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All Journal Bioma : Berkala Ilmiah Biologi Jurnal Agro JURNAL AGROHITA Abditani : Jurnal Pengabdian Masyarakat Jurnal Agrotek Indonesia Jurnal Agroplasma ZIRAA'AH MAJALAH ILMIAH PERTANIAN Jurnal Ilmiah Wahana Pendidikan Jurnal Budiman: Pembangunan dan Pengabdian Masyarakat Nusantara
Ani Lestari
Universitas Singaperbangsa Karawang
Religion Agriculture, Biological Sciences & Forestry Humanities Control & Systems Engineering Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Industrial & Manufacturing Engineering Social Sciences Other

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Journal : Jurnal Agroplasma

 1 
Pengaruh Beberapa Formulasi Sitokinin Terhadap Penyediaan Bibit dan Pertumbuhan Eksplan Tanaman Pule Pandak (Rauvolfia serpentina (L.) Benth. ex Kurz) Secara In Vitro Anita Widya Karyaningtyas; Ani Lestari; Edhi Sandra
JURNAL AGROPLASMA Vol 10, No 1 (2023): JURNAL AGROPLASMA VOLUME 10 NO 1 TAHUN 2023
Publisher : UNIVERSITAS LABUHANBATU

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/agroplasma.v10i1.3914

Abstract

Propagation of pule pandak (Rauvolfia serpentina (L.) Benth. Ex Kurz) conventionally shows growth from seeds and stem cuttings of less than 15%. The low growth percentage is due to the seeds have hard coats, so the germination of the seeds is very low. One of the technologies that can be used and provides hope in the supply of seeds in large quantities and in a relatively short time is the in vitro culture technique. The aim of this study was to determine does the several cytokinin formulations administration affected the growth of pule pandak (Rauvolfia serpentina (L.) Benth. ex Kurz) plant explants. The study was conducted at Esha Flora, Bogor from April 2022 to July 2022. The experiment used a single factor Completely Randomized Design (CRD) consisting of 1 factor using 4 combinations of cytokinins (2-IP, TDZ, BAP and Kinetin) and repeated 10 times so that there are 40 experimental units for 12 weeks. The data obtained were then analyzed using the ANOVA test at a significant level of 5%. The results showed that the S1 treatment (Modified MS Media + 2-IP 2 mg/l + TDZ 0.1 mg/l + BAP 0.5 mg/l and Kinetin 0.5 mg/l) was the best cytokinin formulation to produce the highest average number of shoots was 8.87 shoots, the highest explant height was 2.49 cm and the fastest average of somatic embryogenesis emergence time was 23.3 DAP. Somatic embryo phase in treatment S1, S2 and S3 experienced globular, heart and torpedo phases. Keywords: Cytokinine, Somatic Embryogenesis, Tissue Culture, Rauvolfia serpentina (L.) Benth. Ex Kurz
Respon Pertumbuhan Miselia F2 pada Media PDA dan F3 pada Baglog Beberapa Nomor Isolat Harapan Jamur Merang (Volvariella volvaceae) FAPERTA UNSIKA secara In Vitro Widya Pramudita; Ani Lestari; Devie Rienzani Supriadi
JURNAL AGROPLASMA Vol 10, No 1 (2023): JURNAL AGROPLASMA VOLUME 10 NO 1 TAHUN 2023
Publisher : UNIVERSITAS LABUHANBATU

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/agroplasma.v10i1.4214

Abstract

Straw mushroom (Volvariella volvaceae) is a type of edible mushroom that is great demand because it has good nutrition and high economic value. In the cultivation of straw mushroom, pure culture is needed which is free from contamination and has superior genetic characteristics both in terms of quality and quantity, which will then produce superior straw mushroom seeds. Superior seeds are factors that affect the production and yield of straw mushroom. The purpose of this study was to obtain one hopeful isolate of the straw mushroom Faperta UNSIKA that gave the best mycelial growth in vitro. The research was conducted at the Laboratory of Biotechnology and Plant Breeding, Faculty of Agriculture, Singaperbangsa Karawang University, located in Puseurjaya Village, Telukjambe Timur District, Karawang Regency and start from December 2022 - February 2023. The research method used was experimental with a single factor Randomized Block Design (RBD). There are 7 treatments, namely A (White Elder), B (Semi Elder), C (FP016), D (FP018), E (FP019), F (FP020) and G (FP021). Each treatments was repeated 5 times. The effect of the treatment was analyzed by analysis of variance and if the F test at 5% level was significant, to find out the best treatment, proceed with the DMRT (Duncan Multiple Range Test) at the 5% level. The results showed that there was a significant effect of some numbers hopeful isolate straw mushroom Faperta UNSIKA on the growth of F2 mycelia on PDA media and F3 on baglog in vitro. Treatment A (White Elder) gave the highest yield on the growth diameter of F2 mycelia on PDA media of 6,40 cm with the highest growth rate of 1,91 cm/day and treatment E (FP019) gave the highest yield on the growth length of F3 mycelia baglog of 13,21 cm with the highest growth rate of 2,70 cm/day. Keywords: Isolate Faperta UNSIKA, Mycelia, Straw Mushroom
Co-Authors Abdulloh Yasin Adi Setiadi Amrina Rosada Anggi Ayu Anggraeni Anggini Riyaldi Putri Anggrita Eka Puspitasari Anita Widya Karyaningtyas Annisa Nanda Ariati Anwar Puadi Aullia Oktaviani Bastaman Syah Cindy Maria Margaretheresia Simanjuntak Darso Sugiono Dedi Ruswandi Devie Rienzani Supriadi Devie Rienzani Supriadi Edhi Sandra Elia Azizah Endang Hadipoentyanti Fawzy Muhammad Bayfurqon Gita Erina Apriyanti Widiyanto Hayatul Rahmi Hilda Wijaya Iqbal Fajarudin Isna Lutfiani Joseph Safri Samsudin Munthe Lilik Listiani Malik Tsaqafi Mawaddah, Syerin Kusuma Moh Atoilah Muhammad Syafi’i Muharam Neneng Farida Solihat Novia Hermawati Nurcahyo Widyodaru Nurcahyo Widyodaru Nurlia Hasna Nabilah Prameswari Hafidz Hidayat Rakim Adiansyah Rimba Ghazali Bungsu Sinatria Rizfi Yusuf Assyafa Rommy Andhika Laksono Rommy Andhika Laksono Santikasari Santikasari Shafira Khansa Lutfiah Solahudin Sri Suhesti Sri Suhesti Sugiarto Sugiarto Sulandjari, Kuswarini Sulistyo Sidik Purnomo Syakilah Aini Zuhroh Tatang Surjana Tia Setiawati Tiara Kasih Apriliani Zarkati Tita Puspitasari Vera Octavia Subardja Vera Oktavia Subardja Vera Oktavia Subarja Vivian Rizky Hassan Widya Pramudita Yayu Sri Rahayu