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All Journal Jurnal Ilmu Pertanian Indonesia Jurnal Hortikultura Indonesia (JHI) METAMORFOSA Journal of Biological Sciences Jurnal Biologi Udayana Bioma : Berkala Ilmiah Biologi JURNAL ANATOMI FISIOLOGI SAINS DAN MATEMATIKA AL KAUNIYAH Biology, Medicine, & Natural Product Chemistry Planta Tropika Majalah Obat Tradisional Kultivasi Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Biodjati Biosaintifika: Journal of Biology & Biology Education Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) Jurnal Biologi Tropika NICHE Journal of Tropical Biology Buletin Anatomi dan Fisiologi (Bulletin of Anatomy and Physiology) Eksakta : Berkala Ilmiah Bidang MIPA Jurnal Riset Biologi dan Aplikasinya Life Science Makara Journal of Science Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek) Jurnal Bioteknologi & Biosains Indonesia (JBBI)
Yulita Nurchayati
Departemen Biologi Fakultas Sains Dan Matematika Universitas Diponegoro, Semarang
Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Earth & Planetary Sciences Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Public Health

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Journal : Jurnal Bioteknologi

 1 
RESPONSE OF SEED GERMINATION AND GROWTH OF Nepenthes gymnamphora Nees IN VITRO TO THE CONCENTRATION OF MS MINERAL SALT, PEPTONE AND THIDIAZURON Yulita Nurchayati
Jurnal Bioteknologi dan Biosains Indonesia Vol. 9 No. 1 (2022)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

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Abstract

Nepenthes gymnamphora Nees is a Java's rare endemic species. Ex-situ conservation of this endangered species can be done through in vitro culture technique. The aims of this study were to determine (1) the mineral salt concentration of MS basal media and addition of peptone (P) on N. gymnamphora seed germination and seedling emergence and (2) the effects of TDZ in ½MS medium on seedling growth. Seeds were surface sterilized and cultured on four media formulations (½MS, MS, ½MS+P, MS+P) for 8 weeks. In the second experiment, ten-week-old seedlings, 0,25 cm in length were cultured on ½MS supplemented with 0, 0,5, 1,0, or 1,5 mg/L TDZ. Seedling growth was recorded at 8 weeks of culture. Results of this experiment showed that ½MS was the best medium for N. gymnamphora seed germination as indicated by the highest percentage of germination, the tallest seedling, and the fastest seedling emergence. Moreover, the best growth of N. gymnamphora was found on ½MS without TDZ.
THE EFFECT OF LONG SOAKING OF SEEDS IN KNO3 SOLUTION AND VARIATIONS IN PLANTING MEDIA ON THE GERMINATION OF CIPLUKAN (Physalis angulata L.) SEEDS IN VITRO hartati, Puji; Hastuti, Endah Dwi; Setiari, Nintya; Nurchayati, Yulita
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 11 No. 1 (2024)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2024.4968

Abstract

The Physalis angulata L. (ciplukan), originating from the Solanaceae family, contains secondary metabolites that can be used as medicinal materials. The provision of Physalis angulata seeds is hindered by seed dormancy. Dormancy can be broken by employing a 0.2% w/v KNO3 solution with a specific soaking period. This study aims to optimise the duration of the soaking period for Physalis angulata seed germination and the in vitro growing medium. The seeds were soaked in a 0.2% w/v KNO3 solution for 6 hours, 12 hours, and 24 hours. The soaked seeds were planted in sterile tissue media moistened with Murashige and Skoog (MS) medium solution, a 0.2% w/v KNO3 solution, and distilled water. Generally, the dormancy of Physalis angulata seeds can be broken by soaking them in a 0.2% w/v KNO3 solution. The results of this study indicated that Physalis angulata seeds soaked in a 0.2% w/v KNO3 solution for 6 hours and 12 hours, then planted in sterile tissue media moistened with the MS medium solution, exhibited an optimal germination response.
Co-Authors Abdul Khafid Abdul Khafid, Abdul Agustin Noviati Aini, Nabilah Anggi Krisdianto Ari Indrianto Ari Indrianto Diah Nurmaningrum Ditasya Putri, Novika Endah Dwi Hastuti Endang Saptiningsih Endang Saptiningsih Erma Prihastanti Erma Prihastanti Fakhri Fadhlurrohman Pratama Fathiyah Afiah R Fathiyah Afiah R, Fathiyah Fella Suffah Meinaswati Fella Suffah Meinaswati Fetryani Soni Manurung Fitrian Agna Mahayaning, Fitrian Agna Fiva Andriyani Hana, Puji Nur Hartati, Puji I’anatushshoimah, I’anatushshoimah Ika Susanti Hendriyani Indriani, Reni Istiqomah, Azura Muzdalifah Jefri Saputro Karend, Azzah Hanun Abas L. Hartanto Nugroho Latif, Fahmi Nur Aida Laurentius H Nugroho Luaeliyah, Masrukhatul Madha Kurniawan Misrofah, Sofatu Misrofah, Sofatun Mochammad Syaifuddin Munifatul Izzati NIDA, KHOIRIN Nintya Setiari Nintya Setiari Nita Kumalasari Dewi Nur Cahyani, Evi Prameswari, Ratna Apriliani Puji Nur Hana Putra, Agita Christyaji Putri Anugrah Maulidya Rashid, Fatimah Azzahra Ahmad Reni Indriani Rina Budi Astuti Rini Budi Hastuti Rini Budihastuti Rini Budihastuti Rizqi Fadlia Julianti Sadiah, Fitriatus Safinah, Safinah Santosa Santosa Santosa Santosa Santosa, Faradita Azzahra Setiana, Devi Vira Sri Darmanti Sri Haryanti Sri Haryanti Sri Widodo Agung Suedy Sulistyowati Sulistyowati Surya Kurnia Hayati Titis Yuliana Ulva, Maria Utami, Ika Nur Wahyu Hadayani Wiraputra, Muhammad Dwijunianto