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All Journal Pharmaciana: Jurnal Kefarmasian Jurnal NERS JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA Warta Pengabdian PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat Jurnal Abdi: Media Pengabdian Kepada Masyarakat Jurnal Layanan Masyarakat (Journal of Public Service) Berkala Ilmiah Kimia Farmasi Berkala Penelitian Hayati

Asri Darmawati

Departemen Kimia Farmasi, Fakultas Farmasi, Universitas Airlangga, Surabaya

Author-ID : 3511458

Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health Social Sciences Other

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Antioxidant and inhibitory activity of Roselle Extract (Hibiscus sabdariffa L.) against Methicillin-Resistant Staphylococcus aureus (MRSA) Nuril Fikriyah; Isnaeni Isnaeni; Asri Darmawati
Berkala Ilmiah Kimia Farmasi Vol. 8 No. 1 (2021): June
Publisher : Universitas Airlangga

Background: Hibiscus sabdariffa known as roselle, which belongs to the family Malvaceae, grows in sub-tropical and tropical region including Indonesia. Several studies have reported the benefits of roselle for health. Roselle extract has been shown to have antibacterial effect as a support for antibacterial therapy, especially for case of antibiotic resistance, and antioxidant effect which can neutralize free radicals. Purpose: The aim of this study was obtaining the minimum inhibitory concentration (MIC) and inhibition concentration (IC50) of roselle extract. Methods: Antibacterial effect of roselle extract against Methicillin-Resistant Staphylococcus aureus (MRSA) was tested in nutrient agar media using diffusion method. Antioxidant activity of roselle extract was performed by DPPH. The violet color of DPPH solutions that was reduced by roselle extract were measured using visible spectrophotometer at the wavelength of 516 nm. Result: The result of this study obtained the (MIC) and IC50 of roselle extract were 2,5% and 1251±202,32 ppm, respectively. Conclusion: It can be concluded that roselle extract have antibacterial effect against MRSA and antioxidant effect.

Qualitative Analysis of Rhodamine B in Shrimp Paste at Sumbawa Besar City Markets Using Thin Layer Chromatography Abby Rahmat Kamaruzzaman; Asri Darmawati; Djoko Agus Purwanto
Berkala Ilmiah Kimia Farmasi Vol. 8 No. 2 (2021): December
Publisher : Universitas Airlangga

Terasi biasa diberi pewarna merah agar lebih menarik. Tahun 2013, pernah ditemukan Rhodamin B dalam produk terasi yang beredar di pasar kabupaten Sumbawa Barat. Rhodamin B adalah pewarna merah yang dilarang digunakan untuk makanan. Diantara berbagai produk terasi yang dijual di Sumbawa, terasi khas asli Sumbawa yaitu terasi Empang, perlu dijaga keamanannya agar dapat lebih dipromosikan. Tujuan penelitian ini adalah membuktikan bahwa terasi yang dijual pasar kota Sumbawa Besar tidak mengandung Rhodamin B. Metode yang digunakan untuk identifikasi Rhodamin B adalah kromatografi lapis tipis (KLT). Penelitian ini menggunakan silika gel 60 F254 sebagai fase diam dan fase gerak terpilih yaitu N-butanol:etil asetat:amonia 25% (10:4:5). Sampel adalah produk terasi yang dijual di Pasar Kota Sumbawa Besar, periode sampling bulan Februari dan Maret 2021. Preparasi sampel terasi dengan cara diekstraksi menggunakan etanol dan volume ekstrak yang ditotolkan pada lempeng KLT adalah 2 μl. Parameter validasi metode yang diuji adalah spesifisitas/selektifitas dan batas deteksi (LOD). Hasil penelitian menunjukkan metode ini spesifik untuk Rhodamine B yaitu Rf 0,69, profil spektra yang sama antara baku dan sampel yang diadisi Rhodamin B. Resolusi (Rs) antara noda Rhodamin B dengan noda terdekat lain dalam sampel adalah >1,5. Panjang gelombang serapan maksimum Rhodamin B adalah 544 nm. Nilai LOD sebesar 4,14 ng. Hasil identifikasi terhadap 10 sampel yang terdiri dari 3 sampel terdaftar di BPOM dan 7 sampel tidak terdaftar di BPOM tidak terdeteksi adanya Rhodamin B. Kesimpulan dari penelitian ini adalah semua sampel terasi yang dijual di pasar kota Sumbawa Besar tidak terdeteksi mengandung Rhodamin B.

Validation of UV Spectrophotometry Method for Determination of Lopinavir and Ritonavir Simultaneously Siti Umi Anisah; Asri Darmawati; Amirudin Prawita
Berkala Ilmiah Kimia Farmasi Vol. 8 No. 2 (2021): December
Publisher : Universitas Airlangga

Lopinavir and ritonavir are anti-viral compounds that have similar chemical structures and overlapping UV spectral profiles. The combination of these two compounds is being promoted as an anti-COVID19 drug. Determination of these two compounds simultaneously using UV spectrophotometry method requires special technique so that the result will be valid. The purpose of this study was to obtain a suitable analytical technique using UV spectrophotometry for the determination of lopinavir-ritonavir simultaneously that fulfill the method validation requirement. In this study, the simultaneous equation technique, absorptivity comparison factor, and first derivative technique were used to overcome the effect of lopinavir/ritonavir absorbance at selected wavelengths for determination of each compound simultaneously. The one-way ANOVA statistical test was used to compare the result of the three analytical techniques. The results showed that the three techniques fullfilled the AOAC requirements for selectivity and linearity. The accuracy and precision test result have not met the requirements of the AOAC method validation. Statistically. the one-way ANOVA analysis showed there was a significant difference between the mean recovery of lopinavir using the absorptivity factor and first derivative technique. Whereas, there was no significant differences among the mean of ritonavir recoveries that were determined using those three techniques. As conclusion, that the UV spectrophotometric method using the simultaneous equation technique, the absorptivity factor technique, and the derivative technique for assaying the lopinavir and ritonavir simultaneously met the requirements for selectivity and linearity parameters. However, the accuracy and precision have not met the requirements. The first derivative technique is suitable for further developed for ritonavir and lopinavir determination simultaneously

The Effect of Temperature and Heating Time of Roselle Extract (Hibiscus Sabdariffa L) Powder Solution on Growth Inhibition of Staphylococcus aureus ATCC 25923 Hana Sofiana Maghfira; Isnaeni Isnaeni; Asri Darmawati
Berkala Ilmiah Kimia Farmasi Vol. 8 No. 2 (2021): December
Publisher : Universitas Airlangga

The main ingredients of roselle (Hibiscus sabdariffa L.) which have antioxidant and antibacterial effect were anthocyanins, and flavonoids. Flavonoid compounds were unstable at high temperatures. Meanwhile, the processing of roselle extract powder into ready-to drink or food preparations generally requires the thermal processes. The aims of this study were to determine the effect of temperature and heating time of roselle extract powder solution on the inhibition of microbial growth of Staphylococcus aureus ATCC 25923. The method of this study uses a ready-to- process roselle powder extract sample. The roselle extract powder solution in a screw cap tube was heated at a temperature of 40°C, 50°C, 60°C, 70°C, 80°C, and 90°C on a waterbath, with heating times at each temperature were 15 minutes and 30 minutes. Anti bacterial activity of the sample solution was measured based on the ability to inhibit the growth of Staphylococcus aureus ATCC 25923 by the agar-well diffusion method. The inhibition zone of the sample was measured by caliper. The result of this study showed that inhibition zones caused by the sample that had been heated at those temperature for 15 minutes were 9.55±0.70 mm, 9.53 ±0.04 mm, 9.70±0.14 mm, 9.68±0.24 mm, 10.10±0.14 mm, and 10.25±0.21 mm. Meanwhile, after heating for 30 minutes the inhibition zones were 10.08±0.25 mm, 10.20±0.28 mm, 10.43±0.18 mm, 10.08±0.18 mm, 10.78±0.04 mm, 9.70±0.14 mm. Two ways statistical test ANOVA Randomized Control Block Design with 95% confidence level was used to prove that there was a significant difference berween the mean inhibition zones of each sample. The conclution of this research was, the temperature in the range of 40 -90 oC has no effect on the anti-bacterial activity of the sample. However, the heating time of the sample affected the antibacterial activity of the roselle extract powder solution against Staphylococcus aureus ATCC 25923.

Antimicrobial activity of Streptomyces spp. isolates from vegetable plantation soil Isnaeni Isnaeni; Idha Kusumawati; Mega Ferdina Suwito; Asri Darmawati; Ni Made Mertaniasih
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 21 No 2 (2016): June 2016
Publisher : The East Java Biological Society

Fifteen Streptomyces isolates were isolated from soil in some different location on vegetable plantation at agriculture standard condition. The isolates were assessed for their antibacterial activity against Mycobacterium tuberculosis (MTB) ATCC H37RV and mycobacterial which isolated from Dr. Soetomo Hospital patients in Surabaya. The Interna-tional Streptomyces Project 4 (ISP4) and Middlebrook 7H9 (MB7H9) wwere used as growth or fermentation medium. The screening of inhibition activity was performed using turbidimetry and spot-test on agar medium. Results shown that 33.3% of the isolates (5 isolates) have anti-mycobacterial activities. The first line anti tuberculosis drug rifampicin, (RIF), ethambutol (EMB), isoniazid (INH), and pyrazinamide (PZA) were used as standards or positive controls with concentration 20 ppm. Optical density of crude fermentation broth concentrated from five isolates relatively lower than five anti-tuberculosis drug activity standard, although their activities against some microbial were similar to the stand-ard at spot-test. The most efficient isolate shown anti-mycobacterial activity was Streptomyces B10 which identified as Streptomyces violaceousniger. In addition, fatty acid methyl ester (FAME) profile of gas chromatography-mass spec-trometry chromatogram of each isolates were studied and compared to Streptomyces spp.

The Improvement of Food Additives and Good Food Production Method Knowledge on Sumba Island Noor Erma Nasution Sugijanto; Juni Ekowati; Heinrich Dengi; Asri Darmawati; Dewi Isadiartuti; Catur Dian Setiawan; Diajeng Putri Paramita; Melanny Ika Sulistyowati; Putri Hamidah Khairunnisa; Imamatin Nufus Melania; Muhammad Ilham Royyan Nafi; Sugijanto Kartosentono
PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat Vol 7 No 2 (2022): PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/pengabdianmu.v7i2.2783

In order to ensure good health and well-being in the community during the pandemic, especially in the food business in Sumba, several problems for MSME partners have been identified, namely related to food production, consumption, and misunderstanding of regulations in the food and beverage business which can be a problem for the health community. The solutions offered to overcome these problems are training in webinars and talk shows on Food Business Production Orders; Knowledge of Food Additives; Good Food Production Methods; Household Food Industry Product Licensing (CPPB-IRT); and Simple Marketing for the Food Business. This community service aims to improve MSMEs' quality and business capacity on Sumba Island, which is increasing and has become a model for similar MSME communities because they have not received similar guidance from any agency. The pre and post-test analysis results showed an increase in knowledge, especially on aspects of the types of food that were not allowed to receive SPP-IRT, food coloring, permitted food additives, how to get Halal certification, and spot tests to find out hazardous materials. The implementation evaluation showed that more than 58% stated that the material was acceptable. More than 99% stated that the registration was carried out well. For delivering materials and discussions, 99% said they were delighted. This webinar activity concludes that the material topics have been as needed, there is an increase in partner knowledge, and the implementation has been carried out well, based on pre-post test evaluation data and evaluation of the implementation of activities agency.

PELATIHAN CARA PRODUKSI DAN REGISTRASI MAKANAN YANG BAIK, AMAN DAN HALAL BAGI MASYARAKAT KHALAYAK SMK NEGERI 4 BOJONEGORO isnaeni isnaeni isnaeni; Suko Hardjono Suko Hardjono Suko Hardjono; Asri Darmawati Asri Darmawati Asri Darmawati; Noor Erma Nasution Noor Erma Nasution Sugijanto; Juni Ekowati Juni Ekowati Juni Ekowati; Sugijanto Sugijanto Randiman; Muhammad Yuwono Muhammad Yuwono Muhammad yuwono
Jurnal ABDI: Media Pengabdian Kepada Masyarakat Vol. 6 No. 1 (2020)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/ja.v6n1.p6-12

Community Service Activities (PENMAS) were held in August 2018 in Kapas, Bojonegoro with a target audience of SMKN 4 communities, totaling 107 people, consisting of students majoring in Cooking, members of Dharma Wanita, Chefs from 2hotels, several UMKM Food and Beverages around the school, teachers and partisipants from other schools. The activity was also attended by the Principal, the Head of the Education Office who witnessed the signing of the MOU between the PENMAS Team and the school. One of the Departments managed by SMK 4 is Tata Boga with thelearning outcome to prepare entrepreneurs or businessmen of food products. As a vocational school that has held the ISO 9001 2008 certificate, the school is obliged to prepare graduates according to the demands of their competence. Based on preliminary studies, information was obtained that there were several problems related to food consumption and production and government regulationin the case of traded food businesses. The training materials provided in this training were: Food business production regulation (1), How to make good food (2),Ingredients additional allowable food (3), Prohibited food additives and substitute alternatives (4), Good packaging methods and techniques for food and beverage (5), Halal Assurance System (SJH) and Halal food product Certification Process (6) and Simple marketing for food business (7). Based on the evaluation of the pre-test and post-test resulted that after the training participants' understanding showed an increasing trend. Three topics that showed a significant increase in understanding were: Requirements for clean and healthy food (1), Introduction of food coloring logos (2), and Examples of halal food ingredients (3). PENMAS activities will be followed up with intensive coaching activities for students of SMKN4 which are included in the group of beginner beverage food businesses.

Identification of Paracetamol and Caffeine in Jamu Powders Simultaneously Using TLC-Densitometry Wahyuningsih, Etik; Isnaeni, Isnaeni; Asri Darmawati; Annisa Kartika Sari
Berkala Ilmiah Kimia Farmasi Vol. 10 No. 1 (2023): June
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/bikfar.v10i1.44242

Paracetamol and caffeine were chemical compounds suspected to be illegally added to traditional herbs claimed as rheumatics drugs. Identification of paracetamol and caffeine was conducted on five samples of jamu powder obtained from the Depot Jamu in Surabaya. This study aimed to simultaneously identify paracetamol and caffeine commonly found in traditional medicine, one of which was jamu powder, using thin-layer chromatography densitometry (TLC-Densitometry). Evaluation of the presence of paracetamol and caffeine in the product of jamu was performed by thin-layer chromatography with silica gel GF254 and chloroform-ethyl acetate (1:1) as the stationary and mobile phases, respectively. The spots on the TLC plate were detected using a UV light at 254 nm, and the areas were measured by a Camag TLC scanner. The TLC profile demonstrated good separation of paracetamol, caffeine, and other substances contained in the products. The retardation factors (Rf) of paracetamol and caffeine were 0,42 and 0,26, with detection limits of 0,0125 µg/spot and 0,05 µg/spot, respectively. The simultaneous identification of caffeine and paracetamol using thin-layer chromatography densitometry revealed that none of the five samples were detected to contain paracetamol and caffeine. Keywords: Simultaneously, Identification, Paracetamol, Caffeine, Jamu Powder, TLC- Densitometry

Co-Authors Abby Rahmat Kamaruzzaman Achmad Toto Poernomo Amirudin Prawita Annisa Kartika Sari Aprelita Nurelli Dwiana Ariya Wijaya Putra Dewi Isadiartuti Diyah, Nuzul Wahyuning Djoko Agus Purwanto Eka Aprillia Suhartini Etik Wahyuningsih Febri Annuryanti Hana Sofiana Maghfira Heinrich Dengi Idha Kusumawati Imamatin Nufus Melania Isnaeni Isnaeni Isnaeni isnaeni, isnaeni isnaeni Juni Ekowati Juniar Mochtar Juniar Moechtar Mega Ferdina Suwito Melanny Ika Sulistyowati Mochammad Yuwono Muhammad Ilham Royyan Nafi Muhammad Mu’amar Fathoni Muhammad Yuwono Muhammad Yuwono Muhammad yuwono Muhammad yuwono, Muhammad Yuwono Muhammad Yuwono Ni Made Mertaniasih Noor Erma Nasution Noor Erma Nasution Sugijanto Noor Erma Nasution Sugijanto Nuril Fikriyah Paramita, Diajeng Putri Purwaningsih - Putri Hamidah Khairunnisa Randiman, Sugijanto Sugijanto Rara Dyah Chrissanti Riesta Primaharinastiti SETIAWAN, Catur Dian Siti Umi Anisah Sudjarwo Sudjarwo Sugijanto Kartosentono Sugijanto Sugijanto Randiman Sugijanto, Noor Erma Nasution Noor Erma Nasution Suko Hardjono Susanti Susanti

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