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All Journal Jurnal Riset Kesehatan Journal of Health (JoH) Syifa' MEDIKA: Jurnal Kedokteran dan Kesehatan Media Ilmu Kesehatan JI-KES (Jurnal Ilmu Kesehatan) Indonesian Journal of Biology Education Jurnal Kesehatan Saelmakers PERDANA (JKSP) Medika Kartika : Jurnal Kedokteran dan Kesehatan Jurnal Kefarmasian AKFARINDO Pharma Xplore : Jurnal Sains dan Ilmu Farmasi Jurnal Abdi Insani Prosiding Conference on Research and Community Services Jurnal Ilmu Kesehatan MAKIA Jurnal Kesehatan Tambusai Jurnal Pengabdian kepada Masyarakat International Journal of Advanced Health Science and Technology Jurnal Kesehatan Rajawali Jurnal Ilmu Kesehatan MAKIA
Sepvianti, Wiwit
STIKES GUNA BANGSA YOGYAKARTA
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Pemeriksaan Antibody Dengue Pada Darah Donor di PMI Kabupaten Sleman Dengan Metode Rapid Test Serafica Btari Chrisityani Kusumaningrum; Wiwit Sepvianti
Jurnal Kesehatan Saelmakers PERDANA (JKSP) Vol. 5 No. 1 (2022): Jurnal Kesehatan Saelmakers PERDANA
Publisher : Fakultas Ilmu Kesehatan Universitas Katolik Musi Charitas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32524/jksp.v5i1.390

Abstract

Dengue Hemorrhagic Fever (DHF) is a disease caused by Dengue Virus (DENV), which concluding serotypes (1-4) through mosquito bites and transmitted into humans. DHF is endemic and has increasing cases every year in almost every region in Indonesia, such as Kabupaten Sleman. Currently, screening for Infectious Diseases Transmitted by Blood Transfusion in blood donor only requires Hepatitis B, Hepatitis C, HIV, and Syphilis, despite any other diseases such as Dengue. Therefore, this study needs to detect the IgG/IgM Dengue antibodies on blood donor obtained from PMI Sleman to ensure safety and good quality of blood donor. This research method is used descriptive observational research and the samples are obtained from PMI Sleman. Examining for Dengue IgG/IgM antibodies for donor blood samples was accomplished by the rapid test method. The results showed that all blood donor samples were non-reactive from Hepatitis B, Hepatitis C, HIV, and Syphilis as Infectious Diseases Transmitted by Blood Transfusion. There were 16 (51,72%) blood samples that were reactive of IgG/IgM Dengue and 13 (44,83%) blood samples were non-reactive IgG/IgM Dengue. The reactive blood samples divide into four blood groups, there were 7 (24,14%) samples of blood type A,Rh+; 1 (3,45%) sample of blood type B, Rh+; 2 (6,90%) samples with blood type B,Rh+; 2 (6,90%) samples with blood type AB,Rh+ and 6 (20,69%) samples with blood type of AB, Rh+. Moreover, the reactive donor blood samples divide as 15 (24,14%) samples were in the 17-30 years-old age group and there was 1 (3,45%) was from the 31-40 years-old age group.
UJI AKTIVITAS ANTIBAKTERI SENYAWA 4 DIMETILAMINOKALKON TERHADAP BAKTERI STAPHYLOCOCCUS AUREUS DAN ESCHERICHIA COLI ATCC WIWIT SEPVIANTI
Jurnal Kesehatan Tambusai Vol. 2 No. 4 (2021): Desember 2021
Publisher : Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/jkt.v2i4.3076

Abstract

Introduction: Pathogenic bacteria infection are the main cause of human disease, for example diphtheria, pneumonia, cholera, tuberculosis, dysentery and typhus. Giving antibiotics is the treatment for infectious diseases caused by bacteria, but the treatment of these infections became more difficult because of the drug resistant strains. This is what drives the development of new antibiotic compounds. Chalcone is one of the potential candidates as an antibacterial. Objective: Testing Antibacterial acitivy of 4-dimethylchalcone against Staphylococcus aureus and Escherichia coli ATCC bacteria. Methods: In this study, an antibacterial activity of 4-dimethylaminocahlcone was test at The methods that used to evaluate antibacteria activity of chalcone is agar diffusion. The variation of the chalcone concentration used was 0.625; 1.25; 2.5 and 5%. Results: The 4-dimethylaminocalcone compound showed moderate antibacterial activity against gram-negative bacteria (Escherichia coli ATCC) with the highest inhibitory zone diameter of 8 mm and and percentage of inhibition ability of 61.54%. at a concentration of 5% while in gram-positive bacteria (Staphylococcus aureus ATCC) was weak with a inhibitory zone diameter of only 0.7 mm and percentage of inhibition ability was only 3.89%. Conclusion: Antibacterial tests of 4-dimethylaminocahlcone showed that chalcone increased the antibacterial activity at Escherichia coli ATCC bacteria but decreased Staphylococcus aureus ATCC bacteria. Keyword: antibacterial, chalcone, Escherichia coli , Staphylococcus aureus
GAMBARAN KADAR HEMOGLOBIN PADA SEDIAAN DARAH LENGKAP DI PMI KABUPATEN SLEMAN PROVINSI D.I YOGYAKARTA Ana Nur ‘Aini; Wiwit Sepvianti; Serafica Btari Christiyani Kusumaningrum
Prosiding Conference on Research and Community Services Vol 1, No 1 (2019): Prosiding Conference on Research and Community Services)
Publisher : STKIP PGRI Jombang

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Abstract

Darah lengkap merupakan sediaan darah yang mengadung komponen utama berupa eritrosit, trombosit dan faktor pembekuan (V dan VIII). Sediaan darah ini umumnya diberikan pada pasien yang mengalami perdarahan masif, dikarenakan darah lengkap dapat menggantikan kehilangan darah yang terjadi dan memaksimalkan oksigenasi pada organ tubuh pasien. Darah lengkap dapat disimpan hingga 30 hari, pada suhu 2- 6˚C di dalam blood bank. Akan tetapi, masa penyimpanan darah berdampak langsung pada perubahan kualitas darah. Hal ini disebabkan terjadinya stress cells yang memicu lisis pada sel darah. Adapun lisis yang terjadi pada eritrosit akan mempengaruhi kadar hemoglobin dalam darah sedangkan kadar hemoglobin merupakan salah satu indikator kualitas darah. Apabila kadar hemoglobin kurang dari 12,5g/dL darah tidak dapat diberikan pada pasien. Pada penelitian ini dilakukan pengamatan terhadap kadar hemoglobin pada sediaan darah lengkap yang menggunakan antikoagulan CPDA-1. Adapun pengukuran kadar hemoglobin dilakukan sejak masa penyimpanan 0-30 hari menggunakan instrumen hematology analyzer sysmex xs 800i. Hasil pengukuran kadar hemoglobin selama 30 hari penyimpanan  menunjukkan bahwa kadar hemoglobin relatif stabil. Hal ini karena penurunan yang terjadi tidak terlalu signifikan, yaitu  berkisar antara 0,6-2,0%. Oleh karena itu, dapat dinyatakan bahwa  selama 30 hari penyimpanan kadar hemoglobin masih baik digunakan untuk berbagai kondisi medis yang memerlukan transfusi darah lengkap
GAMBARAN JUMLAH TROMBOSIT PADA SEDIAAN DARAH THROMBOCYTE CONCENTRATE (TC) SELAMA MASA SIMPAN 5 HARI Rizkia Anggini; Wiwit Sepvianti; Meyta Wulandari
Prosiding Conference on Research and Community Services Vol 1, No 1 (2019): Prosiding Conference on Research and Community Services)
Publisher : STKIP PGRI Jombang

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Abstract

Thrombocyte Concentrate (TC) merupakan salah satu produk darah yang berisi trombosit pekat hasil dari pemisahan darah lengkap atau trombofheresis. Produk TC direkomendasikan untuk meningkatkan trombosit pada indikasi yang ditunjukkan ke pasien terutama pada pasien trombositopenia hingga Demam Berdarah Dengue (DBD). Berbeda dengan produk darah lainnya trombosit hanya dapat disimpan selama 5 hari pada suhu 20-24ºC. Selain itu trombosit juga sensitif terhadap agregasi yang mengakibatkan hilangnya viabilitas sel, sehingga harus disimpan pada agitator. Agitasi trombosit juga mengurangi produksi laktat, dan meminimalisir penurunan pH. Tingginya produksi asam laktat dapat mengakibatkan perubahan kualitas produk TC. Terdapat 2 indikator penelitian pada ini yaitu untuk mengetahui gambaran jumlah trombosit dan kadar pH selama masa simpan 5 hari. Instrumen penelitian dilakukan dengan mengamati langsung dan mencatat hasil pemeriksaan menggunakan alat Hematology analyzer sysmex 800i dan pH meter. Hasil dari pemeriksaan jumlah trombosit mengalami peningkatan yang signifikan pada pemeriksaan hari ke-3 dan hari ke-5 sebanyak 6% dari pemeriksaan hari pertama. Sedangkan pada pengukuran pH terjadi penurunan 4% namun masih dalam batas normal sesuai dengan peraturan menteri kesehatan No. 91 tahun 2015 tentang Standar Pelayanan Transfusi Darah dengan kadar pH normal > 6,4. Faktor yang mempengaruhi diantaranya tingginya produksi asam laktat karena metabolisme glukosa yang banyak digunakan oleh TC selama penyimpanan sehingga persediaan glukosa untuk trombosit berkurang. Dari pemeriksaan satu kantong produk TC disimpulkan bahwa jumlah trombosit mengalami peningkatan dan terjadi penurunan pH selama masa simpan 5 hari.
SINTESIS SENYAWA 4’-HIDROKSI-4-METOKSIKALKON DAN UJI AKTIVITAS ANTIBAKTERI TERHADAP BAKTERI KONTAMINAN PRODUK DARAH Wiwit Sepvianti; Serafica Btari Christiyani Kusumaningrum
Pharma Xplore : Jurnal Sains dan Ilmu Farmasi Vol 7 No 1 (2022): Pharma Xplore : Jurnal Sains dan Ilmu Farmasi
Publisher : Fakultas Farmasi Universitas Buana Perjuangan Karawang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36805/jpx.v7i1.2331

Abstract

Despite of the blood products are crucial for health, it cannot be separated from the risks of bacterial contamination. The bacterial contamination obtained from collecting and processing blood processing. Various species of positive and negative Gram bacteria are known to be bacterial contaminants in blood products such as thrombocyte concentrate, packed red cell, dan whole blood. Therefore, it is essential to find an antibacterial compound that could inhibited the growth of bacterial contaminant in blood products. Chalcone compounds are known to become one of antibacterial since they have an unsaturated-ketone-group which acts as an antibacterial because it can damage the bacterial cell wall. The presence of hydroxyl group has important role for inhibiting bacterial contaminant growth as well. In this research, 4’-hydroxy-4-methoxychalcone was synthesized through the Claisen-Schmidt condensation reaction. The characterization of the chalcone compounds was carried out by mass spectroscopy using gas chromatography-mass spectroscopy (GC-MS) and identification of functional groups using Fourier transform infrared (FTIR). Bacterial contaminants from blood products that were used: Acinetobacter, Escherichia coli, Bacillus sp, and Staphylococcus aureus. In addition, Staphylococcus aureus from the American Type Culture Collection (ATCC) was used as comparing isolates. The antibacterial activity was analyzed by inhibition area method. The results showed that 4’-hydroxy-4-methoxychalcone successfully synthesized with the reaction product in yellow crystal formation with yield of 72,80%, relative purity 100%, and m/z 254. Antibacterial activity of chalcone compounds at various concentrations of 6.25 ppm; 12.5 ppm and 25 ppm against Gram-positive and Gram-negative bacteria are still relatively weak because the percentage of inhibition ability achieved is below 50%.
Evaluasi Kualitas Sediaan Packed Red Cells Hasil Pemrosesan Metode Top and Top Wiwit Sepvianti; Serafica Btari Christiyani Kusumaningrum; Ikrimah Nafilata; Arum Sari; Aulia Rahman
JI-KES (Jurnal Ilmu Kesehatan) Vol. 6 No. 1 (2022): JI-KES (Jurnal Ilmu Kesehatan)
Publisher : STIKes Hafshawaty Pesantren Zainul Hasan Probolinggo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33006/ji-kes.v6i1.333

Abstract

ABSTRAKSediaan packed red cells dapat diperoleh melalui beberapa metode pemrosesan di antaranya adalah metode top and top, top and bottom serta leukodepleted. Di antara ketiga metode tersebut metode top and top adalah metode yang paling banyak digunakan di berbagai unit pelayanan darah. Pada penelitian ini dilakukan evaluasi kualitas packed red cells metode top and top dengan parameter uji kadar hemoglobin, residual leukosit, hematokrit dan pH darah. Adapun metode penelitian yang digunakan adalah observasional. Hasil pengujian menunjukkan bahwa rerata kadar hemoglobin diperoleh sebesar 49,02±5,51gram/unit, telah melebihi standar minimal hemoglobin yaitu 45gram/ unit. Selaras dengan kadar hemoglobin, kadar hematokrit juga berada pada kisaran ideal 65-75% dengan rerata kadar hematokrit sebesar 70,45±5,36%. Nilai pH darah kantong terukur berada pada rentangan 7,348±0,064, nilai ini sesuai dengan kriteria packed red cells yang baik yaitu memiliki kadar pH>6,71. Adapun parameter yang belum sesuai dengan standar adalah kadar residual leukosit.  Packed red cells yang baik seharusnya memiliki residual leukosit <1,2x109sel/unit sedangkan pada penelitian ini diperoleh rerata residual leukosit sebesar (3,66±0,41)x109sel/unit yang menunjukkan jumlah leukosit tertinggal masih berada pada jumlah yang cukup banyak. Berdasarkan evaluasi yang dilakukan dapat dinyatakan bahwa packed red cells hasil pemrosesan top and top memiliki kualitas baik dari segi kadar hemoglobin, hematokrit dan pH darah namun kurang baik ditinjau dari level residual leukositnya.Kata kunci: hemoglobin, hematokrit, ph darah, packed red cells, residual leukositABSTRACTPacked red cells(PRC) could be obtained by several processing methods such as top and top, top and bottom and leuko-depleted methods. However, the most widely used method in the blood service unit is the top and top method. Therefore, this research was conducted to evaluate the quality of PRC produced from the top and top method with the haemoglobin level, leukocytes residual, hematocrit, and blood pH parameters. The research method used is observational. In addition, this study was performed to know the QC standard accomplishment. The results showed that the average haemoglobin level was 49,02±5,51gram/unit and reached the minimum (45gram/unit).  The average of hematocrit also reached the minimum standard (65-75%) since it obtained  70,45±5,36%. The pH also had included in the sufficient criteria since it had 7,348±0,064, as pH needs to be >6,71. However, the level of the residual leukocyte had not reached the minimum standard yet (1,2x109cells/unit) since the residual leukocytes of PRC in this research were obtained (3,66±0,41)x109cells/unit. It showed that there were a high proportion of leukocytes left. Overall, based on the evaluation, it was concluded that the PRC produced from the top and top method had better quality in the haemoglobin level, hematocrit, and pH but not in the residual leukocyte level. Keywords: hemoglobin, hematocrit, blood pH, packed red cells, residual leukocytes  
Anti-Bacterial Activity of Novel Synthesized Chalcone Against S. epidermidis and S. aureus Isolated from Packed Red Cell Blood Product Aulia Rahman; Wiwit Sepvianti; Serafica Btari Christiyani Kusumaningrum; Arum Sari; Mohd Nazil Salleh
International Journal of Advanced Health Science and Technology Vol. 2 No. 6 (2022): December
Publisher : Forum Ilmiah Teknologi dan Ilmu Kesehatan (FORITIKES)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35882/ijahst.v2i6.174

Abstract

Bacterial contamination in blood product was still a problem in Indonesia. Various studies to improve the safety of blood product by inhibiting the bacterial growth in blood product was developed. Chalcone, is one of the natural compounds that has ability to inhibit the bacterial growth, so it can be possible to use that compound for anti-bacterial use in the future. This study aim is to evaluate the anti-bacterial activity of novel synthesis chalcone (E)-1-(4-hydroxyphenyl)-3-phenylprop-2-en-1-one against S. epidermidis and S. aureus isolated from packed red cell blood product. A novel chalcone was synthesized by Claisen-Schmidt Methods while anti-bacterial activity test was done by Agar Diffusion Methods. Chalcone that has been synthesized then characterized by Fourier Transform Infra-Red (FTIR) Methods and Gas Chromatography – Mass Spectrometry (GC-MS). Chalcone was synthesized through a condensation reaction between an aldehyde (benzaldehyde) and ketone (acetophenone) with NaOH as alkaline catalyst. The results from sedimentary analysis using FTIR showed an absorption of the C=C group in the typical medium of chalcone compounds, at the wave number of 1516.96 cm-1. The results of GC-MS characterization resulted a GC chromatogram with one peak with time of retention (tR) 19.68 minutes and relative purity 100%. MS mass spectrum shows that the molecular ion (M+) of this compound was detected at 224 which equivalents to the molecular weight of the chalcone compound. Anti-bacterial test showed that the synthetic chalcone compound with 5% concentration had an inhibitory ability of 56.02% on S. epidermidis and 54.10% on S. aureus, while 2.5% chalcone concentration had an inhibitory ability of 29.17% on S. epidermidis and 50.45% on S. aureus, and 1.25% chalcone concentration had inhibitory ability 0% on S. epidermidis and 37.27% on S. aureus.
PENGEMBANGAN DESA SIAGA DONOR DARAH MELALUI PROGRAM PEMERIKSAAN DAN PENDATAAN GOLONGAN DARAH WARGA DUSUN MANGGONG, KEPUHARJO, CANGKRINGAN, SLEMAN Gravinda Widyaswara; Wiwit Sepvianti; Aulia Rahman; Kumara Rahmawati Zain; Arif Tirtana; Ikrimah Nafilata
J-ABDI: Jurnal Pengabdian kepada Masyarakat Vol. 2 No. 9: February 2023
Publisher : Bajang Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53625/jabdi.v2i9.4886

Abstract

The Allert Village is a village that has a Village Health Post or health professionals who are ready to carry out community empowerment, encourage health-oriented development, and be the first referral center for quality health services for the community and health emergencies in the Village. In moving towards the allert village, it is necessary to study various existing community-based activities such as posyandu, polindes, village medicine post, health fund, and inter-guard preparedness, as a starting point for development towards an allert village. One of the Allert Village programs currently being developed is the Blood Donor Allert Viilage. In this program, village communities are prepared to be responsive in dealing with health problems that require blood donors. The development of the Blood Donor Allert Village was initiated with outreach activities, data collection and examination of the blood group of residents in each hamlet. Data on residents' blood groups is then submitted to village midwives, sub-districts and health centers as a data base for the Blood Donor Allert Village. Blood group data collection for the residents of Manggong, one of the hamlets in Kepuharjo Village, obtained the following results: out of a total of 256 r esidents, 62.50% had their blood type recorded while 37.50% was not. As for the 62.50% of Dusun Manggong residents whose blood type was recorded, 26.87% had blood type A; 28.13% blood type B; 41.25% have blood type O and only 3.75% have blood type AB
Pengaruh Frekuensi Mengkonsumsi Kopi Dengan Kadar Hemoglobin Calon Pendonor Laki-Laki Di PMI Kabupaten Sleman Relita Pebrina; Wiwit Sepvianti; Serafica Btari Christiyani Kusumaningrum; RIfail
Jurnal Ilmu Kesehatan MAKIA Vol 11 No 2 (2021): Jurnal Ilmu Kesehatan MAKIA
Publisher : LPPM ISTeK ICsada Bojonegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (329.244 KB) | DOI: 10.37413/jmakia.v11i2.173

Abstract

ABSTRAK Donor darah adalah proses pengambilan darah dari seseorang secara sukarela untuk disimpan di bank darah yang kemudian digunakan untuk keperluan transfusi darah. Sebelum pelaksanaan pengambilan darah (aftap), calon donor terlebih dahulu diseleksi untuk mengetahui apakah memenuhi syarat/ kriteria yang ditentukan. Seleksi donor diantaranya adalah pemeriksaan kadar hemoglobin (Hb). Kadar Hb setiap orang berbeda-beda, hal ini dapat dipengaruhi oleh beberapa faktor salah satunya minuman yang populer saat ini adalah kopi. Tujuan penelitian ini adalah mengetahui pengaruh frekuensi minum kopi dengan kadar hemoglobin calon pendonor laki-laki. Jenis penelitian ini bersifat deskriptif korelational dengan pendekatan cross sectional. Sampel penelitian berjumlah 50 responden ditentukan dengan concecutive sampling. Hasil yang didapat dalam penelitian ini yaitu frekunesi mengkonsumsi kopi responden 4 kali dalam seminggu memiliki kadar Hb normal 40,0% lebih banyak dari kadar Hb rendah 2,0% dan kadar Hb tinggi 0,0%, sedangkan responden dengan perilaku >4 kali minum kopi dalam seminggu memiliki kadar Hb normal 30,0% lebih banyak dari kadar Hb rendah 4,0% dan kadar Hb tinggi 0,0%. Responden dengan perilaku 6 kali minum kopi dalam seminggu memiliki kadar Hb normal 20,0% lebih banyak dari kadar Hb rendah 4,0% dan kadar Hb tinggi 0,0%. Kesimpulan penelitian ini adalah terdapat pengaruh frekuensi konsumsi kopi dengan kadar hemoglobin calon pendonor laki-laki dengan nilai signifikasi 0.000.
Antibacterial Activity of 4-hydroxychalcone Against Bacterial Contaminant of Packed Red Cells Serafica Btari Christiyani Kusumaningrum; Wiwit Sepvianti; Relita Pebrina; Aulia Rahman
Indonesian Journal of Biology Education Vol 5, No 2 (2022): INDONESIAN JOURNAL OF BIOLOGY EDUCATION
Publisher : Universitas Tidar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31002/ijobe.v5i2.7158

Abstract

The bacterial contamination of blood products is still become a serious problem in consideration, thus it can cause the risk of blood transfusion, namely bacterial sepsis. Packed red cells is blood product and potentially contain bacterial contaminant. The source of bacterial contamination can come from the skin disinfection process in less aseptic during blood collection, donor bacterimia, and  blood processing. In response, currently there have been many antibacterial compounds developments purposely to reduce the risk of bacterial contamination of blood products. This study aimed to observe the activity of 4-hydroxychalconecompounds in contaminant bacteria isolated from packed red cells blood products. Bacterial isolates isolated from the packed red cells were Staphylococcus aureusbased on cell morphology, biochemistry and colony shape. The antibacterial activity of 4-hydroxychalchone compound against Staphylococcus aureus isolates used the diffusion method. The results showed, there were antibacterial activity of 4-hydroxychalchone at a concentration of 2.5% 1.25% and 0.625% against packed red cells contaminant bacteria based upon the clear zone formed. In conclusion, the bacteria isolate was obtained from the PRC was gram positive bacteria, Staphylococcus aureus. The 4-hydroxychalcone had successfully synthesized and has antibacterial activity against S. aureus isolated from PRC, with the biggest inhibition power was 71,36% at 5,0% of chalcone concentration.
Co-Authors Ana Nur ‘Aini Andriyani, Yuni Arif Tirtana Arif Tirtana Arif Tirtana Arum Sari Arum Sari Arum Sari Aulia Rahman Aulia Rahman Bria, Theodosia Claranty Nahak Btari C.K, Serafica Christiyani Kusumaningrum, Serafica Btari Diani Mentari Gravinda Widyaswara Gravinda Widyaswara Hae, Shinny Love Laga Ikrimah Nafilata Ikrimah Nafilata Kodo, Jemi Arivitriyanto Livingston Kumara Rahmawati Zain Kusumaningrum, Serafica Btari Christiyani Lusia Peni Lamen Meyta Wulandari Mohd Nazil Salleh Nafilata, Ikrimah Nurpratami, Diah Pebriana, Relita Pebriani, Relita Pebrina, Relita Relita Pebrina Relita Pebrina RIfail Rizkia Anggini Selasih Putri Isnawati Hadi Siti Arifah Sunartono Sunartono sunartono sunartono Taib Djafar Tirtana, Arif Widyaswara, Gravinda Zain, Kumaha Rahmawati