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All Journal HAYATI Journal of Biosciences Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences The Journal of Experimental Life Sciences (JELS) Biotropika Natural B Jurnal Penelitian Pendidikan IPA (JPPIPA) Berkala Penelitian Hayati Bioscientiae, Jurnal ilmu ilmu Biologi Makara Journal of Science Jurnal Pengabdian Masyarakat Biologi dan Sains Biosaintifika: Journal of Biology & Biology Education Jurnal Pendidikan IPA Indonesia
Dian Siswanto
Department Of Biology, Faculty Of Mathematics And Natural Science, Universitas Brawijaya, Malang, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Education Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Physics Veterinary

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Biosistem Pertanian Apel Lokal Malang Siswanto, Dian; Mustafa, Irfan; Ekowati, Gustini; Imam, Muhamad; Purnomo, Endri
The Journal of Experimental Life Science Vol. 1 No. 2 (2011)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2011.001.02.06

Abstract

Biosistem yang digambarkan pada hasil observasi di sini adalah suatu keadaan faktor biotik dan abiotik pada pertanian apel yang tidak terbatas pada suatu nilai yang tetap. Data  yang diperoleh dari tiga tahapan penelitian ini diharapkan dapat dipergunakan sebagai informasi yang mendukung upaya konsevasi pertanian apel. Tahapan penelitian tersebut meliputi evaluasi nutrisi makro tanah dan tanaman, formulasi pengomposan kotoran sapi diperkaya (menggunakan bakteri indigenus) dan eksplorasi tanaman penutup tanah pertanian apel. Kandungan beberapa nutrisi makro untuk tanah pertanian apel Bumiaji menunjukkan nilai rata-rata yang berada pada kisaran sedang dan tinggi. Hasil analisis makro nutrisi daun apel yang kemudian dibandingkan dengan standar menurut Utah Fertilizer Guide menunjukkan bahwa makro nutrisi N, P, Ca berada pada status normal, K berada pada status kurang dan Mg berlebih. Ketersediaan nutrisi di tanah dapat dipelihara dengan penambahan kompos. Kotoran sapi yang diperkaya dengan bakteri indigen mampu mendekomposisi kotoran sapi dalam waktu 2-3 minggu. Proses dekomposisi kotoran sapi berlangsung lebih cepat pada pemberian bakteri indigenus yang mempunyai kemampuan selulolitik dan sekaligus proteolitik serta amilolitik apabila dibandingkan kontrol yang tanpa pemberian inokulum bakteri. Pada pertanian apel di Poncokusumo, kondisi lahan dengan sistem penyiangan cs (cukup sering) dan sj (sangat jarang) tampak lebih stabil daripada ss (sering sekali) berdasarkan nilai ID (indek diversitas), DR (dominansi relatif) dan INP (indek nilai penting). Hal ini diperjelas oleh nilai biomassa tanaman. Biomassa yang lebih tinggi menyebabkan suhu tanah yang lebih rendah karena jumlah TPT yang banyak mempunyai konsekuensi mengandung banyak air pada jaringannya. Kata kunci: Apel, nutrisi makro, kompos diperkaya, tanaman penutup tanah
Antifeedant Effect of Moringa oleifera (L.) Leaf and Seed Extract on Growth and Feeding Activity of Spodoptera litura (Fab.) (Lepidoptera: Noctuidae) Tridiptasari, Amelia; Leksono, Amin Setyo; Siswanto, Dian
The Journal of Experimental Life Science Vol. 9 No. 1 (2019)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1061.918 KB) | DOI: 10.21776/ub.jels.2019.009.01.05

Abstract

This study was conducted to evaluate the effect of Moringa oleifera leaf and seed extract on length, weight, and feeding activity of Spodoptera litura. Leaf and seed were extracted by maceration method in 70% ethanol. Bioassays were conducted by using five concentrations (0%, 5%, 10%, 15%, 20%) on 5 larvae by leaf-dip method with 5 times replication. The result showed that leaf and seed of Moringa extract contained alkaloid, flavonoid, and saponin which gave antifeedant effect to growth and feeding activity of larvae. The increased extract concentrations are lead to weight loss and the length shrinkage of larvae. The extracts promoted prolongation of the larval instar. Reduction of food intake of the larva is in line with the concentration increase of extracts. The antifeedant effect also affects time to stop feeding and give mortality of larvae.Keywords: antifeedant, leaf, moringa, seed, S. litura
The Sensitivity of Leersia hexandra Sw. to Gamma-Ray Irradiation Sukmasari, Pritha Kartika; Widoretno, Wahyu; Siswanto, Dian
The Journal of Experimental Life Science Vol. 11 No. 1 (2021)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2021.011.01.02

Abstract

Gamma-ray irradiation as a physical mutagen has high penetrating power. Therefore, it is most often used to increase genetic variability or produce new mutant plants. This research was conducted to obtain the lethal dose of gamma-rays in Leersia hexandra plants. The used plant part was a single node stolon which had a length of 10 cm with the node in the middle of the stolon. The irradiation doses given were 0, 25, 50, 75, 100 Gy. Stolons were inserted into plastic clips and irradiated using a Cobalt-60 gamma irradiation source at the Center for Irradiation and Radioisotope Applications (PAIR), National Nuclear Energy Agency (BATAN) Jakarta. Gamma-irradiation has a significant effect on inhibiting shoot growth. The growth of irradiated Leersia hexandra with the best number of plants and the highest shoots was obtained at a dose of 25 Gy and decreased with increasing irradiation dose. The lethal dose (LD50) was determined eight days after irradiation using CurveExpert 1.4 software. Leersia hexandra plants that can sprout and regenerate followed the linear equation y = 1.02 - 7.5x with LD50 at 68.85 Gy and LD20 at 29.36 Gy.Keywords: gamma irradiation, genetic variation, Leersia hexandra, lethal dose, mutant plant.
Identification of Sago-Pulp Amylolytic Bacteria and Its Utilization for Granulated Fertilizer Hasanah, Uswatun; Ardyati, Tri; Siswanto, Dian
The Journal of Experimental Life Science Vol. 11 No. 2 (2021)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2021.011.02.02

Abstract

Previous research stated that bacteria isolated from sago waste from the traditional sago industry in Palopo had the potency to produce amylase. This study aims to confirm the ability of bacteria isolated from sago pulp waste to produce amylase, evaluate the ability of these amylolytic bacteria to produce IAA fix nitrogen, identify selected bacteria, and apply selected bacteria into granules biofertilizers. Bacteria were isolated from sago pulp waste and grown on a 1% starch agar medium. The amylolytic activity was analyzed qualitatively using iodine and quantitatively using the 3,5-Dinitrosalicylic Acid (DNS) method at pH 6. Amylolytic bacteria were analyzed for IAA production using Salkowsky reagent and nitrogen fixation ability through the Serra Kit method. Potential bacteria were identified based on the similarity of the 16S rDNA sequence. The selected bacteria were grown in a 10mL NB medium, and then bacteria were inoculated and air-dried to obtain the granules. The selected bacteria were put into granules and re-isolated using the total plate count (TPC) method. Eight amylolytic bacteria were obtained from sago pulp waste. L1E isolate had the highest amylolytic activity of 1.228 U.mL-1, and L1D isolate had the highest IAA production of 69.8 g.mL-1. Isolate L1E was identified as Alcaligenes faecalis with a 99.45% similarity index, and L1D was identified as Serratia surfactantfaciens with a 99.09% similarity index. Isolate L2G was identified as Alcaligenes aquatilis with a similarity index of 99.8% of the 16S rDNA sequence. The viability of bacteria from granules was 1.41 x 108 CFU.g-1.Keywords: Amylase enzyme, amylolytic bacteria, granulated fertilizer.
Co-Authors Amin Setyo Leksono Anggayasti, Wresti L. Ardyati, Ardyati Aris Soewondo Barlah Rumhayati Brian Rahardi Budi Waluyo Catur Retnaningdyah Catur Retnaningdyah Dewi Liesnoor Setyowati Dodyk Pranowo Endri Purnomo Ernanin Dyah Wijayanti Estri Laras Arumingtyas fatchiyah . Fatchiyah Fatchiyah Gustini Ekowati Irfan Mustafa Kurniawan Sigit Wicaksono, Kurniawan Sigit Kusumawati, Neda Prasiska Lidwina Faraline Triprisila Luchman Hakim Makagoshi, Nobukazu Mertasari, Chaerani Monica, Rosina Muhamad Imam Muhammad Abdullah Nashafi, Azizuddin Muhammad Nasihah, Mimatun Ni'amul Albab, Muhammad Safitri, Anna Sahiruddin Sahiruddin Serafinah Indriyani Setiyanto, Afif Eka Rahma Sofy Permana Sri Widyarti Suharjono Suharjono Sukmasari, Pritha Kartika Suksabye, Parinda Thiravetyan, Paitip Tri Ardyati Tridiptasari, Amelia Turhadi Turhadi Umi Sa'adah Uswatun Hasanah Wahyu Widoretno Yunin Hidayati Zakaria, Idham Halil Zakiyah, 'Ainun Sayyidah