobium stratiotes x Dendrobium wulaiense.
Sosialisasi Budidaya Microgreens Skala Rumah Tangga bagi Masyarakat di Kelurahan Pandeyan Yogyakarta Restiani, Ratih
Pelita: Jurnal Pengabdian kepada Masyarakat Vol. 2 No. 4 (2022): Pelita: Jurnal Pengabdian kepada Masyarakat
Publisher : Perkumpulan Kualitama Edukatika Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (335.56 KB) | DOI: 10.51651/pjpm.v2i4.318

Abstract

Microgreens have emerged as a new trend in urban farming due to more practice, efficiency, affordable, short periods of cultivation, and highly nutrition than other types of urban farming methods. The increase of physical development in Pandeyan, ??Yogyakarta due to the increase in population has caused a decrease in the area for plant cultivation. One of the solutions to overcome this problem is through the socialization of household-scale microgreens cultivation. Therefore, this socialization aimed to enhance the knowledge of the Pandeyan community regarding the definition of microgreens, types of plants that can be cultivated, benefits, stages of cultivation, and business opportunities. The socialization was delivered in the form of a webinar on Tuesday, 30th November 2021 using Zoom platform, with a total 32 of participants. This activity is divided into 3 stages: introduction, transfer of knowledge, and discussion. Based on the evaluation, this activity has succeeded in increasing the community's knowledge about microgreens, their benefits, and the stages of microgreens cultivation based on the high enthusiasm of the community during the discussion session.
The Effect of IAA, BAP, and Coconut Water on Tomato (Solanum lycopersicum L.) Organogenesis Alba Wijaya Maharani; Restiani, Ratih; Aniek Prasetyaningsih
Jurnal Biodjati Vol 10 No 2 (2025): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v10i2.49410

Abstract

Tomato (Solanum lycopersicum L.) is a widely cultivated horticultural crop valued for its phytochemical and nutritional content. The increasing demand for high-quality seedlings has promoted the use of in vitro propagation, which offers greater efficiency in labor and land use, uniform seedling production, and independence from climate compared to conventional methods. This study aimed to investigate the effect of different concentrations of plant growth regulators (PGRs) and coconut water as an alternative or supplement on the organogenesis of tomato hypocotyl explants. Tomato hypocotyl explants were cultured on Murashige and Skoog (MS) medium supplemented with different concentrations of BAP (0.5, 1.0, and 1.5 ppm), IAA (0.1, 0.3, and 0.5 ppm), and coconut water (10%, 20%, and 30%). The experiment was arranged in a Completely Randomized Design (CRD) with four replications, consisting of a control, single-factor treatments, and combination treatments of BAP, IAA, and coconut water. Observations included the percentage and intensity of root, shoot, and callus formation, as well as shoot height. Data were analyzed using the non-parametric Kruskal–Wallis test, followed by Dunn’s test. The results showed that roots, shoots, and callus successfully developed in treatments supplemented with 6-benzylaminopurine (BAP), indole-3-acetic acid (IAA), and coconut water. The highest shoot growth, with a percentage of 87.7% and an average height of 5.75 cm, was observed in treatment K20, while the highest callus formation occurred in treatment K30. The best shoot and callus intensities were resulted in media with coconut water alone at concentrations of 10%, 20%, and 30%, whereas the highest root intensity was obtained in the BAP+IAA treatment without coconut water. The results indicate that coconut water is effective in supporting organogenesis, both directly through the formation of shoots and roots, and indirectly through callus formation that can subsequently develop into shoots and roots.
Integrasi Laboratorium Mini Kultur Jaringan Tumbuhan dalam Pembelajaran Bioteknologi Siswa SMA BOPKRI 2 Yogyakarta Restiani, Ratih; Galgani, Gemma; Sekar, Astrid Ayu; Marendeng, Yunita Kendek; Sinambela, Gracia Rolas; Viorenta, Miranda Gardha
Jurnal Pengabdian kepada Masyarakat Nusantara Vol. 7 No. 1 (2026): Edisi Januari - Maret
Publisher : Lembaga Dongan Dosen

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55338/jpkmn.v7i1.8444

Abstract

Pembelajaran kultur jaringan tumbuhan pada mata pelajaran Biologi di SMA BOPKRI 2 Yogyakarta masih didominasi oleh pemberian teori di kelas karena keterbatasan fasilitas laboratorium, sehingga siswa belum memiliki pengalaman praktik bioteknologi. Kondisi ini menyebabkan rendahnya pemahaman aplikatif dan keterampilan bioteknologi siswa. Program ini menawarkan solusi berupa pelatihan kultur jaringan berbasis laboratorium mini yang terintegrasi dengan pembelajaran Biologi bagi siswa kelas X. Kegiatan dilaksanakan melalui tahapan koordinasi dengan guru mitra, penyusunan modul dan persiapan alat dan bahan praktikum, pelaksanaan pelatihan (pemberian teori dan praktikum) selama dua hari, serta evaluasi berbasis observasi keterampilan dan pengetahuan siswa. Pelatihan melibatkan 71 siswa dari tiga kelas X melalui penyampaian materi pengantar, demonstrasi pembuatan medium, sterilisasi dan inokulasi eksplan, dilaknjutkan praktikum membuat media kultur dan inokulasi eksplan secara berkelompok. Hasil evaluasi menunjukkan peningkatan signifikan dalam keterampilan dan pengetahuan siswa. Sebelum pelatihan, siswa belum mengenal definisi, manfaat dan tahapan kerja kultur jaringan tumbuhan. Setelah pelatihan, hasil evaluasi menunjukkan peningkatan keterampilan dan pengetahuan siswa dalam mempelajari aplikasi bioteknologi. Indikator keberhasilan pelatihan ini adalah peningkatan keterampilan siswa dari 0% menjadi 100% mampu dalam membuat media kultur jaringan dasar yaitu Murashige and Skoog (MS) dan menginokulasi eksplan steril ke dalam medium MS sesuai dengan prosedur dan waktu yang diberikan. Selain peningkatan dalam keterampilan, pelatihan ini juga meningkatkan pemahaman pengetahuan siswa (dari 0% menjadi 100%) dalam menjelaskan manfaat, tujuan dan tahapan pembuatan media MS dan inokulasi eksplan dengan tepat. Berdasarkan dua indikator tersebut, maka dapat disimpulkan bahwa pelaksanaan mini laboratorium kultur jaringan tumbuhan yang diintegrasikan dalam pembelajaran bioteknologi dasar bagi siswa mampu membantu siswa dalam meningkatkan pengetahuan dan keterampilan di mata pelajaran Bioteknologi.
Effect of BAP and 2,4-D Combination on the Callus Induction of Robusta Coffee (Coffea canephora Pierre ex A.Froehner) Leaf Explants Wijaya, Yovita Angeline; Restiani, Ratih; Prasetyaningsih, Aniek
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 11, No 4: Jurnal Pembelajaran Dan Biologi Nukleus December 2025
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v11i4.8338

Abstract

Background: Sustainable production of Robusta coffee (Coffea canephora) is constrained by the limited availability of high-quality planting materials, while information regarding the optimal auxin–cytokinin combinations for initiating callus cultures remains insufficient. Accordingly, this study was conducted to examine the effects of combined applications of benzyl amino purine (BAP) and 2,4-dichlorophenoxyacetic acid (2,4-D) on callus induction and subsequent growth derived from young leaf explants of Robusta coffee. Methodology: Explant cultures were established on Murashige and Skoog (MS) medium enriched with different concentrations of 2,4-D (1, 1.5, and 2 ppm) in combination with BAP (1.5, 2, and 2.5 ppm), arranged in a completely randomized design with three replications. Data collection was conducted throughout a 30-day incubation period and included measurements of callus initiation time (days), callus formation percentage, callus growth intensity, and callus morphological features. The resulting data were analyzed statistically using analysis of variance (ANOVA), followed by the Kruskal–Wallis test. Findings: Result describe that the treatment combining 2,4-D at 1 ppm with BAP at 1.5 ppm produced the most favorable response, as indicated by the shortest callus initiation period (7.33 DAP). In contrast, the application of 2,4-D at 1 ppm in combination with BAP at 2.5 ppm achieved a 100% callus induction rate and the highest callus growth intensity (2 on a 5-point scale), along with a compact callus exhibiting a yellowish-green coloration. Overall, these results highlight the critical influence of auxin–cytokinin equilibrium in enhancing callus induction in Robusta coffee and underscore its significance as a foundational reference for establishing an efficient in vitro propagation strategy. Contribution: In general, this study contributes to providing important technical information supporting the large-scale and sustainable production of high-quality Robusta coffee seedlings
Co-Authors Adityarini, Dwi Agustinus, Vania Alba Wijaya Maharani Anggel Christia Dolonseda Aniek Prasetyaningsih Aniek Prasetyaningsih Ariestanti, Catarina Astrid Helena Ayu Sekar, Astrid Christy, Nita Elvira Cindy Talenta Hutabarat Dena, Amelia Devi Sonti Sibarani Dolonseda, Anggel Christia Dwi Aditiyarini Dwi Adityarini Evieyana Evieyana Galgani, Gemma Gardha Viorenta, Miranda Haryono, Sevien Elamaria Heralius Dwiki Anggoro Josiah Herald Matheos Kaban, Sarah M.P. Kaban, Sarah Mega Pratenna Kendek Marendeng, Yunita Kristiawan, Yoga Angkawijaya Marendeng, Yunita Kendek Matheos, Josiah H. Matheos, Josiah Harold Matthew Linardi Meyga Semarayani, Cokorda Istri Miranda Gardha Viorenta Nathania, Yohana Elsa Nugroho, Wahyu Setyo Pono, Putri Purba, Laurentia Henrieta Permita Sari Putri Octaviana Ginting, Naomi Bastanta Rizki Wijaya Rolas Sinambela, Gracia Sekar, Astrid A. Sekar, Astrid Ayu Sibarani, Devi Sonti Sinaga, Meisi Olivia Sinambela, Gracia Rolas Viorenta, Miranda Gardha Wijaya, Yovita Angeline