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The Effect of Human Platelet-Rich Plasma and L-Ascorbic Acid on Morphology, Proliferation, and Chondrogenesis Ability towards Human Adipose-Derived Stem Cells Imam Rosadi; Karina Karina; Iis Rosliana; Siti Sobariah; Irsyah Afini; Tias Widyastuti; Anggraini Barlian
Molecular and Cellular Biomedical Sciences Vol 3, No 1 (2019)
Publisher : Cell and BioPharmaceutical Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (5637.848 KB) | DOI: 10.21705/mcbs.v3i1.43

Abstract

Background: Microtia is a congenital malformation in the external ear due to cartilage defect. Adipose-derived stem cells (ADSC) is promising cells to develop cartilage tissue engineering for microtia. In this study, we focused on proliferation and chondrogenesis of ADSC in three different media, which consist of 10% fetal bovine serum (FBS), 10% FBS with L-ascorbic acid, and 10% human platelet rich plasma (PRP). Methods: ADSC were induced to differentiate into adipocytes, chondrocyte and osteocytes. ADSC morphology, proliferation and population doubling time was compared in three different media and analysed. Observation and alcian blue staining were done every 7 days to assess chondrogenic potency of ADSC from each treatment.Results: Isolated ADSC were able to differentiate into adipocytes, osteocytes and chondrocytes. ADSC in all group have fibroblast-like morphology, but cells in 10% FBS and 10% FBS with LAA group were flattened and larger. ADSC in 10% PRP group proliferates faster than 10% FBS with and without LAA. PDT values of ADSC were 34 hours, 44 hours and 48 hours, respectively for 10% PRP, 10% FBS with LAA and 10% FBS group. Alcian blue staining revealed that ADSC in 10% FBS with LAA and 10% PRP were able to proceed to chondrogenesis when cultured time were prolong up to 21 days, but not with ADSC in 10% FBS. Conclusion: We conclude that adding 10% FBS with LAA or 10% PRP into medium culture can support proliferation and chondrogenesis of ADSC. Key words: human ADSC, PRP, L-ascorbic acid, proliferation, chondrogenesis
Analisis Tingkat Keparahan Penyakit pada Daun Tanaman Pangan dengan Menggunakan Software ImageJ dan Plantix Imam Rosadi; Cici Lis Qurrotun Ayuni; Indah Nurcahyani; Muhammadiyah Muhammadiyah; Irene Putri Priscilla Butar-Butar; Linda Oktavianingsih
Bioscientist : Jurnal Ilmiah Biologi Vol 10, No 1 (2022): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v10i1.4575

Abstract

Plant disease is the physiological process that causes them to become abnormal. Plant diseases attack vegetative organs in the form of roots, stems, and leaves or generative organs of these plants. Plants become sick due to fungi, bacteria, or viruses that cause many symptoms of disease in plants, such as yellow spots, infection, and nitrogen deficiency (N). The purpose of this case study was to determine the severity of disease on gourds leaves (Lagenaria siceraria), bitter melon leaves (Momordica charantia), cassava leaves (Manihot esculenta), purple eggplant leaves (Solanum melongena), and terung pipit leaves (Solanum torvum) by using the Plantix software and ImageJ software methode. Based on the results of this case study, it was found that the severity of disease using ImageJ software on gourds leaves (4.56%), bitter melon leaves (1.93%), cassava leaves (8.49%), purple eggplant leaves (49.45%) and terung pipit leaves (14.88%) which are generally food crops are caused by viruses and fungiand based on the results of the Plantix software data, the causes of gourds leaves, bitter melon leaves, cassava leaves, purple eggplant leaves and terung pipit leaves respectively are caused by downy mildew, fungus, whitefly, leaf spot algae and leafhoppers.
Pengaruh Penambahan Berbagai Konsentrasi L-Ascorbic Acid Terhadap Kemampuan Migrasi Adipose-Derived Stem Cells Asal Manusia Karina Karina; Imam Rosadi; Wismo Reja Subroto; Alfida Zakiyah; Irsyah Afini; Iis Rosliana; Tias Widyastuti; Siti Sobariah
JURNAL Al-AZHAR INDONESIA SERI SAINS DAN TEKNOLOGI Vol 5, No 4 (2020)
Publisher : Universitas Al Azhar Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36722/sst.v5i4.404

Abstract

Abstrak - Mesenchymal Stem Cells (MSCs) merupakan sel yang memiliki kemampuan memperbarui diri dan berdiferensiasi menjadi berbagai jenis sel. MSCs berperan penting dalam perbaikan dan regenerasi jaringan. Adipose-Derived Stem Cells (ADSCs) merupakan MSCs yang didapatkan dari jaringan lemak. ADSCs telah banyak digunakan dalam studi MSCs karena ketersediaan sumber sel yang banyak dalam jaringan tubuh dan proses isolasinya lebih mudah serta memiliki risiko yang lebih rendah. Pada studi ini, ADSCs digunakan untuk mengetahui kemampuan migrasi dalam medium yang mengandung fetal bovine serum 10% (FBS) dengan penambahan L-Ascorbic Acid (LAA) konsentrasi 0%, 1%, 3% dan 6%. Kuantifikasi kemampuan migrasi dilakukan dengan menggunakan teknik goresan luka. ADSCs yang dikulturkan menunjukkan morfologi seperti fibroblas. Luas penutupan luka menunjukkan hasil tertinggi dengan perbedaan yang tidak signifikan pada penambahan konsentrasi LAA 3% dan 6% dibandingkan penambahan LAA 0%, 1% dan kontrol. Hasil ini juga ditunjukkan pada pasase 7, 8, dan 9. ADSCs menunjukkan kemampuan migrasi yang optimal pada penambahan LAA 3% dan 6% dibandingkan medium dengan penambahan LAA 0%, 1% dan kontrol.Abstract - Mesenchymal stem cells (MSCs) are multilineage cells with the ability to self-renew and differentiate into a variety of cell types. MSCs play important role in tissue repair and regeneration. Adipose-Derived Stem Cells (ADSCs) are MSCs derived from fat tissue. ADSCs has been widely used in MSCs studies because it require more cell sources in body tissue and the process of isolation is easier and has lower risk. In this study, ADSCs were used to determine migration ability in a medium containing 10% bovine fetal serum (FBS) using 0%, 1%, 3% and 6% of L-Ascorbic Acid (LAA) concentrations. Quantification of migration ability was done by using scratch technique. ADSCs were cultured display fibroblasts-like morphology. The closure of wound area is highest with not significantly difference in LAA concentrations of 3% and 6% compared to LAA 0%, 1% and control. This results were also showed on 7, 8, and 9 cell passage. ADSCs showed optimal migration ability at LAA 3% and 6% compared to media with LAA 0%, 1% and control.Keywords - ADSCs, Cells Migration, LAA.
Profil Mesenchymal Stem Cell (MSC) Pasien Klinik Hayandra Pada Media Kultur Bersuplemen Menggunakan Flow Cytometry Adlia Khalisha; Riris Lindiawati Puspitasari; Karina F Moegni; Imam Rosadi; Iis Rosliana
JURNAL Al-AZHAR INDONESIA SERI SAINS DAN TEKNOLOGI Vol 4, No 4 (2018)
Publisher : Universitas Al Azhar Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36722/sst.v4i4.310

Abstract

Abstrak - Mesenchymal Stem Cell (MSC) berpotensi untuk digunakan dalam pengobatan karena bersifat multipoten, dapat bermigrasi, dapat berproliferasi. MSC dikultur menggunakan media basal dengan penambahan suplemen, seperti Fetal Bovine Serum (FBS) dan Platelet-Rich Plasma(PRP) untuk mendukung pertumbuhannya. FBS dan PRP memiliki bahan dasar yang berbeda yaitu FBS berasal dari serum fetus sapi sedangkan PRP berasal dari plasma manusia, sehingga perlu dilakukan analisis karakter MSC yang dikultur pada media dengan penambahan FBS atau PRP. Tujuan penelitian adalah untuk menganalisis profil MSC yang dikultur pada media dengan penambahan FBS 10% atau PRP 10%. Metode yang dilakukan adalah isolasi MSC, pewarnaan MSC, penentuan populasi sel, dan analisis statistik. Penanda yang digunakan berupa CD73, CD90, CD105 dan Lineage negative (CD45/CD34/CD11b/CD19/HLA-DR). Sampel MSC didapatkan dari 8 orang pasien Klinik Hayandra. Hasil dari penelitian menunjukkan bahwa tidak ada perbedaan yang signifikan dari seluruh marker CD, yaitu Lin-(-)/CD90+ (p = 0,16), Lin-(+)/CD90+ (p = 0,43), dan CD105+/CD73+ (p = 0.5). Walaupun tidak memiliki perbedaan nyata, namun PRP cenderung memberikan hasil yang lebih baik daripada FBS, sehingga PRP dapat dijadikan sebagai suplemen alternatif dalam mengkultur MSC.Abstract - Mesenchymal Stem Cell (MSC) were used in many therapy in curing degenerative diseases. Because of its multipotent character, MSC could migrated, proliferated, and differentiated. In culture, MSC needed some suplements such as Fetal Bovine Serum (FBS) and Platelet-Rich Plasma (PRP) to support the growth. FBS and PRP had different ingredients based on their resources. FBS is derived from bovine fetus serum whereas PRP comes from human plasma, so became necessary to analyze MSC characters cultured in media with FBS or PRP suplements. The purpose of research was to analyze the MSC profile cultured with addition of 10% FBS and 10% PRP. The methodology were MSC isolation, MSC staining, cell population determination, and statistic analysis. The markers used are CD73, CD90, CD105 and Lineage negative (CD45 / CD34 / CD11b / CD19 / HLA-DR). Samples were obtained from 8 patients of Hayandra Clinic. The results of research showed that there were no significantly differences from all CD markers. Lin - (-) / CD90 + (p = 0.16), Lin - (+) / CD90 + (p = 0.43), and CD105 + / CD73 + (p = 0.5) gave different character in FBS or PRP suplementation. Although no significant difference, PRP tends to give better results than FBS in culturing MSC. This finding informed that PRP could be used as an alternative suplement in culturing MSC.Keywords - Mesenchymal Stem Cell (MSC), Fetal Bovine Serum (FBS), Platelet- Rich Plasma (PRP)
Profil Peripheral Blood Mononuclear Cells (PBMC) Pasien dengan Berbagai Usia Menggunakan Flow Cytometry di Klinik Hayandra Masayu Nadhira; Riris Lindiawati Puspitasari; Karina F Moegni; Imam Rosadi; Iis Rosliana
JURNAL Al-AZHAR INDONESIA SERI SAINS DAN TEKNOLOGI Vol 4, No 4 (2018)
Publisher : Universitas Al Azhar Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36722/sst.v4i4.312

Abstract

Abstrak - Profil Peripheral Blood Mononuclear Cells (PBMC) setiap orang dapat berbeda-beda. Salah satu faktor yang diduga mempengaruhi adanya perbedaan tersebut adalah usia. Manusia berusia lanjut lebih mudah terserang penyakit karena terjadinya penurunan fungsi dan jumlah sel imun. Oleh karena itu, perlu dilakukan analisis komposisi PBMC untuk mengetahui profil PBMC seseorang berdasarkan usia, sehingga dapat diketahui jumlah sel imun dalam tubuh manusia. Tujuan dari penelitian ini adalah untuk menganalisis profil PBMC pasien dengan berbagai usia di Klinik Hayandra menggunakan flow cytometry. Manfaat dilakukannya kegiatan penelitian ini adalah diperolehnya informasi mengenai profil PBMC pasien di Klinik Hayandra dan kaitannya dengan usia. Metode yang dilakukan diantaranya adalah isolasi PBMC, proses pewarnaan PBMC, dan penentuan populasi sel menggunakan flow cytometry. Cluster of Differentiation (CD) yang digunakan pada kegiatan penelitian ini diantaranya adalah CD3, CD19, CD8, CD56, dan CD314 atau disebut juga NKG2D (Natural Killer Group 2 member D). Hasil yang diperoleh pada kegiatan penelitian ini adalah ditemukannya korelasi negatif yang bermakna antara persentase jumlah sel limfosit T sitotoksik (CD3+/CD8+) dengan usia dan ditemukannya korelasi positif yang bermakna antara persentase jumlah sel NKT (CD3+/CD56+) dengan usia. Dengan demikian usia pasien dapat mempengaruhi jumlah sel imun pasien.Abstract - Peripheral Blood Mononuclear Cells (PBMC) profiles of each person can different. One of the factors that allegedly affects the difference is age. Elderly human are more susceptible attacked disease due to decreased function and number of immune cells. Therefore, it is necessary to do analyze PBMC composition to know the PBMC profile of person, so that can be known the number of immune cells in the human body. The purpose of this research is to analyze the PBMC profile of patients with various ages in the Hayandra Clinic using flow cytometry. The benefit of doing this practical work is obtained information about the PBMC profile of patients in the Hayandra Clinic and its relation to age. Methods which are conducted among others are PBMC isolation, PBMC staining process, running flow cytometry, and determination of cell population. Cluster of Differentiation (CD) used in this practical work among others are CD3, CD19, CD8, CD56, and CD314 or also called NKG2D (Natural Killer Group 2 member D). The results obtained in this research were the found of significant negative correlation between percentage of cytotoxic T cell lymphocytes numbers (CD3+/CD8+) with age and found a significant positive correlation between percentage of NKT cell numbers (CD3+/CD56+) with age. So it can be concluded that the patient's age can affect the number of the patient immune cells.Keywords - Peripheral Blood Mononuclear Cells (PBMC), flow cytometry, age, Cluster of Differentiation (CD)
Pengabdian Masyarakat: Pemanfaatan Nasi Bekas sebagai Pupuk dan Pestisida Tanaman Rumah di PKK Perumahan Jurang Mangu Indah, Bintaro Nita Noriko; Arief Lelono Arum; Hamisya Nurindriani; Crisnia Crisnia; Cyndi Marcelina; Imam Rosadi; Habib Pangeran; Dwi Atmi Narwati
JURNAL Al-AZHAR INDONESIA SERI SAINS DAN TEKNOLOGI Vol 1, No 4 (2012)
Publisher : Universitas Al Azhar Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36722/sst.v1i4.90

Abstract

Nasi bekas dan sisa makanan berbahan dasar karbohidrat lainnya merupakan jenis sampah organik yang dapat didaur ulang, tetapi biasanya hanya langsung dibuang atau diberikan ke unggas. Berdasarkan penelitian yang telah dilakukan bahan-bahan tersebut dapat dijadikan produk pupuk dan pestisida melalui fermentasi kapang yang disebut kapangisida. Kegiatan pengabdian masyarakat (abdimas) ini bertujuan untuk memperkenalkan pembuatan Kapangisida tersebut. Sasaran kegiatan ini adalah kelompok masyarakat dalam hal ini Pemberdayaan dan Kesejahteraan Keluarga (PKK) yang menaruh perhatian pada perawatan tanaman rumah yaitu PKK Perumahan Jurang Mangu Indah Bintaro. Hasil survei menunjukkan masyarakat di perumahan  tersebut belum melakukan daur ulang nasi bekas menjadi kapangisida. Oleh karena itu,  produk ini merupakan produk baru di masyarakat. Kelompok PKK  ini juga bersedia untuk melakukan duplikasi proses pembuatan produk yang bermanfaat bagi tanaman.AbstractThe used of rice and the waste of foods based on carbohydrate have not been recycled and are usually discarded or given directly to the poultry. Based on research that has been done, the waste can be converted to be fertilizer and pesticide products through the mold fermentation process. The product then called kapangisida. Our public service is aimed at introducing the making process of kapangisida. The target group, in this case is Empowerment and  Family Welfare  (PKK) that has concern at household plant treatment. We have selected PKK Perumahan Jurang Mangu Indah that is situated in Bintaro. The result founds that the PKK has not recycled used rice and carbohydrate-based foods to be kapangisida. Therefore, this product is a new product in the community. The PKK also will do the duplication of kapangisida making process that beneficial to the plant.
TINGKAT KETAATAN MASYARAKAT DALAM MELAKSANAKAN PROTOKOL KESEHATAN SELAMA PANDEMI COVID-19 DI KOTA SAMARINDA KALIMANTAN TIMUR Nova Hariani; Wafif Azizah; An Nissa falaq Qurrahmah; Nur Ulmi; Olivia Yolanda Lawono; Rizka Shofiyya Ramadhani; Imam Rosadi
Jurnal Infokes Vol 12 No 2 (2022): Jurnal Ilmiah Rekam Medis dan Informatika Kesehatan
Publisher : Universitas Duta Bangsa Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Corona virus disease 2019 (COVID-19) is a disease that caused by the Severe Acute Respiratory Syndrome CoronaVirus 2 (SARS-CoV-2). This virus attacks the human respiratory tract and accompanied by initial symptoms such as fever, cough, sore throat, even losing of sense, taste or smell. COVID-19 is still a pandemic in various countries including Indonesia so far. The Indonesian government has implemented various efforts to prevent the transmission of the virus, starting with the implementation of health protocols to maintain personal and environmental hygiene with Large-Scale Social Restrictions since the first positive cases of COVID-19 in 2020. The Samarinda City Government participated in implementing health protocols policies cover Testing, Tracing, and Treatment (3T), Large Scale Social Restrictions, and higher level of social restrictions . This study will research the level of community compliance in implementing Health protocols during the COVID-19 pandemic in Samarinda. This research was conducted through a digital survey using Googleform. The results show that Samarinda citizens has an increase on the application of health protocols as evidenced by the awareness of washing hands and using Hand sanitizer. However, in maintaining distance and self isolation, the community still does not have high awareness enough that has a potential to create new cluster by the opportunities of virus tranmission.
Severity analysis of fruit plant diseases based on leaf symptoms using ImageJ software An Nissa Falaq Qurrahmah; Umi Mutfidasari; Putri Inayah Aisyah; Irene Putri Pricillia Butar-Butar; Imam Rosadi; Linda Oktavianingsih
Bioma : Jurnal Ilmiah Biologi Vol. 11 No. 2: October 2022
Publisher : Universitas PGRI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/bioma.v11i2.10467

Abstract

Diseases in plants are abnormal and detrimental physiological processes plants. Plant organs commonly affected by the disease are leaves, roots, fruit, and stems. Diseases in plants can generally be caused by bacteria, fungi, viruses, and algae that cause diplodia, red rust patches/spots, scabies, and downy mildew. This study aims to determine the severity of disease in fruit plants using ImageJ and plantix. Based on the results of the study, found five types of fruit plants were used, namely grape vines (Vitis vinifera), sapodilla (Manilkara zapota), mango (Mangifera indica), rambutan (Nephelium sp.), and lime plants (Citrus aurantifolia) with different severity of plant diseases. The highest severity of the disease was found in rambutan leaves (43.73%) caused by fungi, and the lowest severity was found in lime leaves (16.12%) caused by fungi.
Perkembangan Penelitian di Bidang Teknologi Membran Menggunakan Pendekatan Cellulose Acetate Mixed Matrix Membrane: Studi Analisis Bibliometrik Panggulu Ahmad Ramdhani Utoro; Imam Rosadi
Jurnal Ilmiah Universitas Batanghari Jambi Vol 22, No 3 (2022): Oktober
Publisher : Universitas Batanghari Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33087/jiubj.v22i3.2705

Abstract

The development of science needs to be done so that research gaps can be traced and known so that sustainable research occurs. In this study, a bibliometric analysis approach was carried out with the keywords "cellulose acetate mixed matrix membrane". The data was obtained from the Scopus portal using Publish or Perish (PoP) software with a search range of 2001 to 2022. The data obtained was then analyzed using the 2019 version of Microsoft Excel and VosViewer. PoP search results show that from 2001 to 2022 there were 137 articles. There is an increase in the number of documents with the year of the search. The highest number of article documents in 2020 was 26 articles. with the number of documents, the trend of the highest number of citations was obtained in the range of 2010 – 2014. Portal Journal of Membrane Science is a journal that reports the most different data related to these keywords as many as 12 articles. The most prolific writer as a correspondent writer is Mubashir, M with a total of 6 articles. The most frequently encountered keywords in article manuscripts were “cellulose acetate”, “mixed matrix membrane”, and “fabrication”. The three keywords form three interrelated clusters.
Peningkatan Ekonomi Rumah Tangga Petani Melalui Sistem Elektronik Rencana Definitif Kebutuhan Kelompok Tani dan Pemanfaatan Lahan Pekarangan Pangan Lestari Alam, Megawati Citra; Aji, Satriya Bayu; Rosadi, Imam; Artini, Widi; Kustiani, Edy; Supandji, Supandji
JATIMAS : Jurnal Pertanian dan Pengabdian Masyarakat Vol. 4 No. 1 (2024): MEI
Publisher : Kadiri University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30737/jatimas.v4i1.5585

Abstract

This community service activity explores ways to improve the economy of farmer households through an electronic system for definitive plans for the needs of farmer groups and the role of women farmer groups through sustainable use of food plots. By coordinating this program, the synergy between agricultural activities is created to achieve broader goals regarding food security and sustainable natural resource management. Challenges such as limited access to subsidized fertilizers encourage the use of E-RDKK, which requires farmer collaboration for effective planning and distribution. Optimizing P2L is an alternative for sustainable food production in the home garden. Reduce dependence on external resources such as subsidized fertilizer and strengthen local food security. This activity was carried out using an open questionnaire before (pre-test) and after (post-test) lecture sessions, discussion sessions, and training sessions. Next, the team continued with a focus group discussion (FGD) session for the follow-up stage of the training. Then, proceed with evaluation using a qualitative approach to thematic analysis techniques and cross-tabulation. The team looked for themes that emerged from the questionnaire response data and recorded the FGD transcripts. Apart from that, data organization was also carried out with the help of the MAXQDA 2020 Application. This activity provides insight into how the RDKK and P2L programs can be an essential tool in improving the economy of farmer households through sustainable food security at the local level, primarily through community-based land management strategies. The policy recommendations and sustainable practices resulting from this activity will support similar efforts in other regions in Indonesia that face the same challenges.Kegiatan pengabdian kepada masyarakat ini mengeksplorasi cara peningkatan ekonomi rumah tangga petani melalui sistem elektronik rencana definitif kebutuhan kelompok tani dan peran kelompok wanita tani melalui pemanfaatan lahan pekarangan pangan lestari secara berkelanjutan. Dengan mengkoordinasikan program ini, tercipta sinergi antar aktivitas pertanian untuk mencapai tujuan yang lebih luas mengenai ketahanan pangan dan pengelolaan sumberdaya alam yang berkelanjutan. Tantangan seperti akses terbatas pada pupuk bersubsidi mendorong penggunaan E-RDKK, yang memerlukan kolaborasi petani untuk perencanaan dan distribusi yang efektif. Pengoptimalisasian P2L menjadi alternatif produksi pangan yang berkelanjutan di pekarangan rumah. Mengurangi ketergantungan terhadap sumberdaya eksternal seperti pupuk bersubsidi dan memperkuat ketahanan pangan lokal. Kegiatan ini dilakukan menggunakan kuesioner terbuka dilakukan sebelum (pra-tes) dan sesudah (pasca-tes) sesi ceramah, sesi diskusi, dan sesi pelatihan. Selanjutnya, tim melanjutkan dengan sesi focus Group Discussions (FGD) untuk tahap tindak lanjut dari pelatihan. Kemudian dilanjutkan dengan evaluasi menggunakan pendekatan kualitatif untuk teknik analisis tematik dan tabulasi silang. Tim, mencari tema yang muncul dari data respons kuesioner dan mencatat transkrip FGD. Selain itu, organisasi data juga dilakukan dengan bantuan Aplikasi MAXQDA 2020. Kegiatan ini memberikan wawasan tentang bagaimana RDKK dan program P2L dapat menjadi alat penting dalam peningkatan ekonomi rumah tangga petani melalui ketahanan pangan yang berkelanjutan di tingkat lokal. Terutama melalui strategi manajemen lahan berbasis komunitas. Rekomendasi kebijakan dan praktik berkelanjutan yang dihasilkan dari kegiatan ini diharapkan akan mendukung upaya-upaya serupa di wilayah lain di indonesia yang menghadapi tantangan yang sama.
Co-Authors Abriyanto, Hery Adlia Khalisha Afini, Irsyah Al Hasbi, Ezra Alam, Megawati Citra Alfida Zakiyah An Nissa falaq Qurrahmah An Nissa Falaq Qurrahmah Anggraini Barlian Anggraini Barlian Arief Lelono Arum Aryani, Retno Cici Lis Qurrotun Ayuni Crisnia Crisnia Cyndi Marcelina DEMO, HILDIANA APRILIANI DHIU Dewi Elfidasari Dwi Atmi Narwati Edy Kustiani Fawaidul Badri Habib Pangeran Hamisya Nurindriani Haroh, Istik Hazmi, Nubli Iis Rosliana Iis Rosliana Iis Rosliana Iis Rosliana Iis Rosliana Iis Rosliana Indah Nurcahyani Iqbal Purnama, Muhammad Irene Putri Pricillia Butar-Butar Irene Putri Priscilla Butar-Butar Irsyah Afini Irsyah Afini Irsyah Afini Irsyah Afini Irsyah Afini Irsyah Afini Karina Karina Karina Karina Karina Karina Karina Komang A Wahyuningsih Komang A Wahyuningsih Komang A. Wahyuningsih Komang A. Wahyuningsih Komang A. Wahyuningsih Linda Oktavianingsih Linda Oktavianingsih Masayu Nadhira Muhammad Fauzi Arif Muhammad Fauzi Arif Muhammadiyah Muhammadiyah Mukh Syaifudin Ngatmari Nita Noriko Nova Hariani Nur Ulmi Olivia Yolanda Lawono Panggulu Ahmad Ramadhani Utoro Pratiwi, Anggita Endar Putranto, Bima Arya Putri Inayah Aisyah Rahayu, Sekar Riris Lindiawati Puspitasari Rizka Shofiyya Ramadhani Rosliana, Iis Rudy Agung Nugroho Satriya Bayu Aji, Satriya Bayu Siti Sobariah Siti Sobariah Siti Sobariah Siti Sobariah Siti Sobariah Siti Sobariah Sobariah, Siti Sugianur, Sugianur Supandji, Supandji Tias Widyastuti Tias Widyastuti Tias Widyastuti Tias Widyastuti Tias Widyastuti Umi Mutfidasari Umilasari, Reni Wafif Azizah Wahyuningsih, Komang A Widi Artini Widyastuti, Tias Wismo Reja Subroto Yuliani, Ferdina Yuliatin, Ervinda