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MEDIA ALTERNATIF KACANG TANAH UNTUK PERTUMBUHAN BAKTERI Mily Zamilah; Undang Ruhimat; Doni Setiawan
Journal of Indonesian Medical Laboratory and Science Vol 1 No 1: Oktober 2020
Publisher : Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53699/joimedlabs.v1i1.11

Abstract

The high cost of instant media such as nutrient agar encourages researchers to find alternative media from raw materials that are readily available and cheap. The composition of the media, which is very important for the growth of bacteria, one of which is protein. The content can be obtained from nuts, one of which is groundnut (Arachis hypogaea L). This study aims to determine the growth of Staphylococcus aureus and Escherichia coli bacteria on peanut media which are used as alternative media for Nutrient Agar (NA) substitution. The research method used is Total Plate Count. Escherichia coli bacterial colonies that grew on peanut media were 43 CFU, and colonies that grew on NA media were 238 CFU. While the growth of Staphylococcus aureus bacterial colonies in peanut media was 32 CFU and bacterial colonies growing on NA media were 217 CFU. The conclusion is that peanut media can be used as an alternative media with the growth of Escherichia coli and Staphylococcus aureus bacteria that are in accordance with Standard Plate Count.
Profil Faktor Risiko Kejadian Stroke Berdasarkan Carta Score di Kabupaten Ciamis Siti Rohimah; Tika Sastraprawira; Undang Ruhimat
Jurnal Keperawatan Galuh Vol 5, No 1 (2023): Januari
Publisher : Universitas Galuh Ciamis

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25157/jkg.v5i1.9907

Abstract

Tingkat kecacatan fisik dan mental pada pasien pasca stroke bisa mempengaruhi kualitas hidup pasien. Dampak penurunan fungsional ini menyebabkan penderita stroke kehilangan produktivitasnya dan harus mengeluarkan biaya yang besar untuk perawatan rehabilitasi. Tujuan Penelitian untuk melakukan identifikasi faktor risiko stroke, perilaku berisiko PTM dan risiko kejadian stroke pada komunitas di Kabupaten Ciamis. Jenis penelitian yang digunakan adalah deskriptif kuantitatif dengan jumlah sampel 257 masyarakat yang berada di Kabupaten Ciamis. Instrument yang digunakan adalah kuisioner dan alat ukur tekanan darah, timbangan, pengukur tinggi badan dan stik test pengukur glukosa darah dan kolesterol, risiko kejadian stroke diukur dengan Carta Score. Hasil Penelitian bahwa sebagian besar responden berusia 26 smapai 56 tahun, jenis kelamin 67,7% perempuan, 84.4% merokok, 88,3% makan sayur dan buah kurang dari 5porsi, 55.3% meiliki aktivitas cukup, 53.3% tidak mengalami gangguan tidur dan makan, 42,4 % memiliki IMT buruk, 65% lingkar perut katagori buruk, 45.5% tekanan darah katori buruk, 82.5% GDS katagori baik, 76,7% kadar kolesterol katagori buruk, dan 64,6% memiliki risiko kejadian stroke pada 10 tahun yang akan datah kurang dari 10%. Dapat disimpulan bahwa lebih dari setengahnya masyarakat kabupaten Ciamis memiliki faktor risiko kurang makan sayur dan buah, kadar kolesterol yang buruk, dan lingkar perut yang buruk. Yang dapat meningkatkan risiko kejadian stroke dimasa yang akan datang. Saran: hasil identifkasi berdasarkan wilayah dapat dijadikan sasaran program prioritas dalam mengendalikan faktor risiko stroke.
Almond as Alternative Media for Growth of Staphylococcus aureus and Escherichia coli Ary Nurmalasari; Leni Marlina; Undang Ruhimat; Rahayu Nirmatul Mutmainah
JURNAL KESEHATAN STIKes MUHAMMADIYAH CIAMIS Vol. 9 No. 2 (2022): Jurnal Kesehatan (Oktober 2022)
Publisher : LPPM STIKes Muhammadiyah Ciamis

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52221/jurkes.v9i2.344

Abstract

The high cost of media for microbial culture encourages researchers to create alternative media using local raw materials at low prices. One of the most important media compositions for bacterial growth is protein. This content can be obtained from nuts, one of which is almonds (Prunus dulcis). The purpose of this study   is   to  find  natural   media  that    has   the   potential    for   the    growth of microorganisms at a low price. The  research method  used is an experimental method to determine the growth of Escherichia coli and Staphylococcus aureus bacteria on natural media of almonds. The results showed the growth of Escherichia coli and Staphylococcus aureus bacteria in this alternative media of almonds. Based on the results of the study, it can be concluded that  almond  nut  media  can  be  applied  as  an  alternative  medium in   laboratory research, especially in the field of_microbiology. It is suggested that further research should be carried out on natural media which are sources of carbohydrates and proteins as alternative growth media for bacteria.
Identification Coliform Bacteria In Student Drinking Water In The Al-Mu'aawanah Boarding School, District Rajadesa, Ciamis District Undang Ruhimat; Rivana Ariyadi; Rahayu Nirmatul; Fatna Agnianisa Salsabila
Mukhtabar Journal of Medical Laboratory Technology Vol 1 No 1 (2023): Mukhtabar: Journal of Medical Laboratory Technology (April 2023)
Publisher : LPPM STIKes Muhammadiyah Ciamis

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52221/mjmlt.v1i1.207

Abstract

Background & Objective: Drinking water is a very important human need. Drinking water that is suitable for consumption must meet established standards, among others, according to the Minister of Health of the Republic of Indonesia No.492/Menkes/IV/2010. This study aims to determine the presence or absence of coliform bacteria in the drinking water of students using the MPN method. Methods: This type of research is descriptive. The sampling was carried out at the Al-Mu'aawanah Islamic boarding school. The results showed that the samples of drinking water for male and female students for microbiological parameters showed that the samples were positive for coliform bacteria. The sample for female students was 22/100 mL, and the sample for male students was 2/100 mL. Based on Permenkes No.472/Menkes/IV/2010, the maximum total concentration of coliform bacteria is 0/100 mL, which means that it is not in accordance with the standards of Permenkes No.472/Menkes/IV/2010, and the growth of faecal coliform bacteria was found in EMB media in both samples. namely the metallic green Escherichia coli bacteria, which indicates that the sample does not meet the microbiological requirements of drinking water.
Description of Candida albicans Fungus in The Oral Mucosa of Patients With Fe Deficiency Anemia in RSUD Ciamis Aditya Nur Romadhon; Mei Widiati; Undang Ruhimat; Euis Tia Istianah
Mukhtabar Journal of Medical Laboratory Technology Vol 1 No 1 (2023): Mukhtabar: Journal of Medical Laboratory Technology (April 2023)
Publisher : LPPM STIKes Muhammadiyah Ciamis

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52221/mjmlt.v1i1.217

Abstract

Background & Objective Indonesia is a developing country where Fe deficiency anemia is common. Fe deficiency anemia is a malnutrition disease caused by a lack of Fe in the patient's body. Fe deficiency anemia is triggered by a lack of Fe in the body for the formation of hemoglobin, causing patients to experience blood deficiency and thinning of oral mucosal epithelial cells which can cause oral candidiasis. Analyzing the description of oral candidiasis in patients with Fe deficiency anemia. Methods  This study is a descriptive study that recruited a total sampling of 17 patients with Fe deficiency anemia. Oral candidiasis in Fe deficiency anemia was measured by planting on sabouraud dextrose agar (SDA) media and sugars media then observed macroscopically and microscopically from SDA and sugars cultures using a microscope. Results Based on the results of the study, 15 (88%) positive respondents and 2 (12%) negative respondents. Conclusion The results of the examination of oral candidiasis as many as 15 samples were affected by oral candidiasis caused by Candida albicans.
Screening of Pulmonary Tuberculosis in Household Contacts With Acid Fast Bacteria (AFB) Positive Pulmonary Tuberculosis Patients in Ciwangi Village, Balubur Limbangan Subdistrict, Garut District Shinta Nuriah; Undang Ruhimat; Rivana Aryadi; Rahayu Nirmatul Mutmainah
Mukhtabar Journal of Medical Laboratory Technology Vol 1 No 1 (2023): Mukhtabar: Journal of Medical Laboratory Technology (April 2023)
Publisher : LPPM STIKes Muhammadiyah Ciamis

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52221/mjmlt.v1i1.218

Abstract

Background & Objective Tuberculosis is an infectious disease caused by acid-fast bacteria, namely Mycobacterium tuberculosis, which mainly attacks the lungs. Pulmonary tuberculosis is a global health problem with the second deadliest case after HIV/AIDS infection, and ranks third with the most TB cases after India and China. The main source of transmission is patients with active TB pulmonary tuberculosis who can transmit to people around them. This study aims to determine whether pulmonary TB transmission occurs in people who are in household contact with patients with acid-fast bacteria pulmonary TB active. Method This study is descriptive, using the Ziehl neelsen examination method, with a total sampling technique, conducted on 34 people who are in household contact with pulmonary TB patients in Ciwangi Village, Balubur District, Limbangan. Result Based on the examination results in people who are in contact with people with TB, the results are negative, the presence of the bacteria that cause TB. Conclusion The results of Pulmonary Tuberculosis screening conducted on 34 people from 13 heads of families, it can be concluded that the results of TB microscopy with the Ziehl neelsen technique were negative.
Erythrocyte Index and Mentzer Index in Fathers of Thalassemia Patients Rhama Adiguna; Doni Setiawan; Undang Ruhimat; Rahayu Nirmatul Mutmainah
Mukhtabar Journal of Medical Laboratory Technology Vol 1 No 1 (2023): Mukhtabar: Journal of Medical Laboratory Technology (April 2023)
Publisher : LPPM STIKes Muhammadiyah Ciamis

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52221/mjmlt.v1i1.219

Abstract

Background & Objective Thalassemia is a blood disorder caused by genetic factors that cause the globin chain in hemoglobin to not function normally. It is the most common hereditary disease in the world and in Ciamis Regency in 2021 there were 191 patients. Thalassemia prevention is carried out by screening to identify thalassemia carriers. Hematological examination in the form of erythrocyte index (MCV, MCH, MCHC) and mentzer index has been known to determine thalassemia screening.  The purpose of this study was to determine the description of erythrocyte index and mentzer index in thalassemia patient's father. Method The method used in this study is descriptive method. The population in this study were members of POPTI (Parents Association of Thalassemia Patients) Ciamis Regency. The sample in this study were 30 samples. Result Based on the results of the study obtained the results of erythrocyte index (MCV, MCH) low (MCHC) normal and Mentzer index obtained the results of 20 samples (66%) suspected as thalassemia carrier, 5 samples (17%) suspected iron deficiency anemia and according to the reference value there were 5 samples (17%). Conclusion The results of a study conducted on 30 fathers of thalassemia patients showed that 83% of fathers of thalassemia patients had MCV and MCH values below the normal threshold and 73% had normal MCHC values. Furthermore, from the results of the Mentzer index examination, it is known that 66% of the fathers of thalassemia patients are suspected of having minor thalassemia, 17% are suspected of having iron deficiency anemia disorders and 17% have a normal Mentzer index.
Overview of Total Cholesterol Levels at Productive Age in the Imbanagara Health Center Working Area Intan Fandini; Undang Ruhimat; Rivana Ariyadi; Rahayu Nirmatul Mutmainah
Mukhtabar Journal of Medical Laboratory Technology Vol 1 No 2 (2023): Mukhtabar: Journal of Medical Laboratory Technology (October 2023)
Publisher : LPPM STIKes Muhammadiyah Ciamis

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52221/mjmlt.v1i2.368

Abstract

Background & Objective: Total cholesterol is the total amount of HDL, LDL, and VLDL cholesterol in each deciliter of blood. Risk factors that can affect total cholesterol levels are age, gender, sleep patterns, genetics, physical activity, and body mass index. This study aims to determine the description of total cholesterol levels in productive age in the Imbanagara Health Center Working Area. Method: This research method is descriptive research with a purposive sampling technique and involves 31 respondents the instrument used is a photometer. This research was conducted in the Imbanagara Health Center working area from May to June 2023. Result: The results showed that the total cholesterol levels of 31 respondents, were 25 people (81%) with normal total cholesterol levels, 5 people (16%) with high threshold total cholesterol levels, and 1 other person (3%) with high total cholesterol levels. Conclusion: It can be concluded that of the 31 respondents in the Imbanagara Health Center work area, the average has normal Total Cholesterol results.
Identification of Staphylococcus aureus Bacteria on the Palms of Visitors to Panumbangan Health Center Rivana Ariyadi; Putri Agnia Maulani; Undang Ruhimat; Rudy Hidana
Mukhtabar Journal of Medical Laboratory Technology Vol 1 No 2 (2023): Mukhtabar: Journal of Medical Laboratory Technology (October 2023)
Publisher : LPPM STIKes Muhammadiyah Ciamis

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52221/mjmlt.v1i2.376

Abstract

Background & Objective: Hand hygiene is one of the efforts to prevent and control the spread of bacteria that can cause infectious diseases. Nosocomial infections can be caused by gram-positive bacteria, such as Staphylococcus aureus which is the most dominant bacterium causing skin and soft tissue infections. The purpose of this study was to determine the presence of Staphylococcus aureus bacteria in the palms of visitors to the Panumbangan Health Center. Method: This study is a descriptive observational study with a simple random sampling technique conducted from May to June 2022.  Respondents in this study were 20 health center visitors. Sampling was carried out at the Panumbangan Health Center and the examination was carried out at the STIKes Muhammadiyah Ciamis Bacteriology Laboratory. The measurement methods used were gram staining, media culture, and biochemical tests. The data obtained from the examination results were presented descriptively in tabular form and explained in narrative. Result: The results showed that 6 samples (30%) identified Staphylococcus aureus, 11 samples (55%) Staphylococcus epidermidis, and 3 samples (15%) suspected Streptococcus sp.   Conclusion: It can be concluded that 30% of Staphylococcus aureus, 55% of Staphylococcus epidermidis, and 15% of Streptococcus sp. were identified.
Overview of Haemoglobin Levels Before and After Menstruation in Female Students of STIKes Muhammadiyah Ciamis Undang Ruhimat; Irma Miratul Hasanah; Endang Octaviana Wilujeng; Rosmiati Rosmiati
Mukhtabar Journal of Medical Laboratory Technology Vol 1 No 2 (2023): Mukhtabar: Journal of Medical Laboratory Technology (October 2023)
Publisher : LPPM STIKes Muhammadiyah Ciamis

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52221/mjmlt.v1i2.378

Abstract

Background & Objective: Menstruation is the periodic discharge of blood and body cells from the uterine wall of the vagina. The effect of menstruation is the risk of anemia in adolescents. The appearance of menstrual anemia will hurt the growth and development of adolescent girls, such as decreased concentration and learning ability, impaired growth, resulting in unsatisfactory height, decreased physical strength, and pale skin. This study aimed to determine the difference in Haemoglobin levels before and after menstruation in female students of STIKes Muhammadiyah Ciamis DIII Health Analyst Study Program. Method: This research is a descriptive study with a purposive sampling technique conducted from January to June 2021. Respondents in this study were level 2 female students year students of the D-III Health Analyst Study Program at STIKes Muhammadiyah Ciamis as many as 31 respondents—were measured using the CyanmetHaemoglobin method with the instrument used, namely the Photometer tool. The examination results were processed manually and displayed in table format for narration. Result: The examination results of Haemoglobin levels before menstruation averaged 13.1 g/dL and Haemoglobin levels after menstruation averaged 11.9 g/dL. Conclusion: It can be concluded that the average results show a decrease in Haemoglobin levels when experiencing menstruation.