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Identification of plasmodium falciparum and plasmodium vivax on digital image of thin blood films gf Hanung Adi Nugroho; Made Satria Wibawa; Noor Akhmad Setiawan; E. Elsa Herdiana Murhandarwati; Ratna Lestari Budiani Buana
Indonesian Journal of Electrical Engineering and Computer Science Vol 13, No 3: March 2019
Publisher : Institute of Advanced Engineering and Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11591/ijeecs.v13.i3.pp933-944

Abstract

Observing presence of Plasmodium parasite of stained thick or thin blood films through microscopic examination is a gold standard for malaria diagnosis.  Although the microscopic examination has been extensively used, misidentification might occur caused by human factors.  In order to overcome misidentification problem, several studies have been conducted to develop a computer-aided malaria diagnosis (CADx) to assist paramedics in decision-making.  This study proposes an approach to identify species and stage of Plasmodium falciparum and Plasmodium vivax on thin blood films collected from the Laboratory of Parasitology, Faculty of Medicine, Universitas Gadjah Mada.  Adaptive k-means clustering is applied to segment Plasmodium parasites.  A total of 39 features consisting of shape and texture features are extracted and then selected by using wrapper-based forward and backward directions.  Classification is evaluated in two schemes.  The first scheme is to classify the species of parasite into two classes. The second scheme is to classify the species and stage of parasite into six classes.  Three classifiers applied are k-nearest neighbour (KNN), support vector machine (SVM) and multi-layer perceptron (MLP).  Furthermore, to facilitate the multiclass classification, one-versus-one (OVO) and one-versus-all (OVA) methods are implemented.  The first scheme achieves the accuracy of 88.70% based on MLP classifier using three selected features.  While the accuracy gained by the second scheme is 95.16% based on OVO and MLP classifier using 29 selected features.  These results indicate that the proposed approach successfully identifies the species and stage of parasite on thin blood films and has potential to be implemented in the CADx system for assisting paramedics in diagnosing malaria.
Intestinal Helminth Infections among Children with Disabilities in Bantul Regency, Special Region of Yogyakarta Lala Foresta Valentine Gunasari; Elsa Herdiana Murhandarwati
JUMANTIK (Jurnal Ilmiah Penelitian Kesehatan) Vol 6, No 4 (2021)
Publisher : Prodi Kesehatan Masyarakat Fakultas Kesehatan Masyarakat UIN Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (394.418 KB) | DOI: 10.30829/jumantik.v6i4.9892

Abstract

Due to physical, intellectual, mental, and/or sensory limitations, person with disabilities tend to experience obstacles and limitations in their ability to care for themselves, knowledge of health, and access to health services. As a result, people with disabilities are more at risk of experiencing health problems, one of which is intestinal helminth infection. The aim of this study was to assess the prevalence of intestinal helminth infections and associated risk factors among children with disabilities at school age in Bantul Regency, Special Region of Yogyakarta. A school-based cross-sectional study design was conducted from October until December 2019. A total of 130 stool samples were collected and examined by the flotation, Kato-Katz, Harada-Mori, and Baermann methods to detect the presence of helminth eggs and/or larvae. An interview with parents/guardians of the subject based on a questionnaire was conducted to obtain information regarding the associated risk factor. The overall prevalence of intestinal helminth infections among subjects was 1.5% (CI=95%). Of these, 0,07% (1/130) was positive for hookworm infection, and the remaining 0,07% (1/130) was positive for Trichuris trichiura. Analysis for all risk factors showed no statistically significant association between all risk factors and the intestinal helminths infections in the study subjects (p>0.05).
HUBUNGAN JENIS BREEDING SITES DAN KEPADATAN LARVA DENGAN KASUS MALARIA DI WILAYAH PT. FREEPORT INDONESIA KABUPATEN MIMIKA TAHUN 2013-2017: PENDEKATAN SPASIAL-TEMPORAL Wafiyyah Rizki Wiariyanti; E. Elsa Herdiana Murhandarwati; Anis Fuad
Berita Kedokteran Masyarakat (BKM) Vol 35, No 10 (2019)
Publisher : Fakultas Kedokteran Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (512.551 KB) | DOI: 10.22146/bkm.47232

Abstract

Breeding sites and larvae density with malaria cases in PT. Freeport Indonesia, mimika regency in 2013-2017: spatio-temporal approachPurpose: This study aims to determine the relationship between breeding sites and larval density in malaria cases in the Lowland region of PT. Freeport Indonesia Mimika Regency 2013-2017. Method: This study uses secondary data from PT. Freeport Indonesia in 2013 - 2017. The data used in this study included malaria case data, breeding sites and breeding sites and larval density in the lowland PTFI area from 2013-2017. Data analysis included descriptive analysis to see the distribution picture, bivariate analysis to examine the relationship between variables and spatial analysis to find out the distribution of cases and breeding sites.Results: The number of malaria cases from 2013-2017 in the Lowland PTFI region was 267 (37%) from a total population of 713 people. The highest case occurred in 2014 as many as 70 cases. Distribution of cases based on sex occurs mostly in men, in the age group of 46-50 years, based on the relatively equal employment status between employees and non-employees and based on the location, many occur in Kuala Kencana. Types of breeding sites with larvae density have a significant relationship (p <0.0001), larvae density with malaria cases also have a significant relationship (p 0.0013 and r 0.40) especially density in xpool which has a density of 2.01/dip.Conclusion: Distribution of malaria cases focused on locations where employees live and locations with high mobility. The types of breeding sites are related to larval density. The highest density of larvae is found in breeding sites of the xpool type. Larval density and malaria cases have a significant relationship with larvae density in the xpool breeding site.
Pemetaan Spasial Malaria dan Faktor Risiko di Kecamatan Lamboya Kabupaten Sumba Barat Agustina Mbiliyora; Tri Baskoro Tunggul Satoto; Elsa Herdiana Murhandarwati
Jurnal Kesehatan Vokasional Vol 8, No 4 (2023): November
Publisher : Sekolah Vokasi Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jkesvo.88985

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Latar Belakang: Malaria adalah penyakit tular vektor yang masih menjadi masalah utama kesehatan masyarakat di Kecamatan Lamboya Sumba Barat. Analisis spasial diperlukan untuk mengetahui sebaran kasus malaria, penentuan wilayah receptive dan vulnerable.Tujuan: Penelitian bertujuan untuk mengetahui pola sebaran kasus malaria, tipe habitat jentik, pemetaan wilayah receptive dan vulnerable dan pengaruh faktor risiko di Kecamatan LamboyaMetode: Penelitian ini menggunakan rancangan unmatched case control. Besar sampel masing-masing 36 kontrol responden positif dan negatif malaria Januari 2023. Data diperoleh melalui e-SISMAL (Elektronik Sistem Informasi Surveilans Malaria) Puskesmas Kabukarudi dan Klinik Malaria. Analisis data meliputi analisis spasial dengan QGIS, univariat dan bivariat dengan uji chi-square. Wilayah receptive ditentukan berdasarkan temuan kasus malaria dan habitiat positif jentik, vulnerable wilayah tanpa kasus namun berbatasan langsung dengan wilayah receptiveHasil: Sebaran malaria berpola random dekat habitat nyamuk radius 500m ada 25 kasus dan radius 1000m ada 6 kasus. Habitat positif jentik pada sawah, mata air, muara, sungai, genangan dan kubangan. Lima wilayah receptive pada Patiala Bawa, Palamoko, Lamboya Bawa, Ringurara dan Watukarere. Tiga wilayah vulnerable Wailibo, Kabukarudi dan Rajaka. Hasil identifikasi nyamuk ditemukan An.Vagus, An.Limosus, An.Subpictus, An. Indefinitus dan An.Annularis.Kesimpulan: Pola sebaran malaria menyebar mendekati habitat nyamuk. Ditemukannya nyamuk potensial vektor malaria dan habitat positif jentik menunjukkan bahwa di Kecamatan Lamboya berpotensi tinggi terjadinya penularan malaria.
Gender related factors affecting implementation fidelity of Mass Drug Administration of Lymphatic Filariasis Programme in Jhapa district of Nepal: An Implementation Research Shreya Khanal; Elsa Herdiana Murhandarwati; Retna Siwi Padmawati
BKM Public Health and Community Medicine The 12th UGM Public Health Symposium
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Purpose: This study explores gender related factors affecting implementation fidelity of Mass Drug Administration (MDA) of lymphatic filariasis programme in Jhapa, Nepal. Method: Implementation research was conducted using an explanatory sequential design. For quantitative study, valid secondary data was used. For qualitative study, primary data will be collected from 46 respondents including community members and healthcare providers. Result: Only 77.66% epidemiological coverage was reached during MDA conducted in 2023. Both males and females over the age of two were included in the coverage, with females making up 51.01%. The lowest coverage of 46.24% and 79.81% was found among each urban and rural municipalities, respectively. Further, the qualitative data expects to identify underlying gender-related factors justifying the inequitable coverage among municiplaities, between gender and age groups that influences potential moderators in accessing resources, program adherence influenced by gender roles, social norms and values, and decision-making power in healthcare among men and women in urban and rural areas. Thus, the study results expects to provide crucial insights into how gender interacts with potential moderators of implementation fidelity in programme adherence. A study in Nepal's western terai region found that educated individuals, men working away from home as the household breadwinner, elderly individuals, urban communities, and those with chronic illnesses are more likely to miss or refuse mass drug administration (MDA) due to concerns about drug safety and lack of knowledge. Some community members also lack trust in female community health volunteers due to their lower levels of education and training, leading to lower program adherence rates due to persistent gender barriers. Conclusion: The findings obtained from this study will assist in making evidence-based decisions to address gender disparities and inequitability in accessing and utilizing interventions implemented by national neglected tropical disease programs.
Correlation of Nutritional Status with Hookworm and Strongyloides stercoralis Infection in Children Under Five Years in Kokar Public Health Center, Alor Regency, East Nusa Tenggara Onesiforus, Benaya Yamin; Lalangpuling, Indra E.; Wijayanti, Mahardika A.; Murhandarwati, E. Elsa Herdiana
Indonesian Journal of Tropical and Infectious Disease Vol. 8 No. 3 (2020)
Publisher : Institute of Topical Disease Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ijtid.v8i3.12570

Abstract

Malnutrition can reduce immune response particularly in cytokine (IL-4, IL-5, IL-10) production and immune effector (eosinophil, IgE, and mast cell), thus increasing the probability of intestinal nematode infection. Through this study, intestinal nematode infections occurred among children under five years, at different nutrition status, in Kokar Public Health center, Alor Regency, East Nusa Tenggara was captured. Hookworm and Strongyloides stercoralis were studied as both of them have devastating impacts compare to other helminthes compare to other helminths. This study is a cross- sectional study with a quote sampling technique. As many as 238 children, aged 12-59 months living in Kokar's Public Health Center area, Alor regency were recruited in this study i.e. 7.7% severely underweight, 19.2% underweight, 70.5% normal and 2.6% overweight. Data were collected in August - October 2016. Hookworm and S. stercoralis infection were determined from collected fecal samples of all subjects using either Baermann test, Koga Agar Plate (KAP), or Harada- Mori culture method. The prevalence of hookworm and S. stercoralis infection was 8.82%, and 0,42%. Correlation between nutritional status and hookworm infection were analyzed by Mann-Whitney test with p value = 0.54 (p > 0.05). Prevalence of hookworm and S. stercoralis among children under five years in Kokar were 8.82% and 0.42%. There was no significant correlation between nutritional status with hookworm infection prevalence.
STUDENTS’ PERCEPTION ON THE USE OF A MASSIVE OPEN ONLINE COURSE FOR PARASITOLOGY: A PRELIMINARY STUDY Utomo, Prattama Santoso; Kusumasari, Rizqiani Amalia; Murhandarwati, Elsa Herdiana; Widyandana, Doni
Jurnal Pendidikan Kedokteran Indonesia: The Indonesian Journal of Medical Education Vol 12, No 2 (2023): Juni
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpki.82183

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Background: The COVID-19 pandemic had a significant impact on medical education globally. The learning process has been shifted to online learning to ensure health and safety measures. Online learning approaches and applications are also established rapidly, including the Massive Open Online Course (MOOC), which can support learning in many topics and facilitate a large number of students effectively and efficiently. Department of Parasitology and Department of Medical Education and Bioethics, Faculty of Medicine, Public Health, and Nursing, Universitas Gadjah Mada developed the Central Nervous System (CNS) Parasitic Infection module as an online learning for both undergraduate and postgraduate levels.Aims: The study aimed to evaluate and explore students’ perception of the CNS Parasitic Infection online module development in an MOOC format.Methods: This is an evaluation study using an explorative qualitative approach. The pilot implementation of the CNS Parasitic Infection module was evaluated using in-depth interviews with ten (10) students of the Master in Tropical Medicine and the Master in Biomedical Science programs. The participants were requested to share their learning experience on the module, and their suggestions for module improvement. The interviews were conducted online using Zoom. The interview transcripts were analyzed using thematic analysis.Results: Implementing the CNS Parasitic Infection module using MOOC asynchronous approach received both positive and negative responses from participants. A total of 6 themes were identified from participants, for instance, learning outcomes achievement, interactive e-case design, learning flexibility, course timing, network issues, and the need for learning directions.Conclusion: CNS Parasitic Infection can be delivered to students using an asynchronous MOOC format. Students’ outcomes achievement was sufficient using this method, and overall, students appreciated the implementation of the program. 
Analisis Indikator Entomologi dan Sebaran Jentik Aedes aegypti pada Daerah Stratifikasi Endemisitas Demam Berdarah Dengue di Kota Jayapura: Analysis of Entomology Indicator and distribution of Aedes aegypti vector based on stratified Endemicity Areas of Dengue Hemorrhagic Fever (DHF) in Jayapura City Hartati, Risda; Satoto, Tri Baskoro T.; Murhandarwati, Elsa Herdiana; Widawati, Mutiara
Aspirator Vol 13 No 2 (2021): Jurnal Aspirator Volume 13 Nomor 2 2021
Publisher : Perkumpulan Entomologi Kesehatan Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/asp.v13i2.4441

Abstract

Abstract. The incidence rate (IR) of Dengue Hemorrhagic Fever in Papua Province in 2017 was8.04 per 100,000 population, with a case fatality rate (CFR) of 1.12%. In 2016, the Health Office ofJayapura City declared six urban villages as high endemic areas, four urban villages as high sporadicareas, and ten villages as DHF-free areas from a total of 39 urban villages in Jayapura. This studyaims to analyze larval by House index [HI], Container index [CI], Breateu index (BI), and Maya index[MI] as well as the distribution of vector Ae. aegypti in endemic, sporadic and DHF-free areas inJayapura City. The design of this study was a cross-sectional study. This research was conducted fromJanuary to March 2019. Subjects (375 houses) were surveyed according to WHO guidelines basedon its endemicity stratification. Observations of mosquitos’ larvae were done using visual and singlelarvae methods. The result of this research showed that endemic areas had their HI, CI and BI as muchas 43.3%, 16.5%, and 90.0%, respectively, with density figure is six. Sporadic areas had their HI,CI and BI as much as 35.4%, 10.9%, and 57.5%, respectively, with density figure is five. DHF-freeareas had their HI, CI, and BI as much as 14.8%, 5.7%, and 35.2%, respectively, with density figureis five. The Maya index for endemic and sporadic areas was categorized as moderate, while DHF-freeareas were low. Abstrak. Angka incidence rate (IR) Demam Berdarah Dengue (DBD) Provinsi Papua tahun2017 adalah 8,04 per 100.000 penduduk, dengan case fatality rate (CFR) sebesar 1,12%.Pada tahun 2016 Dinas Kesehatan Kota Jayapura menetapkan 6 kelurahan sebagai daerahendemis tinggi, 4 kelurahan sebagai daerah sporadis tinggi dan 10 kampung sebagai daerahbebas DBD dari total 39 kelurahan yang ada di Kota Jayapura. Tujuan penelitian ini untukmenganalisis kepadatan jentik menggunakan House Index (HI), Container Index (CI), BreateuIndex (BI), dan Maya Index (MI) serta menganalisis sebaran jentik Ae.aegypti di daerahendemis, sporadis dan bebas DBD di Kota Jayapura. Penelitian dilakukan dari Januari-Maret2019 dengan menggunakan metode potong lintang. Subyek penelitian 375 rumah disurveimenurut pedoman WHO menurut stratifikasi endemisitasnya. Observasi jentik nyamukdengan metode visual dan single larvae. Hasil penelitian ini menunjukkan bahwa indeksentomologi daerah endemis dengan nilai HI 43,3%, CI 16,5%, BI 90,0% dengan Density Figure(DF) 6 (tinggi). Sporadis: HI 35,4%, CI 10,9%, BI 57,5%, dengan density figure 5. Bebas DBD HI14,8%, CI 5,7% dan BI 35,2% dengan DF 5 (sedang). Status MI kategori sedang pada daerahendemis dan sporadis dan MI rendah pada daerah bebas DBD.