Article Per Year (5 Year)
p-Index From 2021 - 2026
3.872
P-Index
This Author published in this journals
All Journal Biotika: Jurnal Ilmiah Biologi Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Biogenesis: Jurnal Ilmiah Biologi Prosiding Seminar Biologi Biology, Medicine, & Natural Product Chemistry Edumatsains Jurnal Biodjati Jurnal Biologi Tropis Biosaintifika: Journal of Biology & Biology Education Jurnal Pembelajaran dan Biologi Nukleus Jurnal Pembelajaran Pemberdayaan Masyarakat (JP2M) Sciscitatio : Journal for Biological Science Jurnal Pengabdian kepada Masyarakat Nusantara JURNAL BIOLOGI PAPUA Pelita: Jurnal Pengabdian kepada Masyarakat
Ratih Restiani
Program Studi Biologi, Fakultas Bioteknologi, Universitas Kristen Duta Wacana
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Public Health Social Sciences Other

Published : 27 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 27 Documents
Search

 1   2   3 
Respon Pertumbuhan dan Toleransi Kalus Talinum paniculatum terhadap Cekaman Logam Berat Krom (Cr) Ratih Restiani; Kaban, Sarah M.P.; Sekar, Astrid A.; Matheos, Josiah H.
Jurnal Biologi Papua Vol 16 No 1 (2024)
Publisher : Jurusan Biologi FMIPA Universitas Cenderawasih

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31957/jbp.3590

Abstract

The escalating chromium (Cr) heavy metal contamination necessitates effective remediation strategies like phytoremediation. Selecting chromium-tolerant plant species is crucial for successful phytoremediation, achievable through in vitro culture to induce somaclonal variations with resistance traits. Talinum paniculatum, an ornamental plant with robust roots and stress resilience, is a promising candidate for chromium tolerance studies. This research assesses T. paniculatum callus response to chromium stress, focusing on growth and tolerance indices. Results demonstrate the callus's resilience to increasing Cr concentrations, reflected in biomass increase (0.117–0.150 g) and a high tolerance index (TI) of 77.78–100%. Structural integrity and color remained stable throughout the study period, underscoring T. paniculatum's potential for chromium phytoremediation. 
Pengaruh Konsentrasi Air Kelapa dan BAP (6-Benzylaminopurin) terhadap Regenerasi In vitro Protokorm Dendrobium stratiotes x Dendrobium wulaiense Agustinus, Vania; Restiani, Ratih; Prasetyaningsih, Aniek
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 9, No 2 (2024): June 2024
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v9i2.7728

Abstract

Kultur in vitro merupakan salah satu solusi alternatif untuk mengatasi kesulitan perbanyakan anggrek melalui biji. Keberhasilan regenerasi protokorm anggrek dipengaruhi oleh penambahan ZPT dan air kelapa ke dalam medium. Oleh karena itu, penelitian ini bertujuan untuk menentukan pengaruh konsentrasi air kelapa dan BAP terhadap regenerasi protokorm Dendrobium hibrida. Penelitian menggunakan Rancangan Acak Lengkap dengan dua faktor yaitu konsentrasi air kelapa dan BAP. Paramater yang diamati meliputi persentase tunas, jumlah tunas, tinggi protokorm, dan persentase akar. Hasil menunjukkan bahwa penambahan air kelapa dan BAP ke dalam medium VW berpengaruh nyata terhadap tinggi dan persentase akar protokorm. Konsentrasi air kelapa (10%) tanpa penambahan BAP dapat meningkatkan persentase tunas, akar, jumlah tunas, dan tinggi protokorm Dendrobium hibrida. Hasil penelitian menunjukan bahwa penggunaan air kelapa secara tunggal maupun kombinasi dengan hormon sitokinin (BAP) signifikan dalam mempercepat regenerasi protokorm Dendrobium hibrida. Ini merupakan penelitian pertama yang melaporkan penggunaan air kelapa dan BAP pada regenerasi protokorm hasil persilangan Dendrobium stratiotes x Dendrobium wulaiense.
Sosialisasi Budidaya Microgreens Skala Rumah Tangga bagi Masyarakat di Kelurahan Pandeyan Yogyakarta Restiani, Ratih
Pelita: Jurnal Pengabdian kepada Masyarakat Vol. 2 No. 4 (2022): Pelita: Jurnal Pengabdian kepada Masyarakat
Publisher : Perkumpulan Kualitama Edukatika Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (335.56 KB) | DOI: 10.51651/pjpm.v2i4.318

Abstract

Microgreens have emerged as a new trend in urban farming due to more practice, efficiency, affordable, short periods of cultivation, and highly nutrition than other types of urban farming methods. The increase of physical development in Pandeyan, ??Yogyakarta due to the increase in population has caused a decrease in the area for plant cultivation. One of the solutions to overcome this problem is through the socialization of household-scale microgreens cultivation. Therefore, this socialization aimed to enhance the knowledge of the Pandeyan community regarding the definition of microgreens, types of plants that can be cultivated, benefits, stages of cultivation, and business opportunities. The socialization was delivered in the form of a webinar on Tuesday, 30th November 2021 using Zoom platform, with a total 32 of participants. This activity is divided into 3 stages: introduction, transfer of knowledge, and discussion. Based on the evaluation, this activity has succeeded in increasing the community's knowledge about microgreens, their benefits, and the stages of microgreens cultivation based on the high enthusiasm of the community during the discussion session.
Integrasi Laboratorium Mini Kultur Jaringan Tumbuhan dalam Pembelajaran Bioteknologi Siswa SMA BOPKRI 2 Yogyakarta Restiani, Ratih; Galgani, Gemma; Sekar, Astrid Ayu; Marendeng, Yunita Kendek; Sinambela, Gracia Rolas; Viorenta, Miranda Gardha
Jurnal Pengabdian kepada Masyarakat Nusantara Vol. 7 No. 1 (2026): Edisi Januari - April
Publisher : Lembaga Dongan Dosen

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55338/jpkmn.v7i1.8444

Abstract

Pembelajaran kultur jaringan tumbuhan pada mata pelajaran Biologi di SMA BOPKRI 2 Yogyakarta masih didominasi oleh pemberian teori di kelas karena keterbatasan fasilitas laboratorium, sehingga siswa belum memiliki pengalaman praktik bioteknologi. Kondisi ini menyebabkan rendahnya pemahaman aplikatif dan keterampilan bioteknologi siswa. Program ini menawarkan solusi berupa pelatihan kultur jaringan berbasis laboratorium mini yang terintegrasi dengan pembelajaran Biologi bagi siswa kelas X. Kegiatan dilaksanakan melalui tahapan koordinasi dengan guru mitra, penyusunan modul dan persiapan alat dan bahan praktikum, pelaksanaan pelatihan (pemberian teori dan praktikum) selama dua hari, serta evaluasi berbasis observasi keterampilan dan pengetahuan siswa. Pelatihan melibatkan 71 siswa dari tiga kelas X melalui penyampaian materi pengantar, demonstrasi pembuatan medium, sterilisasi dan inokulasi eksplan, dilaknjutkan praktikum membuat media kultur dan inokulasi eksplan secara berkelompok. Hasil evaluasi menunjukkan peningkatan signifikan dalam keterampilan dan pengetahuan siswa. Sebelum pelatihan, siswa belum mengenal definisi, manfaat dan tahapan kerja kultur jaringan tumbuhan. Setelah pelatihan, hasil evaluasi menunjukkan peningkatan keterampilan dan pengetahuan siswa dalam mempelajari aplikasi bioteknologi. Indikator keberhasilan pelatihan ini adalah peningkatan keterampilan siswa dari 0% menjadi 100% mampu dalam membuat media kultur jaringan dasar yaitu Murashige and Skoog (MS) dan menginokulasi eksplan steril ke dalam medium MS sesuai dengan prosedur dan waktu yang diberikan. Selain peningkatan dalam keterampilan, pelatihan ini juga meningkatkan pemahaman pengetahuan siswa (dari 0% menjadi 100%) dalam menjelaskan manfaat, tujuan dan tahapan pembuatan media MS dan inokulasi eksplan dengan tepat. Berdasarkan dua indikator tersebut, maka dapat disimpulkan bahwa pelaksanaan mini laboratorium kultur jaringan tumbuhan yang diintegrasikan dalam pembelajaran bioteknologi dasar bagi siswa mampu membantu siswa dalam meningkatkan pengetahuan dan keterampilan di mata pelajaran Bioteknologi.
Protective Effect of Ethanolic Mistletoe Leaves (Scurula atropurpurea (Bl.) Dans) Extract Against Protein Denaturation In Vitro Dwi Aditiyarini; Christini Bernaindah Nadapdap; Ratih Restiani
Biology, Medicine, & Natural Product Chemistry Vol 15, No 1 (2026)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2026.151.391-399

Abstract

Inflammation is a complex biological response that eliminates harmful stimuli, clears damaged tissues, and initiates healing. Protein denaturation is known to trigger inflammatory reactions and is associated with chronic disorders such as rheumatoid arthritis, lupus erythematosus, and serum sickness. Although steroidal and non-steroidal anti-inflammatory drugs (NSAIDs) are widely used, their long-term use may cause adverse effects. Herbal plants represent potential alternative therapies with fewer side effects. This study examined the anti-inflammatory potential of Scurrula atropurpurea, a hemiparasitic plant traditionally used for various medicinal purposes. The ethanolic leaf extract was evaluated for its phytochemical composition, antioxidant properties, and ability to inhibit egg albumin denaturation in vitro. Fresh leaves were collected from tea plants in Sapuran, Wonosobo, and extracted using Soxhlet with 96% ethanol. Phytochemical screening indicated the presence of alkaloids, flavonoids, tannins, phenolics, saponins, steroids, and terpenoids. Quantitative analysis showed a high total phenolic content (126.10 ± 1.5 mg GAE/g extract), moderate tannins, and relatively low flavonoids. The extract demonstrated weak antioxidant activity (IC50 = 140.4 ± 4.9 µg/mL) and minimal protein denaturation inhibition (maximum 1.51% at 100 ppm), significantly lower than sodium diclofenac as a positive control. Overall, the ethanolic extract of S. atropurpurea leaves exhibited low in vitro anti-inflammatory activity, suggesting the need for further investigation using alternative extraction methods, plant parts, or host species.
Effect of BAP and 2,4-D Combination on the Callus Induction of Robusta Coffee (Coffea canephora Pierre ex A.Froehner) Leaf Explants Yovita Angeline Wijaya; Ratih Restiani; Aniek Prasetyaningsih
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 11, No 4: Jurnal Pembelajaran Dan Biologi Nukleus December 2025
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v11i4.8338

Abstract

Background: Sustainable production of Robusta coffee (Coffea canephora) is constrained by the limited availability of high-quality planting materials, while information regarding the optimal auxin–cytokinin combinations for initiating callus cultures remains insufficient. Accordingly, this study was conducted to examine the effects of combined applications of benzyl amino purine (BAP) and 2,4-dichlorophenoxyacetic acid (2,4-D) on callus induction and subsequent growth derived from young leaf explants of Robusta coffee. Methodology: Explant cultures were established on Murashige and Skoog (MS) medium enriched with different concentrations of 2,4-D (1, 1.5, and 2 ppm) in combination with BAP (1.5, 2, and 2.5 ppm), arranged in a completely randomized design with three replications. Data collection was conducted throughout a 30-day incubation period and included measurements of callus initiation time (days), callus formation percentage, callus growth intensity, and callus morphological features. The resulting data were analyzed statistically using analysis of variance (ANOVA), followed by the Kruskal–Wallis test. Findings: Result describe that the treatment combining 2,4-D at 1 ppm with BAP at 1.5 ppm produced the most favorable response, as indicated by the shortest callus initiation period (7.33 DAP). In contrast, the application of 2,4-D at 1 ppm in combination with BAP at 2.5 ppm achieved a 100% callus induction rate and the highest callus growth intensity (2 on a 5-point scale), along with a compact callus exhibiting a yellowish-green coloration. Overall, these results highlight the critical influence of auxin–cytokinin equilibrium in enhancing callus induction in Robusta coffee and underscore its significance as a foundational reference for establishing an efficient in vitro propagation strategy. Contribution: In general, this study contributes to providing important technical information supporting the large-scale and sustainable production of high-quality Robusta coffee seedlings
Cadmium Accumulation and Tolerance of Talinum paniculatum Callus Culture Miranda Gardha Viorenta; Ratih Restiani; Dwi Aditiyarini
Jurnal Biodjati Vol 10 No 1 (2025): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v10i1.38255

Abstract

The increasing use of inorganic fertilizers is one of the main factors contributing to increased Cadmium (Cd) pollution in the environment. Phytoremediation is one of the strategies that can be used to address the problem of Cd pollution in the environment. The selection of Cadmium-tolerant plants can be conducted using an in vitro culture. Talinum paniculatum as an ornamental plant is potentially used as a phytoremediation agent, but limited information is available regarding its accumulation ability and tolerance to cadmium stress. Therefore, this study aims to determine the accumulation ability and tolerance of T. paniculatum callus to cadmium at various concentrations. This study used a completely randomized design (CRD) with six replications of Cd concentration treatment (0, 5, 10, and 20 ppm). Accumulation and tolerance of callus to Cd were assessed based on callus biomass, callus color, tolerance index, heavy metal concentration in callus and media, and Bioconcentration Factor value. An increase in Cd concentration showed a change in callus color from yellowish green to blackish. In addition, there was no significant difference in dry weight (0.078-0.086 g) and tolerance index (102.631%-113.158%) of callus. However, increasing Cd concentration showed significant differences in callus Cd accumulation from media (5-20 ppm) and Bioconcentration Factor (BCF) ratio (1.282-5.701), indicating the ability of T. paniculatum as an accumulator plant. This study's results support phytoremediation efforts against heavy metal pollution, including cadmium.
Co-Authors Adityarini, Dwi Agustinus, Vania Anggel Christia Dolonseda Aniek Prasetyaningsih Aniek Prasetyaningsih Aniek Prasetyaningsih Astrid Helena Ayu Sekar, Astrid Christini Bernaindah Nadapdap Christy, Nita Elvira Cindy Talenta Hutabarat Dena, Amelia Devi Sonti Sibarani Dolonseda, Anggel Christia Dwi Aditiyarini Dwi Adityarini Evieyana Evieyana Galgani, Gemma Gardha Viorenta, Miranda Haryono, Sevien Elamaria Heralius Dwiki Anggoro Josiah Herald Matheos Kaban, Sarah M.P. Kaban, Sarah Mega Pratenna Kendek Marendeng, Yunita Marendeng, Yunita Kendek Matheos, Josiah H. Matheos, Josiah Harold Matthew Linardi Meyga Semarayani, Cokorda Istri Miranda Gardha Viorenta Nathania, Yohana Elsa Pono, Putri Putri Octaviana Ginting, Naomi Bastanta Rizki Wijaya Rolas Sinambela, Gracia Sekar, Astrid A. Sekar, Astrid Ayu Sibarani, Devi Sonti Sinaga, Meisi Olivia Sinambela, Gracia Rolas Viorenta, Miranda Gardha Yovita Angeline Wijaya