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All Journal AGRISAINS Biotika: Jurnal Ilmiah Biologi AL KAUNIYAH Proceedings of KNASTIK Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati JURNAL PEMULIAAN TANAMAN HUTAN agriTECH Biology, Medicine, & Natural Product Chemistry Pro-Life Edumatsains Jurnal Pendidikan Biologi Jurnal Biodjati Biosaintifika: Journal of Biology & Biology Education Jurnal Inovasi Hasil Pengabdian Masyarakat (JIPEMAS) Jurnal Entomologi Indonesia Jurnal Pembelajaran dan Biologi Nukleus Sciscitatio : Journal for Biological Science Jurnal Agrohut
Aniek Prasetyaningsih
Program Studi Biologi, Fakultas Bioteknologi, Universitas Kristen Duta Wacana
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Education Engineering Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Public Health Other

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UJI EFEKTIVITAS SALEP KULIT BATANG KAPUK RANDU (Ceiba Pentandra) SEBAGAI OBAT ANTI-INFLAMASI Setya Harianto; Aniek Prasetyaningsih; Vinsa Cantya Prakasita
EduMatSains : Jurnal Pendidikan, Matematika dan Sains Vol 6 No 1 (2021): Juli
Publisher : Fakultas Keguruan dan Ilmu Pendidikan, Universitas Kristen Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/edumatsains.v6i1.2799

Abstract

Inflammation is protective response of vascular tissue to various harmful stimuli that characterized by pain, redness, and swelling. Empirically, stem bark of Ceiba pentandra has various chemical compounds that nutritious for health and have potential to be used as anti-inflammatory. This research will provide information the benefit of C. pentandra stem bark especially as anti-inflammatory agents and it’s correlation with the compounds. Maceration method with 70% ethanol was used in extraction and the yield was 75,54%. In phytochemical test, it was identified in crude extract contained flavonoids, tannins, saponins, steroids, β-sitosterol, 1,2-benzendiol, quinic acid, and octadec-9-enoic acid. On extract and ointment products C. pentandra has antioxidants activity with IC50 value of extract was 52,64 ppm and IC50 value of ointment products was 117,64 ppm. This research is included RAL experimental which was carried out on male mice that divided into 5 groups: negative control (ointment base), positive control (Betametasone valerate 0,1%), and 50%, 75%, 100% of C. pentandra ointment. The ointment was applied after the mice were induced by carrageenan, measured with the parameter of skinfold thickness of mice’s back every hour for 6 hours of observation. The result showed C. pentandra stem bark ointments has anti-inflammatory effect which %PI value in the 50%, 75%, 100% were respectively 10,85%; 18,99%; 30,84%.
Percontohan taman toga serta produksi jamu berbasis tanaman berkhasiat untuk peningkatan kesehatan dan ekonomi masyarakat Djoko Rahardjo; Seta Nurhayati Mularum; Kukuh Madyaningrana; Catarina Aprilia Ariestanti; Aniek Prasetyaningsih; Nurlia Widayanti
Jurnal Inovasi Hasil Pengabdian Masyarakat (JIPEMAS) Vol 5, No 2 (2022): Jurnal Inovasi Hasil Pengabdian Masyarakat (JIPEMAS)
Publisher : University of Islam Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33474/jipemas.v5i2.14328

Abstract

Tanaman berkhasiat obat banyak dimanfaatkan oleh masyarakat di Indonesia secara turun-temurun untuk mendukung kesehatan. Inventarisasi dan pemanfaatan beragam tanaman obat mutlak dilakukan untuk pengembangan potensinya dalam menunjang kesehatan. Pemanfaatan jamu sebagai produk olahan tanaman obat mulai mendapatkan perhatian lebih dari pemerintah melalui institusi kesehatan. Pengabdian ini bertujuan untuk membuat percontohan taman tanaman obat keluarga (Toga) di Dusun Ngelosari, Kecamatan Piyungan, Kabupaten Bantul yang memadukan rintisan ekowisata lereng bukit dan pengolahan tanaman obat keluarga. Kegiatan pengabdian ini didasarkan pada analisis situasi dan permasalahan kesehatan di lingkup Puskesmas Piyungan. Berdasarkan identifikasi masalah dan analisis kebutuhan yang dilakukan bersama puskesmas dan kader kesehatan maka pendekatan pengembangan percontohan taman toga, pelatihan dan pendampingan pada kelompok wanita tani (KWT) dipilih sebagai pendekatan yang diharapkan mampu menyelesaikan permasalahan kesehatan dan ekonomi masyarakat. Program terealisir dalam bentuk persiapan dan observasi lokasi taman Toga, pengembangan taman Toga percontohan, pelatihan budidaya tanaman obat, pasca panen dan pengolahan, serta pengemasan dan pemasaran produk herbal. Kegiatan ini mempunyai hasil berupa Taman Toga Ngupoyo Sehat yang menjadi sumber bahan baku produk jamu yang dihasilkan oleh KWT setempat. Selain meningkatkan pendapatan KWT setempat, pelaksanaan program dapat dipergunakan sebagai model sinergisme pengembangan konservasi dan peningkatan kesehatan masyarakat.
Antioxidant and Antibacterial Activity of Pomegranate Extract (Punica granatum L.) in Lip Balm Formulation Maria Grasela Kase; Aniek Prasetyaningsih; Dwi Aditiyarini
Biology, Medicine, & Natural Product Chemistry Vol 12, No 1 (2023)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2023.121.109-117

Abstract

Lips are a part of the face that does not have hair follicles making them easily dry and crack. Lipbalm is a beauty product that could be used to solve this problem by increasing lip moisture. However, the synthetic ingredient in commercial lip balm can have side effects on the body in the long term. Pomegranate fruit is one of the natural ingredients containing anthocyanin which can be used as a natural dye, antioxidant, and antibacterial. Therefore, this study was performed to study the potency of pomegranates as natural dyes, antioxidants, and antibacterial in lip balm. Pure pomegranate juice was obtained through squeeze step. Lip balm was prepared in several concentrations of pomegranate juice which were 0%, 12.5%, 18.75%, and 25%. Phytochemical screening shows the content of anthocyanin, saponin, tannin, and flavonoid. In this study, pomegranate juice has low antioxidant activity with IC50 449 ppm. Lip balm formula with 18.75% and 25% of pomegranate juice can inhibit the growth of the bacteria Staphylococcus aureus.
Potensi Minyak Sereh Wangi (Cymbopogon nardus) sebagai Antibakteri terhadap Propionibacterium acnes dan Staphylococcus aureus pada Sediaan Gel Antijerawat Kania Apenta Olisvelos; Dwi Aditiyarini; Aniek Prasetyaningsih
Jurnal Pro-Life Vol. 10 No. 1 (2023): Maret
Publisher : Program Studi Pendidikan Biologi Fakultas Keguruan dan Ilmu Pendidikan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/jpvol6Iss2pp102

Abstract

Acne can be caused by the bacteria Propionibacterium acnes and Staphylococcus aureus. One way to treat acne is to use antibiotics. However, this can create resistance. In this study, citronella oil will be formulated into an anti-acne gel preparation with various concentrations of 10%, 15% and 20%. The study began with distillation of citronella oil and analysis and identification of compounds. Then the gel was made by mixing citronella oil in the HPMC gel base and then the gel preparation was tested for antibacterial activity by the disc diffusion method and physical preparation tests included organoleptic tests, pH measurements, viscosity tests, homogeneity tests, dispersion tests, gel stability tests. Based on the results of compound analysis using GC-MS, citronella oil contains geraniol (55.05%). Of the three formulations, the preparation that has good gel physical properties based on its physical properties is F2 with 15% citronella oil content. In the antibacterial test, the results showed that citronella oil gel had the ability to inhibit Propionibacterium acnes and Staphylococcus aureus with the most optimal formulation being F2 with an average inhibition of 1.5 cm.
Potensi Ekstrak Kulit Buah Pepaya (Carica papaya L.) Untuk Isolasi Kolagen Cumi-cumi (Loligo sp.) Sebagai Penyembuhan Luka Kulit Mencit (Mus musculus) Angelia Astria; Aniek Prasetyaningsih; Vinsa Cantya Prakasita
Al-Kauniyah: Jurnal Biologi Vol 16, No 2 (2023): AL-KAUNIYAH JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v16i2.17629

Abstract

AbstrakKulit buah pepaya merupakan bagian dari buah pepaya yang tidak dikonsumsi dan mengandung enzim papain yang dapat digunakan sebagai pengganti papain murni untuk mengekstraksi kolagen. Tentakel cumi-cumi merupakan salah satu dari bahan baku marine collagen. Ekstraksi kolagen dari tentakel cumi-cumi menggunakan metode maserasi menggunakan CH3COOH dan penambahan ekstrak kulit buah pepaya pada konsentrasi 5, 10, 15, dan 20%. Hasil menunjukkan bahwa konsentrasi 15% ekstrak kulit buah pepaya memberikan rendemen yang terbaik yaitu 19,2% (w). Kolagen yang dihasilkan memenuhi kriteria Badan Standardisasi Nasional (BSN) (2014) pada jumlah mikrobia dan total coliform tetapi belum memenuhi pada kadar protein, pH, dan kadar air. Pada uji preklinis digunakan kolagen hasil ekstraksi dengan penambahan 20% ekstrak kulit buah pepaya yang memenuhi kriteria BSN dengan kadar protein terbanyak. Serum kolagen dibuat menjadi tiga konsentrasi, yaitu 5, 10, dan 20 mg/mL. Hasil pengujian preklinis menunjukkan bahwa kelompok perlakuan kolagen 20 mg/mL memiliki presentase penutupan luka kulit mencit terbaik sebesar 84,61%, dari hasil pengujian statistik menunjukkan tidak ada perbedaan signifikan dengan kelompok perlakuan kontrol positif.AbstractThe papaya peel, which is a part of papaya not typically consumed, contains the papain enzyme that can be used as a substitute for pure papain to extract the collagen. Squid tentacles are one of the mainsources of marine collagen. The extraction of collagen from squid tentacles was conducted using a maceration method with CH3CHOOH solvent, and the papaya peel extract was added at concentrations of 5, 10, 15, and 20%, respectively. The extraction process showed that the addition of 15% papaya peel extract provided the best result of 19.2% (bb). The collagen produced in this experiment met the BSN criteria for total microbes and total coliforms. However, it did not yet comply with the BSN criteria for protein content, pH, and water levels. The preclinical testing utilized the extracted collagen and the papaya peel extract with a 20% concentration, which met the BSN standards with the highest protein level. The serum collagen was divided into three concentrations: 5 mg/ml, 10 mg/ml, and 20 mg/ml. Preclinical studies showed that the treatment group receiving 20 mg/mL collagen exhibited the highest percentage of skin wound healing at 84.61%, and the statistical test found no significant difference compared to the positive control treatment group.
Browning Prevention Method in Kepel (Stelechocarpus burahol (Blume) Hook.f. & Thomson) in Vitro Culture Devi Sonti Sibarani; Ratih Restiani; Aniek Prasetyaningsih
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS (JPBN) Vol 10, No 1 (2024): Jurnal Pembelajaran Dan Biologi Nukleus March 2024
Publisher : Fakultas Keguruan dan Ilmu Pendidikan Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v10i1.5464

Abstract

Browning of explants is a common problem in Kepel (Stelechocarpus burahol (Blume) Hook.f. & Thomson) in vitro culture, resulting in low explant in vitro regeneration of Kepel. Using nodal explant, the effect of explant immersion in ascorbic acid, the addition of ascorbic acid to the media, and dark and light incubation conditions were investigated. The browning prevention method was selected based on the delayed browning appearance time, the lowest broning intensity, and the highest percentage of callus. This present study used a completely randomized design with treatment variations: immersion explant in ascorbic acid 100 mg/L, addition of ascorbic acid 100 and 200 mg/L to the media, addition of ascorbic acid 100 and 200 mg/L and activated charcoal 1 g/L to the media, and incubation in dark and light conditions for 28 days. The results showed that the combination of the addition of ascorbic acid 200 mg/L to MS media and incubation in dark conditions were effective browning prevention methods in inhibiting browning appearance time (5 DAP), significantly reducing browning intensity (0.3), and increasing callus growth (100%) of kepel node explants during 28 days of culture. The results of this study are useful in establishing protocols for in vitro culture of Kepel plants, especially at the initiation stage, and can be expected to support the successful conservation of Kepel plants through in vitro propagation. Keywords: Ascorbic acid, Activated Charcoal, Browning, In Vitro Culture, Stelechocarpus burahol
INDUKSI KALUS DARI EKSPLAN NODUS Stelecocharpus burahol (Blume) Hook. f & Thomson SEBAGAI UPAYA KONSERVASI IN VITRO Sekar, Astrid Ayu; Restiani, Ratih; Prasetyaningsih, Aniek
BIOTIKA Jurnal Ilmiah Biologi Vol 21, No 1 (2023): BIOTIKA JUNI 2023
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/biotika.v21i1.42869

Abstract

Tanaman Kepel (Stelechocarpus burahol (Blume) Hook. f. & Thomson) merupakan salah satu jenis tanaman asli Indonesia yang mengandung metabolit sekunder dan potensial sebagai antioksidan, antibakteri, antifungi, anti inflamasi, dan anti implantasi. Namun, saat ini tanaman kepel berstatus conservation dependent, sehingga diperlukan perbanyakan tanaman kepel melalui kultur in vitro. Penelitian ini bertujuan untuk menghasilkan metode perbanyakan tanaman kepel secara in vitro melalui tahap induksi kalus dengan mengoptimasi kombinasi dan konsentrasi BAP (Benzylaminopurin) dan IAA (Indole-3-acetic acid). Penelitian ini menggunakan desain Rancangan Acak Lengkap (RAL) satu faktor dengan kombinasi konsentrasi BAP dan IAA (0,1,2,5, dan 5 mgL-1 ) sebanyak 16 perlakuan masing-masing 3 ulangan. Eskplan nodus yang ditanam dalam medium MS dengan penambahan BAP dan IAA dikultur pada suhu 25 ± 2 0C, kondisi terang 24 jam dengan intensitas cahaya 3000 flux selama 30 hari. Pengamatan waktu induksi kalus, persentase pertumbuhan kalus dan intensitas kalus dilakukan setiap minggu selama 28 hari masa tanam. Data yang diperoleh dianalisis menggunakan ANOVA dan tidak dilanjutkan uji Duncan karena hasil yang diperoleh tidak berbeda signifikan (p ≥0,05). Hasil menunjukkan bahwa penambahan 1 mgL-1 BAP dan 5 mgL-1 IAAmenghasilkan waktu induksi tercepat yaitu 4,67 ± 1,15 hari, sedangkan medium MS dengan penambahan 5 mgL-1 BAP dan 2,5 mgL-1 IAAmerupakan kombinasi konsentrasi terbaik dalam pembentukan kalus (100%) dan intensitas kalus sebesar 0,57 ± 0,34 dengan tekstur remah berwarna kehijauan. Hasil penelitian ini dapat menjadi informasi penting dalam upaya konservasi tanaman kepel secara in vitro.
Pengaruh Konsentrasi Air Kelapa dan BAP (6-Benzylaminopurin) terhadap Regenerasi In vitro Protokorm Dendrobium stratiotes x Dendrobium wulaiense Agustinus, Vania; Restiani, Ratih; Prasetyaningsih, Aniek
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 9, No 2 (2024): June 2024
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v9i2.7728

Abstract

Kultur in vitro merupakan salah satu solusi alternatif untuk mengatasi kesulitan perbanyakan anggrek melalui biji. Keberhasilan regenerasi protokorm anggrek dipengaruhi oleh penambahan ZPT dan air kelapa ke dalam medium. Oleh karena itu, penelitian ini bertujuan untuk menentukan pengaruh konsentrasi air kelapa dan BAP terhadap regenerasi protokorm Dendrobium hibrida. Penelitian menggunakan Rancangan Acak Lengkap dengan dua faktor yaitu konsentrasi air kelapa dan BAP. Paramater yang diamati meliputi persentase tunas, jumlah tunas, tinggi protokorm, dan persentase akar. Hasil menunjukkan bahwa penambahan air kelapa dan BAP ke dalam medium VW berpengaruh nyata terhadap tinggi dan persentase akar protokorm. Konsentrasi air kelapa (10%) tanpa penambahan BAP dapat meningkatkan persentase tunas, akar, jumlah tunas, dan tinggi protokorm Dendrobium hibrida. Hasil penelitian menunjukan bahwa penggunaan air kelapa secara tunggal maupun kombinasi dengan hormon sitokinin (BAP) signifikan dalam mempercepat regenerasi protokorm Dendrobium hibrida. Ini merupakan penelitian pertama yang melaporkan penggunaan air kelapa dan BAP pada regenerasi protokorm hasil persilangan Dendrobium stratiotes x Dendrobium wulaiense.
Effect of Ascorbic Acid, Activated Charcoal and Dark Incubation on Browning Intensity of Saurauia bracteosa In Vitro Culture Putri Octaviana Ginting, Naomi Bastanta; Restiani, Ratih; Prasetyaningsih, Aniek; Meyga Semarayani, Cokorda Istri
Biosaintifika: Journal of Biology & Biology Education Vol 15, No 3 (2023): December 2023
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v15i3.48439

Abstract

Saurauia bracteosa, also known as Pirdot, is an endemic woody plant of the Actinidiaceae family with medicinal properties. Its population decline in nature has led to a vulnerable status, prompting conservation efforts, such as in vitro culture. Browning poses a significant challenge in the woody plant in vitro culture. Browning inhibitors such as ascorbic acid, activated charcoal and dark incubation can be used to address this issue. This study aims to determine the effect of ascorbic acid, activated charcoal and incubation conditions on browning inhibition of S.bracteosa. The study employed a Completely Randomized Design with 12 treatments on leaf explants, repeated five times. Observations, including the time of browning formation, percentage of browning, intensity of browning and percentage of live explant were carried out for 30 days and analyzed using ANOVA followed by DMRT. The results showed that the addition of activated charcoal in MS media with dark incubation delayed the browning formation time (12.40 DAI) while the adding ascorbic acid in MS media and dark incubation resulted in the lowest browning intensity (0.22). These research findings can serve as a foundational protocol for browning prevention, supporting the successful in vitro conservation of S. bracteosa.
Pengaruh pemberian sampah organik terhadap pertumbuhan dan kandungan astaxanthin pada pupa black soldier fly (Hermetia illucens (Linnaeus)) Sidauruk, Arkhey; Prasetyaningsih, Aniek; Madyaningrana, Kukuh
Jurnal Entomologi Indonesia Vol 21 No 1 (2024): March
Publisher : Perhimpunan Entomologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5994/jei.21.1.23

Abstract

Black soldier flies (Hermetia illucens (Linnaeus)) or BSF are insects that process organic waste, with larvae rich in nutrients and potential as an alternative protein source for food and feed. Astaxanthin, an antioxidant found in plants, algae, and crustaceans, is also assumed to be present in insects like BSF. This study examined the impact of feeding different organic wastes on BSF larval growth and astaxanthin content in its pupae. The experimental design involved four treatments: fruit and vegetable scraps (BS), tofu pulp (AT), rice leftovers (N), and a mix of fruit and vegetable scraps, tofu pulp and rice left-over (C), with animal feed T51 as control. Each treatment was repeated three times. Growth parameters of BSF such as weight, length, and larval characteristics, along with the chemical content of pupae, including astaxanthin, were measured. Pupae were extracted using a triphasic maceration method with n-heptane, ethyl acetate, acetonitrile, butanol-l, and distilled water. Compound identification was done using terpenoid qualitative tests, thin layer chromatography (TLC), and liquid chromatography-mass spectrometry (LC-MS). The findings revealed that fruit and vegetable scraps (BS) significantly increased larval body size, with a weight of 0.09 ± 0.04 grams/larvae and a length of 1.49 ± 0.31 cm/larvae. LC-MS analysis detected lutein derivatives, anisodamine, resveratrol derivatives, dehydroarginine, and astaxanthin derivatives with a molecular mass of 597.23 m/z in BSF larvae fed on fruit and vegetable scraps.
Co-Authors Adonia Felma Mosse Agustinus, Vania Angelia Astria Ariestanti, Catarina Aprilia AYPBC Widyatmoko Catarina Aprilia Ariestanti Devi Sonti Sibarani Djima, Elza Gitaa Eirene Djima Djoko Rahardjo Djoko Rahardjo Dwi Aditiyarini Dwi Adityarini Elsih Dian Ariningsih Kania Apenta Olisvelos Kisworo Kisworo, Kisworo Kukuh Madyaningrana Lavenia, Jovita Maria Grasela Kase Meilani Apra Meyga Semarayani, Cokorda Istri Mularum, Seta Nurhayati Nurlia Widayanti Prasetya, Heribertus Sigit Putri Octaviana Ginting, Naomi Bastanta Ratih Restiani Santoso, Julyana Dika Sekar, Astrid Ayu Seta Nurhayati Mularum Setya Harianto Sidauruk, Arkhey Soleman, Elisabet Suharti Susilowati, Enjelin Anjung Tara Inastu Kandarp Theodora, Nadya Aprina Tiffany, Yollanda Vinsa Cantya Prakasita Widayanti, Nurlia Willy Sudiarto Raharjo Wiryawan, Dwiki Maycellino Wulan Krista Waty