Article Per Year (5 Year)
p-Index From 2021 - 2026
0
P-Index
This Author published in this journals
All Journal HAYATI Journal of Biosciences Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Jurnal Ilmiah Aplikasi Isotop Radiasi Indonesian Journal of Agricultural Science Jurnal Hortikultura Jurnal AgroBiogen Jurnal Penelitian Tanaman Industri Buletin Iptek Tanaman Pangan AGRIVITA, Journal of Agricultural Science BERITA BIOLOGI Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Penelitian Pertanian Tanaman Pangan Biosaintifika: Journal of Biology & Biology Education Jurnal Penelitian Tanaman Industri (Littri)
RAGAPADMI PURNAMANINGSIH
BBPPBSGP
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology

Published : 41 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 3 Documents
Search
Journal : HAYATI Journal of Biosciences

 1 
Induction of genetic variant of Artemisia annua L. was conducted through the application of gamma ray irradiation in 2007-2008. The aim was to obtain a plant with high artemisine content > 0.5% and late flowering period of about > 7 month after planting. Tweleve selected genotypes were subsequently examined to gain genetic stability on altitude of 1500, 950, and 540 m asl. The results showed that the plants had shorter flowering age in Cicurug (540 m asl) than that of  in Pacet (950 m asl) and G ENDANG GATI LESTARI ENDANG GATI LESTARI ENDANG GATI LESTARI; MUHAMAD SYUKUR; RAGAPADMI PURNAMANINGSIH; ROSSA YUNITA; ROHIM FIRDAUS
HAYATI Journal of Biosciences Vol. 18 No. 1 (2011): March 2011
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.18.1.16

Abstract

Induction of genetic variant of Artemisia annua L. was conducted through the application of gamma ray irradiation in 2007-2008. The aim was to obtain a plant with high artemisine content > 0.5% and late flowering period of about > 7 month after planting. Tweleve selected genotypes were subsequently examined to gain genetic stability on altitude of 1500, 950, and 540 m asl. The results showed that the plants had shorter flowering age in Cicurug (540 m asl) than that of  in Pacet (950 m asl) and Gunung Putri (1540 m asl). Genotype 8 had the latest age of flowering in the three locations than the other genotypes, however, the growth and biomass were the lowest. Vegetative growth of Artemisia in Pacet and Gunung Putri was better than those in Cicurug. Genotype of 15 in Cicurug and 5A genotype in Gunung Putri and Pacet had higher wet and dry weight than that of two other associates. Based on plant biomass, 5 genotypes from Gunung Putri and Pacet i.e. 1D, 3, 5A, 14, and 15 genotypes were selected, as well as 5 genotypes i.e. 1D, 3, 4, 5A, and 15 genotypes from Cicurug. Analisys on artemisin content successfully obtained 5 selected somaclone lines i.e. 1B, 2, 4, 14, and 3 somaclones.
Genetic Variation of the First Generation of Rodent Tuber (Typhonium flagelliforme Lodd.) Mutants Based on RAPD Molecular Markers NESTI FRONIKA SIANIPAR; DANNY LAURENT; RAGAPADMI PURNAMANINGSIH; IRENG DARWATI
HAYATI Journal of Biosciences Vol. 22 No. 2 (2015): April 2015
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1930.619 KB) | DOI: 10.4308/hjb.22.2.98

Abstract

Rodent tuber (Typhonium flagelliforme Lodd.) is a herbal plant from the Araceae family. The plant has high medical potential and is effective to cure cancer. However, the low level of its genetic variation limits its exploration for desirable traits. The low level of genetic variation in Rodent tuber is mainly due to its asexual reproduction system. It usually reproduces vegetatively via tuber separation. Therefore, gamma irradiation had been applied to rodent tuber in vitro to increase its genetic diversity. The objective of this study was to analyze the genetic diversity of the first generation (MV1) of gamma irradiated rodent tuber mutant using random amplified polymorphic DNA (RAPD) markers. A total of 14 mutant DNA samples were analyzed with 14 RAPD primers. The result showed that 67 out of 123 DNA bands were polymorphic among mutant lines. Based on cluster analysis these mutants showed 0.78-0.97 genetic similarity. Cutting of dendogram at genetic distance of 0.89 produced four main clusters. Mutants with high genetic variation are now available. This increases the opportunity of obtaining mutant lines with high anti-cancer activity.
Increasing Al-Tolerance of Sugarcane Using Ethyl Methane Sulphonate and In Vitro Selection in the Low pH Media Ragapadmi Purnamaningsih; Sri Hutami
HAYATI Journal of Biosciences Vol. 23 No. 1 (2016): January 2016
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (799.665 KB) | DOI: 10.4308/hjb.23.1.1

Abstract

Increased production of sugarcane in Indonesia can be done with extensification sugarcane plantations which largely dominated by acidic upland red-yellow podzolic soil. High aluminium (Al) content and low pH of the soil can inhibit plant growth and development. Tolerant sugarcane in acid soil is the most efficient way, but the adaptive variety is still limited. In vitro culture technique can increase genetic variability to assemble new varieties through somaclonal variation combined with mutation using ethyl methane sulphonate (EMS). The new characters was directed by in vitro selection using AlCl3.6H2O with pH = 4 as a component of selection for resistance to high aluminium. VMC 7616 and PS 862 varieties were used as materials. Mutation induced using EMS at concentrations of 0.1%, 0.3%, and 0.5% for 30, 60 and 120 minutes. Plantlets mutant obtained through callus formation, immersion callus in EMS, in vitro selection, and regeneration of callus. Result of study showed that the long immersion in the EMS solution caused greater damage to the cells, as indicated by the change in callus colour. Callus immersion time in EMS gave greater influence to regeneration compared to concentration of EMS. PS 862 had higher Al tolerance than VMC 7616. Rooting of shoot induced using indole-3-butyric acid (IBA) 3 mg/L.
Co-Authors A G Wattimena Agus Purwito Ashrina, Misky Azrai, Muh. DANNY LAURENT Darliah Darliah Deden Sukmadjaja Diantina, Surya Didy Soepandi Didy Soepandi, Didy Didy Sopandie E.G. Lestari E.G. Lestari E.G. Lestari Endang G Lestari Endang G Lestari Endang Gati Lestari Endang Gati Lestari ENDANG GATI LESTARI ENDANG GATI LESTARI ENDANG GATI LESTARI Enny Sudarmonowati Enny Sudarmonowati GA Wattimena Hutami, Sri I Mariska I Roostika I. Darwati I. Darwati I. DARWATI I. Mariska I. Roostika I. Roostika I. ROOSTIKA Ika Mariska Ika Mariska Ika Mariska Ika Mariska Ika Mariska Ika Roostika Ika Roostika Ika Rostika Ika Rostika Ireng Darwati IRENG DARWATI Laela Sari laela Sari, laela LESTARI, ENDANG GATI Lestari, Endang Gati Lizawati . Mariska, I MARISKA, IKA Mariska, Ika Muhamad Syukur Muhammad Syukur N Khumaida N Khumaida Nesti Fronika Sianipar NESTI FRONIKA SIANIPAR NESTI FRONIKA SIANIPAR Noviati, Arief V. Nur, Amin Nurhayani, Siti Pardal, S.J. Pardal, Saptowo Jumali RITA MEGIA Rita Megia Rohim Firdaus ROHIM FIRDAUS ROHIM FIRDAUS Roostika, Ika Rosa Yunita Rosa Yunita Rosa Yunita Rosaria Rosiana Rosaria Rosiana, Rosaria Rossa Yunita ROSSA YUNITA S.J. Pardal Sari, Laela Siti Nurhayani Slamet . Slamet Slamet Slamet, nFN Soeranto, Soeranto Sri Hutami Sri Hutami Sri Hutami Subagio, Herman Sudarmonowati, Enny Sustiprajitno, Sustiprajitno Syarifah Iis Aisyah Tri P. Priyatno Trias Novita Trikoesoemaningtyas UTAMI, SRI Wahyu Handayati Wahyu Handayati Y Supriati Y Supriati Yati Supriati, Yati Yunita, Rossa