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All Journal Buletin Peternakan Jurnal Sain Peternakan Indonesia Animal Production : Indonesian Journal of Animal Production Jurnal Peternakan Nusantara ANGON: Journal of Animal Science and Technology Animal Production
Caribu Hadi Prayitno
Universitas Jenderal Soedirman
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Environmental Science Immunology & microbiology Veterinary Other

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Journal : Animal Production : Indonesian Journal of Animal Production

 1 
The Cellulolytic Activity And Volatile Fatty Acid Product Of Rumen Bacteria Of Buffalo And Cattle On Rice Straw, Elephant Grass, and Sesbania Leaves Substrates Prayitno, Caribu Hadi; Hidayat, Nur
ANIMAL PRODUCTION Vol 1, No 1 (1999): January
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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Abstract

Experiment on The Cellulolytic Activity and Volatile Fatty Acid Product of Rumen Bacteria of Buffalo and Cattle on Rice Straw, Elephant Grass, and Sesbania Leaves Substrates had been conducted at Feedstuff Laboratory of Animal Science Soedirman University. The basic design  that was used in this experiment was Completely Randomized Design (CRD) with factorial pattern of 6 x 3, three replications. The bacteria isolate as the factors were cellulolytic rumen bacteria isolate of buffalo (A1, A2, and A3) and cattle (A4, A5 and A6) while the substrates (second factor)  were NDF rice straw (S1), elephant grass (S2), and sesbania leaves (S3) Cell walls. The result of this experiment showed that the interaction between bacteria isolate and substrate  type were significant on pH, NDF digestibility, cellulase activity, pH was  6.28 until 6.43.  The NDF digestibility range was 12.27 until 55.61 percent. The lowers of cellulase activity was 5.11 IU/ml and the higher was 24.47 IU/ml. The range of acetic acid yield was 63.37 to 307.467 mg/100 ml. Range of  propionic production was 15.17 to 352.20 mg/ 100 ml. The production of butiric acid was 8.77 to 40.87 mg/ 100 ml. The cellulase activity  of cellulolytic rumen bacteria of buffalo was higher than cattle, and also their effect on NDF digestibility of rice straw, elephant grass, and sesbania leaves cell walls. The A3 of cellulolytic rumen bacteria isolate of  buffalo changed cell walls substrat to volatile fatty  acid was more effective than cattle, especially on cell elephant grass. Propionic and butiric  acid that was produced by cellulolytic rumen bacteria isolate of buffalo more higher than cattle (Animal Production 1 (1) : 1-9 (1999)Key Words: Cellulolytic, VFA, Rumen Bacteria, Buffalo, Cattle.
The Diversity and Productivity of Indigenous Forage in Former Limestone Mining Quarry in Karst Mountain of Southern Gombong, Central Java Indonesia Sarwanto, Doso; Prayitno, Caribu Hadi
ANIMAL PRODUCTION Vol 17, No 2 (2015): May
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (785.478 KB) | DOI: 10.20884/1.anprod.2015.17.2.520

Abstract

Indonesia is a country that has a lot of limestone mountains, covering 15.4 million hectares. Limestone mountains have strategic functions as limestone is used as building materials and as raw material in cement industry. Therefore, limestone mining quarry in various areas of limestone mountains in Indonesia is increasingly widespread. The biggest negative impact of limestone mining is the formed open land which is abandoned and unutilized. Changes in the ecosystem will lead to the reduced levels of diversity and productivity of indigenous forage which will ultimately reduce the performance and development of ruminants livestock kept by farmers in the mountainous region of limestone. This study aims to determine the diversity and productivity of indigenous forage on former limestone mining quarry in limestone mountains of southern Gombong. The research was conducted through survey by identifying and measuring the forage production of sample plots assigned purposively. Location of the study was divided into three categories, mild, moderate and heavy mining. Results showed that soil fertility levels in open fields of former limestone mining in southern Gombong mountains are low with total N content of 0.049 - 0.141%, total P2O5 of 0.067 - 0.133% and total K2O of 0.086 - 0.100%. The diversity of indigenous forage on mild mining was more diverse than that of moderate and heavy mining, i.e. 13 species comprising 7 grass species, 2 legumes species, and 4 species of shrubs. The most dominant species in all mining categories are Cynodon dactylon, Imperata cylindrica, Ageratum conyzoides and Mikania micrantha. The results also showed that in the open land of mild mining had the highest production of fresh and dry matter compared to that of moderate and severe mining
Utilization of Pseudomonas fluorescens as Antimicrobial agent on Lipolysis Inhibitition of Rice Bran Widiyastuti, Titin; Prayitno, Caribu Hadi; Munasik, Munasik
ANIMAL PRODUCTION Vol 4, No 2 (2002): May
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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Abstract

Feedstuff was limited by fat content, certainly, if the material feedstuff was storage on long time periods. Fat was oxidized or hydrolyzed, which was decreased nutritional quality. A Research on Inhibited of  Lypolysis Rice Bran with anti-microbial compound of Pseudomonas fluorescens was conducted during ten month. The research used experiment methods, with Randomized Completely Block Design, storage as block and concentration of anti-mikrobial compound as treatment. Fat and polyunsaturated fatty acids content during storage was observed. Result of experiment showed anti-microbial compound inhibited oxidize and hydrolyze process of fat rice bran during six month storage. (Animal Production 4(2): 89-93 (2002) 
Supplementation of Heit-Chrose into Dairy Cow Feed Improves in Vitro Rumen Fermentation Prayitno, Caribu Hadi
ANIMAL PRODUCTION Vol 17, No 2 (2015): May
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (586.746 KB) | DOI: 10.20884/1.anprod.2015.17.2.500

Abstract

The objective of the study was to evaluate the effect of Heit-Chrose (HC) supplementation using in vitro method on ruminal fermentation of dairy cattle. HC is a feed supplement containing allicin, saponin and organic minerals ( Se , Cr and Zn).  This research was conducted using completely randomized design, with 6 treatments and 4 replications. The treatments were : 1). C = dairy cattle feed (CP 15.38%, CF 23.38%, TDN 61.26%); 2). HC-0 = C+  organic minerals (0.3 ppm Se + 0.15 ppm Cr + 40 ppm Zinc-lysinat) + 0 ppm of HC; 3). HC-15= C + 15 ppm HC; 4).HC-30 =C+ 30 ppm of HC; 5). HC-45 =C+ 45 ppm of  HC; 6). HC-60 =C  + 60 ppm of HC. Data obtained were analyzed using analysis of variance of SPSS program. HC supplementation increased the DMD, OMD, VFA, but reduced  total gas,  methane and  protozoa count.  HC supplementation greater than  30 ppm  did not further improve ruminal fermentation. Supplementation at 30 ppm of  HC to dairy cow feed was the appropriate level to improve the efficiency of rumen fermentation. 
The Efficacy of Methanol Extract of Garlic (Allium sativum) to Improve Rumen Fermentation Products Prayitno, Caribu Hadi; Hidayat, N
ANIMAL PRODUCTION Vol 15, No 1 (2013): January
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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Abstract

Abstract.  This study was aimed to evaluate the effect of the increase levels of garlic extract (Allium sativum, As) in beef cattle feed on rumen fermentation products and microbial populations. The materials used  were rumen fluid and control feed (40 % fermented rice straw:60% concentrate). The research method was experimental using Randomized Complete Block Design (RCBD) applying 7 treatments which were repeated 3 times. The tested feed treatments were S0 (control feed), S1 (S0 + 1.5 ppm of Cr, S2 (S0 + 250 ppm of As extract), S3 (S1 + 250 ppm of As extract), S4 (S1 + 500 ppm of As extract), S5 (S1 +  750 ppm of As extract) and, S6 (S1  + 1000 ppm of As extract). The data were analyzed using analysis of variance and followed by Honestly Significant Difference test. The results showed that the treatment effects on total bacteria, protozoa population, total gas and methane were highly significant but had no effect on dry matter digestibilityDMD and organic matter digestibility OMD and production of VFA. The treatments increased total bacteria but decreased total gas and methane. The best result was achieved on treatment S6 (supplementation of As at the level of 1000 ppm in the feed) of which the feed with adequate Cr will result in the most efficient of fermentation.Keywords: Extract of Allium sativum, organic Cr, microbial population, rumen fermentation, beef cattleAbstrak. Penelitian ini bertujuan untuk mengevaluasi pengaruh dan level penambahan ekstrak  bawang putih (Allium sativum, As) dalam pakan  sapi potong terhadap  produk fermentasi dan populasi mikroba rumen. Materi yang digunakan dalam penelitian adalah cairan rumen sapi potong dan pakan kontrol (jerami fermentasi :  konsentrat; 40:60%). Metode penelitian adalah eksperimental menggunakan Rancangan Acak Kelompok (RAK) 7 perlakuan, setiap perlakuan diulang 3 kali. Pakan perlakuan yang diujicobakan pada sapi potong yaitu S0: pakan kontrol, S1: S0 + 1,5 ppm Cr, S2: S0 + 250 ppm ekstrak As, S3: S1 + 250 ppm ekstrak As, S4: S1 + 500 ppm ekstrak As, S5: S1 + 750 ppm ekstrak As, S6: S1 + 1000 ppm ekstrak As. Data dianalisis menggunakan analisis variansi dilanjutkan uji Beda Nyata Jujur. Hasil penelitian menunjukkan  perlakuan  berpengaruh terhadap total bakteri, populasi protozoa,  gas total dan metan tetapi  tidak berpengaruh terhadap kecernaan bahan kering dan bahan organik serta produksi VFA.  Perlakuan mampu meningkatkan total bakteri serta menurunkan gas total dan metan. Hasil terbaik, pada pakan perlakuan S6 yaitu suplementasi ekstrak As pada taraf 1000 ppm pada pakan yang tercukupi Cr organik menghasilkan efisiensi fermentasi yang paling baik.Kata kunci : Ekstrak Allium sativum, mineral mikro organik, populasi mikroba,  fermentasi rumen,  sapi potongCH Prayitno and N Hidyat/Animal Production 15(1):69-75, January 2013 
Effect of Supplementation of Garlic Husk Extract to The Feed of Dairy Goats on The Fermentation Product and Ruminal Microbe Subagyo, Yusuf; Prayitno, Caribu Hadi
ANIMAL PRODUCTION Vol 16, No 1 (2014): January
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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Abstract

Abstract. This study was designed to examine the effects of supplementation with husk extract of garlic (Allium sativum) in the feed of dairy goats containing sufficient amount of  organic minerals (Selenium, Chromium and Zinc) on the fermentation and microbes in the rumen. The materials used in this study were the rumen fluid of goat, goat ration composed of 60% grass, 35% tofu, and 5% concentrate (CP 11.90%, CF 28.57%, 60.94% TDN). The research method was experimental using a completely randomized design (CRD). The treatments were tested, namely R0: control diet; R1: R0 + Cr + 1.5 ppm 40 ppm Zn lysinat + 0.3 ppm Se; R2: R1 + 15 ppm  of garlic husk extract (Allium sativum); R3: R1 + 30 ppm garlic husk extracts ; R4: R1 + 45 ppm  of garlic husk extrat; and R5: R1 + 60 ppm  garlic husk extract. The results showed that the treatment effect on a decrease in dry matter (DMD) and  organic matter digestibility (OMD), protozoa and total gas in total, however, there was an increase in total VFA concentrations. The treatment gave a linear response to the DMD, ie Y = 50.412 - 0.1651X  and OMD,  Y = -0.1768X + 50.319. However, in response VFA is cubic, with a line equation Y = 203.16 - 3.2646X + 0.2447X2- 0.0033X3. It culd be concluded that  supplementation of garlic husk extract and organic minerals can improve rumen fermentation with the best level at 25 ppm.Key words:  Garlic husk extract, micro minerals, rumen fermentation, dairy goat Abstrak.  Penelitian ini dirancang untuk mengkaji pengaruh suplementasi ekstrak kulit bawang putih (Allium sativum) dalam pakan kambing perah yang tercukupi mineral organik (Selenium, Chromium dan Seng) terhadap hasil fermentasi dan mikroba  pada rumen. Materi yang digunakan adalah  cairan rumen kambing, ransum kambing yang tersusun atas 60% rumput gajah, 35% ampas tahu dan 5% konsentrat (PK 11,90%, SK 28,57%, TDN 60,94%). Metode penelitian adalah eksperimental menggunakan Rancangan Acak Lengkap (RAL).  Perlakuan yang diujicobakan yaitu R0: pakan kontrol; R1: R0 + 1,5 ppm Cr + 40 ppm Zn lysinat + 0,3 ppm Se; R2: R1 + 15 ppm ekstrak kulit bawang putih (Allium sativum); R3: R1 + 30 ppm ekstrak kulit bawang putih ; R4: R1 + 45 ppm ekstrak kulit bawang putih ; dan R5: R1 + 60 ppm ekstrak kulit bawang putih . Hasil penelitian menunjukkan bahwa perlakuan berpengaruh terhadap penurunan kecernaan bahan kering (KBK) dan kecernaan bahan organik (KBO), protozoa dan gas total, namun demikian terjadi peningkatan konsentrasi VFA total. Perlakuan memberikan respon linier  terhadap  Kecernaan Bahan Kering, yaitu Y = 50.412 - 0,1651X  dan  KBO, Y = -0,1768X + 50,319. Namun demikian pada  VFA   responnya adalah kubik, dengan persamaan garis Y = 203,16 – 3,2646X + 0,2447X2 – 0,0033X3.  Dapat disimpulkan bahwa suplementasi ekstrak kulit bawang putih dan mineral organik dapat memperbaiki fermentasi rumen dengan level terbaik 25 ppm. Kata kunci : ekstrak kulit bawang putih, mineral mikro, fermentasi rumen 
Supplementation of Red Betel Leaf (Piper crocatum) in Dairy Cattle Feed on Fermentation Characteristics by in Vitro Prayitno, Caribu Hadi; Suwarno, Suwarno; Sarwanto, Doso; Hidayatun, Dinar; Solihah, Ma'ratul
ANIMAL PRODUCTION Vol 18, No 2 (2016): May
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (670.658 KB) | DOI: 10.20884/1.anprod.2016.18.2.527

Abstract

The aim of this study was to assess the impact and efficiency of red betel leaf?s extract supplementation in the diet of dairy cattle on fermentation characteristics by in vitro.  The research method was experiment by using completely randomized design.  The treatments that were tested were R1: basal feed, R2:  R1 + 15 ppm of  red betel  leaf (Piper crocatum) extract, R3: R1 + 30 ppm of  red betel leaf (Piper crocatum) extract, R4: R1 + 45 ppm of red betel leaf (Piper crocatum) extract, R5: R1 + 60 ppm of red betel leaf (Piper crocatum) extract. The parameters measured in this study were (1)Dry MatterDigestibility (DMD),(2)Organic Matter Digestibility (OMD)  (3) total gas production  (4) methane production (CH4) and (5)  total Volatille Fatty Acid (VFA).  The data were analyzed using analysis of variance followed Orthogonal Polynomial Test.The results showed that the suplementation red batel extract in the diet of dairy cow was significant (P < 0.01) on DMD, OMD, total gas production, methane production (CH4)  and total VFA.Orthogonal Polynomial test showed the effect of treatment on Dry MatterDigestibility (DMD), total gas and CH4 gas production were in the form of cubic curve, as well as Organic Matter Digestibility (OMD) and Volatille Fatty Acid (VFA) in the form of quadrate curvewith supplementation of red betel leaf.  
Rumination Time and Frequency of Goat Supplemented with Garlic Powder and Organic Chromium Munasik, Munasik; Bahrun, Bahrun; Sigar, Ihsan Yosinanda; Setyaningrum, Agustinah; Prayitno, Caribu Hadi
ANIMAL PRODUCTION Vol 21, No 2 (2019)
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (375.928 KB) | DOI: 10.20884/1.jap.2019.21.2.763

Abstract

This research investigated the effect of supplementing garlic powder and organic micromineral Cr in feed on rumination time and frequency in Ettawah goat (PE). The research was conducted in Gunung Tugel Farm in Patikraja and the Laboratory of Feed Technology in Faculty of Animal Science, Universitas Jenderal Soedirman, Purwokerto, Central Java. This study used 18 male PE aged 1-1.5 years and weighed 18.62 – 22.69 kg. The feed consisted of 60% concentrate and 40% forage (field grass). Other materials included drinking water, 250 ppm garlic powder (Allium sativum), 1.5 ppm organic chromium mineral and eight CCTV camera. The observed parameters were rumination time and frequency. An in vivo experiment in a Completely Randomised Design (CRD) administered three treatments and six replicates, namely R0: control (basal feed); R1: basal feed + 250 ppm garlic powder; and R2: basal feed + 250 ppm garlic powder + 1.5 ppm organic Cr. The result demonstrated a significantly different effect of garlic powder and organic Cr micromineral supplement on rumination time across treatments, i.e. 404 ± 19.6; 382 ± 19.04 and 351 ± 13.87 min/day, respectively, or 379 min/day on average. Rumination frequency was not significantly different across R0, R1, and R2 namely 360 ± 24.03; 359 ± 30.13 and 342 ± 21.10 times/day, respectively. Conclusively, garlic powder and organic Cr micromineral significantly affected rumination time, but not significantly affected rumination frequency of PE goat.
Co-Authors Adi Nugroho Afriska, Helmy Candra Agustinah Setyaningrum, Agustinah Aji, Bayu Purnomo Andri Kusmayadi Anton Budi Prasetyo Bahrun Bahrun Budi Rustomo Daka, Denia Itarioval Deni Setiadi, Deni Doso Sarwanto Dudung Dudung Efka Aris Rimbawanto Hidayatun, Dinar Imbang Haryoko Jalalludin, Jalalludin Kasihandari, Yut Afiffah Muhamad Bata Munasik Munasik N Hidayat, N Nauli Silma Sarifah Novia Rahayu Nugrahani, Triya Farida Nur Hidayat Pambudi Yuwono Perdana Abdi Negara, Pria Pratama, Mohammad Rafi Ruli Yuhana Ryan Pratama Safitri, Alifia Sigar, Ihsan Yosinanda Siti Masruroh Solihah, Ma'ratul SRI RAHAYU Suwarno Suwarno Titin Widiyastuti Tri Rahardjo Sutardi Wardhana Suryapratama Wijaksono, Aqdaam Yusuf Subagyo