Article Per Year (5 Year)
p-Index From 2021 - 2026
2.926
P-Index
This Author published in this journals
All Journal HAYATI Journal of Biosciences Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences The Journal of Experimental Life Sciences (JELS) Biotropika Natural B AGRIVITA, Journal of Agricultural Science Jurnal Penelitian Pendidikan IPA (JPPIPA) Berkala Penelitian Hayati Bioscientiae, Jurnal ilmu ilmu Biologi Makara Journal of Science Jurnal Pengabdian Masyarakat Biologi dan Sains Jurnal Pendidikan IPA Indonesia
Dian Siswanto
Department Of Biology, Faculty Of Mathematics And Natural Science, Universitas Brawijaya, Malang, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Education Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Physics Veterinary

Published : 23 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 23 Documents
Search

 1   2   3 
Characterization of Microplastic Degrading Indigenous Bacteria from Ambon Bay Waters Zakaria, Idham Halil; Siswanto, Dian; Mustafa, Irfan
HAYATI Journal of Biosciences Vol. 31 No. 5 (2024): September 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.5.867-879

Abstract

Microplastic degradation by bacteria can degrade low-density polyethylene (LDPE). This study aimed to analyze the potential of Ambon Bay bacteria for microplastic degradation, the condition of microplastics after degradation, and identification of the potential for microplastic degradation. The results of isolation revealed as many as 20 bacterial isolates, which correlated with physicochemical conditions in the waters of Ambon Bay. Nine of them could degrade microplastics as indicated by the presence of a clear zone, namely KA1, KA2, KA3, KA4, KA5, KA9, KA10, KS6, and KS8. They were checked for biofilm formation, microplastic hydrophobicity, and percentage of microplastic weight reduction. Four isolates with the highest percentage of microplastic weight reduction on day 40 were KA1, KA2, KA3, and KA10 at 36.19%, 10.16%, 28.39%, and 17.07%, respectively. The results of LDPE microplastic degradation showed differences using field emission scanning electron microscopy-energy dispersive spectroscopy (FESEM/EDS), attenuated total reflection-fourier transform infrared (ATR-FTIR), and X-ray diffraction (XRD). The bacterial isolates identified were KA1 (Bacillus cereus), KA2 (Bacillus toyonesis), KA3 (Bacillus paramycoides), and KA10 (Escherichia coli). Indigenous bacteria from the waters of Ambon Bay have the potential to degrade LDPE microplastics, which causes structural changes, decreased crystallinity, weight, and C=C groups in microplastics after degradation, with bacterial isolate KA1 identified as Bacillus cereus showing the best potential with degradation of LDPE microplastics by 36.19%.
Phytoplankton as a Bioindicator for Evaluation of the Irrigation Water Quality near a Metal-Coating Factory Nasihah, Mimatun; Ardyati, Ardyati; Siswanto, Dian; Retnaningdyah, Catur
Makara Journal of Science Vol. 28, No. 4
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The irrigation water quality in Plosowahyu village, Lamongan District, Indonesia, has declined because of industrial waste disposal in irrigation canals. Specifically, waste from a metal-coating factory considerably threatens the aquatic ecosystem. To assess the impact, we evaluated water quality on the basis of phytoplankton diversity and various phys-icochemical parameters. We collected water and phytoplankton samples from four locations in Plosowahyu village: before the wastewater treatment plant (WWTP) outlet of the metal-coating factory, at this outlet, and from two sites in Wahyu River, with three repetitions at each site. The study measured several physicochemical parameters, including total dissolved solids (TDS), total suspended solids, conductivity, temperature, pH, salinity, dissolved oxygen (DO), bio-logical oxygen demand (BOD), chemical oxygen demand (COD), nitrate, orthophosphate, bicarbonate, total organic matter, sodium adsorption ratio, and lead (Pb) concentration. Phytoplankton diversity, indicated by the Shannon–Wiener index, suggested that water quality was moderately polluted at the locations before and at the WWTP outlet, lightly polluted at Wahyu River 2, and clean at Wahyu River 1. Physicochemical tests revealed that irrigation water quality parameters were below Indonesian government standards at several locations: TDS at the WWTP outlet of the metal-coating factory, DO at Wahyu River 1 and Wahyu River 2, and COD at the WWTP outlet and both Wahyu Riv-er locations. BOD, nitrate, bicarbonate, and Pb concentrations were below standards at all study sites. Principal compo-nent analysis confirmed that the metal-coating factory considerably degrades water quality. A comprehensive irriga-tion water management plan must be implemented to protect and improve irrigation water quality in Plosowahyu vil-lage.
The The Effect of Gamma Irradiation on In Vitro Explant Growth, Shoot Multiplication, and Plantlet Regeneration Explants Bulbil of Porang (Amorphophallus muelleri Blume) Ni'amul Albab, Muhammad; Widoretno, Wahyu; Siswanto, Dian; Waluyo, Budi; Mertasari, Chaerani
The Journal of Experimental Life Science Vol. 15 No. 1 (2025)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2025.015.01.06

Abstract

Porang (Amorphophallus muelleri Blume) is a tuberous plant that contains glucomannan as an alternative in reducing obesity. It is widely traded, but it is cultivated through conventional methods and takes a long time. In vitro mutagenesis technique with gamma irradiation is expected to increase the production of superior porang. This study aims to determine the effect of gamma irradiation on explant growth, shoot multiplication, and plantlet regeneration on in vitro culture. The doses of gamma irradiation, i.e. 0, 2, 4, 6, 8, and 10 gamma rays (Gy). Explants bulbil were precultured for 14 days on Murashige and Skoog (MS) medium fortified with 6-benzyl amino purine (BAP) 3 mg.L-1 before irradiation. The irradiated explants were then cultured on media containing MS supplemented with α-naphthalene acetic acid (NAA) 0.1 mg.L-1 and BAP 3 mg.L-1 for 4 and 8 weeks of culture. Plantlet regeneration media was MS supplemented with NAA 0.1 mg.L-1 for 4 weeks of culture. The results showed that a dose of 10 Gy gamma irradiation was able to inhibit explant growth (0.06 g), shoot multiplication (0%), and plantlet height (0.38 cm), while gamma irradiation at a dose of 6 Gy increased explant growth (0.16 g), shoot multiplication (40%), and plantlet height (5.60 cm). The lethal dose 50 (LD50) of gamma irradiation for explant survival rate is 8.79 Gy. Keywords: Amorphophallus muelleri, explant growth, gamma irradiation, in vitro, plantlet regeneration, shoot multiplication
Variation of Fruit Color in Cakra Hijau, G1/M8 and HV-149 Chilli Pepper Cultivar: Physiology and Molecular Approach: Variation of Fruit Color in Cakra Hijau, G1/M8 and HV-149 Cultivar Arumingtyas, Estri Laras; Zakiyah, 'Ainun Sayyidah; Monica, Rosina; Siswanto, Dian
Journal of Tropical Life Science Vol. 13 No. 3 (2023)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.13.03.13

Abstract

The fruit color of chili pepper is an important characteristic in identification and classification and is often used as the basis for determining consumer preferences. Information on the relationship between chili fruit color and its molecular profile is very important in supporting selection activities in plant breeding. This study aims to identify genetic diversity associated with the fruit color of three genotypes of chili (Capsicum frutescens L.): Cakra Hijau, HV-149 and G1/M8, using Random Amplified Polymorphic DNA (RAPD) and Simple Sequence Repeat (SSR). Morphological confirmation was carried out according to Capsicum descriptors. Nineteen RAPD markers and six SSR markers were used for genetic variability assessment. Genetic variation was analyzed using the unweighted pair group method with the arithmetic mean and the Jaccard similarity index. The three chili genotypes had different fruit colors at each maturation stage. The immature Cakra Hijau fruit is dark green and turns dark red as it ripens. The immature fruits of the G1/M8 line are light green and turn red when ripe. Finally, immature HV-149 fruits are dark green and yellow when ripe. The SSR markers used in this study were unable to show polymorphism. On the other hand, the RAPD marker successfully detected genetic variation in the three chili genotypes and resulted in a total of 49 alleles. The average value of polymorphic information content of the RAPD primers used ranged from 0 to 0.296, with the highest index indicated by OPA-1. The dendrogram shows the separation of the three genotypes into two main clusters, with the first cluster consisting of the HV-149 variety and the second cluster consisting of Cakra Hijau and G1/M8 lines. This study revealed that there are genetic variations based on the morphological characteristics of fruit color at each ripening stage and RAPD band profile. The RAPD marker was more effective than the SSR marker for identifying the genetic diversity of fruit color in the three chilies studied.
Molecular Interaction of Purple Rice Ferulic Acid and Skin Aging-Related Enzymes Wijayanti, Ernanin Dyah; Fatchiyah, Fatchiyah; Safitri, Anna; Siswanto, Dian
Makara Journal of Science Vol. 29, No. 1
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Purple rice is rich in ferulic acid, a phenolic acid abundant in rice. Ferulic acid (FA) has long been recognized for its anti-oxidant properties, which are linked to its skin anti-aging activity owing to its ability to scavenge free radicals and pro-tect cells from damage. Skin anti-aging activity could be assessed by inhibiting skin aging enzymes. However, there was limited information on the mechanism of FA inhibition of these enzymes. This study focused on the molecular interac-tion between purple rice FA and collagenase or tyrosinase, both with and without their substrates. The interaction be-tween FA (CID 445858), collagenase (2Y6I), tyrosinase (5M8M), collagenase substrate (CID 5486160), and tyrosinase substrate (CID 6057) was docked using Hex 8.0.0 Cuda with the default settings. Discovery Studio 2020 and LigPlot software were used to visualize the docking results. Ascorbic acid (CID 54670067) and kojic acid (CID 3840) were used as positive controls for collagenase and tyrosinase, respectively. Molecular docking revealed that FA formed electro-static, hydrogen, and hydrophobic bonds with all aging enzymes, substrates, and enzyme–substrate complexes. FA could bind to both free enzymes and free substrates, consequently attenuating enzyme–substrate bonding, and preventing the substrate from binding appropriately in its normal position. Molecular dynamics simulations demonstrated that FA formed a stable bond with tyrosinase, similar to kojic acid, although the bond with collagenase was less stable. However, the pattern of each parameter during the simulation was comparable with that of ascorbic acid. The current study indicated that the FA from purple rice had the potential to act as an inhibitor of skin aging en-zymes and could be developed as a skin anti-aging candidate
Ethnobotanical Insights into Plant Diversity Along the Ecotourism Corridor of Mount Prau in Purwosari Village, Indonesia Abdullah, Muhammad; Hakim, Luchman; Siswanto, Dian; Liesnoor Setyowati, Dewi
Jurnal Pendidikan IPA Indonesia Vol. 13 No. 3 (2024): September 2024
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/v30vry34

Abstract

Examining ethnobotany in mountainous regions established as popular hiking destinations is essential for conservation initiatives. In this study, we aim to explore the ethnobotanical knowledge of the indigenous communities and to analyze the plant diversity in the ecotourism corridor to Mount Prau in Purwosari Village, Central Java, Indonesia. Ethnobotanical data was collected through semi-structured interviews with informants recruited via purposive sampling. An exploratory approach was used to inventory plant diversity along the ecotourism corridor of Mount Prau in Purwosari village. Our key informants comprised village officials, community leaders, Mount Prau caretakers, and basecamp managers. The findings indicated that the informants explained a total of 68 plant species, which were utilized by the community for various purposes. These plants served as valuable food sources, medicinal ingredients, and economic value, and they were also used for fodder, building materials, shade, and conservation purposes. In the ecotourism corridor in Purwosari village, which serves as the conservation trail for Mount Prau, a total of 117 plant species were identified through an inventory in this study. Finding some invasive alien plant species, such as Acacia decurens and Clidemia hirta, and an endangered species, Castanopsis argentea, highlights the importance of conservation efforts for protecting and preserving the diverse plant species found along the conservation trail to Mount Prau.
Genomic Profile of OsCOMT in Indonesian Purple Rice Wijayanti, Ernanin Dyah; Safitri, Anna; Siswanto, Dian; Fatchiyah, Fatchiyah
Biotropika: Journal of Tropical Biology Vol. 10 No. 3 (2022)
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.biotropika.2022.010.03.04

Abstract

The Indonesian purple rice (IPR), a crossbreeding of black and white rice, is a potential source of ferulic acid. Up to this point, the genomic similarity between the crossbreeding and its parentals has not been determined, particularly in genes involved in ferulic acid biosynthesis. In this present work, we analysed the profile of Oryza sativa caffeic acid-O-methyltransferase (OsCOMT) gene in IPR. The genomic DNA was extracted by CTAB method, then amplified with a specific primer of OsCOMT gene. The amplicon was sequenced by Sanger method and confirmed by geneID NC_029259.1. The IPR showed an amplicon of 708 bp sequence of the OsCOMT gene, indicating the gene involved in ferulic acid biosynthesis. The IPR gene profile is similar to the parentals, but there are variations in point mutations that distinguish it from the parentals. Aspartic acid was changed to Glutamine by mutations at positions 52, 69, and 79. We suggest that IPR has a novel OsCOMT gene variant that is unique as it is the crossbreed.
Biosistem Pertanian Apel Lokal Malang Siswanto, Dian; Mustafa, Irfan; Ekowati, Gustini; Imam, Muhamad; Purnomo, Endri
The Journal of Experimental Life Science Vol. 1 No. 2 (2011)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2011.001.02.06

Abstract

Biosistem yang digambarkan pada hasil observasi di sini adalah suatu keadaan faktor biotik dan abiotik pada pertanian apel yang tidak terbatas pada suatu nilai yang tetap. Data  yang diperoleh dari tiga tahapan penelitian ini diharapkan dapat dipergunakan sebagai informasi yang mendukung upaya konsevasi pertanian apel. Tahapan penelitian tersebut meliputi evaluasi nutrisi makro tanah dan tanaman, formulasi pengomposan kotoran sapi diperkaya (menggunakan bakteri indigenus) dan eksplorasi tanaman penutup tanah pertanian apel. Kandungan beberapa nutrisi makro untuk tanah pertanian apel Bumiaji menunjukkan nilai rata-rata yang berada pada kisaran sedang dan tinggi. Hasil analisis makro nutrisi daun apel yang kemudian dibandingkan dengan standar menurut Utah Fertilizer Guide menunjukkan bahwa makro nutrisi N, P, Ca berada pada status normal, K berada pada status kurang dan Mg berlebih. Ketersediaan nutrisi di tanah dapat dipelihara dengan penambahan kompos. Kotoran sapi yang diperkaya dengan bakteri indigen mampu mendekomposisi kotoran sapi dalam waktu 2-3 minggu. Proses dekomposisi kotoran sapi berlangsung lebih cepat pada pemberian bakteri indigenus yang mempunyai kemampuan selulolitik dan sekaligus proteolitik serta amilolitik apabila dibandingkan kontrol yang tanpa pemberian inokulum bakteri. Pada pertanian apel di Poncokusumo, kondisi lahan dengan sistem penyiangan cs (cukup sering) dan sj (sangat jarang) tampak lebih stabil daripada ss (sering sekali) berdasarkan nilai ID (indek diversitas), DR (dominansi relatif) dan INP (indek nilai penting). Hal ini diperjelas oleh nilai biomassa tanaman. Biomassa yang lebih tinggi menyebabkan suhu tanah yang lebih rendah karena jumlah TPT yang banyak mempunyai konsekuensi mengandung banyak air pada jaringannya. Kata kunci: Apel, nutrisi makro, kompos diperkaya, tanaman penutup tanah
Antifeedant Effect of Moringa oleifera (L.) Leaf and Seed Extract on Growth and Feeding Activity of Spodoptera litura (Fab.) (Lepidoptera: Noctuidae) Tridiptasari, Amelia; Leksono, Amin Setyo; Siswanto, Dian
The Journal of Experimental Life Science Vol. 9 No. 1 (2019)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1061.918 KB) | DOI: 10.21776/ub.jels.2019.009.01.05

Abstract

This study was conducted to evaluate the effect of Moringa oleifera leaf and seed extract on length, weight, and feeding activity of Spodoptera litura. Leaf and seed were extracted by maceration method in 70% ethanol. Bioassays were conducted by using five concentrations (0%, 5%, 10%, 15%, 20%) on 5 larvae by leaf-dip method with 5 times replication. The result showed that leaf and seed of Moringa extract contained alkaloid, flavonoid, and saponin which gave antifeedant effect to growth and feeding activity of larvae. The increased extract concentrations are lead to weight loss and the length shrinkage of larvae. The extracts promoted prolongation of the larval instar. Reduction of food intake of the larva is in line with the concentration increase of extracts. The antifeedant effect also affects time to stop feeding and give mortality of larvae.Keywords: antifeedant, leaf, moringa, seed, S. litura
The Sensitivity of Leersia hexandra Sw. to Gamma-Ray Irradiation Sukmasari, Pritha Kartika; Widoretno, Wahyu; Siswanto, Dian
The Journal of Experimental Life Science Vol. 11 No. 1 (2021)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2021.011.01.02

Abstract

Gamma-ray irradiation as a physical mutagen has high penetrating power. Therefore, it is most often used to increase genetic variability or produce new mutant plants. This research was conducted to obtain the lethal dose of gamma-rays in Leersia hexandra plants. The used plant part was a single node stolon which had a length of 10 cm with the node in the middle of the stolon. The irradiation doses given were 0, 25, 50, 75, 100 Gy. Stolons were inserted into plastic clips and irradiated using a Cobalt-60 gamma irradiation source at the Center for Irradiation and Radioisotope Applications (PAIR), National Nuclear Energy Agency (BATAN) Jakarta. Gamma-irradiation has a significant effect on inhibiting shoot growth. The growth of irradiated Leersia hexandra with the best number of plants and the highest shoots was obtained at a dose of 25 Gy and decreased with increasing irradiation dose. The lethal dose (LD50) was determined eight days after irradiation using CurveExpert 1.4 software. Leersia hexandra plants that can sprout and regenerate followed the linear equation y = 1.02 - 7.5x with LD50 at 68.85 Gy and LD20 at 29.36 Gy.Keywords: gamma irradiation, genetic variation, Leersia hexandra, lethal dose, mutant plant.
Co-Authors Amin Setyo Leksono Ardyati, Ardyati Aris Soewondo Brian Rahardi Budi Waluyo Catur Retnaningdyah Catur Retnaningdyah Dewi Liesnoor Setyowati Dodyk Pranowo Endri Purnomo Ernanin Dyah Wijayanti Estri Laras Arumingtyas Fadillah Putra fatchiyah . Fatchiyah Fatchiyah Gustini Ekowati Irfan Mustafa Kurniawan Sigit Wicaksono, Kurniawan Sigit Lidwina Faraline Triprisila Luchman Hakim M. Halim Natsir Makagoshi, Nobukazu Mertasari, Chaerani Monica, Rosina Muhamad Imam Muhammad Abdullah Nasihah, Mimatun Neda Prasiska Kusumawati Ni'amul Albab, Muhammad Oraby, Ghada Abd Elsattar Mohammed Safitri, Anna Sahiruddin Sahiruddin Serafinah Indriyani Sofy Permana Sri Widyarti Suharjono Suharjono Sukmasari, Pritha Kartika Suksabye, Parinda Thiravetyan, Paitip Tri Ardyati Tridiptasari, Amelia Umi Sa'adah Uswatun Hasanah Wahyu Widoretno Wresti L. Anggayasti Zakaria, Idham Halil Zakiyah, 'Ainun Sayyidah