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Khoirul Ngibad
Universitas Maarif Hasyim Latif
Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Economics, Econometrics & Finance Education Health Professions Immunology & microbiology Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Public Health Social Sciences Other

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In Vitro Antioxidant Activity, Flavonoid and Phenolic Contents of 70% Methanol Extract from Purple and Yellow Passion Fruit Peel Khoirul Ngibad; Siti Nur Husnul Yusmiati; Setyo Dwi Santoso; Firda Inayah; Rizky Dwi Nur Faizi
Indonesian Journal of Chemistry and Environment Vol 6, No 2 (2023): DECEMBER 2023
Publisher : Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/ijoce.v6i2.57725

Abstract

Peel of purple and yellow passion fruit has the potential to be an antioxidant. The flavonoid and total phenol compound groups are two examples of natural antioxidant compounds that can potentially be antioxidants. Many studies have used 70% methanol solvent to extract flavonoids and total phenolics from plants. The aim of this study is to analyze the potential of 70% methanol extract from the peel of purple and yellow passion fruit as an in vitro antioxidant using the DPPH method, total flavonoid, and phenolic levels. The passion fruit peel powder was macerated with 70% methanol for four days. The total flavonoid contents were determined using quercetin, and total phenols were determined using gallic acid. The 2.2-diphenyl-1-picrylhydrazyl (DPPH) was used for in vitro antioxidant activity by determining the percent inhibition of DPPH radicals. The flavonoid content in the 70% methanol extract of purple and yellow passion fruit peel is 1.2824 and 1.9775 mg QE/g extract, respectively. In contrast, the total phenolic content in 70% methanol extract of purple and yellow passion fruit peel is 15.8 and 3.6 mg GAE/g extract, respectively. The 70% methanol extract of purple passion fruit peel at a concentration of 80 mg/L resulted in a more significant percent inhibition of DPPH free radicals (72.80 %) than yellow passion fruit (64.91 %). In conclusion, the results suggest that 70 % methanol extract from purple and yellow passion fruit peel can decrease free radicals and be a natural antioxidant.Keyword: Antioxidant activity, Flavonoid, Methanol extract, Passion fruit peel, Total phenol
Co-Authors Adrian Wahyu Adi Saputra Afisa Asmaanis Ahmad Rizal Tohari Ajeng Setya Apriolivia Alfin Setya Ningrum Allifa Hanna Rizki Anita Firdaus Anton Yuntarso Asma'ul Fadlillah Brilliant Margalin Cakra Haryo Wibowo Dendi Rinaldi Dheasy Herawati Dwi Nur Qoiriyah Erfin Essy Oktarina Fahlia Nirmala Firda Inayah Halik Wijaya Ika Widya Ardhyani Khairil Anam Kholifatuz Zumaroh Khotmil Ula M Farkhan Moch Lazuardi Imani Moh. Mustofa Moh. Zakaria Zakki Mouza Sutra Karmelia Putri Vito Muh Sungging Pradana Muhamad Agung Laksono Muji Harsini Mukti Ali Nevita Lexia Nilam Maghfiroh Novia Afriani Donatomo Putri Alifia Arofah Rafika Destiana Wardani Raymond F Runtu Reza Ichsan Mashuda Risma Wanda Putri Rizky Dwi Nur Faizi Sembilu, Nambi Setyo Dwi Santoso Sholikudin Sholikudin Siti Nur Husnul Yusmiati Subagyo Subagyo Sulthan Al Akbar Titik Sundari Vega Permadani Veronica Nindi Rihi Violya Desta Prananda Wahyu Adi Setiawan Wahyu Ningsih Wildan Sam Aden Yosi Nur Susilawati Yusanti Ervina Zelika Nur Jannah