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The Effect Of Neon Light In The Clinical Laboratory On Bilirubin Levels Pramonodjati, F; Jyh Chayang, Pang; Ayu Utami, Nataly
Proceeding of the International Conference Health, Science And Technology (ICOHETECH) 2024: Proceeding of the 5th International Conference Health, Science And Technology (ICOHETECH)
Publisher : LPPM Universitas Duta Bangsa Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47701/icohetech.v5i1.4243

Abstract

The average number of patients served by the Grogol Health Center in the general polyclinic is 40 to 45 patients every day. Based on these data, there is a buildup of 7-10 patients, with an average service time of 10 minutes per patient. From the results of further observations, there is a buildup of patients in the general polyclinic of the Grogol Health Center. This study aims to solve the queue problem with the line balancing method and develop an online registration application at the Grogol Health Center that can increase the efficiency of the patient registration process and reduce waiting time. Line Balancing is a method for solving queue system problems. So that the results of this study are the creation of an integrated application and online registration that can reduce waiting time
Activity of Enzyme in Escherichia Coli Through Molecular Techniques Liss Dyah Dewi Arini; Fredericus Pramonodjati
International Journal Of Health Science Vol. 5 No. 2 (2025): International Journal of Health
Publisher : Lembaga Pengembangan Kinerja Dosen

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55606/ijhs.v5i2.4991

Abstract

Enzymes are protein biomolecules that act as catalysts in various biochemical reactions, both in biological systems and industrial applications. The uniqueness of enzymes lies in their ability to accelerate the rate of reactions with a high degree of specificity towards certain substrates without undergoing permanent changes in their chemical structure. Enzyme activity is strongly influenced by various environmental factors, such as temperature, pH, substrate concentration, and the presence of inhibitors or activators. Therefore, quantitative testing of enzyme activity is an important step in understanding the characteristics of enzymes and their applications in various fields. Escherichia coli produces Extended-Spectrum Β-Lactamase Enzyme (ESBL) and plays a role in damaging the structure of beta lactam antibiotics so that the antibiotics cannot kill bacteria. Bacteria that produce ESBLs need to be watched out for because ESBLs are produced by genes located on plasmids, which can easily be transferred to other bacteria, and often also carry resistance genes to other antibiotics. Objective: to accurately measure the activity or concentration of enzymes in samples of Escherichia coli bacteria and understand the influence of variables such as substrate concentration on the reaction rate. Method: spectrophotometry through enzyme extraction, making a standard curve and testing enzyme activity against variations in substrate concentration. Results: samples with concentrations of 0.1 and 0.3 showed good and appropriate absorbance results. However, in the sample 0.5 ; 0.7 ; and 1.0 indicates an absorbance number that is slightly higher than it should be. Conclusion: the enzyme in Escherichia coli bacteria has good activity at sample concentrations of 0.1 and 0.3.
Inovasi Model Pemanasan Secara Cerdas dalam Mendukung Terciptanya Jantung Sehat pada Posyandu Lansia Fredericus Pramonodjati; Liss Dyah Dewi Arini; Anggraini Sih Prabandari; Nataly Ayu Utami; Selvina Dwi Rahmawati; Azizah Zahra Nur Fitria; Azzahra Nabilla Atha; David Valentino Iskandar; Erika Dwi Susanti; Nur Aini Fatna Fadilla
Jurnal Pengabdian Ilmu Kesehatan Vol. 5 No. 3 (2025): November: Jurnal Pengabdian Ilmu Kesehatan
Publisher : Lembaga Pengembangan Kinerja Dosen

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55606/jpikes.v5i3.5991

Abstract

The “Sejahtera” Elderly Posyandu is one of the integrated health posts (Posyandu) fostered by the Jaten II Ngringo Community Health Center (UPT) in Jaten, Karanganyar. Ngringo is developing the “Ngudi Sehat” Quality Family Village, which focuses on improving the quality of life of families. In terms of health potential, the presence of the Mojosongo 2 Special Surgery Hospital, the Hajah Rodiah Maternity Hospital, and the Jaten 2 Community Health Center supports health services for the community. Ngringo has a strategic location, as the gateway to Karanganyar Regency, Ngringo has the potential to be a center for trade, distribution, and public services, and has supporting infrastructure such as markets, terminals, and flyovers to support economic activities and mobility. This community service model (method) uses 7 (seven) main activity stages, namely Identification of partner problems and needs, preparation of innovative materials and media, apperception, implementation of the Warming Model (counseling and training to posyandu cadres and the elderly, cooking healthy food for cadres and participants, simple health checks for cadres and participants, provision of simple health equipment), monitoring, evaluation and mentoring, documentation and reporting and termination. The results of this activity are The conclusion of the results of this PMP activity is. The output of this PMP is a PKM journal article indexed by Sinta 4 https://journalshub.org/index.php/JPIKes, Jawa Pos newspaper, activity videos and books.
Identifikasi Karakteristik Mikroskopik dan Makroskopik pada Jamur Rhizopus sp., Aspergillus sp., dan Penicillium sp., pada Beberapa Sampel Makanan Liss Dyah Dewi Arini; Fredericus Pramonodjati; Margaretha Alesandria Natasya Weka
USADA NUSANTARA : Jurnal Kesehatan Tradisional Vol. 4 No. 1 (2026): USADA NUSANTARA
Publisher : Institut Nalanda

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47861/usd.v4i1.2150

Abstract

Fungi are microorganisms often found in food and can act as spoilage agents or have certain benefits. Rhizopus sp., Aspergillus sp., and Penicillium sp. are types of fungi commonly found in various foods with different characteristics. This study aims to determine the characteristics of the three fungi, explain the isolation method from food samples, identify morphological and microscopic differences, and analyze factors that influence the success of isolation and identification. The method used was fungal isolation in culture media from food samples, followed by macroscopic and microscopic observations. Data were analyzed descriptively in the form of tables and graphs. The results showed that Aspergillus sp. had the largest number of colonies (12 colonies) with an average diameter of 2.0 mm, Penicillium sp. had the largest diameter (4.5 mm) with 10 colonies, while Rhizopus sp. had 5 colonies with a diameter of 3.0 mm and the fastest growth. Macroscopically, there were differences in color, texture, and shape of the colonies, while microscopically, differences were seen in the type of hyphae, reproductive structure, and characteristics of each fungus. These differences are influenced by environmental factors such as temperature, humidity, substrate type, and the isolation technique used. In conclusion, the three fungi have distinct characteristics and can be identified through morphological and microscopic observations. Maintaining sterility and using a variety of media and environmental conditions is recommended to improve the accuracy of research result.
Implikasi Patogen Bawaan Makanan yang Resisten terhadap Antibiotik bagi Keamanan Makanan Jalanan di Surakarta, Jawa Tengah, Indonesia Liss Dyah Dewi Arini; Fredericus Pramonodjati; Annisa Rahmawati; Sovia Meldalina; Putri Aulia Ramadhani
Termometer: Jurnal Ilmiah Ilmu Kesehatan dan Kedokteran Vol. 4 No. 3 (2026): Juli : Termometer: Jurnal Ilmiah Ilmu Kesehatan dan Kedokteran
Publisher : Lembaga Pengembangan Kinerja Dosen

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55606/termometer.v4i3.6719

Abstract

The safety of street-vended food is a critical public health issue, as such foods can serve as vehicles for the transmission of pathogenic bacteria that cause foodborne illnesses. This threat is exacerbated by the emergence of antibiotic-resistant bacteria, which complicates the treatment of human infections. This study aimed to assess the impact of drug-resistant pathogenic bacteria on the safety of street food in Surakarta City, Central Java Province, Indonesia. A descriptive laboratory-based method was employed to analyze 20 street food samples collected from various locations in Surakarta. Isolated bacteria were tested for susceptibility using the disk diffusion method (Kirby–Bauer) against four antibiotics: chloramphenicol, levofloxacin, amikacin, and ampicillin. The results revealed varying inhibition zone sizes across the antibiotics, indicating differing levels of bacterial sensitivity. On average, chloramphenicol demonstrated the highest antibacterial activity, followed by ampicillin, amikacin, and levofloxacin. Conversely, certain isolates from specific food samples showed no inhibition zones against any of the tested antibiotics, suggesting the presence of multidrug-resistant (MDR) bacteria. These findings indicate that street food can act as a source for the spread of antibiotic-resistant bacteria, posing potential health risks to the public. Therefore, improvements in food hygiene and sanitation, routine food quality monitoring, and surveillance of antibiotic resistance in read to-sell foods are essential aligned with the "One Health" approach—to mitigate the spread of antimicrobial resistance. The study's results are expected to serve as a scientific basis for refining street food safety management policies and preventing the rise of antibiotic resistance in Indonesia.