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Journal : LINK

Effectiveness of Steriliztion Infrared Dry Heat Sterilization and Sterility Of Dentistry Tools Sariyem Sariyem; Sadimin Sadimin; Prasko Prasko
Jurnal LINK Vol 9, No 1 (2013): Januari 2013
Publisher : Pusat Penelitian dan Pengabdian kepada Masyarakat, Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (6507.365 KB) | DOI: 10.31983/link.v9i1.166

Abstract

Transmission of infection can be occured  from the use of instruments that are not sterile, to break the chain of transmission of the infection, it is necessary to carry out the sterilization of medical devices with the correct procedures such as sterilization by dry heat or infrared can also with other methods. The purpose of this study to know the number of microorganisms and decrease the effectiveness of both the sterilization sterilize dental instruments.The reacherd was conducted in the laboratory using the method of experimental research, design research is one group pretest-posttest. The data have been acquired and processed statistically tested by Paired T-Test and Independent Test.Based on the results, the data is a decline in the number of bacteria in the infrared sterilization, but still found breeding of bacteria in sterilized tools. Decline in the number of bacteria in dry heat sterilization and no breeding of bacteria in all the tools are sterilized. The results of statistical tests Paired T-Test were both obtained p value = 0.000, so it can be said that the infrared sterilization and dry heat sterilization is effective in reducing the number of bacteria on equipment in dentistry. The statistical result obtained Independent T-Test p value: 0.626, which means there are no significant differences between the infrared sterilization and dry heat sterilization in killing microorganisms.
KEMAMPUAN OBAT KUMUR EKSTRAK JINTEN HITAM SEDIAAN KANTONG CELUP TERHADAP MONOSIT DAN NEUTROFIL PADA ADHESI STREPTOCOCCUS MUTAN Supriyana Supriyana; Endah Aryati; Sadimin Sadimin; Wahyu Jati Dyah Utami
Jurnal LINK Vol 15, No 2 (2019): NOVEMBER 2019
Publisher : Pusat Penelitian dan Pengabdian kepada Masyarakat, Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (237.124 KB) | DOI: 10.31983/link.v15i2.5384

Abstract

Jintan hitam mengandung senyawa antimikroba bersifat volatil dan non volatil dengan bermacam tingkat kepolaran. Penelitian sebelumnya dikemukakan semakin lama perendaman kantong celup berisi bahan herbal, kadar klorinnya semakin rendah, terutama pada perendaman kantong celup berisi ekstrak flavonoid dari jinten hitam. Adanya kandungan flavonoid ekstrak jinten hitam dan klorin yang dikeluarkan oleh kantong celup, perlu diketahui bagaimana ekstrak jinten hitam dengan sediaan kantong celup mempengaruhi respon inflamasi sel radang neutrofil yang sebelumnya dipapar Streptokokus mutan secara in-vitro. Penelitian ini bersifat eksperimental laboratories, rancangan penelitian menggunakan the post test only control group design. Populasi penelitian ekstrak flavonoid jinten hitam dengan sediaan kantung celup jumlah sampel minimal 4 sampel untuk tiap kelompok perlakuan. Sampel dikelompok menjadi 10 kelompok yaitu 5 uji adhesi neutrofil dan 5 uji monosit. Hasil perhitungan viabilitas monosit yang dipapar S. mutans menunjukkan bahwa jumlah monosit yang viabel (hidup) pada inkubasi air seduhan 8 menit kantung teh celup ekstrak flavonoid jinten hitam paling tinggi, dan kelompok kontrol (kantung kosong) paling rendah, dan menunjukkan perbedaan yang signifikan antar kelompok perlakuan (p0.05). Obat kumur dengan sediaan teh celup berisi ekstrak flavonoid jinten hitam mampu meningkatkan adhesi S mutans pada neutrofil, sehingga neutrofil sebagai sel imunitas dapat melakukan fagositosis dan membunuh S mutans.