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The Synergy of Recombinant Xylanolytic Enzyme on Xylan Hydrolysis Asmarani, One; Wardojo, Bambang Prajogo Eko; Puspaningsih, Ni Nyoman Tri
Makara Journal of Technology Vol. 15, No. 1
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Microbial xylanases or xylanolytic enzyme have received considerable attention over the last years owing to a multitude of possible applications. These enzymes have potential in the biodegradation of lignocellulosic biomass to fuels, chemicals, fruit juice, animal feed and in improving rumen digestion. More recently, the use of xylanases as bleaching agent in the pulp and paper industry has been suggested to replace of some of the chemicals presently used for this purpose. Such applications could have an important positive impact on the environment. The purpose of this research was determining the synergy of 3 recombinant xylanolytic enzymes (β-xylosidase, exo-xylanase and α-Larabinofuranosidase) from recombinant Eschericia coli BL21 (DE-star) in xylan hydrolysis by analysis the reduction sugar product. Purified of recombinant xylanolytic enzyme β-xylosidase (Xyl), exo-xylanase (Exo-Xyl) and α-Larabinofuranosidase (Abfa) with Ni-NTA resin. Seven samples of enzyme (each and enzyme mixture) used to hydrolyze xylan substrate (oat-spelt xylan). Analysis of hydrolysis product was done by HPLC. The xylanolytic activities of this enzyme before and after purification were 0,91 and 9,94 U/mL (Exo-Xyl); 1,65 and 14,2 U/mL (Xyl); 0,65 and 5,6 U/mL (Abfa). The xylosidase activity were 2,37 and 14,3 U/mL (Xyl); 1,49 and 10,5 U/mL (Exo-Xyl); 2,54 and 18,6 U/mL (Abfa). The highest hydrolysis product of xylan (xylose) shown in enzyme mixture of exoxylanase and β-xylosidase was 1,084 mg/mL.

Studi Profil Metabolit Pada Organ Tikus Hasil Uji Toksisitas Sub Kronis Ekstrak Etanol 70% Daun Justicia Gendarussa Nur Irhamni Sabrina; Luthfiatu Kanina; Bambang Prajogo EW; Retno Widyowati; Suciati Suciati
Jurnal Serambi Engineering Vol 8, No 1 (2023): Januari 2023
Publisher : Fakultas Teknik

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32672/jse.v8i1.5580

AbstractJusticia gendarussa Burm.f has been traditionally used as male contraceptionin in Indonesia. Gandarusa leaves contain gendarusin A which has potential as an antifertility agent. To prove that gandarusa leaves are safe to use as a male contraceptive, a subchronic toxicity test was carried out on this plant. Acute and subchronic oral toxicity studies of Justicia gendarussa Burm.f in rats were performed in the previous study in order to evaluate its safety. The aim of this study is to identify the metabolite profile of rat organ results of their subchronic toxicity test. Seven organs of rat such as kidney, hepar, testis, limph, instestine, lung, and heart were extracted using methanol and their profile metabolites were identified using UHPLC Vanquish Tandem Q Exactive Plus Orbitrap HRMS by positive and negatif mode. The results showed that all sampels contain 22 compounds from the positive ionization mode and 35 compounds from the negatif ionization mode. Compounds identified from the positive mode were dominated by amino acid groups, then compounds identified from the negatif mode were dominated by fatty acids and their derivatives. There were 35 metabolites that identified from the organ samples of the rat resulting subchronic toxicity test using the UHPLC Vanquish Tandem Q Exactive Plus Orbitrap HRMS method.Keywords: Justicia gendarussa Burm.f, rats, organ, metabolite profile

Studi Profil Metabolit Pada Organ Tikus Hasil Uji Toksisitas Sub Kronis Ekstrak Etanol 70% Daun Justicia Gendarussa Nur Irhamni Sabrina; Luthfiatu Kanina; Bambang Prajogo EW; Retno Widyowati; Suciati Suciati
Jurnal Serambi Engineering Vol 8, No 1 (2023): Januari 2023
Publisher : Fakultas Teknik

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32672/jse.v8i1.5580

AbstractJusticia gendarussa Burm.f has been traditionally used as male contraceptionin in Indonesia. Gandarusa leaves contain gendarusin A which has potential as an antifertility agent. To prove that gandarusa leaves are safe to use as a male contraceptive, a subchronic toxicity test was carried out on this plant. Acute and subchronic oral toxicity studies of Justicia gendarussa Burm.f in rats were performed in the previous study in order to evaluate its safety. The aim of this study is to identify the metabolite profile of rat organ results of their subchronic toxicity test. Seven organs of rat such as kidney, hepar, testis, limph, instestine, lung, and heart were extracted using methanol and their profile metabolites were identified using UHPLC Vanquish Tandem Q Exactive Plus Orbitrap HRMS by positive and negatif mode. The results showed that all sampels contain 22 compounds from the positive ionization mode and 35 compounds from the negatif ionization mode. Compounds identified from the positive mode were dominated by amino acid groups, then compounds identified from the negatif mode were dominated by fatty acids and their derivatives. There were 35 metabolites that identified from the organ samples of the rat resulting subchronic toxicity test using the UHPLC Vanquish Tandem Q Exactive Plus Orbitrap HRMS method.Keywords: Justicia gendarussa Burm.f, rats, organ, metabolite profile

THE EFFECT OF GENDARUSSIN A ISOLATES OF Justicia gendarussa Burm.f. LEAF IN REVERSE TRANSCRIPTASE IN HIBITION OF HIV TYPE I INVITRO E. W., Bambang Prajogo; Widiyanti, Prihartini; Nasronudin, Nasronudin; Aksono, Bimo
Indonesian Journal of Tropical and Infectious Disease Vol. 5 No. 5 (2015)
Publisher : Institute of Topical Disease Universitas Airlangga

Screening has been done to a few extracts from the leaves Justicia gendarussa Burm.f to see the growth rate of the virus from the blood plasma of HIV patients at Dr Soetomo Hospital. It is known that J. gendarussa leaf extract inhibits HIV type 1 reverse transcriptase. In addition, its main content is gendarussin A, besides gendarussin B, JGF1, JGF2 and JGF3, which have just identified. At the beginning, extraction and fractionation were performed with 3 models that highlight the absolute methanol, 70% methanol and 70% ethanol with the release of alkaloids. Furthermore, samples of each fraction were incubated in plasma of HIV patients with a titer of 3.6 10^6 copies for 1 h in concentrations of 1.64 ppm, 4.1 ppm, 8.2 ppm, 16.4 ppm and 41.0 ppm. After incubation, examination was performed by using Nucli sens a machine, which is a combination of PCR and Elisa, thus avoiding direct contact with the highly pathogenic virus. The result showed that the activity sequence from the most potential to the weak, among others, was 1.64 ppm >4.1 ppm > 8.2 ppm > 16.4 ppm > 41.0 ppm, each with barriers value of 0.62 10^6, 1.4 10^6, 1.6 10^6, 2.4 10 cells/ml. In conclusion, highest anti-HIV activity comes from the concentration of gendarussin A isolate at 1.64 ppm. Furthermore, after linearregression of y = -3.063 x + 81.37 was done, the IC50 of 10.24 ppm was obtained.

EFFECT OF FREE ALKALOID AND NON-FREE ALKALOID ETHANOL 70% EXTRACT OF JUSTICIA GENDARUSSA BURM F. LEAVES AGAINST REVERSE TRANSCRIPTASE HIV ENZYME IN VITRO AND CHEMICAL COMPOUND ANALYSIS Prajogo, Bambang; Widiyanti, Prihartini; Riza, Hafrizal
Indonesian Journal of Tropical and Infectious Disease Vol. 6 No. 1 (2016)
Publisher : Institute of Topical Disease Universitas Airlangga

HIV-AIDS is a global problem and the deadliest disease in the world. One of HIV and AIDS prevention strategy can be done with traditional medicine research program from natural resource that has anti-HIV AIDS activity. It has been found that 70% ethanol extract of Justicia gendarussa Burm.f leaves, alkaloid free and alkaloid non-free, has a strong inhibitory activity against HIV reverse transcriptase enzyme, as an effort to find a solution in the face of HIV AIDS prevalence that is still high with problem of HIV-AIDS treatment such as side effects and resistances. Justicia gendarussa had already known for having an effect anti-HIV and therefore we were looking at the mechanism of inhibition of HIV Reverse Transcriptase enzyme. Both types of extracts were tested in vitro using ELISA technique and analysed chemical content of Gendarusin A as anti-HIV using high performance liquid chromatography. ELISA test results obtained percent inhibition, respectively for 254.2, 254.2, 235.6, and 279.7 for the concentration of 5 ppm, 10 ppm, 15 ppm and 20 ppm of free alkaloid extract and 169.0, 164.0, 130.5 and 369.5 for the concentration of 5 ppm, 10 ppm, 15 ppm and 20 ppm of non-free-alkaloid extract. The results of high performance liquid chromatography obtained Gendarusin A in the free alkaloid extract at retention time 8.402 minutes and non-free alkaloid extract at retention time 8.381. Therefore, these results concluded that the Justicia gendarussa Burm.f can be a useful resource for the isolation and development of new anti-HIV.

CYTOTOXICITY OF JUSTICIA GENDARUSSA BURM F. LEAF EXTRACTS ON MOLT-4 CELL Widiyanti, Prihartini; Prajogo, Bambang; Hikmawati, Ni Putu Ermi
Indonesian Journal of Tropical and Infectious Disease Vol. 6 No. 1 (2016)
Publisher : Institute of Topical Disease Universitas Airlangga

Justicia gendarussa Burm f. (Acanthaceae) is known for its activity as a male contraceptive and anti-HIV properties. The present study was designed to evaluate extracts of J. gendarussa for cytotoxicity activity against MOLT-4 cells. The cytotoxic activity of the fractionated-extract and 70% ethanol extracts of J. gendarussa leaves on MOLT-4 cells were evaluated using a WST-1 assay. The treatment cells, control cells without treatment and control media were also tested in duplicate. The absorbance was measured at a wavelength of 450 nm using a microplate absorbance reader (Bio-Rad). The average absorbance measures formazan produced by viable cells that metabolize the WST-1 reagent. Then the data was analyzed with regression analysis Microsoft Excel 2007 program to determine the concentration with 50% cell viability (50% Cytotoxicity Concentration, CC50). The CC50 values of the fractionated-extract and 70% ethanol extract of J. gendarussa leaves were 94 Î¼g/ml and 78 Î¼g/ml, respectively. The cytotoxicity of fractionated-extract and 70% ethanol extract of J. gendarussa leaves were not significantly different (p > 0.05). It can be concluded that the fractionated-extract and 70% ethanol extract of J. gendarussa leaves are not toxic to MOLT-4 cells.

Mini Review: Quality Control Study of Crude Drug of Justicia gendarussa Burm. f. Leaves as Male Contraceptive Ummah, Rokhmatul; Wardoyo, Bambang Prajogo Eko; Widyowati, Retno
MPI (Media Pharmaceutica Indonesiana) Vol. 4 No. 1 (2022): JUNE
Publisher : Fakultas Farmasi, Universitas Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24123/mpi.v4i1.4838

Justicia gendarussa Burm. f. (Gendarussa) leaves contain gendarusin A, which is a potential antifertility agent. Preclinical researches (in vitro, in vivo, and toxicity) and clinical trials have been carried out proving that Gendarussa leaves are safe to be used as a male contraceptive. Quality control of medicine must be carried out from the beginning when selecting crude drugs until the production process. Quality control of the crude drug is needed to maintain the quality, safety, and efficacy of its crude drug. That standardization has been done in Kediri, Mojokerto, and Ponorogo. The research output data used were specific and nonspecific parameters (macroscopic and microscopic assay, determination of ash content, compound levels, etc.). The quality of crude drugs is different in each region. Crude drug from Mojokerto is better than crude drug from Kediri and Ponorogo. This is influenced by environmental difference factors on how plants grow. This review describes the differences in the quality control results of crude drug of Gendarussa from several areas. Hence, in the future, the quality of this plant can be guaranteed to be used as a contraceptive drug.

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