Article Per Year (5 Year)
p-Index From 2021 - 2026
0.817
P-Index
This Author published in this journals
All Journal Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Biota Jurnal Biodjati Biosaintifika: Journal of Biology & Biology Education BioEksakta : Jurnal Ilmiah Biologi Unsoed Molekul: Jurnal Ilmiah Kimia
Hendro Pramono
Faculty Of Biology, Universitas Jenderal Soedirman, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Veterinary

Published : 15 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 15 Documents
Search

 1   2 
Kualitas Yoghurt dengan Penambahan Bifidobacterium sp. Bb2E Putri, Yolandina Salsabila; Kusharyati, Dyah Fitri; Pramono, Hendro
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 1 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2020.2.1.1802

Abstract

Yoghurt merupakan produk pangan fungsional hasil fermentasi dari susu yang melibatkan peran bakteri asam laktat (BAL) Lactobacillus bulgaricus dan Streptococcus thermophillus. Menurut beberapa penelitian terdapat BAL yang memiliki ketahanan lebih baik dalam saluran pencernaan manusia, yaitu Bifidobacterium sp.. Selain itu, Bifidobacterium sp. memiliki manfaat diantaranya adalah meningkatkan fungsi pencernaan, menurunkan kolestrol, sebagai antioksidan dan anti inflamasi, mengurangi gejala alergi, serta dianggap sebagai salah satu bakteri probiotik yang penting dalam tubuh manusia. Tujuan dari penelitian adalah mengetahui pengaruh penambahan Bifidobacterium sp. Bb2E terhadap kualitas yoghurt dan mengetahui konsentrasi Bifidobacterium sp. Bb2E yang terbaik dari segi organoleptik. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan 4 perlakuan dan 3 kali ulangan, sehingga terdapat 12 unit percobaan. Perlakuan yang diterapkan adalah perbandingan konsentrasi starter yoghurt yang berbeda dari BAL L. bulgaricus, S. thermophillus dan substitusi Bifidobacterium sp. Bb2E dengan perbandingan 2:2:0 (Y0), 2:2:1 (Y1), 2:2:2 (Y2), dan 2:2:3 (Y3). Variabel dalam penelitian ini, variabel bebas, yaitu konsentrasi Bifidobacterium sp. Bb2E dan variabel terikat, yaitu kualitas yoghurt. Parameter utama adalah kadar asam laktat. Parameter pendukung, yaitu total BAL pada yoghurt, nilai pH, dan sifat organoleptik pada yoghurt. Data diolah dengan Analysis of Variance (ANOVA) dengan taraf kesalahan 5%. Penambahan Bifidobacterium sp. Bb2E tidak memberi pengaruh nyata terhadap kualitas yoghurt dari segi kadar asam laktat, pH, dan total BAL dan yoghurt dengan starter Lactobacillus bulgaricus : Streptococcus thermophillus : Bifidobacterium sp. Bb2E dengan perbandingan konsentrasi 2:2:3 merupakan yoghurt yang disukai konsumen dengan nilai rata-rata tertinggi pada uji organoleptik.
Regional Variations in rpoB Gene Mutations and Their Association with Rifampicin Resistance in Mycobacterium tuberculosis Widodo, Widodo; Wahyono, Daniel Joko; Oedjijono, Oedjijono; Pramono, Hendro
Molekul Vol 20 No 1 (2025)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jm.2025.20.1.13215

Abstract

ABSTRACT. The increasing incidence of tuberculosis (TB) remains a global challenge, driven by the emergence of Mycobacterium tuberculosis resistance to antituberculosis drugs. This study investigated the relationship between rpoB gene mutations, rifampicin resistance levels, and the geographic origin of multidrug-resistant M. tuberculosis (MDR-TB) isolates. A total of 30 MDR-TB isolates were collected from the Central Java Provincial Health and Calibration Testing Laboratory between January and December 2023. Rifampicin resistance levels ranged from 5 to 80 µg/mL and were assessed using the microculture method, while DNA sequencing identified rpoB mutations. The results showed that rifampicin exposure significantly influenced rpoB mutation frequencies, with Ser531Leu (50%), His526 (16.7%), Leu511 (6%), Leu533 (6%), and Gln513 (6%) being the most common. Geographic variations were observed, with isolates from the ex-residencies Pekalongan, Pati, and Banyumas Residencies harboring seven, six, and four distinct mutations, respectively. Mutations such as Leu511Arg+His526Ser and Asp516Val were detected at low rifampicin concentrations (5–10 µg/mL), whereas Ser531Leu mutations dominated at moderate levels (20–80 µg/mL). These findings confirmed that rpoB mutations were influenced by both the region of origin and rifampicin resistance levels, providing critical insights for improving TB diagnosis and optimizing MDR-TB treatment strategies. Keywords: Mycobacterium tuberculosis, rifampicin resistance, rpoB gene mutation
Kemampuan Bakteri Halotoleran dari Sedimen Mangrove Pantai Logending dalam Pelarutan Fosfat dan Penambatan Nitrogen Ramadani, Adib; Oedjijono, Oedjijono; Pramono, Hendro; Pratiwi, Meyta
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 7 No 1 (2025): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2025.7.1.14854

Abstract

Lingkungan mangrove dapat menjadi sumber yang baik untuk mendapatkan bakteri halotoleran yang berpotensi sebagai agensia pupuk hayati dengan kemampuan melarutkan fosfat dan menambat N2 bebas. Tujuan penelitian ini adalah menguji toleransi isolat bakteri asal sedimen mangrove terhadap salinitas, menguji kemampuan isolat bakteri halotoleran dalam pelarutan fosfat dan penambatan nitrogen bebas, dan karakterisasi fenetik isolat bakteri halotoleran terpilih. Hasil penelitian menunjukkan bahwa dari 43 isolat yang diteliti, sebanyak 29 isolat toleran terhadap NaCl 5-7%. Dari 29 isolat, sebanyak 11 isolat bakteri halotoleran (>5% NaCl) diketahui mampu melarutkan fosfat dan menambat nitrogen dengan tiga isolat terpilih (LG36, LG60, dan LG66). Ketiga isolat tersebut menunjukkan efisiensi pelarutan fosfat masing-masing 125, 123,72, dan 126,92; dan kemampuan penambatan nitrogennya berturut-turut 38,02 ppm; 28,4 ppm; dan 51,93 ppm. Identitas ketiga isolat terpilih menunjukkan bahwa isolat LG60 merupakan spesies anggota genus Rhizobium, sedangkan isolat LG36 dan LG66 belum dapat diidentifikasi pada level genus. Kata kunci: fiksasi nitrogen, halotoleran, mangrove, pelarutan fosfat
Lactic Acid Bacteria from Mangrove Sediment Produce Bacteriocins Active Against Gram-Positive and Negative Bacteria Kusharyati, Dyah Fitri; Ariaputri, Fira Julian; Pramono, Hendro; Rovik, Anwar
Jurnal Biodjati Vol 8 No 1 (2023): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v8i1.24856

Abstract

Mangrove is a unique ecosystem. Only a few studies have explored the presence of lactic acid bacteria and their roles in mangrove ecosystems. From mangrove sediments at Logending Beach in Jawa Tengah (Indonesia), the Lactic Acid Bacteria (LAB) isolates LG-50, LG-107, and LG-114 were discovered. They produce bacteriocins. This study aimed to determine the characteristics of LAB isolates, antimicrobial activity, and physicochemical properties of bacteriocins. LAB isolates were characterized by morphology, physiology, and biochemistry. The production of bacteriocin was performed by salting-out method, followed by testing its antimicrobial activity against pathogenic bacteria. Isolates LG-50, LG-107, and LG-114 are thought to be in the Lactobacillus group. The crude bacteriocin can inhibit the growth of Gram-positive and negative bacteria. The average inhibition zones against Escherichia coli and Staphylococcus aureus were 16.67 mm and 22.17 mm, respectively. The crude bacteriocin tested positive for ninhydrin. It confirmed the crude bacteriocin was a protein and sensitive to the proteolytic enzyme. SDS-PAGE analysis presented the molecular weight of crude bacteriocin was 38 kDa. This present study supports the potential use of bacteriocin in the pharmaceutical and food industries.
Petroleum Degradation by Bacteria Explored from Logending Mangrove Sediments Apriliani, Herlina; Pramono, Hendro; Oedjijono, Oedjijono; Widodo, Wimbuh Tri; Kristianto, Sonny; Putri, Rury Eryna; Unsunnidhal, Lalu
Biota Vol 12 No 1 (2026): Jurnal Biota 2026
Publisher : Faculty of Science and Technology Universitas Islam Negeri Raden Fatah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19109/biota.v12i1.29245

Abstract

Oil spills resulting from shipping activities, tanker-based oil transportation, and fuel oil usage can cause coastal pollution, particularly in sensitive ecosystems such as mangroves. More than 90% of petroleum consists of hydrocarbons with complex carbon chain structures, making them difficult to decompose. Biological remediation using microorganisms offers a promising alternative for pollution mitigation, as microbes can degrade petroleum components and oxidizing hydrocarbons. This study aimed to evaluate the petroleum-degrading ability of selected bacterial isolates obtained from mangrove sediments at Logending Beach. The research employed experimental and survey methods. The primary parameter measured was Total Petroleum Hydrocarbons (TPH), while supporting parameters included pH and bacterial population density. The study consisted of several stages, including bacterial isolation, screening, and evaluation of the petroleum degradation capacity of selected isolates. The results identified two potential bacterial isolates capable of degrading crude oil. Isolate LG62 exhibited a degradation efficiency of 71.40%, while isolate LG105 showed a degradation efficiency of 57.10%. Petroleum concentrations of 2% (v/v) and 5% (v/v) were degraded more effectively than higher concentrations. Overall, the two bacterial isolates (LG62 and LG105) from Logending mangrove sediments demonstrated significant potential as bioremediation agents for petroleum hydrocarbon contamination.
Co-Authors A.A. Ketut Agung Cahyawan W AGUS HERY SUSANTO Agus Irianto Agus Nuryanto Agus Nuryanto Andareswari, Novi Apriliani, Herlina Ariaputri, Fira Julian Daniel Joko Wahyono Dini Ryandini Dyah Fitri Kusharyati Fitriyani, Nur Lu'lu Ghifari, Muhammad Iqbal Kristianto, Sonny Kusbiyanto Kusbiyanto Kusbiyanto Kusbiyanto, Kusbiyanto Moh Husein Sastranegara Moh. Husein Sastranegara Muhammad Iqbal Ghifari Nael Huda Qonita Nael Huda Qonita Novi Andareswari Oedjijono Oedjijono, Oedjijono Petrus Hary Tjahja Soedibja Petrus Hary Tjahja Soedibja Pratiwi, Meyta Putri, Yolandina Salsabila Ramadani, Adib Rovik, Anwar Rury Eryna Putri Syamsiar, Syamsiar Unsunnidhal, Lalu Widodo Widodo Wimbuh Tri Widodo