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Inoculation of Endophytic Bacteria for Increasing Plant Height and Number of Rice Roots (Oryza sativa) Fadilah, Fari; Rahayu, Triastuti; Agustina, Putri; Mumpuni, Kistantia Elok; Mandiri, Listy Hasti; Prayitno, Trio Ageng; Sidiq, Yasir
Bioscientist : Jurnal Ilmiah Biologi Vol. 11 No. 2 (2023): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v11i2.8642

Abstract

Four endophytic bacteria have been isolated from banana kluthuk plants and banana ambon. Previous study showed that they produce Indole Acetic Acid (IAA). This study aimed to analyze the effect of endophytic bacterial inoculation on plant height and the number of rice root. For this reason, two endophytic bacteria of each klutuk banana and ambon banana plants were cultured and examined for growth rate using a 600 nm spectrophotometer. Isolate growth was calculated every two hours for 16 hours of bacterial growth in NB media. Bacterial inoculation experiments on rice plants were carried out with five replications. This inoculation begun with germination of rice seeds until the radicle was observed. Then germinated rice seedlings were soaked in a bacterial suspension for 1 hour at room temperature. The results showed the exponential phase of all isolates was observed at 6 hours with shaking. Inoculation using A22 and A51 isolates significantly increases plant height, number of leaves. While, A22 isolate significantly increased the number of roots compared to the uninoculated plants (control). It supports the potential of endophytic bacteria from banana kluthuk and ambon plants.
Self-regulated Learning Questionnaire: Differential Item Functioning (DIF) and Calibration using Rasch Model Analysis Mumpuni, Kistantia Elok; Begimbetova, Guldana Atymtaevna; Retnawati, Heri
Jurnal VARIDIKA Volume 35, No 1 June 2023
Publisher : Faculty of Teacher Training and Education, Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/varidika.v1i1.22995

Abstract

Questionnaires are commonly utilized on educational research. However, studies on Differential Item Functioning (DIF) and calibration using Rasch Models are still limited. Therefore, a Self-regulated Learning questionnaire was developed which aims to determine the ability of students to regulate themselves to achieve learning goals. The instrument consists of twelve items. The involved participants were 300 students who enrolled in first-year to fourth-year. Data were analyzed using Racsh Model Analysis with Winsteps 4.5.2 software. As a result, there are four items that were not fit, so that, therefore should be eliminated or revised. The DIF analysis found that gender bias was unidentified, but long-study bias was detected for items number one and six. The reliability value of the item is categorized as very good (0.99), which indicates that the instrument has sufficient consistency/reliability. While, the function curve showed that the items on the self-regulated learning questionnaire produce optimal information in individuals with moderate (θ) ability. Overall, self-regulated learning questionnaires have to be revised then tested on different sample groups. In addition, longitudinal and cross-sectional research is necessary to determine the level of self-regulated learning of students more comprehensively.
Rhizosphere Bacteria Plumeria acuminata Increases Growth of Zea mays After Root Inoculation Insani, Qori Tsaniyah Ainun; Rahayu, Triastuti; Widianto, Donny; Listiawati, Vina; Mumpuni, Kistantia Elok; Prayitno, Trio Ageng; Sidiq*, Yasir
Jurnal IPA & Pembelajaran IPA Vol 7, No 4 (2023): DECEMBER 2023
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jipi.v7i4.34658

Abstract

The bacteria found in the rhizosphere are called plant growth promoting rhizobacteria (PGPR), namely bacteria that can stimulate plant growth by producing growth hormones, one of which is the hormone indole acetic acid (IAA) which plays a role in regulating plant growth and development. Five isolates of rhizosphere bacteria were isolated on frangipani roots (P26, P31, P36, P37 and P38). Previous research showed that five bacterial isolates were capable of producing the hormone IAA. However, evidence of its potential as a PGPR has not been obtained. Therefore, the aim of this study was to test the effect of inoculation of rhizosphere bacteria on maize plants. The research method uses a quantitative experimental method, namely 15 repetitions for each treatment. Data collection was carried out 1 day after planting (HTS) until the 14th day. Data were analyzed using a 95% confidence student t-test or with a significance level of 5% (a=0.05). This inoculation begins with germinating maize seeds and observing the roots. The germinated maize seeds are then soaked in the bacterial suspension for one hour at room temperature. The results showed that inoculation with isolates P36, P37 and P38 significantly increased the height of maize plants, while isolate P37 significantly increased the number of leaves and root length of corn plants, isolate P38 could significantly increase the number of roots of corn plants.Thus, these results support the evidence that rhizosphere bacteria from frangipani have an effect on increasing the growth of maize plants