Claim Missing Document
Check
Articles

Found 12 Documents
Search

Lysinibacillus sphaericus Isolated from Palm Oil Waste Land as Lipase Producer Sumarsih, Sri; Sofijan Hadi; Fatimah; Rizka Diah Fitri; Gilva Illavi
HAYATI Journal of Biosciences Vol. 31 No. 3 (2024): May 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.3.425-431

Abstract

In this research, lipolyic bacteria have been isolated from palm oil waste land for the production of lipase. Species of potential lipolytic bacteria were identified based on their morphology and sequences of 16 rRNA gene. Enzymes are produced by growing bacteria in a medium with various vegetable oils and nitrogen sources. The enzyme produced by the bacteria measured its lipolytic activity against the substrate para-nitrophenylpalmitate. The lipolytic bacteria was recognized as Lysinibacillus sphaericus L49a based on morphological and phylogenetic analysis. Mineral media with different vegetable oils as carbon sources, and different nitrogen sources were suitable for growth and production of lipase enzymes of L. sphaericus L49a. Culltivation of L. sphaericus L49a in medium containing ammonium sulfate and olive oil produced lipase with the highest lipolytic activity.
COMMUNITY DEVELOPMENT THROUGH EDUCATION ON THE PROCESSING OF HOUSEHOLD WASTE INTO COMMERCIAL PRODUCTS Widati, Alfa Akustia; Adiarto, Tokok; Zakki Fahmi, Mochamad; Budi Prasetyo, Antonius; Darmokoesoemo, Handoko; Supriyanto, Ganden; Sumarsih, Sri; Purwaningsih, Aning; Khasanah, Miratul; Hartati, Hartati; Baktir, Afaf; Hadi, Sofijan; Purkan, Purkan; Raharjo, Yanuardi; Khairunnisa, Fatiha; A'yuni, Qurrota; Tri Puspaningsih, Ni Nyoman; Hwei Voon, Lee
Jurnal Layanan Masyarakat (Journal of Public Services) Vol. 8 No. 3 (2024): JURNAL LAYANAN MASYARAKAT
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jlm.v8i3.2024.329-338

Abstract

Community empowerment and household waste processing are two issues that need to be resolved by the government. An independent and educated society is an important pillar to support the development of country. Meanwhile, waste that continues to accumulate without processing has a negative impact on health and the environment. Public education about household waste processing can be a solution to the two problems above. Education was carried out at Junior High School YBPK Surabaya and residents of Dukuh Kupang Surabaya, and other participants who joined in a hibrid meeting with a total of 116 participants. Activities at YBPK Surabaya took place on 25 March and 5 August 2023 and activities at Dukuh Kupang Surabaya took place on 27 May and 26 August 2023. The types of waste processed are organic waste and used cooking oil, which are then converted into eco-enzymes and used cooking oil Activities are divided into 2 stages. there are community education activities and program sustainability monitoring activities. The community education activities includes presentation and workshop were hold in March 2023 at YBPK Surabaya Middle School and May 2023 at Dukuh Kupang Surabaya. The evaluation of sustainability program including individual practice and monitoring of activities was carried out in August 2023 at Dukuh Kupang Surabaya. Methods for implementing activities include presentation, practice accompanied by a mentor, individual practice, and monitoring program sustainability. Based on the analysis of participants' ability, the participant scores before and after the activity was increase. It means that this training succeeded in increasing participants' knowledge and skills in processing waste, especially organic waste and used cooking oil waste. A total of 72 out of 116 participants (62%) had processed household waste either individually or in groups. Activity evaluation data shows that these activities are necessary and on target to create an independent and creative society.