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All Journal AGRISAINS Biotika: Jurnal Ilmiah Biologi AL KAUNIYAH Proceedings of KNASTIK Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati JURNAL PEMULIAAN TANAMAN HUTAN agriTECH Biology, Medicine, & Natural Product Chemistry Pro-Life Edumatsains Jurnal Pendidikan Biologi Jurnal Biodjati Biosaintifika: Journal of Biology & Biology Education Jurnal Inovasi Hasil Pengabdian Masyarakat (JIPEMAS) Jurnal Entomologi Indonesia Jurnal Pembelajaran dan Biologi Nukleus Sciscitatio : Journal for Biological Science Jurnal Agrohut
Aniek Prasetyaningsih
Program Studi Biologi, Fakultas Bioteknologi, Universitas Kristen Duta Wacana
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Education Engineering Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Public Health Other

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Sorghum (Sorghum bicolor L. Moench var. bioguma) Cookies (SoKis): Source of Antioxidant and Prebiotic Wiryawan, Dwiki Maycellino; Ariestanti, Catarina Aprilia; Prasetyaningsih, Aniek
agriTECH Vol 44, No 4 (2024)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/agritech.87733

Abstract

Sorghum bicolor L. Moench,  locally called “cantel,” is an underused local food in Indonesia, which contains resistant starch and has the potential to act as prebiotic. Several studies have also reported the role of its phytochemical compounds as antioxidants. Therefore, this study aims to determine the potential of sorghum as a source of antioxidants and prebiotic in cookies products. Sorghum was dried under various temperatures (40, 55, and 70 °C) for 4 h, ground into flour, and used in cookies production to replace wheat flour (WF) in various ratios of sorghum flour (SF):WF (0:100, 25:75, 50:50, 75:25, 100:0 % (w/w)). Phytochemical compounds were tested using the maceration method and qualitatively by assessing the color change and physical appearance of SF. Antioxidants activity was analyzed using the 1,1-diphenyl-2- picrylhydrazyl (DPPH) method, while nutritional content was determined with proximate analyses. The total plate count (TPC) method was carried out to assess the growth of Bifidobacterium longum, and organoleptic test (n = 30) was performed using 5 points-hedonic scale. SF with a drying temperature of 55˚C was used as an ingredient for making cookies based on its moisture content (4.24 ± 0.084) and antioxidant activity value (70.77 ± 2.91%). The analysis results showed the presence of flavonoid, alkaloid, tannin, and polyphenol compounds in SF. Cookies with SF:WF ration of 50:50 (w/w) (SoKis) showed the best acceptance based on organoleptic test compared to the other formulation with antioxidant activity of 36.18 ± 2.56%. In addition, soKis contained 2.715% water, 1.425% ash, 24.57% fat, 8.24% protein, 63.05% carbohydrate, 0.255% crude fiber and could support the growth of B. longum with a value of 2.46 × 10 8 CFU/mL. Based on these results, sorghum could be used and developed as a functional food ingredient.
PERSEPSI MAHASISWA PRODI BIOLOGI TENTANG IMPLEMENTASI MERDEKA BELAJAR KAMPUS MERDEKA DI SALAH SATU PERGURUAN TINGGI SWASTA INDONESIA Prakasita, Vinsa Cantya; Prasetyaningsih, Aniek; Prasetya, Heribertus Sigit
Jurnal Pendidikan Biologi Vol 15, No 2 (2024)
Publisher : Universitas Negeri Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17977/um052v15i2p180-186

Abstract

Abstrak. Merdeka Belajar-Kampus Merdeka (MBKM) merupakan kebijakan baru di bidang pendidikan di Indonesia. Pemerintah mengharapkan adanya peningkatan kompetensi lulusan, baik hard skills maupun soft skills serta sifat kapital intelektual yang kompetitif dan komperatif untuk menyambut globalisasi. Persepsi mahasiswa mengenai pemahaman dan kesiapan implementasi MBKM perlu diketahui sebagai masukan kepada pemangku kebijakan agar tujuan program dapat tercapai. Penelitian ini menggunakan pendekatan deskriptif-kuantitatif dan kuantitatif. Data diperoleh dari hasil Survei Implementasi MBKM 2021 yang dilakukan oleh Kementerian Pendidikan dan Kebudayaan Republik Indonesia terhadap mahasiswa Prodi Biologi di salah satu PTS Indonesia. Hasil analisis menunjukkan bahwa MBKM dapat menjadi solusi untuk meningkatkan hard skills dan soft skills mahasiswa. Bentuk kegiatan pembelajaran MBKM sesuai dengan kebutuhan lulusan Prodi Biologi di masa mendatang. Sebagai kesimpulan, pemahaman yang baik mengenai program MBKM dan dukungan dari pemerintah perguruan tinggi maupun orang tua akan mendukung terwujudnya tujuan dari program MBKM yaitu mencetak pemimpin masa depan yang unggul dan berkepribadian. Abstract. Freedom to Learn (MBKM) is a new policy of education in Indonesia. The government expects an increase in the competence of graduates, both hard skills and soft skills as well as the competitive and comparative nature of intellectual capital to face up globalization. Students' perceptions of understanding and readiness to implement MBKM need to be known as input to policy makers so that program objectives can be achieved. This research uses descriptive-quantitative and quantitative approaches. The data was obtained from the results of the 2021 MBKM Implementation Survey conducted by the Ministry of Education and Culture of the Republic of Indonesia on Biology Study Program students at one of the Indonesian private universities. The results of the analysis show that MBKM can be a solution to improve students' hard skills and soft skills. The form of MBKM learning activities is in accordance with the needs of future Biology Study Program graduates. In conclusion, a good understanding of the MBKM program and support from the university government and parents will support the realization of the objectives of the MBKM program, which is to produce future leaders who are superior and with good personalities.
Percontohan taman toga serta produksi jamu berbasis tanaman berkhasiat untuk peningkatan kesehatan dan ekonomi masyarakat Rahardjo, Djoko; Mularum, Seta Nurhayati; Madyaningrana, Kukuh; Ariestanti, Catarina Aprilia; Prasetyaningsih, Aniek; Widayanti, Nurlia
Jurnal Inovasi Hasil Pengabdian Masyarakat (JIPEMAS) Vol 5 No 2 (2022)
Publisher : University of Islam Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33474/jipemas.v5i2.14328

Abstract

Tanaman berkhasiat obat banyak dimanfaatkan oleh masyarakat di Indonesia secara turun-temurun untuk mendukung kesehatan. Inventarisasi dan pemanfaatan beragam tanaman obat mutlak dilakukan untuk pengembangan potensinya dalam menunjang kesehatan. Pemanfaatan jamu sebagai produk olahan tanaman obat mulai mendapatkan perhatian lebih dari pemerintah melalui institusi kesehatan. Pengabdian ini bertujuan untuk membuat percontohan taman tanaman obat keluarga (Toga) di Dusun Ngelosari, Kecamatan Piyungan, Kabupaten Bantul yang memadukan rintisan ekowisata lereng bukit dan pengolahan tanaman obat keluarga. Kegiatan pengabdian ini didasarkan pada analisis situasi dan permasalahan kesehatan di lingkup Puskesmas Piyungan. Berdasarkan identifikasi masalah dan analisis kebutuhan yang dilakukan bersama puskesmas dan kader kesehatan maka pendekatan pengembangan percontohan taman toga, pelatihan dan pendampingan pada kelompok wanita tani (KWT) dipilih sebagai pendekatan yang diharapkan mampu menyelesaikan permasalahan kesehatan dan ekonomi masyarakat. Program terealisir dalam bentuk persiapan dan observasi lokasi taman Toga, pengembangan taman Toga percontohan, pelatihan budidaya tanaman obat, pasca panen dan pengolahan, serta pengemasan dan pemasaran produk herbal. Kegiatan ini mempunyai hasil berupa Taman Toga Ngupoyo Sehat yang menjadi sumber bahan baku produk jamu yang dihasilkan oleh KWT setempat. Selain meningkatkan pendapatan KWT setempat, pelaksanaan program dapat dipergunakan sebagai model sinergisme pengembangan konservasi dan peningkatan kesehatan masyarakat.
Strategi Pengembangan Kawasan Ekowisata Pantai Jungwok, Desa Jepitu, Kecamatan Girisubo, Kabupaten Gunungkidul, Yogyakarta Soleman, Elisabet Suharti; Kisworo, Kisworo; Prasetyaningsih, Aniek
Jurnal Agrohut Vol. 14 No. 1 (2023): Agrohut
Publisher : Fakultas Pertanian Universitas Darussalam Ambon

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51135/agh.v14i1.167

Abstract

Pantai Jungwok merupakan sebagai salah satu objek ekowisata pantai yang terletak Daerah Istimewa Yogyakarta tepatnya di kabupaten Gunungkidul. Pantai ini memiliki potensi wisata yang sangat indah dengan ciri khas pasir putih dan bentuk pesisir pantai yang melengkung yang dapat memanjakan mata bagi wisatawan yang berkunjung ke Pantai Jungwok. Pengembangan Pantai Jungwok sebagai destinasi wisata belum dapat berjalan dengan baik karena berbagai permasalahan yang pasti berkaitan dengan kondisi internal dan eksternal yang terjadi di sekitar Pantai Jungwok. Pada penelitian ini dilakukan untuk merancang strategi untuk pengembangan Pantai Jungwok melalui analiss SWOT dengan merumuskan strategi untuk membandingkan faktor eksternal yaitu peluang (opprtunities) dan ancaman (threats) dengan faktor internal berupa kekuatan (strenghts) dan kelemahan (weaknesses). Data yang digunakan pada penelitian ini berupa data primer dan sekunder. Data primer yang didapatkan dari hasil observasi, wawancara, dan hasil dokumentasi, sedangkan untuk data sekunder didapatkan dari studi literatur yang terkait. Dari hasil penelitian, strategi yang dirancang yaitu mendorong pengembangan suatu kawasan wisata dalam membentuk para pemandu wisata, mengadakan pelatihan untuk pokdarwis, melakuan promosi yang maksimal dan yang menarik wisatawan untuk berkunjung, melengkapi dan meningkatkan kualitas sarana dan prasarana serta infrastruktur, membentuk kelompok atau lembaga dalam pengelolaan ekowisata, memperbanyak populasi vegetasi di sekitar pantai, Tetap menjaga biota laut jenis lobster dengan tidak merusak ekosistemnya sehingga tidak terancam kepunahan dan Pemeliharaan serta pengelolaan objek wisata yang bertanggung jawab dari masyarakat setempat. Untuk itu proses pengembangan potensi wisata pantai Jungwok memerlukan banyak dukungan baik dari pemerintah, masyarakat sekitar, serta dari wisatawan itu sendiri.
Antidiabetic Potential of Ethanol Extract from Moringa oleifera Leaves in Streptozotocin-Nicotinamide-Induced Female Mus musculus Prakasita, Vinsa Cantya; Theodora, Nadya Aprina; Susilowati, Enjelin Anjung; Aditiyarini, Dwi; Prasetyaningsih, Aniek
Jurnal Biodjati Vol 9 No 1 (2024): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v9i1.30699

Abstract

Type 2 diabetes mellitus (DM) is a chronic metabolic dis­order characterized by elevated blood glucose levels due to insulin resistance. Type 2 diabetes is considerably more prevalent than oth­er forms (85–90%). The risk of type 2 diabetes is higher in women (53.2%). There is an urgent requirement for better and more afforda­ble treatment options considering DM therapy is expensive and may have adverse health effects. The study's objective is to examine how acute toxicity, blood glucose levels, and body weight are affected by Moringa leaf ethanol extract (MLEE). Maceration was employed to eliminate the leaves of the moringa plant. Phytochemical screening was completed to assess the total flavonoid content and screen for alkaloids, flavonoids, phenolics, saponins, and tannins. Acute toxicity testing was performed following OECD guideline 423. Clinical symp­toms of acute toxicity were observed every 30 minutes for the first 24 hours post-treatment, followed by observations every 24 hours up to 14 days. The estimated LD50 range was determined. Streptozotocin/ nicotinamide-induced female Mus musculus was administered to eval­uate the antidiabetic potential of MLEE. Six groups of mice were uti­lized, which included a healthy control group (aquades not induced), a negative control group (induced aquades), a positive control group (induced glimepiride 0.8 mg/kg BW), and three treatment groups with varying dosages of MLEE (induced; 0, 100, and 150 mg/kg BW). A semi-auto chemical analyzer was employed on days 0 through 31 to determine blood glucose levels. An analytical digital balance was utilized to calculate the body weight. With a total flavonoid concen­tration of 20.75%, MLEE incorporated alkaloids, flavonoids, pheno­lics, saponins, and tannins. MLEE demonstrated a significant effect in lowering blood glucose levels at a dose of 100 mg/kg BW (P<0.05). A significant positive correlation has been identified between body weight and blood glucose levels (P<0.05).
Pengaruh Konsentrasi Air Kelapa dan BAP (6-Benzylaminopurin) terhadap Regenerasi In vitro Protokorm Dendrobium stratiotes x Dendrobium wulaiense Agustinus, Vania; Restiani, Ratih; Prasetyaningsih, Aniek
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 9, No 2 (2024): June 2024
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v9i2.7728

Abstract

Kultur in vitro merupakan salah satu solusi alternatif untuk mengatasi kesulitan perbanyakan anggrek melalui biji. Keberhasilan regenerasi protokorm anggrek dipengaruhi oleh penambahan ZPT dan air kelapa ke dalam medium. Oleh karena itu, penelitian ini bertujuan untuk menentukan pengaruh konsentrasi air kelapa dan BAP terhadap regenerasi protokorm Dendrobium hibrida. Penelitian menggunakan Rancangan Acak Lengkap dengan dua faktor yaitu konsentrasi air kelapa dan BAP. Paramater yang diamati meliputi persentase tunas, jumlah tunas, tinggi protokorm, dan persentase akar. Hasil menunjukkan bahwa penambahan air kelapa dan BAP ke dalam medium VW berpengaruh nyata terhadap tinggi dan persentase akar protokorm. Konsentrasi air kelapa (10%) tanpa penambahan BAP dapat meningkatkan persentase tunas, akar, jumlah tunas, dan tinggi protokorm Dendrobium hibrida. Hasil penelitian menunjukan bahwa penggunaan air kelapa secara tunggal maupun kombinasi dengan hormon sitokinin (BAP) signifikan dalam mempercepat regenerasi protokorm Dendrobium hibrida. Ini merupakan penelitian pertama yang melaporkan penggunaan air kelapa dan BAP pada regenerasi protokorm hasil persilangan Dendrobium stratiotes x Dendrobium wulaiense.
Pengaruh pemberian sampah organik terhadap pertumbuhan dan kandungan astaxanthin pada pupa black soldier fly (Hermetia illucens (Linnaeus)) Sidauruk, Arkhey; Prasetyaningsih, Aniek; Madyaningrana, Kukuh
Jurnal Entomologi Indonesia Vol 21 No 1 (2024): March
Publisher : Perhimpunan Entomologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5994/jei.21.1.23

Abstract

Black soldier flies (Hermetia illucens (Linnaeus)) or BSF are insects that process organic waste, with larvae rich in nutrients and potential as an alternative protein source for food and feed. Astaxanthin, an antioxidant found in plants, algae, and crustaceans, is also assumed to be present in insects like BSF. This study examined the impact of feeding different organic wastes on BSF larval growth and astaxanthin content in its pupae. The experimental design involved four treatments: fruit and vegetable scraps (BS), tofu pulp (AT), rice leftovers (N), and a mix of fruit and vegetable scraps, tofu pulp and rice left-over (C), with animal feed T51 as control. Each treatment was repeated three times. Growth parameters of BSF such as weight, length, and larval characteristics, along with the chemical content of pupae, including astaxanthin, were measured. Pupae were extracted using a triphasic maceration method with n-heptane, ethyl acetate, acetonitrile, butanol-l, and distilled water. Compound identification was done using terpenoid qualitative tests, thin layer chromatography (TLC), and liquid chromatography-mass spectrometry (LC-MS). The findings revealed that fruit and vegetable scraps (BS) significantly increased larval body size, with a weight of 0.09 ± 0.04 grams/larvae and a length of 1.49 ± 0.31 cm/larvae. LC-MS analysis detected lutein derivatives, anisodamine, resveratrol derivatives, dehydroarginine, and astaxanthin derivatives with a molecular mass of 597.23 m/z in BSF larvae fed on fruit and vegetable scraps.
Effect of BAP and 2,4-D Combination on the Callus Induction of Robusta Coffee (Coffea canephora Pierre ex A.Froehner) Leaf Explants Wijaya, Yovita Angeline; Restiani, Ratih; Prasetyaningsih, Aniek
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 11, No 4: Jurnal Pembelajaran Dan Biologi Nukleus December 2025
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v11i4.8338

Abstract

Background: Sustainable production of Robusta coffee (Coffea canephora) is constrained by the limited availability of high-quality planting materials, while information regarding the optimal auxin–cytokinin combinations for initiating callus cultures remains insufficient. Accordingly, this study was conducted to examine the effects of combined applications of benzyl amino purine (BAP) and 2,4-dichlorophenoxyacetic acid (2,4-D) on callus induction and subsequent growth derived from young leaf explants of Robusta coffee. Methodology: Explant cultures were established on Murashige and Skoog (MS) medium enriched with different concentrations of 2,4-D (1, 1.5, and 2 ppm) in combination with BAP (1.5, 2, and 2.5 ppm), arranged in a completely randomized design with three replications. Data collection was conducted throughout a 30-day incubation period and included measurements of callus initiation time (days), callus formation percentage, callus growth intensity, and callus morphological features. The resulting data were analyzed statistically using analysis of variance (ANOVA), followed by the Kruskal–Wallis test. Findings: Result describe that the treatment combining 2,4-D at 1 ppm with BAP at 1.5 ppm produced the most favorable response, as indicated by the shortest callus initiation period (7.33 DAP). In contrast, the application of 2,4-D at 1 ppm in combination with BAP at 2.5 ppm achieved a 100% callus induction rate and the highest callus growth intensity (2 on a 5-point scale), along with a compact callus exhibiting a yellowish-green coloration. Overall, these results highlight the critical influence of auxin–cytokinin equilibrium in enhancing callus induction in Robusta coffee and underscore its significance as a foundational reference for establishing an efficient in vitro propagation strategy. Contribution: In general, this study contributes to providing important technical information supporting the large-scale and sustainable production of high-quality Robusta coffee seedlings
Co-Authors Adonia Felma Mosse Agustinus, Vania Angelia Astria Ariestanti, Catarina Aprilia AYPBC Widyatmoko Catarina Aprilia Ariestanti Devi Sonti Sibarani Djima, Elza Gitaa Eirene Djima Djoko Rahardjo Djoko Rahardjo Dwi Aditiyarini Dwi Adityarini Elsih Dian Ariningsih Kania Apenta Olisvelos Kisworo Kisworo, Kisworo Kukuh Madyaningrana Lavenia, Jovita Maria Grasela Kase Meilani Apra Meyga Semarayani, Cokorda Istri Mularum, Seta Nurhayati Nurlia Widayanti Prasetya, Heribertus Sigit Putri Octaviana Ginting, Naomi Bastanta Ratih Restiani Santoso, Julyana Dika Sekar, Astrid Ayu Seta Nurhayati Mularum Setya Harianto Sidauruk, Arkhey Soleman, Elisabet Suharti Susilowati, Enjelin Anjung Tara Inastu Kandarp Theodora, Nadya Aprina Tiffany, Yollanda Vinsa Cantya Prakasita Widayanti, Nurlia Wijaya, Yovita Angeline Willy Sudiarto Raharjo Wiryawan, Dwiki Maycellino Wulan Krista Waty