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All Journal Jurnal Penelitian Tanaman Industri JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Perspektif, Review Penelitian Tanaman Industri Buletin Penelitian Tanaman Rempah dan Obat Agrokompleks
MIFTAKHUROHMAH MIFTAKHUROHMAH, MIFTAKHUROHMAH
Balai Penelitian Tanaman Obat dan Aromatik Jl. Tentara Pelajar No. 3A, Bogor
Agriculture, Biological Sciences & Forestry Industrial & Manufacturing Engineering

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DETEKSI SECARA SEROLOGI DAN MOLEKULER BEBERAPA JENIS VIRUS YANG BERASOSIASI DENGAN PENYAKIT MOSAIK TANAMAN NILAM (Pogostemon cablin Benth) MIFTAKHUROHMAH, MIFTAKHUROHMAH; SUASTIKA, GEDE; DAMAYANTI, TRI ASMIRA
853-8212
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

ABSTRAKPenyakit mosaik pada tanaman nilam disebabkan oleh beberapajenis virus, yaitu Potyvirus, Potexvirus, Cucumber mosaic virus (CMV),dan Broad bean wilt virus 2 (BBWV2). Penelitian ini bertujuan untukmengidentifikasi secara serologi dan molekuler virus-virus yangberasosiasi dengan gejala mosaik pada nilam di KP. Manoko, KP. Cicurugdan lahan petani di Cijeruk. Sampel daun nilam baik yang menunjukkangejala mosaik atau pun tidak diambil dari setiap lokasi penanamanmasing–masing sebanyak 30 sampel. Kejadian penyakit ditentukan melaluideteksi serologi dengan Direct-ELISA dan Indirect-ELISA terhadap sampelmenggunakan empat antiserum, yaitu CMV, Cymbidium mosaic virus(CymMV), Potyvirus, dan BBWV2. Deteksi molekuler dengan RT-PCRdilakukan untuk mengonfirmasi virus baru yang ditemukan. Hasilpenelitian menunjukkan bahwa gejala infeksi virus yang ditemukan padanilam bervariasi, yaitu mosaik lemah, mosaik kuning hijau, mosaik denganpenebalan, mosaik dengan malformasi daun, dan bintik kuning. Secaraserologi, kejadian virus pada setiap kebun bervariasi. Di KP Manoko,Potyvirus dan BBWV2 lebih dominan (100%) dibandingkan CymMV. DiKP Cicurug, kejadian Potyvirus dan CMV terlihat lebih dominan (83,3 dan80%) dibandingkan BBWV2 dan CymMV, sedangkan di Cijeruk, BBWV2lebih dominan (90%) dari Potyvirus (50%) dan CMV (13,3%). Hasil RT-PCR dengan primer degenerate BBWV, diidentifikasi BBWV2 padasampel daun nilam dari Manoko, Cicurug, dan Cijeruk, sedangkan denganprimer general Potexvirus, diidentifikasi CymMV hanya dari sampel daunnilam dari asal Manoko. Hasil penelitian ini merupakan laporan pertamatentang BBWV2 dan CymMV pada tanaman nilam di Jawa Barat yangmengindikasikan bahwa virus merupakan kendala utama pada perbenihannilam yang harus segera diatasi.Kata kunci: BBWV2, CymMV, mosaik, Pogostemon cablin Benth, PCRABSTRACTMosaic symptoms on patchouli plant are associated with severalviruses, i.e. Potyvirus, Potexvirus, CMV, and BBWV2. The objective ofthe study was to detect virus(es) associated with mosaic symptoms onpatchouli at the the patchouli seed nurseries, in Manoko, Cicurug, andCijeruk. Thirty leaf samples either showing typical symptomatic mosaic orasymptomatic were taken from each location. Serological testing byDirect-ELISA and Indirect-ELISA using four antisera namely CMV,Cymbidium mosaic virus (CymMV), Potyvirus, and BBWV2 was carriedout to test the incidence of each virus. Molecular detection by RT-PCR wasperformed to confirm the new virus(es). The results showed that symptomsof virus infection were found vary, i.e. weak mosaic, green yellow mosaic,mosaic with thickening, mosaic with leaf malformations, and yellow spot.Based on the serological detection, virus(es) incidence varied at each seednurseries. In Manoko, Potyvirus, and BBWV2 were more dominant(100%) compared with CymMV. In Cicurug, Potyvirus and CMV weremore dominat (83.3 and 80%) compared with BBWV2 and CymMV.While in Cijeruk, BBWV2 was the most dominant (90%) than Potyvirus(50%) and CMV(13.3%). Result of RT-PCR with degenerate primers pairof BBWV was succesfully identified BBWV2 from Manoko, Cicurug, andCijeruk samples, whereas by using Potexvirus general primary, CymMVwas identified only from Manoko samples. BBWV2 and CymMV werefirst reported to infect patchouli in West Java. The result indicate thatvirus(es) are the major constraint on patchouli seed that should bemanaged immediately.Key words: BBWV2, CymMV, mosaic, Pogostemon cablin Benth, PCR
KARAKTERISTIK BIOLOGI DAN MOLEKULER SERTA PENGENDALIAN VIRUS PENYEBAB PENYAKIT KERDIL PADA LADA MIFTAKHUROHMAH, MIFTAKHUROHMAH; BALFAS, RODIAH
Perspektif Vol 13, No 1 (2014): Juni 2014
Publisher : Puslitbang Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/p.v13n1.2014.%p

Abstract

ABSTRAKPiper yellow mottle virus (PYMoV) dan Cucumber mosaik virus  (CMV)  adalah  penyebab  penyakit  kerdil    yang merupakan  satu  penyakit  utama  pada  lada. Perkembangan  penelitian  tentang  penyakit  ini  dan pengendaliannya berlangsung lambat. Hasil penelitian terkini  menyebutkan  bahwa  gejala  penyakit  akibat infeksi tunggal virus adalah berupa klorotik pada daun, sedangkan  infeksi  ganda  menyebabkan  gejala  lebih parah sampai tanaman kerdil. PYMoV yang tergolong ke dalam genus Badnavirus memiliki genom DNA dengan panjang 7.662  nukleotida,  sedangkan  CMV (Cucumovirus) pada lada tergolong ke dalam subgrup I, dekat  dengan  CMV  dari  sirih  dan  Indian  long  pepper.Kedua virus ini menyebar sangat efektif melalui bahantanaman  yang  diperbanyak  secara  vegetatif,  serangga vektor  atau  secara  mekanis.  PYMoV  memiliki  kisaran inang  yang  sempit,  sedangkan  CMV  kisaran  inangnya luas. Kehadiran virus dapat dideteksi secara serologi di Indonesia  dengan  antiserum  BSV.  Secara  molekuler deteksi dilakukan dengan PCR. Pengendalian virus yang disarankan  adalah  secara  preventif,  yaitu  penggunaan bahan  tanaman  bebas/tahan  virus,  pengendalian serangga  vektor  dan  sanitasi  lingkungan.  Perlu dukungan penelitian tentang potensi penularan kedua virus  melalui  biji  dan  vektor  lain,  jenis  tanaman  inang lain  dari  famili  Piperaceae,  mendapatkan  protokol standar  multiplex  PCR,  dan  produksi  bahan  tanaman bebas dan tahan virus.Kata kunci:  PYMoV,  CMV,  penularan,  deteksi, pengendalian  Characteristics and Molecular Biology and Control of Viral Diseases of Dwarf PepperABSTRACTPiper  yellow  mottle  virus  (PYMoV)  and  Cucumber mosaic  virus (CMV)  are  the  causal  agents  of  dwarf disease,  one  of  the  major  diseases  on  pepper.  The development of research on the disease and its control is  slow.  The  results  of  the  current  study  states  that  the symptoms  of  diseases  caused  by  a  single  infectious  virus is a chlorotic on leaves, whereas double infection causes  more  severe  symptoms  until  the  dwarf  plants.PYMoV  belonging  to  the  genus  Badnavirus  have genomic  DNA  with  a  length  of  7,662  nucleotides, whereas CMV (Cucumovirus) on pepper belong to the subgroup  I,  close  to  CMV  of  betel  and  Indian  long pepper.  Both  of  these  viruses  spread  very  effectively through plant material which propagated vegetatively, insect  vectors  or  mechanically.  PYMoV  has  a  narrow host  range,  whereas  CMV  has  a  wide  host  range. The presence of the virus can be detected serologically in  Indonesia  with  antiserum  BSV.  Molecular  detection performed  by  PCR.  Management  strategies  to  control virus  are :  using  virus­free  plant  material,  insect vector  control  and  environmental  sanitation.  The research  support  which  are  required  :  the  potential  of virus  transmission  through  seeds  and  other  vectors, other  host  plants  from  Piperaceae,  get  a  standard multiplex PCR protocol and production of virus­free and  resistant plant material.Keyword :  PYMoV,  CMV,  transmission,  detection, control
PENGARUH BEBERAPA TARAF KONSENTRASI BA TERHADAP MULTIPLIKASI TUNAS CINCAU HITAM (Mesona palustris) IN VITRO Miftakhurohmah, Miftakhurohmah; Syahid, Sitti Fatimah
Buletin Penelitian Tanaman Rempah dan Obat Vol 17, No 1 (2006): BULETIN PENELITIAN TANAMAN REMPAH DAN OBAT
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/bullittro.v17n1.2006.%p

Abstract

Mesona palustris is one of the medi-cinal plant which is potential to be developed. Recently, the agribisnis of this plant commo-dity is considered to be potential. To support  the availability of plant material, propagation by tissue culture technique being a good alternative for mass production. This expe-riment was conducted from January to April 2005 at the Tissue Culture Laboratory of Indonesian Spices and medicinal Crops Research Institute (ISMECRI) in Bogor. The objective of this research was to find out the effect of several concentrations of BA on shoot multiplication of Mesona palustris. The treatments tested were several concentrations of BA e.g. : 0.0 ( control); 0.2 ; 0.4; 0.6; and 0.8 mg/l. Experiment was arranged in a com-pletely randomized design with six replica-tions. The parameters observed were number of shoots, length of shoots, number of leaves, and percentage of rooting shoots, at 3, 5, and 9 week after culture (WAC). The result showed that the use of 0,2 mg/l BA performed the best shoots growth multiplication with a relatively high rate of increased shoots num-ber and percentage of rooting shoots, at 3 to 9 WAC. Abundant shoots number (21.00 shoots), with length of shoots of 5.92 cm, leaves number of 13.00, and percentage of rooting shoots of 83.33% was obtained on MS + BA 0.2 mg/l, 9 WAC. 
Identifikasi kutu daun yang berasosiasi dengan tanaman jeruk Syarni, Elisa; Taufik, Muhammad Taufik; Yusuf, Dewi Nurhayati; Nurulita, Sari; Miftakhurohmah, Miftakhurohmah; Botek, Muhammad; HS, Gusnawaty; Hikamwaty, Aisyah
Agrokompleks Vol 25 No 2 (2025): Vol 25 No 2 (2025): Agrokompleks Edisi Juli
Publisher : Politeknik Pertanian Negeri Pangkajene Kepulauan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51978/japp.v25i2.918

Abstract

Aphids are polyphagous insects commonly found on various plants, including citrus plants. The association between aphids and citrus plants plays a role as pests and vectors of citrus plant diseases, particularly Citrus tristeza virus (CTV). Various aphid species have been reported as CTV vectors, including Aphis gossypii, Toxoptera aurantii, and T. citricidus. So far, no information has been on aphid species associated with citrus plants in Southeast Sulawesi. Therefore, this study aimed to identify aphid species associated with citrus plants. The research method used was observation and collection of aphids found colonizing citrus plants. The collected aphids were then brought to the laboratory for detection using the polymerase chain reaction (PCR) technique. The study results showed that the aphid species associated with citrus plants was Aphis citricidus. The PCR technique successfully detected the genome of aphid vector samples from Konawe and South Konawe (700 bp). This information is a fundamental basis for controlling CTV in mainland Southeast Sulawesi.
Molecular identification and characterization of Maconellicoccus multipori (Takahashi) (Hemiptera: Pseudococcidae) on Piper nigrum L. Mardiningsih, Tri Lestari; Sartiami, Dewi; Miftakhurohmah, Miftakhurohmah; Djiwanti, Setyowati Retno; Rohimatun, Rohimatun; Hardiyanti, Siti; Heryanto, Rubi; Amaria, Widi; Suwarti, Suwarti
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 25 No. 2 (2025): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA: JOURNAL OF TROPICAL PLAN
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.225201-207

Abstract

The mealybug Maconellicoccus multipori (Takahashi) was identified on black pepper (Piper nigrum L.) seedlings in a greenhouse in Bogor, West Java, using both molecular and morphological characterization. Two mealybug isolates were successfully amplified with primer pairs targeting the LCO region of the mitochondrial cytochrome oxidase I (COI) gene, yielding a 491 bp PCR product. The nucleotide sequences of both isolates (GenBank accession numbers LC666906 and LC666907) were identical. Phylogenetic analysis revealed that the Bogor isolates clustered closely with M. multipori populations from China and Thailand, with high sequence homology of 99.30% and 99.10%, respectively. Morphological observations of the adult female specimens further confirmed their identity as M. multipori, based on key diagnostic features including body size, antennal segmentation, cerarii pattern, and distribution of pores and ducts, which correspond to descriptions in established taxonomic keys. This study provides the first molecular characterization of M. multipori in Indonesia. The COI sequence data obtained enhances reference databases for DNA barcoding and strengthens early detection strategies for pest monitoring. These findings are crucial for supporting quarantine inspections, management, and control of M. multipori in black pepper nurseries and preventing its spread to other crops.
Co-Authors Botek, Muhammad DEWI SARTIAMI GEDE SUASTIKA GUSNAWATY HS, GUSNAWATY Heryanto, Rubi Hikamwaty, Aisyah RODIAH BALFAS, RODIAH Rohimatun Rohimatun, Rohimatun Sari Nurulita SETYOWATI RETNO DJIWANTI, SETYOWATI RETNO Siti Hardiyanti Sitti Fatimah Syahid Suwarti Suwarti Syarni, Elisa Taufik, Muhammad Taufik TRI ASMIRA DAMAYANTI Tri Lestari Mardiningsih, Tri Lestari Widi Amaria Yusuf, Dewi Nurhayati