Garuda - Garba Rujukan Digital

Application of chitosan scaffolds on vascular endothelial growth factor and fibroblast growth factor 2 expressions in tissue engineering principles Ariyati Retno Pratiwi; Anita Yuliati; Istiati Soepribadi; Maretaningtias Dwi Ariani
Dental Journal (Majalah Kedokteran Gigi) Vol. 48 No. 4 (2015): December 2015
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Background: Tissue engineering has given satisfactory results as biological tissue substitutes to restore, replace, or regenerate tissues that have a defect. Chitosan is an organic biomaterial often used in the biomedical field. Chitosan has biocompatible, antifungal, and antibacterial properties. Chitosan is osteoconductive, suitable for bone regeneration applications. Bone defect healing begins with inflammatory phase as a response to the presence of vascular injury, so new vascularization is required. Vascular endothelial growth factor (VEGF) and basic fibroblast growth factor-2 (FGF2) are indicators of the beginning of bone regeneration process, playing an important role in angiogenesis. Purpose: This research was aimed to determine the effects of chitosan scaffold application on the expressions of VEGF and FGF2 in tissue engineering principles. Method: Chitosan was dissolved in CH3COOH and NaOH to form a gel. Chitosan gel was then printed in mould to freeze dry for 24 hours. Those rats with defected bones were divided into two groups. Group 1 was the control group which defected bones were not administrated with chitosan scaffolds. Group 2 was the treatment group which defected bones were administrated with chitosan scaffolds. Those rats were sacrificed on day 14. Tissue preparations were made, and then immunohistochemical staining was conducted. Finally, a statistical analysis was conducted using Kruskal Wallis test. Result: There was no significant difference in the expressions of VEGF and FGF2 between the control group and the treatment group (p>0.05). Conclusion: Chitosan scaffolds do not affect the expressions of VEGF and FGF2 during bone regeneration process on day 14 in tissue engineering principles

Compressive strength and porosity tests on bovine hydroxyapatite-gelatin-chitosan scaffolds Nadia Kartikasari; Anita Yuliati; Indah Liatiana Kriswandini
Dental Journal (Majalah Kedokteran Gigi) Vol. 49 No. 3 (2016): September 2016
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Background: Degenerative diseases, aggressive periodontitis, trauma, jaw resection, and congenital abnormalities can cause defects in jaw bone. The surgical procedure for bone reconstruction currently performed is bone regeneration graft (BRG). Unfortunately, this procedure still has many disadvantages. Thus, tissue engineering approach is necessary to be conducted. The main component used in this tissue engineering is scaffolds. Scaffolds used in bone regeneration is expected to have appropriate characteristics with bone, such as high porosity and swelling ratio, low degradation rates, and good mechanical properties. For those reasons, this research used scaffolds made from bovine hydroxyapatite (BHA), gelatin (GEL), and chitosan (K)/BHA-GEL-K as one of biomaterial candidates for bone regeneration. Purpose: This study aimed to determine compressive strength value and porosity size of BHA-GEL-K scaffolds. Method: Compressive strength of BHA-GEL-K scaffolds was tested using autograph with speed 10 mm/ min with a load cell compress machine of 100 kN. Compressive strength was calculated by force divided to surface area. Porosity test was measured using SEM. Scaffold were coated with Pb and Au, then the porosity size is calculated with SEM at 100x magnification. Result: BHA-GEL-K scaffolds had a mean compressive strength value of 174.29 kPa and a porosity size of 31.62 + 147.06 lm. Conclusion: It can be concluded that BHA-GEL-K scaffolds has a good compressive strength, but not yet resemble real bone mass, while porosity of BHA-GEL-K scaffold is appropriate for bone tissue regeneration application.

Perubahan warna semen ionomer kaca setelah direndam dalam larutan teh hitam Wahyu Puri Wardhani; Asti Meizarini; Anita Yuliati; Retna Apsari
Journal of Dentomaxillofacial Science Vol. 9 No. 2 (2010): Formerly Jurnal Dentofasial ISSN 1412-8926
Publisher : DiscoverSys Inc.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15562/jdmfs.v9i2.242

Microporosity in glass ionomer cement contributes on its colour stability. In oralcavity GIC interacts with saliva, foods, drinks, and the oral hygiene condition whichcould affect in colour stability. This study aimed to examine the colour change in GICafter immersion in black tea solution. Twenty eight disc specimens (6 mm diameterand 1 mm thickness). The colour measurements were recorded after storage in closedtube for 24 hours. Specimens were randomly devided into 4 groups of 7 specimens.Control specimens were kept in saline solution. The other 3 groups were immersed inblack tea solution for 5 minutes respectively for 5, 7, and 14 times, followed by colourchange measurement. Data were submitted to one-way ANOVA and LSD. There wassignificant colour change in GIC after immersion in black tea. The conclusion islonger GIC immersed in black tea lower the intensity. The lowest intensity changingwas the immersed for 5 minutes, 14 times daily.

Sifat Fisik Permukaan Resin Komposit Hybrid Setelah Direndam dalam Minuman Energi pH Asam R Helal Soekartono; Anita Yuliati; Ratih Mutiara Sani; Dian Dwi Pratiwi
Jurnal Material Kedokteran Gigi Vol 3 No 1 (2014): JMKG Vol 3 No 1 Maret 2014
Publisher : Ikatan Peminat Ilmu Material dan Alat Kedokteran Gigi (IPAMAGI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (499.265 KB)

Composite resins are becoming more popular in restorative dentistry, particularly because of their superior esthetic properties. Composite resin may suffer degradation over time, which can be predicted by microstructural changes on its surface due to acidic conditions. The purpose of this study was to evaluate the microstructural changes of the surface of hybrid resin composite after immersed in acidic energy drinks of pH 3,2; 3,7; and 3,9 for 2 hours. Hybrid resin composites were cured with LED light curing unit. Specimens of 2 mm depth and 7 mm diameter were divided into 3 experimentals and 1 control. Each experimental was immersed in different energy drinks with different pH respectively (pH 3,2; 3,7; 3,9) and the control was immersed in aquadest for 2 hours. The surface roughness was measured using surface roughness measuring instrument before and after immersion then the Microstructural changes were evaluated under a scanning electron microscope (SEM). Surface roughness measuring instrument and SEM analysis showed, the erosion and surface degradation to all 3 hybrid resin composites after being subjected to the experimental conditions. The surface of hybrid resin composite undergoes noticeable microstructural changes after being immersed in energy drinks of pH 3.2, 3.7 and 3.9 for 2 hours.

PENGARUH RISIKO, MANFAAT, DAN KEMUDAHAN TERHADAP KEPUTUSAN MINAT BELANJA ONLINE Anita Yuliati; Makmur
Hirarki : Jurnal Ilmiah Manajemen dan Bisnis Vol. 5 No. 2 (2023): Hirarki : Jurnal Ilmiah Manajemen dan Bisnis
Publisher : Program Studi Manajemen Universitas Pasir Pengaraian

Show Abstract | Download Original | Original Source | Check in Google Scholar

This study aims to see how the influence of risks, benefits, and convenience on online shopping interest decisions in Sialang Rindang village. The population in this study are online shopping consumers in Sialang Rindang village. The sample used in this study was 95 people. The variables in this study are risk as a variable (X1), benefits as a variable (X2), convenience as a variable (X3), online shopping interest decisions as a variable (Y). Data collection techniques using a questionnaire. Data analysis used the SmartPLS 4.0.9.2 application program. The results obtained in this study are that the risk does not significantly influence the decision of online shopping interest, the benefits do not significantly influence the decision of online shopping interest, convenience has a significant effect on the decision of online shopping interest. The online shop needs to maintain or improve e-commerce services so that consumers still feel the convenience so that consumers will make purchases, especially in online shopping.

PENGARUH RISIKO, MANFAAT, DAN KEMUDAHAN TERHADAP KEPUTUSAN MINAT BELANJA ONLINE Anita Yuliati; Makmur
Hirarki : Jurnal Ilmiah Manajemen dan Bisnis Vol. 69 No. 69 (2025): CALVIN HARRIS
Publisher : Program Studi Manajemen Universitas Pasir Pengaraian

Show Abstract | Download Original | Original Source | Check in Google Scholar

This study aims to see how the influence of risks, benefits, and convenience on online shopping interest decisions in Sialang Rindang village. The population in this study are online shopping consumers in Sialang Rindang village. The sample used in this study was 95 people. The variables in this study are risk as a variable (X1), benefits as a variable (X2), convenience as a variable (X3), online shopping interest decisions as a variable (Y). Data collection techniques using a questionnaire. Data analysis used the SmartPLS 4.0.9.2 application program. The results obtained in this study are that the risk does not significantly influence the decision of online shopping interest, the benefits do not significantly influence the decision of online shopping interest, convenience has a significant effect on the decision of online shopping interest. The online shop needs to maintain or improve e-commerce services so that consumers still feel the convenience so that consumers will make purchases, especially in online shopping.

The characteristics of swelling and biodegradation tests of bovine amniotic membrane-hydroxyapatite biocomposite Agustantina, Titien Hary; Munadziroh, Elly; Yuliati, Anita; Bahtiar, Muhammad Riza Hafidz; Octarina; Salma, Rizki Fauziah; Meyranti, Ajeng Putri; Razak, Fathilah Abdul
Dental Journal (Majalah Kedokteran Gigi) Vol. 56 No. 3 (2023): September
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v56.i3.p172-177

Background: A good biocomposite is a structure that can provide opportunities for cells to adhere, proliferate, and differentiate. It is affected by the characteristics of a material. As bone tissue regeneration occurs, biomaterials must have a high swelling ability and low biodegradability. The high swelling capability will have a larger surface area that can support maximal cell attachment and proliferation on the biocomposite surface, which accelerates the regeneration process of bone defects. Purpose: The study aimed to analyze the characteristics of swelling and biodegradation of bovine amniotic membrane-hydroxyapatite (BAM-HA) biocomposite with various ratios. Methods: The BAM-HA biocomposite with a ratio of 30:70, 35:65, and 40:60 (w/w) was synthesized using a freeze-dry method. The swelling test was done by measuring the initial weight and final weight after being soaked in phosphate-buffered saline for 24 hours and the biodegradation test was done by measuring the initial weight and final weight after being soaked in simulated body fluid for seven days. Results: The swelling percentage of BAM-HA biocomposite at each ratio of 30:70, 35:65, and 40:60 (w/w) was 303.90%, 477.94%, and 574.19%. The biodegradation percentage of BAM-HA biocomposite at each ratio of 30:70, 35:65, and 40:60 was 9.43%, 11.05%, and 12.02%. Conclusion: The BAM-HA biocomposite with a ratio of 40:60 (w/w) has the highest swelling percentage while the 30:70 (w/w) ratio has the lowest percentage of biodegradation.

The compressive strength and static biodegradation rate of chitosan-gelatin limestone-based carbonate hydroxyapatite composite scaffold Rianti, Devi; Purnamasari, Alqomariyah Eka; Putri, Rifayinqa Ruyani; Salsabilla, Noor Zain; Faradillah; Munadziroh, Elly; Agustantina, Titien Hary; Meizarini, Asti; Yuliati, Anita; Syahrom, Ardiyansyah
Dental Journal (Majalah Kedokteran Gigi) Vol. 56 No. 3 (2023): September
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v56.i3.p160-165

Background: One of the main components in tissue engineering is the scaffold, which may serve as a medium to support cell and tissue growth. Scaffolds must have good compressive strength and controlled biodegradability to show biological activities while treating bone defects. This study uses Chitosan-gelatin (C–G) with good flexibility and elasticity and high-strength carbonate hydroxyapatite (CHA), which may be the ideal scaffold for tissue engineering. Purpose: To analyze the compressive strength and static biodegradation rate within various ratios of C–G and CHA (C–G:CHA) scaffold as a requirement for bone tissue engineering. Methods: The scaffold is synthesized from C–G:CHA with three ratio variations, which are 40:60, 30:70, and 20:80 (weight for weight [w/w]), made with a freeze-drying method. The compressive strengths are then tested. The biodegradation rate is tested by soaking the scaffold in simulated body fluid for 1, 3, 7, 14, and 21 days. Data are analyzed with a one-way ANOVA parametric test. Results: The compressive strength of each ratio of C–G:CHA scaffold 40:60 (w/w), 30:70 (w/w), and 20:80 (w/w), consecutively, are 4.2 Megapascals (MPa), 3.3 MPa, 2.2 MPa, and there are no significant differences with the p= 0.069 (p>0.05). The static biodegradation percentage after 21 days on each ratio variation of C–G:CHA scaffold 40:60 (w/w), 30:70 (w/w), and 20:80 (w/w) is 25.98%, 24.67%, and 20.64%. One-way ANOVA Welch test shows the result of the p-value as p<0.05. Conclusion: The compressive strength and static biodegradation of the C–G:CHA scaffold with ratio variations of 40:60 (w/w), 30:70 (w/w), and 20:80(w/w) fulfilled the requirements as a scaffold for bone tissue engineering.

Title

Found 18 Documents
Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title Search

Found 18 Documents Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 18 Documents
Search