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All Journal Jurnal Teknologi Dan Industri Pangan Jurnal Teknologi Industri Pertanian Microbiology Indonesia BIOTROPIA - The Southeast Asian Journal of Tropical Biology Indonesian Journal of Agricultural Science Jurnal AgroBiogen Jurnal Penelitian Pascapanen Pertanian Pengembangan Inovasi Pertanian Buletin Teknologi Pasca Panen agriTECH PROSIDING SEMINAR KIMIA BERITA BIOLOGI Informatika Pertanian Indonesian Journal of Chemistry Menara Perkebunan Pharmaceutical Sciences and Research (PSR) Amwaluna: Jurnal Ekonomi dan Keuangan Syariah
Nur Richana
Universitas Ibn Khaldun
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Economics, Econometrics & Finance Engineering Immunology & microbiology Industrial & Manufacturing Engineering Medicine & Pharmacology Veterinary

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Journal : Jurnal AgroBiogen

 1 
Purifikasi dan Karakterisasi α-amilase Termostabil dari Bacillus stearothermophilus TII-12 Lestari, Puji; Richana, Nur; Darwis, Abdul Aziz; Syamsu, Khaswar; Murdiyatmo, Untung
Jurnal AgroBiogen Vol 7, No 1 (2011): April
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v7n1.2011.p56-62

Abstract

Purification and Characterization of Thermostableα-amylase from Bacillus stearothermophilus TII-12. PujiLestari, Nur Richana, Abdul A. Darwis, Khaswar Syamsu,and Untung Murdiyatmo. Thermostable α-amylase is apotential enzyme employed in the starch processing andwidely used in food industries, but this enzyme is stillimported. The local enzyme production would be moreeconomist and useful for its broad applications. Here wereport α-amylase from indigenous bacteria TII-12 which waspurified and characterized, as well as analyzed its hydrolysisproduct on cassava starch. The enzyme of Bacillusstearothermophilus TII-12 partially purified by ultrafiltration,acetone precipitation and gel filtration (Sephadex G-100)showed the reduced total activity, total protein and yield, butincreased the specific activity. The enzyme had a Km of 1,06mg/ml and Vmax of 1,21 mol/min, with optimal activity at pH 7and 90oC. An apparent molecular mass was of 192.932,8Dalton, as estimated by Native-Polyacrylamide Agarose Gelelectrophoresis. Its activity was inhibited by the divalentcation chelator such as EDTA and CuSO4 but activated bycalcium ion. Hydrolysis products of this enzyme on cassavastarch were glucose, dextrin, maltose and oligosaccharides.After 24 hours of hydrolysis, the concentration of glucoseand maltose reached 51.970 and 10.090 ppm, respectively.The thermostable α-amylase of TII-12 is an endo-α-amylaseand prospective to be applied on starch liquefaction withhigh temperature process.
Isolasi Identifikasi Bakteri Penghasil Xilanase serta Karakterisasi Enzimnya Nur Richana; Tun T. Irawadi; Anwar Nur; Khaswar Syamsu
Jurnal AgroBiogen Vol 4, No 1 (2008): April
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v4n1.2008.p24-34

Abstract

Xylanase is an extracellular enzyme produced bymicroorganisms. This enzyme is able to hydrolise xylane(hemicellulose) to produce xylooligosaccharide and xylose.Thermoalkaliphilic xylanase is an agent that can be used asa substitute in the pulp whitening process instead of chlorine.A study was done to isolate, identificate of bacteria andcharacterize xylanase. The isolation of xylanase producingbacteria has been done from soil and waste of starch industry.Colonies which produced clearing zone were presumedas xylanolytic bacteria and chosen for further screening.Identification of potential isolate in xylanase production wasdone using 16S ribosomal RNA sequencing. Isolate Bacilluspumilus RXA-III5 originated from lime or alkaline soil wasmore potential isolate in xylanase production than other 24isolates. Precipitation of xylanase, that was done usingammonium sulphate followed by dialyzes produced xylanaseof a higher specific activity (267.1 U.mg-1) than that usingacetone (131.1 U.mg-1) and ethanol (186.65 U.mg-1). Xylanasewas done at purification produced three fractions of xylanase.Xylanase characteristics consist of pH and temperature(9 and 50oC), Km and Vmaks value 6 mg.ml-1 and 0.2mol.minute-1, respectively. The Fe2+ was the strongest activetorand Mg2+ was the strongest inhibitor activity. This enzymewas detected as a cellulose-free xylanase. Xylanase is aprospective agent for bio-bleaching of paper.
Co-Authors A.A. Ketut Agung Cahyawan W Abdullah bin Arif Abdullah Bin Arif Achmad Zainudin Agus Budiyanto Agus Budiyanto Anja Meryandini Anwar Nur Anwar Nur Arif SP.MSi, Abdullah Bin Arif, Abdullah bin Budiyanti, Agus Budiyanto, Agus Christina Winarti Darwis, Abdul Aziz Dedi Fardiaz Diyono, Wahyu Diyono, Wahyu Djumali Mangunwidjaja Djumali Mangunwidjaja Dwi Setyaningsih Eka Ruriani EKA RURIANI Endang Prangdimurti Endang S Rahayu Endang S Rahayu Eni Harmayani Eni Harmayani Enrico Syaefullah Erliza Noor Evi Savitri Iriani Faizal Gayang Firda DIMAWARNITA Hanny Hafiar Hayuningtyas, Maulida I Ketut Suada I Wayan Arnata Illah Sailah Irfan Syauqi Beik Irinne D.P Khaswar Syamsu Lestari, Puji M. ANWAR NUR MARIA BINTANG Maulida Hayuningtyas Munarso, S. Joni Murdiyatmo, Untung nFN Suliantari nFN Widaningrum Nurhayati, Immas Puji Lestari Puji Lestari PUJI LESTARI Purwiyatno Hariyadi Rahmawati Rahmawati Ratih Dewanti -Hariyadi Ratnaningsih Ratnaningsih Ribowo, Sugeng Rina Masriani Rosmimik Rosmimik Rosmimik, Rosmimik Sakae Suzuki SOFIYANTO, M. EDY Sri Laksmi Suryaatmadja Sri Usmiati Suharyanto Titi C Sunarti Titi C. Sunarti Titi Candra Sunarti Titi Chandra Sunarti Tri Marwati Tri Marwati Tri Marwati Tri Panji Tun T. Irawadi Tun Tedja Irawadi Wahyu Diyono Wahyu Diyono Yandra Arkenan Yoshiharu Fujii