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All Journal Majalah Kedokteran Bandung BIOTROPIA - The Southeast Asian Journal of Tropical Biology Jurnal Biosains Pascasarjana Jurnal Riset Industri Jurnal Ilmiah Arena Tekstil Narra J Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek) International Journal of Health Science and Technology Sciences of Pharmacy Jurnal Kajian Budaya dan Humaniora (JKBH)
Ratu Safitri
Mikrobiologi – Biologi UNPAD
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Differential Regulation of Slc40a1, Fth1, and Hmox1 by Deferasirox in Splenic Iron Overload Wibowo, Annisa Maharani; Kuntana, Yasmi Purnamasari; Arrizqiani, Tanendri; Safitri, Ratu
Sciences of Pharmacy Volume 4 Issue 4
Publisher : ETFLIN Publishing House

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58920/sciphar0404386

Abstract

Iron overload, often arising from repeated transfusions in thalassemia major, disrupts iron homeostasis and induces oxidative stress. Deferasirox is a widely used oral chelator, yet its effects on splenic iron-regulatory gene expression remain unclear. This study investigated the impact of deferasirox on ferritin heavy chain (Fth1), ferroportin (Slc40a1), and heme oxygenase-1 (Hmox1) expression in a rat model of splenic iron overload. Eighteen male Wistar rats were randomly assigned into three groups (n = 6 each): normal (N), iron dextran-induced overload without treatment (KN), and iron overload treated with deferasirox (KP). Gene expression was quantified by real-time PCR using the 2−ΔΔCT (Livak) method, with statistical analysis performed via one-way ANOVA and Tukey’s post hoc test. Iron overload significantly upregulated Fth1 (2.26-fold) and Slc40a1 (1.72-fold) versus controls (p < 0.05). Deferasirox treatment reduced Fth1 (3.28-fold decrease) and Slc40a1 (1.15-fold reduction) relative to untreated overload, though not significantly (p > 0.05). In contrast, Hmox1 expression markedly increased (55.25-fold, p < 0.05) following deferasirox administration. These results indicate that deferasirox selectively modulates splenic iron-regulatory genes, suggesting both chelation and adaptive stress-response mechanisms, thereby supporting its therapeutic role in managing iron overload.
EFFECT OF POWDER AND LIQUID PREPARATIONS OF PROBIOTICS ON WHITE SHRIMP (Litopenaeus vannamei) GROWTH PERFORMANCE Safitri, Ratu; Andriani, Yuli; Sunendi, Sunendi; Iskandar, Iskandar; Buwono, Ibnu Dwi
BIOTROPIA Vol. 27 No. 3 (2020): BIOTROPIA Vol. 27 No. 3 December 2020
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.0.0.0.1124

Abstract

Indonesia is the second largest supplier of white shrimp (Litopenaeus vannamei, Boone 1931) in the USA market. Hence, the need for its sustainable production and improved growth. Probiotics, among others, are known for their growth enhancing attributes. Therefore, this study was conducted to determine the effects of powder and liquid probiotics on the growth of white shrimps at the Minaloka Jaya shrimp ponds, Grabag District, Purwerojo Regency, Central Java. The shrimps were cultivated for 60 days and applied with three probiotic treatments, namely commercial liquid probiotics with dosage of 10 mL/kg feed, powder probiotics with dosage of 10 g/kg feed and liquid probiotic with dosage of 10 mL/kg feed. Each probiotic preparation was administered four times a day to over 150,000 vannamei shrimps which were cultured in a semi-intensive system. Probiotics in powder and liquid forms contain Lactobacillus fermentum, L acidophilus, L. plantarum, L, curvatus, Bacillus licheniformis, B. subtilis, and B. polimyxa. B. megaterium, B. coagulans, Pseudomonasputida, Nitrosomonas sp. and Nitrobacter sp. Using the Randomized Block Design (RBD), the three treatments were replicated five times. The application of probiotics in both powder and liquid forms had increased the growth yield of the vannamei shrimp. However, the powder probiotic had shown better growth performance than the commercial liquid probiotics and liquid preparation of probiotics. Probiotic powder form provides a specific growth rate (SGR) of 8.18%, absolute body length of 9.68 cm, absolute biomass of 6.78 g, and feed conversion ratio (FCR) of 1.93.
Effect of Deferiprone on Hepatic Expression of Hamp, Ftl, and Tfr1 Genes in an Iron-Overloaded Rat (Rattus norvegicus) Model Salsabila, Nadhila Hasna; Kuntana, Yasmi Purnamasari; Arrizqiyani, Tanendri; Safitri, Ratu
Sciences of Pharmacy Volume 4 Issue 4
Publisher : ETFLIN Publishing House

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58920/sciphar0404387

Abstract

Iron overload is linked to progressive impairment of organ function, with the liver being the primary site of deposition due to the lack of a physiological route for iron elimination. The maintenance of systemic iron balance depends on key regulatory proteins, including hepcidin (Hamp gene), ferritin light chain (Ftl gene), and transferrin receptor 1 (Tfr1 gene). This study tested the hypothesis that Deferiprone (DFP), an oral iron chelator, modulates the hepatic expression of Hamp, Ftl, and Tfr1 genes in an iron-overloaded rat model. Eighteen male Wistar rats (150-200 g) were randomly assigned into three groups: Normal (N), Negative Control (NC; induced with Iron Dextran), and Treatment (T; Iron Dextran + DFP). Iron overload was induced via intravenous injection of Iron Dextran (120 mg/kg BW) over 15 days at 3-day intervals, while DFP was administered orally (100 mg/kg BW) in three divided doses for 28 consecutive days. Gene expression was assessed using RT-PCR, and relative quantification was performed using the Livak method. The iron-overloaded rats showed marked upregulation of Hamp and Ftl and downregulation of Tfr1. Administration of DFP significantly reversed these alterations, decreasing Hamp and Ftl levels while restoring Tfr1 expression to levels comparable to normal controls. These results highlight the potential role of DFP in modulating hepatic iron-regulatory genes under iron overload conditions. 
Co-Authors Ani Melani Maskoen Arrizqiani, Tanendri Bashari, Muhammad H. Edhyana Sahiratmadja Fakhira, Anisa Muthia Firdawati, Nurul Fitri Rahmi Fadhilah Gemilang Lara Utama Herman Susanto Ibnu Dwi Buwono Irianto, Gunawan Iskandar Iskandar Jeri Nobia Purnama Kamisah, Yusof Khristian, Erick Kuntana, Yasmi Purnamasari Melanie, M Mia Miranti Mohammad Ghozali, Mohammad Mutia, Theresia Nurullia Fitriani Purnama, Jeri N. Ramdan Panigoro Rifaida Eriningsih, Rifaida Rusdianto Rusdianto, Rusdianto Salsabila, Nadhila Hasna Sunendi, Sunendi Tanendri Arrizqiyani, Tanendri Theresia Mutia, Theresia Wibowo, Annisa Maharani Yasmi P. Kuntana, Yasmi P. Youngest, Racy Yuli Andriani Yulia, Tri