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All Journal The Indonesian Biomedical Journal Acta Biochimica Indonesiana Jurnal Plastik Rekonstruksi The New Ropanasuri Journal of Surgery Jurnal Rekonstruksi dan Estetik
Yefta Moenadjat
Department Of Surgery, Faculty Of Medicine, Universitas Indonesia, Jakarta
Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Other

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Postoperative Wound Irrigation Using Distilled Water in Preventing Surgical Site Infection in a Tertiary Hospital: A retrospective Cohort and Cost-effective Study Jeo, Wifanto S.; Pratama, Dennis W.; Vanto, Yoni; Kekalih, Aria; Moenadjat, Yefta
The New Ropanasuri Journal of Surgery
Publisher : UI Scholars Hub

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Background. The incidence of surgical site infections (SSIs) is reducing following the global campaign that requires all the stakeholder involvement. However, of all hospital-acquired infection prevention programs, wound irrigation is hard to be implemented in our hospital. There is a belief that the wound irrigation procedure leading to the spreading of infection. In contrast, the use of antiseptic and topical antibiotic, as well as systemic antibiotic, is uncontrolled high. Thus, we run a cost-effective study of wound irrigation to change the practice. Method. We carried out a prospective cohort study comparing wound irrigation and the standard protocol in wound care in those underwent median laparotomy during the period of January to July 2018. A total of 80 subjects enrolled in this study, which divided into two groups, 40 for each group. The first group was those treated using antiseptics (povidone-iodine) and antibiotic contained paraffin tulle, while the second group was those treated using irrigation. This study performed in the digestive surgery division, which initiated irrigation protocol for wound irrigation. Stitch specimen taken for bacterial culture proceeded on 7th day postoperative and clinical signs of infection following CDC criteria was observed then statistically analyzed. The committee of ethics Faculty of Medicine Universitas Indonesia approved the study. Results. The bacterial culture showed no significant difference (p = 0.82) between the two groups. Clinical signs are showing no significant difference between the two groups (p = 1.00). In the cost perspective, the application of wound irrigation saving IDR 57,500,00 or four USD per subject. Conclusion. Wound irrigation using distilled water efficiently prevent SSIs.
Capsular Contracture, Prolonged Use of Breast Implants with Pleural Effusion: a Case Report Moenadjat, Yefta
The New Ropanasuri Journal of Surgery
Publisher : UI Scholars Hub

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A case with prolonged implanted breast prosthesis experienced a prolonged capsular contracture complicated with pleural effusion. A complete capsular removal proceeded instead of removal of the prosthesis solely. The present case aimed to share experiences that may add to the scientific horizon regarding the rare complications of breast prosthesis and rarely reported.
Minireview: Covid–19: Criticism of The Pathogenesis Based on The Tropism Moenadjat, Yefta
The New Ropanasuri Journal of Surgery
Publisher : UI Scholars Hub

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Academic Atmosphere in Training Program of Surgery Moenadjat, Yefta
The New Ropanasuri Journal of Surgery
Publisher : UI Scholars Hub

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Prediction of Liver Volume from Liver Transplant Donor using Biometric Formula compared with Computed Topography Volumetry Putranto, Agi S; Syafina, Adinda B; Sekarsari, Damayanti; Mazni, Yarman; Moenadjat, Yefta
The New Ropanasuri Journal of Surgery
Publisher : UI Scholars Hub

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Introduction. Liver volume calculation is critical in assessing the compatibility and resectability of the graft in living donor liver transplants (LDLT). An accurate estimation of liver volume is a predictor for successful LDLT. The gold standard of liver volume estimation is CT Volumetry. Despite several limitations in the availability of software, facility, and time consumed, there is still disagreement of biometric formula to predict liver volume in Indonesia. Methods: A cross-sectional design study was carried out in Dr. Cipto Mangunkusumo General Hospital, enrolling those who underwent liver transplantation from 1st January 2010 – 3rd October 2019. Bodyweight, body height, body mass index, body surface area, and CT volumetry were the variables of interest in the study and were subjected to analysis. Result. Body weight, body height, and body surface area are found from multivariate analysis in this research. Multivariate logistic regression of body weight with caudal liver volume giving out liver volume estimated equation of estimate liver volume of 479.23 + 13.95 (bodyweight). The equation in this study proposes a biometric formula to estimate liver volume using bodyweight based on Indonesian anthropometry. Conclusion: Bodyweight is proposed for equation formation based on a characteristic patient feature in Indonesia. Accuracy testing of the liver estimation equation discovered in this study proposed an entirely satisfactory result in the Indonesian population
The New Version of Accreditation for Higher Education in Health Moenadjat, Yefta
The New Ropanasuri Journal of Surgery
Publisher : UI Scholars Hub

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Prognostic and Predictive Factors of Mortality in Burn Patients at dr. Cipto Mangunkusumo General Hospital, Indonesia Hasibuan, Muhamad Irfan A; Moenadjat, Yefta
The New Ropanasuri Journal of Surgery
Publisher : UI Scholars Hub

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Introduction. Some prognostic– and predictive factors in burn have never been investigated in Indonesian specific characteristics, which is delayed treatment. A study was conducted to determine prognostic and predictive factors in this specific characteristic. Method. A retrospective cohort focused on burn mortality in extensive burns managed in CMGH from 1998–2010. Some known prognostic– and predictive factors and unknown ones were the variables of interest. Bivariate and multivariate using logistic regression analysis proceeded to find out these factors. Results. Out of all factors investigated, the variables that showed a significant correlation were: TBSA, inhalation injury, Baux score, revised Baux score, serum albumin, blood urea, serum creatinine, haemoconcentration, base excess, serum lactate, international normalized ratio, and random blood glucose. Conclusion. In the study, Baux score, revised Baux score, serum creatinine, haemoconcentration, base excess, and INR as predictive factors for mortality in the extensive burn.
ELISA method to detect ABO blood group in external secretion fluids Abdul Halim Sadikin; Yefta Moenadjat; Novi Sylvia Hardiany
Acta Biochimica Indonesiana Vol. 2 No. 1 (2019): Acta Biochimica Indonesiana
Publisher : Indonesian Society for Biochemistry and Molecular Biology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32889/actabioina.v2i1.33

Abstract

Background: Usually it takes a large number of volume sample to determine blood group from external secretion fluids. But, in certain condition, samples are only available in very small amount. The objective of this study is to detect the presence of ABO blood group substances in mucosal fluid using ELISA technique, thus only requires small amount of samples. Objective: To develop an ELISA technique using the current anti-ABO antibodies for determination of blood group by hemagglutination technique and second peroxidase label antibody specific for mouse IgG, originally used for another ELISA technique. Methods: 100 μl of diluted human intestinal mucosal fluid were incubated overnight in 4oC in ELISA microplate wells, followed by addition anti-ABO antibodies. Then after incubation, a second revealing antibody anti mouse IgG labeled with peroxidase was added. After a brief incubation, substrate H2O2 and chromogenic TMB were added. Results: Positive reaction is marked by development of blue colour, which, on termination enzymatic reaction by addition 100 μl H2SO4 change to yellow. Conclusion: An ELISA method for detecting ABO substance in mucosal fluid can be developed from antibodies not specifically made for this technique, but specific only for the target.
Paneth Cell Hyperplasia and Metaplasia in Hirschsprung-associated Enterocolitis in An Aganglionosis Rat Model Iskandar Rahardjo Budianto; Agus Firmansyah; Yefta Moenadjat; Ahmad Aulia Jusuf; Vivian Soetikno
The Indonesian Biomedical Journal Vol 14, No 4 (2022)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v14i4.2007

Abstract

BACKGROUND: Many hypotheses regarding the pathophysiology of enterocolitis in aganglionic megacolon or Hirschsprung disease (HSCR) has been proposed. Paneth cells are columnar intestinal epithelial cells that have an important role in maintaining of intestinal homeostasis as a bactericide. Since enterocolitis in HSCR may have association with Paneth cells metaplasia and hyperplasia, current study investigated Paneth cells metaplasia and hyperplasia in the sigmoid colon of HSCR rat model and its products, namely a-defensins and IL-1b, in the sigmoid colon tissues.METHODS: Aganglionosis-induced and control Sprague-Dawley rats were euthanized on Day (D)-7, -14, -17, -19, -21, -23, -25, and -28. Sigmoid colon tissue was isolated at each time point, and degree of enterocolitis as well as Paneth cells metaplasia and hyperplasia were analyzed by Hematoxylin-eosin staining, then protein levels of a-defensins and interleukin (IL)-1b were determined by enzyme-linked immunosorbent assay (ELISA).RESULTS: Enterocolitis scores increased with time. The Paneth cells metaplasia and hyperplasia were observed on D14 until D28 (p<0.01 vs. control group) followed by an increased in the levels of IL-1b. The levels of a-defensins protein expression were initially increased (D7-D14; p<0.01 vs. control group) but then undergo reciprocal changes on D19 until D28 (p<0.01 vs. D7 and D14). Positive correlations between the degree of enterocolitis and Paneth cells number were detected in the sigmoid colon (r=0.42).CONCLUSION: Paneth cells underwent metaplasia and hyperplasia in the sigmoid colon of HSCR rats corresponding to an increase in the degree of enterocolitis, but not followed by an increase in the level of a-defensins as well as IL-1b, suggesting that there is an involvement of Paneth cells in the pathophysiology of enterocolitis due to HSCR.KEYWORDS: Hirschsprung, enterocolitis, defensins; metaplasia, Paneth cell, animal model
EARLY CLEFT LIP REPAIR: A LONG TERM FOLLOW-UP Moenadjat, Yefta
Jurnal Rekonstruksi dan Estetik Vol. 9 No. 2 (2024): Jurnal Rekonstruksi dan Estetik, December 2024
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jre.v9i2.60219

Abstract

Highlights: Early cleft repair benefits patients by reconstructing near-normal lip anatomy that persists after ten to fifteen years. The early procedure did not result in muscular or maxillary hypoplasia in long-term follow-ups; rather, development occurred in tandem with normal growth. Abstract: Introduction:  Published reports on the long-term outcomes of cleft lip surgery within the Indonesian population are limited, especially for those treated early in the neonatal period. This study presents follow-up data on early-managed cases, aiming to objectively evaluate the long-term results of such management. Case Illustration: A newborn with a complete unilateral cleft lip and palate had surgery on day two, achieving a symmetrical lip and nasal base. At 12 months, cleft palate surgery was performed. Fifteen years later, the patient sought a lip revision, expressing satisfaction with the results and opting against further procedures. Case 2: Another newborn with an incomplete cleft underwent surgery on day five, followed by palate repair at 12 months. Ten years later, the parents reported satisfaction with the outcomes and no desire for further revisions. Discussion: The discussion focused on the merit of early management and no negative impact on muscular and maxillary growth. Conclusion: The positive effects of early management through muscle management were observed in the long-term follow-up, as shown in these two reported cases.
Co-Authors Abdul Halim Sadikin Agus Firmansyah Ahmad Aulia Jusuf Anis Karuniawati Aria Kekalih Atmodiwirjo, Parintosa Benjamin Ngatio, Benjamin Camy F. Surjadi Caroline T. Sardjono Dalima A.W. Astrawinata Damayanti Sekarsari Ferry Sandra Handaya, Adeodatus Y. Hasibuan, Muhamad Irfan A Indah S. Widyahening Iskandar Rahardjo Budianto Ismet, Mohamad F. Iswanto, J. Jeanne A. Pawitan Jeo, Wifanto S. Jeo, Wifanto Saditya Kusnadi, Dana S. Lalisang, Toar JM. Lia Damayanti Marini Stephanie Maulanisa, Sinta C. Maurin Merlina Mazni, Yarman Mohamad Rachadian Ramadan Mohamad Sadikin Mulya, Dina Mulya, Dina D. Nasution, Safruddin Novi Sylvia Hardiany Nurjati C. Siregar Pratama, Dennis W. Puspasari, Antonia Valentine Putranto, Agi S Putranto, Agi S. Radiana D. Antarianto Ramdhani, Aris Retno Asti Werdhani Rianto Setiabudy Robby, Rizky DK. Saukani Gumay Saunar, Rofi Y. Saunar, Rofy S. Septelia I. Wanandi Suharto, Wuryantoro Syafina, Adinda B Toar J.M. Lalisang Tony Loho Twidy Tarcisia, Twidy Usman, Nurhayat Vanto, Yoni Vivian Soetikno Yuyus Kusnadi