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All Journal MEDIA PETERNAKAN - Journal of Animal Science and Technology Jurnal Peternakan Sriwijaya Jurnal Veteriner Jurnal Ilmiah Universitas Batanghari Jambi Animal Production : Indonesian Journal of Animal Production JAS (Jurnal Agri Sains) Jurnal Nukleus Peternakan Semah : Jurnal Pengelolaan Sumberdaya Peraira Jurnal Produksi Ternak Terapan (JPTT) Jurnal Ilmiah Ilmu-Ilmu Peternakan Jurnal Kedokteran Hewan Jurnal Nusantara Mengabdi Journal of Livestock and Animal Health (JLAH)
Bayu Rosadi
Fakultas Peternakan Universitas Jambi
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Computer Science & IT Economics, Econometrics & Finance Education Environmental Science Immunology & microbiology Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Public Health Social Sciences Veterinary Other

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Journal : Jurnal Veteriner

 1 
DEVELOPMENT OF MICE AND HAMSTER EMBRYOS IN KSOMAA AND HECM-6 MEDIUM Bayu Rosadi; M Agus Setiadi; Dondin Sajuthi; Arief Boediono
Jurnal Veteriner Vol 9 No 4 (2008)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The purpose of the present study was to investigate the viability of mice and hamster embryos developed in Kalium Simplex Optimized Medium amino acid (KSOMaa) and Hamster Embryo Culture Medium-6 (HECM-6) medium. Female DDY mice were superovulated by injection i.p. of 5 IU Pregnant Mare Serum Gonadotropine (PMSG) and 5 IU Human Chorionic Gonadotropine (hCG) in 48 h interval, hamster (Phodopus campbelli) injected by 2.5 IU PMSG and 2.5 IU hCG 48 h later. Then females were mated with fertile males. Eight-cell embryos were recovered at day 3 after natural mating. The mice embryos were cultured in KSOMaa+5% NBCS (New Born Calf Serum) (T1) and HECM-6+5% NBCS (T2), the hamster embryos were cultured in KSOMaa+5% NBCS (T3) and HECM-6 + 5% NBCS (T4) for further development at 37oC in a humidified atmosphere of 5% CO2 in air for 48 h. The examinations were replicated five times. The T1 embryos developed to compact morulla and early blastocyst 100% (140/140), 92.1% (129/140) to blastocyst and expanded blastocyst, and 22.9% (32/140) became hatching/hatched. The T3 reached 100% (60/60) to compact morulla and early blastocyst, 85.0% (51/60) blastocyst, and 48.3% (29/60) expanded blastocyst, no embryo observed hatching/hatced. The T2 embryos had more expanded blastocyst than T3 (P<0.05), hatching/hatched rate higher than T1 and T3 but lower than T4 (P<0.05). Shortly, KSOMaa enable to support 8-cell stage mice and hamster embryo, but the hamster embryo developed lower at expanded blastocyst stage. HECM-6 is more appropriate than KSOMaa to support 8-cell mice embryos development and suitable to develop 8-cell stage hamster embryos.
Preservasi Ovarium dan Pengaruhnya Terhadap Morfologi Folikel Domba Bayu Rosadi; Mohamad Agus Setiadi; Dondin Sajuthi; Arief Boediono
Jurnal Veteriner Vol 12, No 2 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The purpose of this study was to evaluate the effect of cooling and freezing of ewes ovarian tissue ontheir follicles morphology. The study was carried out in two experiments. Experiment I, ovaries weremaintained in Phosphate Buffered Saline (PBS) at -20oC and room temperature (RT) for 24 h, and at 5oCfor 24 h and 72 h, respectively. After storage, follicles were histologically evaluated. Experiment II, theovarian cortex was isolated and tissue slices (±1 mm3) were prepared. Following this tissues were loadedinto hemistraw then transferred to equilibration solution (PBS+20% FBS+7,5% EG+7,5 % DMSO) atroom temperature and held for 10, 20, 30 minutes, respectively. Afterward tissues were tranferred tovitrification solution (PBS+20%FBS +15%EG+15%DMSO ) for 3 minutes, then the hemistraw was placeddirectly into liquid nitrogen. After thawing, the tissues were prepared for histological examination. All ofthe follicles were deteriorated after 24 h storage at RT. The percentage of morphologically normal follicleswas significantly reduced when ovarian tissues were stored at -20oC for 24 h and at 5oC for 24 h and 72 h.However, it seemed to have a minor deterioration effect when the tissues were kept at 5oC for 24 h(P<0.05). Antral follicles were damaged in all of the treatments. Primordial follicles preserved theirmorphology intactness better than growing follicles. Exposing tissues to equilibration medium for 10minutes seemed to produce higher numbers of morphologically normal follicles (P<0.05), compared towhen tissues were exposed for 20 minutes and 30 minutes (P>0.05). It can be concluded that exposingtissues to equilibration solution for 10 minutes prior to freezing would kept the ovarian follicles morphologyin good condition.
Identifikasi Gangguan Reproduksi pada Ovarium Sapi Potong yang Mengalami Anestrus Postpartum Panjang (IDENTIFICATION OF REPRODUCTIVE DISRUPTION ON PROLONGED POSTPARTUM ANESTRUS BEEF COW OVARIES) Bayu Rosadi; Teguh Sumarsono; Fachroerrozi Hoesni
Jurnal Veteriner Vol 19 No 3 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Postpartum anestrus is the major factor causing elongation of the calving interval and in consequense, lowering beef cow productivity. Anestrus condition is closey related to inactive ovary condition. The research was conducted to find out reproductive disorders in ovarian of beef cattle cow that underwent prolonged postpartum anestrus (more than 3 months). One hundred and fifty heads post partum anestrus cow were selected. The general body condition were evaluated based on Body Condition Score (BCS). The types of reproductive disruption in ovary were determined by rectal palpation method. The cows observed had 2.0 to 3.2 (2.6 ± 0.7) in BCS. The result showed that 37.68% of prolonged postparum anestrus cow had ovarian disorders. The types of ovarian disoreders detected were hypofunction (19.32%), cystic follicle (8.21%), persistent corpus luteum (4.5%), atrophy (1.93%) and partial agenesis (0.97%).
Co-Authors A. Parakkasi A. Parakkasi Ade Nopianti ADRIANI ADRIANI Afriani H Ahmad Syarifuddin Arief Boediono Awaludin, Aan Bayu Islami Pasyah D Darmawan Damhuri, Dedi Darmawan Darmawan Depison Depison Dondin Sajuthi Endri Musnandar Engki Zelpina Enny T Setiatin Enny T Setiatin, Enny Fachroerrozi Hoesni, Fachroerrozi Farizal Farizal Farizal Farizal Firmansyah Firmansyah Gunawan G Gunawan G, Gunawan Guspianto Guspianto Jalius Jalius Jessica Anggun Safitri Jodion Siburian Liber Hasudungan Sinurat Lusi Amidia M Agus Setiadi Mursalin Mursalin Mustakim Mustakim Nisfu Bayu Kurniadi Nugraheni, Yudhi Ratna Pudji Rahayu Ratna Sholatia Harahap Revy Andryansyah Sarwo Edy Wibowo Silvia Erina Suhardi Pratama Sutrisno Sutrisno Syafril Hadi Taufiq Arrahman Teguh Sumarsono Thomas Mata Hine Trio Mohammad Syafi&#039;i Yatno, Yatno