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All Journal Jurnal Natur Indonesia Jurnal Teknologi Pangan dan Gizi Jurnal Pangan dan Agroindustri agriTECH Indonesian Journal of Biotechnology JITEK (Jurnal Ilmiah Teknosains) JITIPARI (Jurnal Ilmiah Teknologi dan Industri Pangan UNISRI) Indonesian Food and Nutrition Progress Jurnal Teknologi Hasil Pertanian Inovasi Jurnal Pengabdian Masyarakat (IJPM)
Endang Sutriswati Rahayu
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Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Engineering Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mechanical Engineering Public Health Social Sciences Veterinary

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Molecular Identification of Lactic Acid Bacteria Producing Antimicrobial Agents from Bakasang, An Indonesian Traditional Fermented Fish Product Lawalata, Helen Joan; Sembiring, Langkah; Rahayu, Endang Sutriswati
Indonesian Journal of Biotechnology Vol 16, No 2 (2011)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (43.035 KB)

Abstract

AbstractTwenty seven strains of lactic acid bacteria (LAB) were isolated from bakasang, Indonesian traditional fermented fish product. In general, LAB have inhibitory activity againts pathogenic bacteria and spoilage bacteria. Screening for antimicrobia activity of isolates were performed with well-diffusion method. One isolate that was designed as Pediococcus BksC24 was the strongest against bacteria pathogenic and spoilage bacteria. This strain was further identified by 16S rRNA gen sequence comparison. Isolates LAB producing antimicrobial agents from bakasang were identified as Pediococcus acidilactici.Keywords : Bakasang, LAB, antimicrobial, phenotypic characteristics, 16S rRNA gene
PEMANFAATAN HASIL FERMENTASI WHEY TAHU MENGGUNAKAN ISOLAT PEDIOCOCCUS ACIDILACTICI F11 SEBAGAI ALTERNATIF KOAGULAN PADA PEMBUATAN TAHU K. Sya’di, Yunan; Rahayu, Endang Sutriswati; Cahyanto, Muhammad Nur
Jurnal Ilmiah Teknosains Vol 1, No 1/November (2015): JITek
Publisher : Jurnal Ilmiah Teknosains

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Abstract

Pembuatan tahu di Indonesia banyak menggunakan kecutan sebagai penggumpal. Kecutan ini memiliki kelemahan karenajenis dan jumlah mikrobia yang berperan dalam fermentasi dapat berbeda dari satu fermentasi dengan fermentasi lainnya.Akibatnya mutu tahu yang dihasilkan dari proses penggumpalannya juga kurang stabil. Penelitian ini bertujuan menggunakanhasil fermentasi whey tahu dengan bakteri asam laktat, Pediococcus acidilactici F11 sebagai alternatif penggumpal pada skalaindustri. Fermentasi dilakukan pada fermentor 125 L selama 5 hari produksi (5 batch) pada skala industri. Populasi akhirbakteri asam laktat dari 5 batch fermentasi kecutan menggunakan Pediococcus acidilactici F11 pada fermentor 125 liter selama16 jam memiliki rata-rata 1,19 x 109 CFU/mL, sedangkan bakteri non BAL dan coliform memiliki rata-rata 1,8 x 102 CFU/mLdan 8,23 CFU/mL. Keasaman dan pH pada akhir fermentasi kecutan sudah memenuhi persyaratan sebagai penggumpaldengan memiliki rata-rata 3,54 g/L dan pH rata-rata 3,94. Berat tahu yang dihasilkan dari koagulan hasil fermentasi whey tahumenggunakan Pediococcus acidilactici F11 lebih tinggi 5,9% dibanding menggunakan koagulan dari kecutan.
KAJIAN PROPORSI SARI NANAS DAN KONSENTRASI STARTER TERHADAP AKTIVITAS ANTIBAKTERI KEFIR NANAS Mayasari, Dewi; Nugerahani, Ira; Rahayu, Endang Sutriswati
Jurnal Teknologi Pangan dan Gizi Vol 15, No 2 (2016)
Publisher : Widya Mandala Surabaya Catholic University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (351.184 KB) | DOI: 10.33508/jtpg.v15i2.1540

Abstract

This research uses Queen pineapple varieties juice because it have a high sugar content and contains antibacterial compounds such as phenol, chlorine, and iodine. The purpose of this study is to determine the effect of different proportions of pineapple juice and starter concentrations to the antibacterial activity of pineapple kefir. The experimental design that will be use is a Factorial Randomized Block Design (RBD) with two factors that are proportion of pineapple juice (N0= fruit juice; N1= 1:1, and N2= 1:2) and the concentration of starter (1% v/v and 10% v/v). Each treatment will be repeated 4 times. The main parameter that will be analyzed is the antibacterial activity against Staphylococcus aureus ATCC 25923 with diffusion wells method. The parameters that will be analyzed as a supporting data are pH, total acid, total yeast and BAL. Obtained data will be analyzed statistically by ANOVA (Analysis of Variance) at α = 5 % and continued with DMRT (Duncan's Multiple Range Test) to determine which level of treatment that gives significant differences. The proportion of pineapple juice had significant effect on the antibacterial activity of pineapple kefir, beside starter concentration had not significant effect on the antibacterial activity of pineapple kefir and the interactions between the two factors had not significant effect on the antibacterial activity of pineapple kefir with bacterial test Staphylococcus aureus ATCC 25923.
ISOLASI DAN KARAKTERISASI YEAST AMILOLITIK DARI RAGI TAPE Nurhartadi, Edhi; Rahayu, Endang Sutriswati
Jurnal Teknologi Hasil Pertanian Vol 4, No 1 (2011)
Publisher : Universitas Sebelas Maret (UNS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (269.857 KB) | DOI: 10.20961/jthp.v0i0.13600

Abstract

The demand of sugar increases every year caused by people growth. Indonesia has starchy materialslike tapioca and for long time used to produce food by traditional fermentation with ragi tape. Ragi tapecontains many amylolytic and fermentative microbial. The object of this study was to isolate amylolytic yeastfrom ragi tape, which could be used to produce amylolytic enzymes as an alternative source of amylolyticenzyme from microbial origin. Isolation was done by dilution and spread plate methods on peptone glucoseyeast extract soluble starch (PGYS) agar medium, incubated 30C, for 2 days and purified with same medium.The isolates were then identified based on morphology and physiology profiles. Selection of the strongest isolatewith high amylolytic potent was done with amylolytic activity. The chosen isolate was then used to produceamylolytic enzyme in yeast extract malt extract soluble starch (YMS) medium with variation of soluble starchcontent. 15 amylolytic yeast isolates have been isolated, and then identified with taxonomic keys were suspectedas Debaryomyces vanriji (4 isolates), Saccharomycopsis fibuligera (3 isolates), and Pichia burtonii (8 isolates).They could grow well on starch medium. The result of selection based on amylolytic activity showed that S.fibuligera E-3 was the strongest among the others. From the fermentation, this research obtained the cellscontent increased or obtained logarithmic phase in 1st day of fermentation, and after 2nd day reached stationaryphase medium. The reduction sugar content increased in 1st day of fermentation, after 2nd day was increasedslowly. The soluble starch content was decreased rapidly in 1st day, after 2nd day was decreased slowly. Theglucoamylase activity was high at 1st day, but in 2nd day was low. S. fibuligera had glucoamylase activity 44.13mg glucose/L/min/1 ml enzyme solution.
Karakterisasi Bakteri Asam Laktat Proteolitik pada Bekasam Wikandari, Prima Retno; Suparmo, Suparmo; Marsono, Yustinus; Rahayu, Endang Sutriswati
Jurnal Natur Indonesia Vol 14, No 1 (2012)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (194.903 KB) | DOI: 10.31258/jnat.14.1.120-125

Abstract

Bekasam is a traditional fermented fish from Indonesia. It is made from fish  in which  the gills and  gut  had been  discarded,  washed,  and  mixed with salt, after two days, it was covered  with  rice  and  salt,  and then  fermented  for  5-7 days  at room  temperature.  Bekasam  is perceived to have antihypertensive activity, which is though to be the activity of bioactive peptides, the product of protein degradation  during  the bekasam fermentation. The research was aim to isolate the indigenous lactic  acid  bacteria  that  produce strong  proteolytic  capabilities.  The lactic  acid bacteria were collected from 3  kinds of bekasam (tilapia, milkfish, and tuna bekasam). Isolation  of the lactic acid bacteria was done         on agar medium containing MRS + CaCO3, and  the screening of the proteolytic bacteria  was done by growing the isolated  culture on  skim agar.   The colonies  that produce clear zone  were identified  as proteolytic  lactic acid  bacteria. Some of 180  isolated strains of acid  producing bacteria   that produce clear zone on MRS agar were found 150 strains of  lactic acid  bacteria, and  84  strains  showed  their  proteolytic activity.  The later  were identified morphologically and biochemical as Lactobacillus, Pediococcus and Leuconostoc genera.  Selection  was further conducted based on the height of the proteolytic and homofermentative fermentation activities. Upon species identification using API CH 50 kit,  the  selected strains belong to species of Lactobacillus plantarum, Lactobacillus pentosus, and Pediococcus pentosaseus.
Effect of Inoculation Time for Indigenous Probiotic Bacteria Lactobacillus plantarum DAD-13 on the Physiochemical and Organoleptic Properties of Yoghurt Drink: Pengaruh Waktu Inokulasi Bakteri Probiotik Indigenous Lactobacillus plantarum DAD-13 terhadap Sifat Fisikokimia dan Organoleptik Yoghurt Drink Komalasari, Husnita; Karni, Ine; Heldiyanti, Rina; Arianto, Ahmad Rudi; Rahayu, Endang Sutriswati
JITIPARI (Jurnal Ilmiah Teknologi dan Industri Pangan UNISRI) Vol. 9 No. 1 (2024): JITIPARI
Publisher : Universitas Slamet Riyadi Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33061/jitipari.v9i1.10121

Abstract

Yoghurt drink is a product obtained from fermentation of milk by certain lactic acid bacteria. The aim of this research was to determine how the inoculation time of the indigenous probiotic bacteria Lactobacillus plantarum Dad-13 influences the physicochemical and organoleptic properties of functional plain yoghurt probiotic drink products. This research used an experimental method with a completely randomized design with a single factor, 4 treatments and 3 replications. Reaserch data were analyzed using diversity analysis and Duncan's multiple distance test at a significance level of 5% using SPSS and Minitab 17 software. The results showed that the use of probiotic bacteria significantly affected on viscosity, color L*, color a*, color b*,  °HUE, pH, total dissolved solid, and fat content, but did not significantly influenced on protein content. The use of probiotics as a starter in yoghurt drinks increased viscosity, color brightness, pH and fat content, and have a lower total dissolved solid. The best treatment in this study was joint-inoculation who had viscosity 290,67 mPa.s; color L* 89,46; color a* 2,92; color b* 1,61; °HUE 29,47; pH 5,00; total dissolved solid 16,67 °brix; fat content 2,34 %; and protein content 4,12%. For the scoring test, this treatment has a non-sour taste; the aroma is slightly sour; Slightly thick texture and homogeneous consistency. Meanwhile, the hedonic test has a neutral taste, aroma and texture as well as a color, appearance and consistency were quite liked by the panelists.
PENGARUH PENAMBAHAN BUBUK Lactobacillus plantarum Dad-13 TERHADAP TOTAL BAKTERI ASAM LAKTAT, SIFAT FISIK, DAN SENSORIS SELAI COKELAT KACANG Kamil, Rafli; Dewi, Swastika; Legowo, Anang Mohammad; Mukti, Naura Poetry Nadima; Rahayu, Endang Sutriswati
Jurnal Pangan dan Agroindustri Vol. 12 No. 4: Oktober 2024
Publisher : Department of Food Science and Biotechnology, Faculty of Agriculture Technology, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jpa.2024.012.04.3

Abstract

Lactobacillus plantarum Dad-13 termasuk probiotik yang diisolasi dari dadih yang dapat bermanfaat bagi saluran pencernaan. Penelitian ini bertujuan untuk mengetahui pengaruh penambahan bubuk probiotik L. plantarum Dad-13 terhadap total bakteri asam laktat, warna, total padatan, konsistensi dan karakteristik sensoris serta mengetahui formulasi yang terbaik dalam pembuatan selai cokelat kacang. Model penelitian yang diterapkan yakni Rancangan Acak Lengkap dengan penambahan bubuk L. plantarum Dad-13 yaitu P1 = 1,50%, P2 = 3%, P3 = 4,50%, dan P4 = 6%. Hasil penelitian menujukkan jika penambahan bubuk probiotik L. plantarum Dad-13 dapat meningkatkan nilai total BAL, konsistensi dan menurunkan tingkat kesukaan dari segi rasa, tekstur dan daya oles. Kesimpulan dari penelitian ini adalah bahwa penambahan bubuk probiotik L. plantarum Dad-13 sebesar 3% merupakan perlakuan terbaik, karena telah memenuhi persyaratan minimal konsumsi probiotik serta mendapatkan peringkat tertinggi dalam penerimaan karakteristik sensoris secara keseluruhan.
Hilirisasi Teknologi Sistem Integrasi Tanaman Ternak Ikan (SITTI) Menggunakan Pendekatan Konsep Bio economy, Green Economy, Circular Economy (BGC Economy) Hapsari, Umi; Nihayah, Badiatun; Sutiarso, Lilik; Rahayu, Endang Sutriswati; Purwadi, Didik; Saputra, Wawan
Inovasi Jurnal Pengabdian Masyarakat Vol 2 No 2 (2024): IJPM - Agustus 2024
Publisher : CV Firmos

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54082/ijpm.538

Abstract

Sistem Integrasi Tanaman Ternak Ikan (SITTI) merupakan bentuk integrasi yang saling berkaitan dan memberikan respon positif berupa aliran material dan energi. Teknologi SITTI dinilai mampu menyelesaikan permasalahan yang dihadapi masyarakat di Desa Sriharjo apabila disinergikan dengan konsep BGC Economy yaitu bio economy, green economy dan circular economy. Tujuan pemberdayaan masyarakat melalui penerapan SITTI adalah meningkatkan produktivitas hasil, memberikan nilai tambah pada aspek ekonomi, sosial, dan meningkatkan daya saing produknya, serta efisiensi penggunaan sumberdaya alam dengan meminimalkan limbah dari proses produksi (zero waste) sehingga menghasilkan lingkungan yang lestari dan berkelanjutan. Kegiatan ini dilaksanakan di Desa Sriharjo, Kecamatan Imogiri, Kabupaten Bantul, Yogyakarta dengan sasaran ditujukan kepada kelompok karang taruna tani dan kelompok wanita tani(KWT). Penerapan teknologi SITTI yang terdiri dari subsistem tanaman hortikultura, ternak ayam, budidaya ikan dan budidaya Black Slodier Fly mampu i) meningkatkan produktivitas hasil (prinsip bioeconomy & circular economy) yaitu adanya hasil dari budidaya ayam petelur, ikan lele, tanaman hortikultura dan maggot, ii) memberikan nilai tambah pada aspek ekonomi sosial, dan meningkatkan daya saing produk, setelah dilakukan perhitungan diperoleh nilai BC ratio sebesar 1,73, iii) efisiensi penggunaan sumberdaya alam dengan meminimalkan limbah dari proses produksi (zero waste) dengan memanfaatkan larva maggot BFS untuk mengurai limbah organik menjadi pupuk (prinsip green economy).
Recovery and Molecular Detection of Lactobacillus plantarum Dad-13 from The Feces of Healthy Indonesian Volunteers After Intake of Fermented Milk Hastuti, Sri; Rahayu, Endang Sutriswati; Mariyatun, Mariyatun; Nurfiani, Sri; Widada, Jaka
Indonesian Food and Nutrition Progress Vol 22, No 1 (2025)
Publisher : Indonesian Association of Food Technologists

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ifnp.83841

Abstract

Lactobacillus plantarum Dad-13 is a promising probiotic candidate that can be developed as a starter culture in the production of fermented milk and is commonly isolated from dadih. However, for the bacteria to be considered a probiotic strain, they must survive and thrive in the human digestive tract. This study focused on investigating the recovery level of L. plantarum Dad-13 from fecal samples from healthy volunteers following the consumption of fermented milk. Healthy Indonesian adults (n = 30) were instructed to consume fermented milk containing L. plantarum Dad-13 for 20 days. For a duration of 20 days, 30 healthy Indonesian adults were instructed to consume fermented milk containing L. plantarum Dad-13. Fecal samples were collected at four distinct time intervals: 10 days before ingestion (F1); on day 10 and day 20 while consuming (F2 and F3, respectively); and 10 days after consumption (F4). The viable count of L. plantarum was evaluated by dilution and plating on Lactobacillus plantarum Selective Medium (LPSM). The molecular typing approach utilized repetitive sequence polymerase chain reaction (REP PCR) with the primer BOX A1R. The results indicated that consuming fermented milk led to an increase in the number of lactobacilli and lactic acid bacteria. A total of fifteen (15) isolates of lactic acid bacteria were selected from F1, F2, and F4 periods. The samples obtained from the stool of healthy adults during consumption showed over 99% similarity to L. plantarum Dad-13. Thus, the results showed that L. plantarum Dad-13, as a probiotic candidate, survives in the gut and can be recovered in fecal material. 
Pulp Reduction and Addition of Indigenous Microorganisms as Starter: Effects on Fermented Cocoa Bean Characteristics Fajariyah, Anna; Rahayu, Endang Sutriswati; Farianti Djaafar, Titiek; Utami, Tyas; Anggraini Suroto, Dian; Marwati, Tri
agriTECH Vol 45, No 3 (2025)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/agritech.86796

Abstract

Indonesian cocoa smallholder plantations frequently produced low-quality dry beans due to poor postharvest handling, necessitating improvements through controlled fermentation. This study evaluated pulp reduction and local microorganism starter addition in farmer-level cocoa fermentation on the microbiological, physical, and chemical properties of cocoa beans. Using a Factorial Randomized Block Design, the research included four experimental units: 1) spontaneous fermentation, 2) reduced-pulp fermentation, 3) starteradded fermentation, and 4) reduced-pulp and starter-added fermentation. Pulp was reduced by approximately 35%, and microorganisms utilized were Candida famata HY-37, Lactiplantibacillus plantarum subsp. plantarum HL-15, and Acetobacter sp. HA-37. The five-day fermentation resulted in the growth of yeast, lactic acid bacteria (LAB), and acetic acid bacteria (AAB). This was indicated by a peak fermentation temperature of 45 °C, a decrease in fermentation pH to 3.7, and a decrease in bean pH to 4.7, in line with total titratable acidity increasing. Over the course of two days, the Fermentation Index aboved 1.0. Pulp reduction raised fermentation temperatures and resulted in an earlier peak by the third day. The use of a starter can suppress fungal growth during fermentation. The total fungal population is lower in combining pulp reduction with starter addition fermentation cocoa beans. The physical properties met the Indonesian National Standard (SNI) 2323:2008/Amd I:2010. The chemical characteristics showed that the Fermentation Index was above 1.0, the bean pH values were from 5.16 to 5.36, and the reducing sugar contents were from 1.50 to 1.69 %. Incorporating starter treatment effectively inhibited fungal growth during fermentation. The combination of reducing pulp and adding starter inhibited fungal growth in fermented cocoa beans without affecting their physical and chemical properties.
Co-Authors Anang M Legowo Arianto, Ahmad Rudi Aristya, Aristya Dewi Mayasari Dian Anggraini Suroto Didik Purwadi Edhi Nurhartadi Fajariyah, Anna Farianti Djaafar, Titiek Fibri, Dwi Larasatie Nur Hapsari, Umi Hasan, Pratama Nur Heldiyanti, Rina Helen Joan Lawalata Husnita Komalasari Ine Karni JAKA WIDADA Kamil, Rafli LANGKAH SEMBIRING Lilik Sutiarso Manurung, Nancy Eka Putri Mariyatun Mariyatun, Mariyatun Mohammad Juffrie, Mohammad Muhammad Nur Cahyanto Mukti, Naura Poetry Nadima Nihayah, Badiatun Nugerahani, Ira Nurfiani, Sri Prima Retno Wikandari Rini Yanti, Rini Saputra, Wahyu Dwi Sri Hastuti Suparmo Suparmo, Suparmo Swastika Dewi Tanuwijaya, Shania Angeline Tri Marwati Tyas Utami Wattilete, Jeirel Wawan Saputra Yunan K. Sya’di, Yunan Yustinus Marsono