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All Journal HAYATI Journal of Biosciences Jurnal Fitopatologi Indonesia BIOTROPIA - The Southeast Asian Journal of Tropical Biology Agrokompleks

Sari Nurulita

Institut Pertanian Bogor

Author-ID : 335888

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Immunology & microbiology Medicine & Pharmacology Veterinary

Published : 11 Documents Claim Missing Document

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Infeksi Papaya ringspot virus pada Tanaman Pepaya di Provinsi Nanggroe Aceh Darussalam Sri Hendrastuti Hidayat; Sari Nurulita; Suryo Wiyono
Jurnal Fitopatologi Indonesia Vol 8 No 6 (2012)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Severe mosaic symptoms was observed on papaya plants in Meusa village, Kutablang sub district, Bireun district and Lambaro Teunom village, Lembah Seulawah sub district, Aceh Besar district. Systemic mosaic was found in leaves, stem, twig, and fruit. Reverse transcription-polymerase chain reaction (RT-PCR) was conducted to detect the virus causing mosaic symptoms using universal primer for Potyvirus. Amplification of 320 bp DNA fragment was successfully obtained from leaves and fruits bearing severe mosaic symptoms. Nucleotide sequencing was proceeded using the DNA amplicon as the template. Further sequence analysis indicated that Potyvirus isolates from papaya in Aceh show the highest homology (92.7% to 94.7%) with Papaya ringspot virus (PRSV) from Philippines, Thailand, and Indonesia. This is the first report on PRSV infection on papaya in Nanggroe Aceh Darussalam province. Key words: Potyvirus, RT-PCR, sequencing

Identifikasi Tomato infectious chlorosis virus dan Tomato chlorosis virus melalui Reverse Transcription Polymerase Chain Reaction dan Analisis Sikuen Nukleotida Sari Nurulita; Gede Suastika
Jurnal Fitopatologi Indonesia Vol 9 No 4 (2013)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Tomato chlorosis disease was found more frequent on production area in West Java recently. Observation in the field showed typical symptoms of Tomato infectious chlorosis virus (TICV) and Tomato chlorosis virus (ToCV). The objective of this research is to identify TICV and ToCV associated with chlorosis disease on tomato plants in West Java (Cipanas, Lembang and Garut) using reverse transcription polymerase chain reaction (RT-PCR) method and nucleotide sequence analysis. DNA target of 417 bp and 360 bp was successfully amplified only from Cipanas sample using specific primers, TICV-CF/TICV-CR and ToCV-CF/ToCV-CR, respectively. Sequence analysis confirmed that chlorosis disease in Cipanas was associated with TICV and ToCV infection. Nucleotide sequence and phylogenetic analysis showed that TICV from Cipanas has high homology to and belongs to the same group with TICV from Japan and Spain; whereas ToCV from Cipanas has high homology to and belongs to the same group with ToCV from America.

Kloning Gen Transcriptional Activator Protein dan Replication-Associated Protein dari Squash leaf curl China virus Penyebab Penyakit Daun Kuning pada Tanaman Mentimun Ni Nengah Putri Adnyani; I Dewa Made Putra Wiratama; I Gede Rai Maya Temaja; Sari Nurulita; Gede Suastika
Jurnal Fitopatologi Indonesia Vol. 14 No. 1 (2018)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Yellow disease on Cucurbitaceae caused by Squash leaf curl China virus (SLCCNV; Begomovirus) is an important disease in many countries. Typical yellow leaf curl symptoms was found on cucumber in Tabanan, Bali. The objective of this research is to clone Transcriptional Activator Protein (TrAp) and Replication-Associated Protein (Rep) of SLCCNV Indonesian isolate. Universal primer for Begomovirus, i.e. SPG1/SPG2 was used to amplify DNA fragment of Begomovirus which cover the TrAp and Rep gene. The amplicon of ± 912 bp in size was then cloned into pTZ57R/T plasmid vector by TA-cloning method to obtain plasmid recombinant pTZ-SLCCNV. The plasmid recombinant was successfully confirmed by PCR colony and DNA sequencing. The nucleotide sequences analysis of plasmid recombinant showed highest homology (98%) of the corresponding gene to pumpkin isolate of SLCCNV from Malaysia.

Pengembangan Deteksi Spesifik untuk Mungbean Yellow Mosaic India Virus yang Menginfeksi Kacang Panjang di Jawa, Indonesia Nurulita, Sari; Mutaqin, Kikin; Hidayat, Sri
Jurnal Fitopatologi Indonesia Vol 19 No 2 (2023): Maret 2023
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.19.2.83-88

Yellow mosaic disease was reported for the first time in Indonesia in 2008. Its infection on yard long bean caused significant yield loss. Mungbean yellow mosaic India virus (MYMIV), member of genus Begomovirus was identified as the main causal agent. Specific and accurate detection is important for disease monitoring as part of disease management strategy. The aim of this study was to construct specific primer pairs for quick and robust detection of MYMIV using polymerase chain reaction method. A pair of primers MY1/MY2 was designed in this study to amplify part of MYMIV coat protein. In silico and in vitro test showed that MY1/MY2 primers specifically amplified MYMIV.

First Report of Celery mosaic virus Infecting Celery (Apium graveolens) in Indonesia: Laporan Pertama Celery mosaic virus yang Menginfeksi Seledri (Apium graveolens) di Indonesia Makyorukty, Dhayanti; Damaiyanti, Kartika Catur; Sianipar, Theresia Aprilia; Nurulita, Sari
Jurnal Fitopatologi Indonesia Vol. 20 No. 3 (2024): Mei 2024
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.20.3.109-114

Celery mosaic virus (CeMV), anggota dari genus Potyvirus dilaporkan pertama kali di Indonesia, menginfeksi seledri (Apium graveolens) pada lahan pertanaman sayuran di Berastagi, Provinsi Sumatera Utara. Tanaman tersebut memiliki gejala mosaik dan pemucatan tulang daun yang merupakan ciri khas infeksi CeMV. Infeksi virus dikonfirmasi dengan RT-PCR menggunakan degenerate primer potyvirus yang mengamplifikasi genom virus, yaitu sebagian protein selubung dan 3’-UTR. Analisis pohon filogenetik menempatkan isolat CeMV Indonesia dalam satu klaster terpisah dalam kelompok CeMV dan memiliki kemiripan nukleotida sebesar 96.5%–96.7% dengan isolat contoh CeMV.

Identification of Garlic Viruses Associated with Seed Bulbs and Consumption Bulbs from Several Locations in Indonesia Nurulita, Sari; Mawarni, Sofi; Hidayat, Sri Hendrastuti
HAYATI Journal of Biosciences Vol. 31 No. 4 (2024): July 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.4.733-743

Virus infection is one of the major constraints in garlic production since the viruses are readily accumulated on vegetative propagation material (bulbs). This research aimed to detect garlic common latent virus (GCLV), shallot latent virus (SLV), onion yellow dwarf virus (OYDV), and leek yellow stripe virus (LYSV) infecting local garlic as seed bulb and imported garlic as consumption bulb. Seed bulb samples were obtained from seed breeders in several garlic growing centers in Indonesia. In contrast, consumption bulb samples were obtained from plant quarantine warehouses and three local markets in Bogor. Some bulb samples were used for morphological observations, and some were germinated in the laboratory until the leaves emerged. Leaves were collected for virus detection by RT-PCR using specific primers for GCLV, SLV, OYDV, and LYSV. Seed and consumption bulbs have differences in their morphological characteristics, especially in the type of neck hardness and the size of the bulb diameter. OYDV and LYSV infections were successfully detected in seed and consumption bulbs, while SLV was only found in consumption bulbs. Nucleotide sequence analysis showed that SLV from consumption bulbs formed one group, GCLV from seed bulbs formed one group, while OYDV and LYSV from seed and consumption bulbs were in different groups, indicating that the viruses came from different strains. Further research through high-throughput detection methods and providing virus-free planting material are needed to anticipate the spread of new strains of garlic viruses in Indonesia.

Molecular Evidence Points to Strong Resemblance in the Parasitoid Species of Rice and Cogongrass Gall Midges, Platygaster spp. (Hymenoptera: Platygasteridae) Hidayat, Purnama; Lestari, Yuliana Ayu; Nurulita, Sari
HAYATI Journal of Biosciences Vol. 32 No. 3 (2025): May 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.3.683-692

The rice gall midge, Orseolia oryzae, and the cogongrass gall midge, O. javanica, cause gall formation on rice and cogongrass (alang-alang) (Imperata cylindrica). Two different species parasitize these two gall midges but closely related platygasterids, Platygaster oryzae on the rice gall midge and P. orseoliae on the cogongrass gall midge. Both the gall midges and their parasitoids are often found in the adjacent area, raising a question about the relationship between the two gall midges and their parasitoids. This research aims to study the molecular identity of the rice and cogongrass gall midges, along with their platygasterid parasitoids, based on partial sequences of the mtCOI gene. Samples were collected from rice and cogongrass in the adjacent area in Cianjur, West Java Province, and a rice field with no cogongrass in Bogor, West Java Province. Successful DNA amplification was achieved using universal primers for mtCOI. Nucleotide sequencing analysis revealed that the rice gall from Bogor and Cianjur shared 100% similarity and 93.2-99.3% similarity with the rice gall from other countries. Notably, the parasitoids P. oryzae collected from rice in Bogor and Cianjur shared 97.2% similarity with P. orseoliae collected from cogongrass in Cianjur. These findings suggest that the platygaster parasitoids associated with the rice gall and the cogongrass gall midges are identical, serving as potential natural enemies for both pests. This study represents the first molecular identification report of rice and cogongrass gall midges and their platygasterid parasitoids from Java Island, Indonesia.

Identifikasi kutu daun yang berasosiasi dengan tanaman jeruk Syarni, Elisa; Taufik, Muhammad Taufik; Yusuf, Dewi Nurhayati; Nurulita, Sari; Miftakhurohmah, Miftakhurohmah; Botek, Muhammad; HS, Gusnawaty; Hikamwaty, Aisyah
Agrokompleks Vol 25 No 2 (2025): Vol 25 No 2 (2025): Agrokompleks Edisi Juli
Publisher : Politeknik Pertanian Negeri Pangkajene Kepulauan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51978/japp.v25i2.918

Aphids are polyphagous insects commonly found on various plants, including citrus plants. The association between aphids and citrus plants plays a role as pests and vectors of citrus plant diseases, particularly Citrus tristeza virus (CTV). Various aphid species have been reported as CTV vectors, including Aphis gossypii, Toxoptera aurantii, and T. citricidus. So far, no information has been on aphid species associated with citrus plants in Southeast Sulawesi. Therefore, this study aimed to identify aphid species associated with citrus plants. The research method used was observation and collection of aphids found colonizing citrus plants. The collected aphids were then brought to the laboratory for detection using the polymerase chain reaction (PCR) technique. The study results showed that the aphid species associated with citrus plants was Aphis citricidus. The PCR technique successfully detected the genome of aphid vector samples from Konawe and South Konawe (700 bp). This information is a fundamental basis for controlling CTV in mainland Southeast Sulawesi.

Identification of Polerovirus Infecting Chili Pepper in Java: Identifikasi Polerovirus yang Menginfeksi Tanaman Cabai di Jawa Saputra, Andri; Giyanto; Nurulita, Sari; Hidayat, Sri Hendrastuti
Jurnal Fitopatologi Indonesia Vol. 21 No. 1 (2025): Maret 2025 - IN PROGRESS
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.21.1.1-6

Identifikasi Polerovirus yang Menginfeksi Tanaman Cabai di Jawa Infeksi polerovirus pada tanaman cabai semakin banyak dilaporkan dari berbagai bagian dunia termasuk di Indonesia. Infeksi Pepper vein yellow virus (PeVYV) pada tanaman cabai di Bali, Jawa, dan Aceh telah dilaporkan pada kurun waktu 2012 sampai 2020. Belum lama ini gejala infeksi polerovirus berupa daun kuning dengan penebalan tulang daun ditemukan pada pertanaman cabai di wilayah Jawa Tengah, yaitu di Brebes, Magelang dan Kulon Progo. Deteksi molekuler dengan metode RT-PCR menggunakan primer universal polerovirus O3R/PL4F yang dilanjutkan dengan analisis sekuens membuktikan adanya infeksi PeVYV dari sampel asal Magelang dan Kulon Progo. Identifikasi lebih lanjut menunjukkan bahwa isolat PeVYV tersebut ialah PeVYV-2, PeVYV-6, dan PeVYV-8. Insidensi infeksi PeVYV ini menunjukkan perluasan distribusi Polerovirus di Indonesia.

Status Terkini Penyakit Tristeza Pada Tanaman Jeruk di Sulawesi Tenggara: Current Status of Citrus Tristeza Disease in Southeast Sulawesi Taufik, Muhammad; Yusuf, Dewi Nurhayati; Miftahkuhrohmah, Miftahkuhrohmah; Botek, Muhammad; Gusnawaty HS, Gusnawaty HS; Syarni, Elisa; Nurulita, Sari
Jurnal Fitopatologi Indonesia Vol. 21 No. 1 (2025): Maret 2025 - IN PROGRESS
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.21.1.28-37

Penyakit tristeza yang disebabkan oleh citrus tristeza virus (CTV, genus Closterovirus, famili Closteroviridae) merupakan salah satu penyakit penting pada tanaman jeruk di dunia, termasuk di Indonesia. Hingga kini, informasi dan studi terkait CTV di Indonesia masih terbatas. Penelitian ini bertujuan untuk mengetahui status terkini infeksi CTV di Provinsi Sulawesi Tenggara. Survei dan pengambilan sampel dilakukan secara purposive sampling di empat kabupaten. Deteksi dan identifikasi virus dilakukan dengan teknik double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA), reverse transcription polymerase chain reaction (RT-PCR), dan analisis perunutan nukleotida. Hasil DAS-ELISA menunjukkan bahwa semua sampel dengan berbagai variasi gejala dari empat kabupaten terkonfirmasi positif terinfeksi CTV dengan nilai titer virus yang beragam. RT-PCR dengan primer spesifik berhasil mengonfirmasi keberadaan CTV dari semua sampel dengan teramplifikasinya DNA berukuran 630 pb. Analisis sekuen dan filogeni menunjukkan bahwa isolat CTV asal Sulawesi Tenggara memiliki kemiripan tertinggi (97.9%) dengan isolat CTV asal India (MT498411.1) dan mengelompok satu grup dengan isolat asal India (MT498411.1), Jepang (U56902.1), dan Cina (MZ692538.1). Hasil penelitian ini mengonfirmasi penyebaran CTV pada empat kabupaten di Sulawesi Tenggara. Data molekuler diharapkan menjadi basis data untuk studi epidemiologi molekuler dan strategi pengendalian penyakit tristeza di Indonesia.

Co-Authors Botek, Muhammad Damaiyanti, Kartika Catur Gede Suastika Giyanto GUSNAWATY HS, GUSNAWATY Hidayat, Sri Hikamwaty, Aisyah I Dewa Made Putra Wiratama I G. R. M. TEMAJA John Thomas, John Kikin H Mutaqin Lestari, Yuliana Ayu Makyorukty, Dhayanti Mawarni, Sofi Miftahkuhrohmah, Miftahkuhrohmah MIFTAKHUROHMAH MIFTAKHUROHMAH, MIFTAKHUROHMAH MUHAMMAD TAUFIK Ni Nengah Putri Adnyani PURNAMA HIDAYAT RR. Ella Evrita Hestiandari Safinah Surya Hakim, Safinah Surya Sianipar, Theresia Aprilia SRI HENDRASTUTI HIDAYAT Suryo Wiyono Syarni, Elisa Taufik, Muhammad Taufik Tri Wira Yuwati, Tri Wira Yusuf, Dewi Nurhayati

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