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All Journal Biospecies ILMU KELAUTAN: Indonesian Journal of Marine Sciences Jurnal Natur Indonesia BIOTROPIA - The Southeast Asian Journal of Tropical Biology Jurnal Biodjati Biosaintifika: Journal of Biology & Biology Education The Journal of Society and Media BioEksakta : Jurnal Ilmiah Biologi Unsoed Jurnal Pengabdian Masyarakat Indonesia Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek) Molekul: Jurnal Ilmiah Kimia Biosaintifika: Journal of Biology & Biology Education Advance Sustainable Science, Engineering and Technology (ASSET) Indonesian Journal of Aerospace
Agus Nuryanto
Faculty of Biology, Universitas Jenderal Soedirman, Indonesia
Religion Aerospace Engineering Agriculture, Biological Sciences & Forestry Humanities Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Control & Systems Engineering Earth & Planetary Sciences Education Electrical & Electronics Engineering Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Public Health Social Sciences Veterinary

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Molecular Characterization Reveals Genetic Differences Between Wild and Captive Populations of Mandiangin Giant Gourami (Osphronemus goramy) Nuryanto, Agus; Susilo, Maria Bramasti; Amurwanto, Adi
Jurnal Biodjati Vol 4 No 1 (2019): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v4i1.3942

Abstract

  Captive population of Mandiangin giant gourami (Osphronemus goramy Lac.) is a new strain developed from wild pop-ulations in Riam Kanan Dam. No much is known about the genetic constituent of this strain compared to the parental population. This re-search was done to obtain information whether genetic alteration has occurred in captive population compared to their wild parental pop-ulation. Wild population was caught from Riam Kanan Dam, while captive population was collected from Balai Perikanan Budidaya Air Tawar (BPBAT) “Freshwater Aquaculture Centre” Mandiangin, South Kalimantan. The captive population of Batanghari strain from BPBAT Sungai Gelam, Jambi was also analyzed. Molecular assess-ment was done using CO1 PCR-RFLP which was developed by digest-ing the amplicon of the CO1 gene from all populations using two re-striction enzymes, namely HindIII and TaqI and resulted CO1-HindIII and CO1-TaqI RFLP markers. All tested populations showed uniform patterns of those CO1-RFLP markers but with different fragment sizes among populations. This means all resulted RFLP markers were mono-morphic in each populations but polymorphic among giant gourami populations. In other words, no genetic diversity within population but substantial genetic differences was observed between wild and cap-tive Mandiangin populations. This proved that genetic alteration has occurred in Mandiangin captive populations compared to the wild pa-rental giant gourami population. In conclusion, captive and wild pop-ulations of Mandiangin giant gourami were genetically different. This result is vital as scientific base for future development of inland fish-eries, especially for breeding strategy of Mandiangin giant gourami. 
Phylogenetic Relationships of Ornamental Chaetodontidae in The South Coastal of West Java, Indonesia Ihya, Silma Safira; Nuryanto, Agus; Prabowo, Romanus Edy; Bhagawati, Dian; Kusbiyanto, Kusbiyanto
Jurnal Biodjati Vol 5 No 1 (2020): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v5i1.5759

Abstract

Marine ornamental trade has been developing for several years with Indonesia as one of the major exporter countries. Among the popular marine ornamental fish in the local and international markets belong to the family of Chaetodontidae. The existence of this family has been reported from several areas in Indonesia. However, no information is available in terms of their phylogenetic relationships of the fish from the south coast of West Java. The aim of this research was to asses the phylogenetic relationships among ornamental fish members of Chaetodontidae in the South Coast of West Java. Fish samples were collected from two trading sites Ujung Genteng and Taman Manalusu. Collected fish were identified using a previously published study. The evolutionary relationships among species were analyzed statistically through phylogenetic analysis based on maximum parsimony algorithm and Kimura 2- Parameter substitution model as implemented in PAUP 4.0 software. Tree branching pattern supported by 1000 bootstraps pseudo-replicates and out-group comparison. Daschyllus trimaculatus used as outgroup species. Based on the study, Chaetodontidae from the South Coast of West Java formed one monophyletic group compared to Dascylus trimaculatus with Chelmon rostratus was the basal species. All the remaining species were the derived species. There are CI and RI value gained. The consistency index (CI) value of this family is 0.5833, and the retention index (RI) is 0.5082. 
Co-Authors Abdalla Mohammed, Mohammed Abdul Rahman Abulias, M Nadjmi Adi Amurwanto AGUS HERY SUSANTO Ali Suman Andareswari, Novi Atang, Atang BUDI SETIADI DARYONO D, Blohm D. Soedharma, D. Diah Mustikasari Diana Retna Utarini Suci Rahayu, Diana Retna Utarini Suci Dwi Nugroho Wibowo Elly Tuti Winarni Endang Hilmi Farida Nur Rachmawati Fathurahman, Fabian Rizky Ghifari, Muhammad Iqbal Handayani, D.P.H. Hendro Pramono Ihya, Silma Safira Ikegwu, Chukwudi Indarmawan Indarmawan, Indarmawan Kusbiyanto Kusbiyanto, Kusbiyanto Mahmoud, Hanan Hassan Alsheikh Moh. Husein Sastranegara Nabila, Afra Nael Huda Qonita Norman Arie Prayogo, Norman Arie Nuryadi, Dedi Petrus Hary Tjahja Soedibja Pulungsari, A.E. Pulungsari, Anastasia Endang Pulungsari, Anatasia Endang Purnama Sukardi Purry, Dyah R Siti Rukayah Rofiqoh, Aswi Andriasari Romanus Edy Prabowo Rosita, Rita Eka Samosir, Ganda Sastranegara, M.H. Sri Lestari Suryaningsih, Suhestri Susilo, Maria Bramasti SUTRISNO Syakuri, Hamdan Tuti Winarni, Elly Windiarini Lestari