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Journal : HAYATI Journal of Biosciences

Zingiber officinale, Piper retrofractum and Combination Induced Apoptosis and p53 Expression in Myeloma and WiDr Cell Lines HENY EKOWATI; ANISYAH ACHMAD; EKA PRASASTI; HENDRI WASITO; KADEK SRI; ZULIA HIDAYATI; TUTI EKASARI
HAYATI Journal of Biosciences Vol. 19 No. 3 (2012): September 2012
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (198.511 KB) | DOI: 10.4308/hjb.19.3.137

Abstract

In previous studies, Zingiber officinale, Piper retrofractum, and the combination showed cytotoxic activity, induced apoptosis, and p53 expression of HeLa, T47D, and MCF-7 cell lines. This study was conducted to investigate the cytotoxic and apoptotic activity of Zingiber officinale (ZO), Piper retrofractum (PR), and the combination as well as their effect to p53 expression on Myeloma and WiDr cells. The powder of ZO, PR, and ZO + PR combination (1:1) were macerated with 96% ethanol for 3 x 24 hours. MTT cytotoxic assay was performed on Myeloma and WiDr cell lines. Apoptotic cells were stained with ethidium bromide and acridine orange. Imunohistochemical expression of p53 was examined on Myeloma and WiDr cell lines. Doxorubicin was used as positive control in all assays. Results showed that ZO, PR, and ZO + PR combination had cytotoxic activity on Myeloma cells with IC50 of 28, 36, and 55 mg/ml respectively and WiDr cell lines with IC50 of 74, 158, and 64 mg/ml respectively, induced apoptotic activity, and increased p53 expression on Myeloma and WiDr cells. These results suggest that ZO, PR, and their combination induced Myeloma and WiDr cells in apoptosis through p53 expression.
Diterpene Lactones from Andrographis paniculata and In Vitro Studies on α-Amylase and α-Glucosidase Inhibitory Properties Rachmani, Eka Prasasti Nur; Rehana; Lestari, Puji; Fajriah, Sofa
HAYATI Journal of Biosciences Vol. 32 No. 5 (2025): September 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.5.1295-1302

Abstract

Plants contain chemical compounds that have medicinal properties. Andrographis paniculata (bitter herb) is commonly used as a traditional medicine, including treating hyperglycemia. A. paniculata was known to contain major compounds, terpenoids, and flavonoids. The phytochemical investigation aimed to isolate and identify chemical compounds from the aerial part of the bitter herb and then evaluate the antihyperglycemic properties. A. paniculata was extracted using 96% ethanol. The extract was partitioned with three organic solvents successively to obtain the chloroform fraction, ethyl acetate fraction, and methanol fraction. Isolation was carried out on the ethyl acetate fraction using column chromatography with a silica stationary phase and an organic solvent mobile phase in various ratios. The isolate in the form of white powder was found in subfraction E8 with the mobile phase chloroform-ethyl acetate (1-2). The structure of the isolated compounds was characterized using spectroscopic methods, including 1D NMR (1H and 13C), 2D NMR (HSQC and HMBC), and LCMS. The isolated compounds were evaluated in vitro with α-amylase and α-glucosidase tests. The results of the data analysis indicated the identification of 14-deoxy-11,12-didehydroandrographolide and 14-deoxyandrographolide as the isolated compounds. The isolated compounds showed α-amylase inhibitory activity with an inhibitory concentration (IC50) of 167.31±4.92 μg/ml and α-glucosidase inhibitory activity with an IC50 of 267.10±6.72 μg/ml. The results of this study indicate that paniculata contains diterpene lactones, which are active in hyperglycemia therapy.