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All Journal Jurnal Pengendalian Hayati Agrovigor: Jurnal Agroekoteknologi Plumula : Berkala Ilmiah Agroteknologi Agrifor : Jurnal Ilmu Pertanian dan Kehutanan Jurnal Ilmu-Ilmu Pertanian Indonesia Jurnal Agroekoteknologi Jurnal Perlindungan Tanaman Indonesia JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Jurnal Ilmiah Universitas Batanghari Jambi BIOEDUSCIENCE Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) JPT : JURNAL PROTEKSI TANAMAN (JOURNAL OF PLANT PROTECTION) JURNAL AGROHITA Jurnal Pembelajaran dan Biologi Nukleus Jurnal Agrotropika PLAKAT : Jurnal Pelayanan Kepada Masyarakat Nusantara Science and Technology Proceedings Berkala Penelitian Hayati Jurnal Pengabdian Kepada Masyarakat JPMP Agrocentrum Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Bioteknologi & Biosains Indonesia
Yenny WURYANDARI
Program Studi Agroteknologi, Fakultas Pertanian, UPN “Veteran” Jawa Timur
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mechanical Engineering Medicine & Pharmacology Social Sciences Other

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Journal : Nusantara Science and Technology Proceedings

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Population Dynamics of Pseudomonad fluorescent Isolate Pf-122 In Formula in Different Save Containers Wuryandari, Yenny; Wiyatiningsih, Sri; Suwandi
Nusantara Science and Technology Proceedings 5th International Seminar of Research Month 2020
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2021.0933

Abstract

The development of biopesticide formulas is carried out by optimizing the use of natural media materials. The formula is made easily, cheaply, and can still maintain the viability of bacteria. This study aims to determine the effect of various formulas and storage containers on the viability of Pseudomonad fluorescent Pf-122. Eight kinds of formulas include; 1) Non-hydrolyzed rice crab at a dose of 400 (K400), 2) at a dose of 200 (K200), 3) Hydrolyzed papaya rice crab at a dose of 400 (KP400), 4) at a dose of 200 (KP200), 5) Pineapple hydrolyzed rice crab with a dose of 400 (KN400), 6). at a dose of 200 (KN200), 7) Media King'S B at a dose of 400 (KB400), 8). at a dose of 200 (KB200). The formula is stored in a plastic and paper storage container. Population dynamics were observed at 1-week intervals. In the paper container, at week 1, the highest bacterial population was in the KP 400 and K400 formulas. At weeks 3 to 8, the population experienced a very rapid decline in all formulas, except for the KN400 formula. In the KN400 formula, at weeks 2 and 3, the population reached 107 CFU / ml and experienced a slow decrease until week 8 (population 101CFU / ml). In plastic containers, at week 1, the highest bacterial population was in the formula KB200, K200, and KP400. At the 3rd to 8th week of observation, the population experienced a rapid decline in all formulas, except for formula K400. In the K400 formula, starting from weeks 2 and 3, the population reached 106 CFU / ml and decreased until the 8th week (population 103CFU / ml). As for the KN400 formula, it can still last until the 7th week with a population of 102 CFU / ml. The longest survival rate of Pf-122 bacteria in the two storage containers was the KN400 formula. The best survival time of Pf-122 bacteria (long-lasting time and high population) is the formula of rice crab with a dose of 400 (K400) in a plastic container.
The Influence of Some Types of Biological Agent Formula and the Different Application Time on the Population of the Biological Agent Bacteria and The Chili Plants Yenny Wuryandari; Sri Wiyatiningsih Suwandi
Nusantara Science and Technology Proceedings 4th International Seminar of Research Month
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2019.0446

Abstract

One alternative for suppressing disease and promoting plant growth is to use the formula for biological agents. The research aims to determine the best formulation of Pseudomonad fluorescent Pf-122 and to determine the right application time in stimulating the growth of chili plants. The formula is made by mixing bacterial active ingredients with semi-natural media of rice crab by hydrolyzing using pineapple and papaya, as well as organic fertilizer. The study used 2 treatment factors. The first factor is the type of formula consisting of control using only Pf-122 (F0), Pf-122 + K (rice crab) (F1), Pf-122 + KN (rice crab by hydrolyzing using pineapple) (F2), Pf-122 + KP (rice crab by hydrolyzing using papaya) (F3). The second factor is application time; watering five days before planting and watering while planting. The results showed that plant growth, namely plant height, number of leaves, root length, and root weight did not differ statistically significantly from all treatment types of application. When viewed from the time of application of the formula, there is also no difference in the growth of chili plants. However, when viewed from the average value, the control without formula tends to show a lower growth value compared to that given the formula. In the generative growth phase, which is when the flowers appear, the chili plants are given a formula faster than without the formula. Application for all types of formulas, the time of occurrence of flowers is not significantly different. The number of Pf-122 populations in the rhizosphere decreases and increases are unstable in all formula applications. The high Pf-122 population in the rhizosphere does not show high chili growth either
Inhibitory Test of Bacillus sp. against Phytophthora palmivora Causes Cocoa Fruit Rot Disease Devi Tria Anjarsari; Endang Triwahyu Prasetyawati; Yenny Wuryandari
Nusantara Science and Technology Proceedings Seminar Nasional Agroteknologi Fakultas Pertanian UPN “Veteran” Jawa Timur 2021
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2022.2003

Abstract

Fruit rot is one of the important diseases in cocoa fruit, which is caused by Phytophthora palmivora. Farmers often control these pathogen using chemical pesticides. However, this method has a detrimental impact on the environment and health. More environmentally friendly fruit rot disease control can use biocontrol agency. Bacillus sp. is one of the biocontrol agents that is able to produce antibiotics that can inhibit the growth of pathogens, especially soil-borne pathogens and have the ability to colonize plant roots. The purpose of this study is to determine the potential of Bacillus sp. in inhibiting the growth of the pathogenic fungus Phytophthora palmivora in vitro. This study used two experimental factors, type of isolates Bacillus sp. Ba-6 isolate, Ba-9 isolate, Ba-12 isolate, Ba-15 isolate, and Ba-17 isolate and the concentration of Bacillus sp. (106 cfu/ml and 109 cfu/ml). The parameters observed were the inhibitory power and the mechanism of inhibition of Bacillus sp. against Phytophthora palmivora. The results of the best in vitro inhibition test on Ba12K2 treatment with an inhibition of 61% with an inhibitory mechanism, namely antibiosis, as evidenced by the presence of zones of inhibition and swelling of the hyphae.
Pest and Disease Control in Cavendish Banana Seedlings Resulting from Tissue Culture Indah Sari Dwi Agustin; Penta Suryaminarsih; Putranto Sasikirono; Yenny Wuryandari
Nusantara Science and Technology Proceedings Seminar Nasional Agroteknologi Fakultas Pertanian UPN “Veteran” Jawa Timur 2021
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2022.2013

Abstract

Cavendis banana cultivation can use tissue culture as one of the developments of cultivation in the era of disruption. However, the results of tissue culture are very susceptible to attack by nuisance organisms during acclimatization to plant culture. The control carried out against the attack of plant-disturbing organisms in addition to using fungicides and insecticides, also applies preventive control. The purpose of this study was to determine an effective and smart way to control pests and diseases in tissue cultured Cavendish banana seedlings. Preventive control of pests and diseases using the method of thinning the seeds and soaking the seeds with fungicides. Data analysis was carried out using descriptive and parametric data. This study was conducted using a completely randomized design (CRD) with four control treatments. Each treatment was repeated 4 times. The control treatments carried out consist of: Control (A), preventive control (B), chemical control (C), and a combination of preventive and chemical control (D). The results of the control carried out showed that the combination of preventive and chemical control treatments gave significant results in inhibiting the attack of pests and diseases of Cavendish banana seedlings from tissue culture.
Utilization of Neem Leaves as a Biological Pesticide for the Control of Anthrachnose Diseases in Dragons (Hylocereus sp.) Nolla Dwi Elvira; Yenny Wuryandari
Nusantara Science and Technology Proceedings Seminar Nasional Agroteknologi 2022
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2023.3109

Abstract

Dragon fruit (Hylocereus sp.) has problems in its cultivation related to Plant Pest Organisms (OPT). One of the pathogens that attack dragon fruit plants is Colletotrichum sp. which can cause anthracnose. dragon fruit pest control method can be done by using biological pesticides from neem leaf extract. Pesticides from plant extracts are sources of various compounds that are rich in various types of active ingredients such as secondary metabolites that play an important role in the process of interacting or competing and protecting themselves from competitors. The purpose of this study was to determine the effectiveness of neem leaf pesticide for controlling anthracnose on dragon fruit plants. The method used is testing the inhibitory power of neem leaf biological pesticides against anthracnose on dragon fruit plants in vitro. The concentration treatments of biological pesticides included 0%, 20%, 40%, and 60%. The results obtained showed that the biological pesticide neem leaf with a concentration of 20% already had an inhibitory power against the pathogen Colletotrichum sp. which is quite good because at that concentration it already has a fairly high antifungal compound. The magnitude of the inhibitory power of neem leaf biological pesticides at a concentration of 20% was 53.94%.
Promotion of Beauveria bassiana Mushroom on Different Growing Media and Its Pathogenicity on Insects Lukmanul Hakim; Yenny Wuryandari
Nusantara Science and Technology Proceedings Seminar Nasional Agroteknologi 2022
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2023.3116

Abstract

One type of entomopathogenic fungus that is known to be effective in controlling important plant pests is the Beauveria bassiana fungus. The fungus B. bassiana, has a high reproductive capacity, is easy to produce and under unfavorable conditions can form spores that can last a long time in nature. The purpose of this study was to determine the density and viability of B. bassiana spores on corn and rice media and to investigate the process of B. bassiana fungal infection in the test insects. The method used is; Fungal propagation test on Rice and Corn Media, Microscopic Observation of Fungus from Corn and Rice Media, Calculation of Fungus Spore Density, Calculation of Fungus Viability and Pathogenicity Test. The research results obtained are as follows; Results The propagation of B. bassiana mushroom grew on the seventh day, the fungus growth was faster in rice media than in corn media. The spore density of the fungus B. bassiana clove bondowoso in corn media was 3.75x108 spores/ml, rice media was 4.58x108 spores/ml, B. bassiana banyuwangi coffee medium was 3.5x108 spores/ml, rice media was 3.75x108 spores/ml. The viability of the fungus B. bassiana, clove bondowoso and banyuwangi coffee on rice and corn media was obtained on average above 60%. The results of the pathogenicity test of B. bassiana on insects began to appear on the tenth day after the baiting process.
Carrier Materials Differences on Bioencapsulation Formulation Affect Bacillus sp. Isolates Wuryandari, Yenny; Suryaminarsih, Penta; Lestari, Safira Rizka
Nusantara Science and Technology Proceedings 8th International Seminar of Research Month 2023
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2024.4162

Abstract

Biological agent Bacillus sp. Based on the results of previous research, direct use of isolated microorganisms BCZ14, BCZ16, and BCZ20 was able to suppress Fusarium wilt disease. However, Bacillus sp. formulations in suspension form are less effective when applied in the field. This research focuses on analyzing the viability of three isolates of Bacillus sp. against several combinations of bioencapsulation coating materials. The experimental design used was a completely randomized design with a combination of treatment of three isolates and three types of coating materials, namely sodium alginate (B1), carrageenan (B2), and chitosan (B3) each 2%. Each treatment combination was repeated five times. Based on the experiments that have been carried out, the bioencapsulation formulation using 2% chitosan coating material was able to maintain the viability of the three isolates of Bacillus sp. BCZ14, BCZ16, and BCZ20. The population density of the three isolates of Bacillus sp. persisted consistently from the first week to the last week of observation. Therefore, the 2% chitosan coating material can be said to be able to maintain the viability of Bacillus sp. within six weeks.
Identification of Insects on Soybean Planting at UPT Pengembangan Benih Padi dan Palawija Singosari Yenny Wuryandari; Annisa Zukhruf Oktavia Salamah
Nusantara Science and Technology Proceedings Seminar Nasional Agroteknologi 2023
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2024.4007

Abstract

Corruption obliteration presently focuses on three main issues: prevention, obliteration, and asset recovery. Eradicate corruption not only focuses on preventing and eradicating but also improves the terms of punishing the perpetrators and is also associated with seeking to recover state financial losses from the proceeds of corruption crimes. The provisions in question are of great hope for systematic and comprehensive anti-corruption measures, which feature not only criminal prosecution of corruption crimes but also the protection of national finance, that is, the obligation to return state funds from the perpetrators of criminals. Corruption crimes can be used for national development. Therefore, normatively it would not be excessive if the provisions of Article 18 of Constitution no. 31 of 1999 in conjunction with Constitution no. 20 of 2001 regarding the Obliteration of deceits of corruption can be an effective legal instrument for compensating the State for losses caused by corruption crimes.
Analysis of Encapsulation Efficiency Bacillus sp. Based Variations in Sodium Alginate Concentration in the Beads Mochammad Mirza Saputra; Yenny Wuryandari; Noni Ramadhini
Nusantara Science and Technology Proceedings The 4th International Conference on Agriculture and Environmental Sciences (ICAES) 2023
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2024.3901

Abstract

The use of biological agents such as Bacillus sp. Bacteria have started to be widely used by farmers as a new form of control. Bacillus sp. needs to require a special method in its application because it has certain living conditions, and Bacillus sp. is susceptible to environmental stress like other microorganisms. Apply Bacillus sp. as a biological control agent in the field, it needs to be formed into a formulation to be more effective and efficient when applied. Liquid formulations often used cannot maintain the viability of bacteria in the soil. The bioencapsulation formulation in the form of microcapsules is considered more effective in maintaining the viability of bacteria in the soil. This study aims to determine the effect of concentration on the encapsulation efficiency of Bacillus sp. inside the microcapsule. Beads are made using the extrusion method by combining a sodium alginate suspension of 1%, 1.5%, and 2% concentration and a bacterial suspension and dropping it into a calcium chloride solution. The encapsulation efficiency test was carried out once by extracting the bacteria on the beads, growing them in the growth medium, and then observing them. The results of the encapsulation efficiency test with a concentration of 1%, 1.5%, and 2% had values of 1%, 0.36%, and 1.35%, respectively. The difference in the results of this encapsulation efficiency indicates that there is an effect of sodium alginate concentration on the value of encapsulation efficiency.
Inventory of Fungi on Imported Wheat Grains from Canada at the Large Agricultural Quarantine Center Surabaya Nirwana Septania Galih Perwira Moekti; Yenny Wuryandari
Nusantara Science and Technology Proceedings The 4th International Conference on Agriculture and Environmental Sciences (ICAES) 2023
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2024.3905

Abstract

Wheat is one of the most popular commodities and is often consumed by the Indonesian people. In 2019, wheat was consumed at least 30.5 kg/year by the Indonesian people. The import of wheat in large quantities allows the risk of carrying OPTK, especially fungi with OPTK category A1, so detection and identification of wheat seeds entering Indonesia is needed. This study aims to detect and determine the fungi found in wheat seeds from Canada (Canada I and Canada II) using the Washing Test method at the Surabaya Agricultural Quarantine Center. The Washing test method is a washing method to release fungi on the surface of seeds using sterile distilled water and a centrifuge. The stages of this method are as follows sampling of wheat seeds from Canada, detection using the washing test method, and microscopic identification. The results of detection and identification on wheat seeds did not find the target pest but other fungi were found in Canada I sample, namely Cladosporium variabile, Cladosporium sp., Puccinia sp., Puccinia graminis and in Canada II sample Cladosporium variabile, and Puccinia graminis. Further quarantine measures for imported wheat grains are exemptions with the issuance of a plant exemption certificate (KT-9). Service procedures at the BBKP Surabaya laboratory are carried out in accordance with Law No. 21 of 2019, MOA No. 25 of 2020 and ISO 17025 with 2P quarantine measures (Inspection and Exemption).
Co-Authors Achmad Fiqri Agus Wahyudi Aisyah Lulu Hariyanto Anjani, Ajeng Distya Annisa Zukhruf Oktavia Salamah arfah Arika Purnawati Bambang Hadisutrisno Bambang HADISUTRISNO Budi Rianto Devi Tria Anjarsari Devi Tria Anjarsari Didiek Tranggono Edo Pandu Pratama Erlyana Desy Rahmawati Fadia Nur Rahma Budiarto Frida Nur Aisah Gita Pawana Hera Amalia Putri Belangi Herry Nirwanto HIDAYATI, KIKI I. Hartana Indah Sari Dwi Agustin Indarwati Indarwati Kaswan Badami Kiki Sandra Amelia Kusuma Wardhani Mas'udah Lestari, Safira Rizka Lukmanul Hakim M. Husni Tamrin, M. Husni Maroeto ., Maroeto Maroeto Maroeto Mas’udah, Kusuma Wardhani Mochammad Mirza Saputra Mochammad Mirza Saputra Munir, Ahmad Arsyad Nauval Zakqy Nirwana Septania Galih Perwira Moekti Nolla Dwi Elvira Noni Rahmadhini Noni Ramadhini Nova Andriani Novita Endah Ariani Novita Endah Ariani Nuriatus Sholikhah Penta SURYAMINARSIH Perwira Moekti, Nirwana Septania Galih Prasetyawati, Endang Triwahyu Putranto Sasikirono Putri, Ratih Nadhila Raharja, Wildan Taufik Ratu Yusnita Zhalsabilla Rulinggar, Nia Safira Rizka Lestari Safira Rizka Lestari Sagala, Yosua Nathanael Itona Saputra, Mochammad Mirza Saputra, Mochammad Mirza Setiawan Setiawan Shelomitha Kumala Mawardhany Sherly Arindawati Sri Wiyatiningsih Sulistyanto Sulistyawati, Hanik Suwandi Syamsul Arifin Taufik Hidayat Tr Mujoko Tri MUJOKO Triwidodo Arwiyanto Triwidodo ARWIYANTO Wahyu, Endang Tri Windriyanti, Wiwin Yovita Nathania Yuwono, F. Tsara Nafisah Zainal Abidin Achmad Zakqy, Nauval