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Identification of Sago-Pulp Amylolytic Bacteria and Its Utilization for Granulated Fertilizer Uswatun Hasanah; Tri Ardyati; Dian Siswanto
The Journal of Experimental Life Science Vol. 11 No. 2 (2021)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2021.011.02.02

Previous research stated that bacteria isolated from sago waste from the traditional sago industry in Palopo had the potency to produce amylase. This study aims to confirm the ability of bacteria isolated from sago pulp waste to produce amylase, evaluate the ability of these amylolytic bacteria to produce IAA fix nitrogen, identify selected bacteria, and apply selected bacteria into granules biofertilizers. Bacteria were isolated from sago pulp waste and grown on a 1% starch agar medium. The amylolytic activity was analyzed qualitatively using iodine and quantitatively using the 3,5-Dinitrosalicylic Acid (DNS) method at pH 6. Amylolytic bacteria were analyzed for IAA production using Salkowsky reagent and nitrogen fixation ability through the Serra Kit method. Potential bacteria were identified based on the similarity of the 16S rDNA sequence. The selected bacteria were grown in a 10mL NB medium, and then bacteria were inoculated and air-dried to obtain the granules. The selected bacteria were put into granules and re-isolated using the total plate count (TPC) method. Eight amylolytic bacteria were obtained from sago pulp waste. L1E isolate had the highest amylolytic activity of 1.228 U.mL-1, and L1D isolate had the highest IAA production of 69.8 g.mL-1. Isolate L1E was identified as Alcaligenes faecalis with a 99.45% similarity index, and L1D was identified as Serratia surfactantfaciens with a 99.09% similarity index. Isolate L2G was identified as Alcaligenes aquatilis with a similarity index of 99.8% of the 16S rDNA sequence. The viability of bacteria from granules was 1.41 x 108 CFU.g-1.Keywords: Amylase enzyme, amylolytic bacteria, granulated fertilizer.

Variation of Fruit Color in Cakra Hijau, G1/M8 and HV-149 Chilli Pepper Cultivar: Physiology and Molecular Approach: Variation of Fruit Color in Cakra Hijau, G1/M8 and HV-149 Cultivar Arumingtyas, Estri Laras; Zakiyah, 'Ainun Sayyidah; Monica, Rosina; Siswanto, Dian
Journal of Tropical Life Science Vol. 13 No. 3 (2023)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.13.03.13

The fruit color of chili pepper is an important characteristic in identification and classification and is often used as the basis for determining consumer preferences. Information on the relationship between chili fruit color and its molecular profile is very important in supporting selection activities in plant breeding. This study aims to identify genetic diversity associated with the fruit color of three genotypes of chili (Capsicum frutescens L.): Cakra Hijau, HV-149 and G1/M8, using Random Amplified Polymorphic DNA (RAPD) and Simple Sequence Repeat (SSR). Morphological confirmation was carried out according to Capsicum descriptors. Nineteen RAPD markers and six SSR markers were used for genetic variability assessment. Genetic variation was analyzed using the unweighted pair group method with the arithmetic mean and the Jaccard similarity index. The three chili genotypes had different fruit colors at each maturation stage. The immature Cakra Hijau fruit is dark green and turns dark red as it ripens. The immature fruits of the G1/M8 line are light green and turn red when ripe. Finally, immature HV-149 fruits are dark green and yellow when ripe. The SSR markers used in this study were unable to show polymorphism. On the other hand, the RAPD marker successfully detected genetic variation in the three chili genotypes and resulted in a total of 49 alleles. The average value of polymorphic information content of the RAPD primers used ranged from 0 to 0.296, with the highest index indicated by OPA-1. The dendrogram shows the separation of the three genotypes into two main clusters, with the first cluster consisting of the HV-149 variety and the second cluster consisting of Cakra Hijau and G1/M8 lines. This study revealed that there are genetic variations based on the morphological characteristics of fruit color at each ripening stage and RAPD band profile. The RAPD marker was more effective than the SSR marker for identifying the genetic diversity of fruit color in the three chilies studied.

Penguatan Kapasitas Masyarakat Berbasis Geoinformasi dan Pengembangan Agrowisata Kopi di Kecamatan Kalipuro, Banyuwangi Siswanto, Dian; Wicaksono, Kurniawan Sigit; Sahiruddin, Sahiruddin; Hakim, Luchman; Rahardi, Brian
Jurnal Pengabdian Masyarakat Biologi dan Sains Vol 3, No 1 (2024): Jurnal Pengabdian Masyarakat Biologi dan Sains
Publisher : Universitas Indraprasta PGRI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30998/jpmbio-sains.v3i1.2742

Tujuan pengabdian masyarakat di Kecamatan Kalipuro ini adalah meningkatkan pengetahuan masyarakat tentang keterkaitan tanaman dan lingkungannya, terutama aspek-aspek terkait kondisi geografis menjadi dasar dari penguatan produk kopi, memperbaiki metode pengolahan kopi dengan transfer dan difusi teknologi roasting kopi, memberikan edukasi terhadap sistem pertanian berbasis geoinformasi dan agrowisata melalui IPTEK bagi masyarakat. Metode yang digunakan adalah pelatihan dan Forum Grup Discussion (FGD) untuk mencapai tujuan yang ditetapkan. Pemahaman yang terintegrasi antara penggunaan geoinformasi, teknologi pengolahan kopi, dan pengembangan agrowisata dalam sektor kopi rakyat memiliki potensi untuk meningkatkan pendapatan masyarakat sekaligus menjaga kelestarian kopi menuju indikasi geografis kopi Banyuwangi.

Characterization of Microplastic Degrading Indigenous Bacteria from Ambon Bay Waters Zakaria, Idham Halil; Siswanto, Dian; Mustafa, Irfan
HAYATI Journal of Biosciences Vol. 31 No. 5 (2024): September 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.5.867-879

Microplastic degradation by bacteria can degrade low-density polyethylene (LDPE). This study aimed to analyze the potential of Ambon Bay bacteria for microplastic degradation, the condition of microplastics after degradation, and identification of the potential for microplastic degradation. The results of isolation revealed as many as 20 bacterial isolates, which correlated with physicochemical conditions in the waters of Ambon Bay. Nine of them could degrade microplastics as indicated by the presence of a clear zone, namely KA1, KA2, KA3, KA4, KA5, KA9, KA10, KS6, and KS8. They were checked for biofilm formation, microplastic hydrophobicity, and percentage of microplastic weight reduction. Four isolates with the highest percentage of microplastic weight reduction on day 40 were KA1, KA2, KA3, and KA10 at 36.19%, 10.16%, 28.39%, and 17.07%, respectively. The results of LDPE microplastic degradation showed differences using field emission scanning electron microscopy-energy dispersive spectroscopy (FESEM/EDS), attenuated total reflection-fourier transform infrared (ATR-FTIR), and X-ray diffraction (XRD). The bacterial isolates identified were KA1 (Bacillus cereus), KA2 (Bacillus toyonesis), KA3 (Bacillus paramycoides), and KA10 (Escherichia coli). Indigenous bacteria from the waters of Ambon Bay have the potential to degrade LDPE microplastics, which causes structural changes, decreased crystallinity, weight, and C=C groups in microplastics after degradation, with bacterial isolate KA1 identified as Bacillus cereus showing the best potential with degradation of LDPE microplastics by 36.19%.

Characterization of Batik Waste Containing Synthetic Textile Dyes and The Artisan Awareness Regarding The Hazardous Batik Waste Hidayati, Yunin; Siswanto, Dian; Rumhayati, Barlah; Retnaningdyah, Catur
Biosaintifika: Journal of Biology & Biology Education Vol. 16 No. 2 (2024): August 2024
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v16i2.2325

Synthetic textile dyes in the Tanjung Bumi batik coloring process in Madura, Indonesia, have been used extensively for decades, raising environmental concerns due to hazardous wastewater. This research aims to evaluate the quality characteristics of synthetic textile dye wastewater, assess batik artisans' awareness of batik waste hazards, and analyze the interaction between these factors. Wastewater samples were collected from four locations with two repetitions each. Thirty-seven batik artisans participated in a survey. The wastewater analysis included measurements of BOD5, COD, pH, TSS, phenol, Cr, NH3-N, sulfide, and oil and grease. The survey assessed the artisans' use of synthetic dyes and their awareness of environmental hazards. The results showed that the toxic substance levels in Tanjung Bumi batik waste exceed the quality standard thresholds for textile waste. Survey findings indicate that 100% of respondents use synthetic textile dyes, 83.78% dispose of waste without processing, and 91.89% are unaware of the hazards posed by synthetic textile dyes. There is a direct correlation between the artisans' low awareness of the negative impacts of waste and their lack of environmental preservation practices. The novelty of this research lies in its dual evaluation of chemical wastewater characteristics and socio-environmental awareness among artisans. This approach highlights the urgent need for educational interventions and improved waste management practices. The implications are significant for developing sustainable batik production methods and enhancing environmental awareness, contributing to broader societal benefits.

Phytoplankton as a Bioindicator for Evaluation of the Irrigation Water Quality near a Metal-Coating Factory Nasihah, Mimatun; Ardyati, Ardyati; Siswanto, Dian; Retnaningdyah, Catur
Makara Journal of Science Vol. 28, No. 4
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

The irrigation water quality in Plosowahyu village, Lamongan District, Indonesia, has declined because of industrial waste disposal in irrigation canals. Specifically, waste from a metal-coating factory considerably threatens the aquatic ecosystem. To assess the impact, we evaluated water quality on the basis of phytoplankton diversity and various phys-icochemical parameters. We collected water and phytoplankton samples from four locations in Plosowahyu village: before the wastewater treatment plant (WWTP) outlet of the metal-coating factory, at this outlet, and from two sites in Wahyu River, with three repetitions at each site. The study measured several physicochemical parameters, including total dissolved solids (TDS), total suspended solids, conductivity, temperature, pH, salinity, dissolved oxygen (DO), bio-logical oxygen demand (BOD), chemical oxygen demand (COD), nitrate, orthophosphate, bicarbonate, total organic matter, sodium adsorption ratio, and lead (Pb) concentration. Phytoplankton diversity, indicated by the Shannon–Wiener index, suggested that water quality was moderately polluted at the locations before and at the WWTP outlet, lightly polluted at Wahyu River 2, and clean at Wahyu River 1. Physicochemical tests revealed that irrigation water quality parameters were below Indonesian government standards at several locations: TDS at the WWTP outlet of the metal-coating factory, DO at Wahyu River 1 and Wahyu River 2, and COD at the WWTP outlet and both Wahyu Riv-er locations. BOD, nitrate, bicarbonate, and Pb concentrations were below standards at all study sites. Principal compo-nent analysis confirmed that the metal-coating factory considerably degrades water quality. A comprehensive irriga-tion water management plan must be implemented to protect and improve irrigation water quality in Plosowahyu vil-lage.

The The Effect of Gamma Irradiation on In Vitro Explant Growth, Shoot Multiplication, and Plantlet Regeneration Explants Bulbil of Porang (Amorphophallus muelleri Blume) Ni'amul Albab, Muhammad; Widoretno, Wahyu; Siswanto, Dian; Waluyo, Budi; Mertasari, Chaerani
The Journal of Experimental Life Science Vol. 15 No. 1 (2025)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2025.015.01.06

Porang (Amorphophallus muelleri Blume) is a tuberous plant that contains glucomannan as an alternative in reducing obesity. It is widely traded, but it is cultivated through conventional methods and takes a long time. In vitro mutagenesis technique with gamma irradiation is expected to increase the production of superior porang. This study aims to determine the effect of gamma irradiation on explant growth, shoot multiplication, and plantlet regeneration on in vitro culture. The doses of gamma irradiation, i.e. 0, 2, 4, 6, 8, and 10 gamma rays (Gy). Explants bulbil were precultured for 14 days on Murashige and Skoog (MS) medium fortified with 6-benzyl amino purine (BAP) 3 mg.L-1 before irradiation. The irradiated explants were then cultured on media containing MS supplemented with α-naphthalene acetic acid (NAA) 0.1 mg.L-1 and BAP 3 mg.L-1 for 4 and 8 weeks of culture. Plantlet regeneration media was MS supplemented with NAA 0.1 mg.L-1 for 4 weeks of culture. The results showed that a dose of 10 Gy gamma irradiation was able to inhibit explant growth (0.06 g), shoot multiplication (0%), and plantlet height (0.38 cm), while gamma irradiation at a dose of 6 Gy increased explant growth (0.16 g), shoot multiplication (40%), and plantlet height (5.60 cm). The lethal dose 50 (LD50) of gamma irradiation for explant survival rate is 8.79 Gy. Keywords: Amorphophallus muelleri, explant growth, gamma irradiation, in vitro, plantlet regeneration, shoot multiplication

Inhibitory Effect of Aqueous Garlic (Allium sativum) Bulb Extract on Growth and Physiological Response Dynamics of Two Palm Species Setiyanto, Afif Eka Rahma; Nashafi, Azizuddin Muhammad; Turhadi, Turhadi; Siswanto, Dian
Biosaintifika: Journal of Biology & Biology Education Vol. 17 No. 2 (2025): August 2025
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v17i2.22108

The Merrill palm (Adonidia merrillii) and the Yellow palm (Dypsis lutescens) are hosts of various pests. Garlic (Allium sativum) phytochemicals are applied as a phytopesticide and affect the plant host as well. On the other hand, studies related to garlic phytochemicals' effect on these palms are limited. The present study was conducted to explore the effect of aqueous garlic bulb extract (AGBE) on the Merrill palm and the Yellow palm on those physiological dynamics and growth responses to apply the best concentration as a phytopesticide and observe the palm performance. AGBE is conducted by maceration and application by spraying. Variation AGBE concentrations were 12.5 g/100 mL, 25 g/100 mL, and 50 g/100 mL. The results revealed the profiles of the two palms were different, causing significant differences in growth, in which the Merrill palm was taller while Yellow palm greater leaf number increase; physiological response of two palms was not differing significantly on carbon dioxide concentration but differ significantly on stomatal opening percentage in 28 DAS (day after spraying) and chlorophyll content in 56 DAS; AGBE 25 g/100 mL suppress height increase significantly but leaf number increase not affected; AGBE did not influenced significantly on carbon dioxide, AGBE 50 g/100 mL significantly influenced on stomatal opening in 42 and 56 DAS but chlorophyll not significantly different, overall AGBE tend to inhibit all physiological response. This study concludes that AGBE concentrations in the current study are not recommended as a phytopesticide on both palms due to all concentrations generally inhibiting the growth and physiological response of these palms.

Variation of Fruit Color in Cakra Hijau, G1/M8 and HV-149 Chilli Pepper Cultivar: Physiology and Molecular Approach: Variation of Fruit Color in Cakra Hijau, G1/M8 and HV-149 Cultivar Arumingtyas, Estri Laras; Zakiyah, 'Ainun Sayyidah; Monica, Rosina; Siswanto, Dian
Journal of Tropical Life Science Vol. 13 No. 3 (2023)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.13.03.13

The fruit color of chili pepper is an important characteristic in identification and classification and is often used as the basis for determining consumer preferences. Information on the relationship between chili fruit color and its molecular profile is very important in supporting selection activities in plant breeding. This study aims to identify genetic diversity associated with the fruit color of three genotypes of chili (Capsicum frutescens L.): Cakra Hijau, HV-149 and G1/M8, using Random Amplified Polymorphic DNA (RAPD) and Simple Sequence Repeat (SSR). Morphological confirmation was carried out according to Capsicum descriptors. Nineteen RAPD markers and six SSR markers were used for genetic variability assessment. Genetic variation was analyzed using the unweighted pair group method with the arithmetic mean and the Jaccard similarity index. The three chili genotypes had different fruit colors at each maturation stage. The immature Cakra Hijau fruit is dark green and turns dark red as it ripens. The immature fruits of the G1/M8 line are light green and turn red when ripe. Finally, immature HV-149 fruits are dark green and yellow when ripe. The SSR markers used in this study were unable to show polymorphism. On the other hand, the RAPD marker successfully detected genetic variation in the three chili genotypes and resulted in a total of 49 alleles. The average value of polymorphic information content of the RAPD primers used ranged from 0 to 0.296, with the highest index indicated by OPA-1. The dendrogram shows the separation of the three genotypes into two main clusters, with the first cluster consisting of the HV-149 variety and the second cluster consisting of Cakra Hijau and G1/M8 lines. This study revealed that there are genetic variations based on the morphological characteristics of fruit color at each ripening stage and RAPD band profile. The RAPD marker was more effective than the SSR marker for identifying the genetic diversity of fruit color in the three chilies studied.

Molecular Interaction of Purple Rice Ferulic Acid and Skin Aging-Related Enzymes Wijayanti, Ernanin Dyah; Fatchiyah, Fatchiyah; Safitri, Anna; Siswanto, Dian
Makara Journal of Science Vol. 29, No. 1
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Purple rice is rich in ferulic acid, a phenolic acid abundant in rice. Ferulic acid (FA) has long been recognized for its anti-oxidant properties, which are linked to its skin anti-aging activity owing to its ability to scavenge free radicals and pro-tect cells from damage. Skin anti-aging activity could be assessed by inhibiting skin aging enzymes. However, there was limited information on the mechanism of FA inhibition of these enzymes. This study focused on the molecular interac-tion between purple rice FA and collagenase or tyrosinase, both with and without their substrates. The interaction be-tween FA (CID 445858), collagenase (2Y6I), tyrosinase (5M8M), collagenase substrate (CID 5486160), and tyrosinase substrate (CID 6057) was docked using Hex 8.0.0 Cuda with the default settings. Discovery Studio 2020 and LigPlot software were used to visualize the docking results. Ascorbic acid (CID 54670067) and kojic acid (CID 3840) were used as positive controls for collagenase and tyrosinase, respectively. Molecular docking revealed that FA formed electro-static, hydrogen, and hydrophobic bonds with all aging enzymes, substrates, and enzyme–substrate complexes. FA could bind to both free enzymes and free substrates, consequently attenuating enzyme–substrate bonding, and preventing the substrate from binding appropriately in its normal position. Molecular dynamics simulations demonstrated that FA formed a stable bond with tyrosinase, similar to kojic acid, although the bond with collagenase was less stable. However, the pattern of each parameter during the simulation was comparable with that of ascorbic acid. The current study indicated that the FA from purple rice had the potential to act as an inhibitor of skin aging en-zymes and could be developed as a skin anti-aging candidate

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