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All Journal International Journal of Renewable Energy Development ANNALES BOGORIENSES Jurnal Penelitian Kelapa Sawit Teknologi Indonesia Makara Journal of Technology Annales Bogorienses
Bambang Prasetya
Research Center for Biotechnology, LIPI, Cibinong, Bogor 16911
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Electrical & Electronics Engineering Energy Engineering Immunology & microbiology Materials Science & Nanotechnology Mechanical Engineering Medicine & Pharmacology

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Screening for Natural Producers Capable of Producing 1,3-Propanediol from Glycerol Andriani, Dian; Kusharyoto, Wien; Prasetya, Bambang; Willke, Thomas; Vorlop, Klaus Dieter
Annales Bogorienses Vol. 14 No. 1 (2010): Annales Bogorienses
Publisher : BRIN

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Glycerol is a renewable resource found as the main by-product in the transesterification of triglycerides and fat saponification. Due to the increased production of plant oils, especially palm oil in developing countries, and their larger use by the oleochemical industry, glycerol surpluses are on the world market and this may result in a decrease in glycerol price. As a consequence, biotechnological processes have been developed to convert this substrate into value-added products, such as 1,3-propanediol (1,3-PD). The microbial production of 1,3-PD could be competitive to chemical routes assuming that it is based on cheap raw material and an optimised process. In the screening for 1,3 PD–producing bacteria, raw glycerol as by-product from rapeseed oil processing unit was used as a carbon source compared with commercial glycerol. By using increasing concentration of both glycerols from 50 to 150 g/l, two potential bacteria were obtained from soil samples. BMP-1 was obtained from an enrichment culture using 50 g/l commercial glycerol, while BMR-1 was obtained from an enrichment culture using 100 g/l raw glycerol. The highest conversion yield obtained using the isolate BMP-1 was around 0.62 g 1,3-PD formed per mol glycerol consumed, and 0.73 mol 1,3-PD formed per mol glycerol using the isolate BMR-1. No bacteria were obtained from cultures using 150 g/l commercial and raw glycerol, respectively, which indicated that higher concentration of glycerol has inhibition effect. 
Optimization of Culture Conditions for Production of β-Mannanase by Strain Nonomuraea sp. ID06-379 using Submerged Substrate Fermentation Ratnakomala, Shanti; Yopi, Yopi; Suhartono, Maggy Thenawidjaja; Meryandini, Anja; Prasetya, Bambang
Annales Bogorienses Vol. 18 No. 2 (2014): Annales Bogorienses
Publisher : BRIN

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The objective of this study was to investigate the effect of media compositions on the production of β-mannanase by Nonomuraea sp. ID06-379. The study was focused on the influence of carbon, nitrogen, phosphorus and detergents on β-mannanase synthesis through manipulating media compositions on production medium. The results indicated that for carbon sources, locus bean gum (0.745 ± 0.036 U/ml) showed maximum mannanase activity. Malt extract was the best nitrogen source for producing β-mannanase (1.075 ± 0.006 U/ml), (NH4)2HPO4 as phosphate source (1.733 ± 0.026 U/ml) and Tween 80 (1.145 ± 0.003 U/ml) as surfactants effect on increasing permeability of bacterial cell membrane, enhancing membrane transport and excretion of extracellular enzymes into the production media. The results showed that 1% malt extract, 0.5% locus bean gum and 0.05% (NH4)2HPO4 were good substances for nitrogen source, carbon source and phosphate respectively. The highest production of β-mannanase by Nonomuraea sp. ID06-379 (5.33 U/mg) was reached in the medium optimization (Vogel’s minimal medium) contained the following ingredients: 0.5% locus bean gum, 1% malt extract and 0.05% (NH4)2HPO4, under submerged fermentation with shaking at 120 rpm and 28C for 2 days incubation.
Co-Authors Aliyah, Novin Anas Miftah Fauzi Anja Meryandini Anondho Wijanarko Budi Mulyono, Ary Changshin Sunwoo, Changshin Dian Andriani, Dian Djumali Mangunwidjaja Don-Hee Park, Don-Hee Dulbert Tampubolon, Biatna Dwi Susmiarni, Rika Edita Martini, Edita Ellia Kristiningrum Euis HERMIATI Febrian Isharyadi Heri Hermansyah I Nyoman Jaya Wistara Kang, Kyeong Eop Khaswar Syamsu Kusharyoto, Wien Kusharyoto, Wien Kyeong Kang, Kyeong Lanjar Sumarno M Baiquni M Samsuri, M M. Baiquni Maggy T Suhartono Maggy Thenawidjaja Suhartono Mardias, R. Misri Gozan Mohammad Nasikin Nasikin, M. Nastiti Siswi Indrasti Ono Suparno R Mardias, R Restu Wahono, Daryono Samsuri, M. SHANTI RATNAKOMALA Sriramulu GobiKrishnan, Sriramulu Sunarti, Ttiti C Surn-Teh Bark, Surn-Teh Titi C. Sunarti Tjahyo Eka Darmayanti, Nur Utari Ayuningtyas Vorlop, Klaus Dieter Vorlop, Klaus Dieter Wasrin Syafii Widya FATRIASARI Wilke, Thomas Willke, Thomas Wulansari, Nuri YOPI YOPI