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All Journal Jurnal Veteriner Majalah Kedokteran Bandung Althea Medical Journal Medical Journal of Indonesia Jurnal Farmasi Klinik Indonesia Global Medical and Health Communication MNJ (Malang Neurology Journal) The Indonesian Biomedical Journal Universa Medicina Paediatrica Indonesiana Journal of the Indonesian Medical Association : Majalah Kedokteran Indonesia Narra J Cermin Dunia Kedokteran Jurnal Neuroanestesi Indonesia Makara Journal of Health Research Cermin Dunia Kedokteran
Ramdan Panigoro
Biochemistry And Molecular Biology Division, Department Of Biomedical Sciences, Faculty Of Medicine, Universitas Padjadjaran, Bandung
Biochemistry, Genetics & Molecular Biology Dentistry Education Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Nursing Public Health Veterinary

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Nasopharyngeal bacterial carriage and antimicrobial resistance in underfive children with community acquired pneumonia Kartasasmita, Cissy B.; Duddy, Heda M.; Sudigdoadi, Sunaryati; Agustian, Dwi; Setiowati, Ina; Ahmad, Tri H.; Panigoro, Ramdan
Medical Journal of Indonesia Vol 11, No 3 (2002): July-September
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (227.086 KB) | DOI: 10.13181/mji.v11i3.70

Abstract

Pathogens in nasopharynx is a significant risk factor of pneumonia. According to WHO, isolates to be tested for antimicrobial resistance in the community should be obtained from nasopharyngeal (NP) swabs. The aim of this study is to know the bacterial patterns of the nasopharynx and cotrimoxazole resistance in under five-year old children with community acquired pneumonia. The study was carried out in 4 primary health clinic (Puskesmas) in Majalaya sub-district, Bandung, West Java, Indonesia. All underfive children with cough and/or difficult breathing and classified as having non-severe pneumonia (WHO guidelines) were placed in Amies transport medium and stored in a sterile jar, before taken to the laboratory for further examination, in the same day. During this nine month study, 698 children with clinical signs of non-severe pneumonia were enrolled. About 25.4% (177/698) of the nasopharyngeal specimens yielded bacterial isolates; i.e. 120 (67.8%) were positive for S pneumoniae, 21 for S epidermidis and alpha streptococcus, 6 for Hafnia alvei, 5 for S aureus, 2 for B catarrhalis, and 1(0.6%) for H influenza and Klebsiella, respectively. The antimicrobial resistance test to cotrimoxazole showed that 48.2% of S pneumoniae strain had full resistance and 32.7% showed intermediate resistance to cotrimoxazole. This result is almost similar to the other studies from Asian countries. It seems that H influenza is not a problem in the study area, however, a further study is needed. (Med J Indones 2002; 11: 164-8) Keywords: nasopharyngeal swab, S pneumoniae, cotrimoxazole
Increased knowledge of thalassemia promotes early carrier status examination among medical students Dewanto, Julius Broto; Tansah, Haryono; Dewi, Sari Puspa; Napitu, Helena; Panigoro, Ramdan; Sahiratmadja, Edhyana
Universa Medicina Vol 34, No 3 (2015)
Publisher : Faculty of Medicine, Trisakti University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/UnivMed.2015.v34.220-228

Abstract

BACKGROUNDThalassemia is an autosomal recessive genetic disorder, in which the patient requires life-long blood transfusion. As Indonesia harbors 6 to 10% thalassemia carriers, thalassemia prevention measures such as early screening and education in the community are urgently needed. The aim of this study was to explore the knowledge, attitude and practice about thalassemia among young medical students. METHODSA cross-sectional analytic observational study was conducted on 179 subjects in 2015, using a questionnaire with items on knowledge, attitude and practice about thalassemia for data collection. After signing informed consent, the questionnaire was filled in by the students and a blood test was performed when the students agreed to be examined. Detection of probable thalassemia carrier status was done by determination of hemoglobin, mean corpuscular volume and mean corpuscular hemoglobin. RESULTSThe knowledge about thalassemia of the first year medical students (n=179) was good (21.1%), moderate (70.9%) and poor (21.1%). Only 67 (38.3%) of the students agreed to a blood examination for determination of their carrier status after filling-in the questionnaire. The knowledge of thalassemia among first year medical students was statistically related to the timing when they would agree to have their thalassemia carrier status examined (p=0.021, one way ANOVA test). CONCLUSIONA higher thalassemia knowledge score causes medical students to be willing to undergo thalassemia carrier status examination at an earlier point in timing. A well-organized educational program focusing on thalassemia and early screening in young adults may enhance the thalassemia prevention program.
Factors Affecting Mortality in Adult Tetanus Patients Khrisnan, Lohghinee; Ong, Anam; Panigoro, Ramdan
Althea Medical Journal Vol 2, No 2 (2015)
Publisher : Althea Medical Journal

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Abstract

Background: Tetanus is a devastating disease that is associated with high mortality. The aim of this study was to analyze the prognostic factors that were associated with high mortality in tetanus. Methods: This was an analytical study and the data was collected retrospectively from 1 January 2010–31October 2012 in the Department of Neurology, Dr. Hasan Sadikin General Hospital.Results: Out of hundred thirty two cases only 87 cases of tetanus patient have complete medical records. There were 54 male and 33 female. The age range was 35-49 years old. Mortality rate was high (32.18%). The most frequent wound site is extremities (85.07 %). The absence of post-injury tetanus vaccination was 75.86%, period of onset <48 hours was 67.82% and the use of mechanical ventilation and tracheostomy 36.07%. Mortality was significantly associated with an incubation period <7 days (p<0.05), presence of fever(p <0.05), co morbidity of autonomic storm (p<0.01), and the severity of the disease by grade 5 (p<0.05).Conclusions: Factors affecting the mortality in adult tetanus patients were significant for incubation period, fever, co morbidity and severity of the disease by grade. [AMJ.2015;2(1):157–61]
Status Asetilator Gen NAT2 pada Pasien Tuberkulosis dan Tuberkulosis dengan Diabetes Melitus di Kupang, Nusa Tenggara Timur Pramono, Alvinsyah Adhityo; Penggoam, Simeon; Sahiratmadja, Edhyana; Utami, Novi Vicahyani; Achmad, Tri Hanggono; Panigoro, Ramdan
Majalah Kedokteran Bandung Vol 49, No 1 (2017)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (588.875 KB) | DOI: 10.15395/mkb.v49n1.989

Abstract

Indonesia adalah negara dengan jumlah penderita tuberkulosis (TB) terbanyak kedua  di dunia. Diabetes melitus (DM) merupakan salah satu komorbid TB. Arylamine N-acetyltransferase 2 (NAT2) adalah enzim yang berfungsi memetabolisir isoniazid (INH) yang disandi oleh gen NAT2. Gen NAT2 memiliki sejumlah polimorfisme dan dapat menentukan kemampuan seseorang untuk memetabolisir obat yang disebut status asetilator. Pada individu dengan status asetilator lambat, INH dimetabolisir dengan lambat sehingga memungkinkan terjadi intoksikasi hati. Pada TB dengan DM (TBDM) status asetilator lambat dapat membuat pengobatan TB maupun DM menjadi kurang optimal. Penelitian ini bertujuan mengeksplorasi status asetilator pasien TBDM di RSUD Prof. WZ Johannes Kupang periode Juni–November 2011. Pada penelitian potong lintang ini DNA dari darah 122 pasien TB diisolasi dan gen NAT2 kemudian diamplifikasi dan disekuensing untuk diketahui status asetilatornya. Hasil penelitian menunjukkan terdapat 5 pasien yang memiliki glukosa serum >200 mg/dL yang dikategorikan sebagai pasien TBDM. Pada pasien TBDM didapatkan seorang dengan status asetilator cepat (NAT2*4/NAT2*4), 2 orang dengan status asetilator sedang (NAT2*13A/NAT2*6J), dan 2 orang dengan status asetilator lambat (NAT2*5/NAT2*5G, NAT2*6A/ NAT2*6A, NAT2*7B/ NAT2*7B). Pada pasien TB yang dipilih secara random berdasar usia dan jenis kelamin serupa dengan TBDM didapatkan 2 orang dengan status asetilator cepat (NAT2*4/NAT2*4) dan 3 orang dengan asetilator sedang (NAT2*4/NAT2*6A, NAT2*13A/NAT2*6J). TBDM yang memiliki status asetilator lambat berpotensi memiliki masalah ganda dalam terapi, selain dapat terjadi toksisitas hati akibat terapi dengan INH, juga dapat mengakibatkan pengobatan DM menjadi tidak optimal. Perlu dilakukan peneltian lebih lanjut terkait farmakogenetik pada TBDM. [MKB. 2016;49(1):61–6]Kata kunci: Asetilator, isoniazid, NAT2, farmakogenetik, tuberkulosis NAT2 Gene Acetylator Status of Tuberculosis and Tuberculosis with Diabetes Mellitus Patients in Kupang, Nusa Tenggara TimurIndonesia is the second highest country with TB patients in the world. Diabetes mellitus (DM) is a comorbid of TB. Arylamine N-acetyltransferase 2 (NAT2), encoded by the NAT2 gene, is an enzyme that metabolizes isoniazid (INH). NAT2 gene has some polimorphysims that may play a role in INH acetylating process. Those who are slow acetylators may develop liver intoxication as a consequence of slow INH metabolism process. Slow acetylator TBDM patients may complicate both TB and DM treatment, causing them to be less optimal. The aim of this study was to explore the acetylator status of TBDM patients in Kupang, Indonesia. A cross-sectional study was conducted by obtaining DNA of 122 TB patients in Kupang in June–November 2011. NAT2 gene was amplified and sequenced to determine the acetylator status. There were 5 TB patients who had a glucose serum level of >200mg/dL and was catagorized as TBDM. Result showed that there was 1 TBDM patient who was a rapid acetylator (NAT2*4/NAT2*4), 2 patients as intermediate acetylators (NAT2*13A/NAT2*6J), and 2 patients as slow acetylators (NAT2*5/NAT2*5G, NAT2*6A/ NAT2*6A, NAT2*7B/ NAT2*7B). Meanwhile,  there were 2 TB patients who was rapid acetylators (NAT2*4/NAT2*4) and 3 patients as intermediate acetylators (NAT2*4/NAT2*6A, NAT2*13A/NAT2*6J). Slow NAT2 acetylator TBDM patients potentially face more problems during therapy. As INH may cause liver intoxication, these patients may also experience unoptimum DM treatment. Therefore, it is strongly recommended to do a study on the role of pharmacogenomics in TBDM. [MKB. 2016;49(1):61–6]Key words: Acetylator, isoniazid, NAT2, pharmacogenetics, tuberculosis
Metode Cepat Identifikasi Flavonoid dari Daun Ocimum sanctum L. (Selasih) Dhianawaty, Diah; Panigoro, Ramdan; Surialaga, Samsudin; Purushothman, Pricilla
Majalah Kedokteran Bandung Vol 44, No 1 (2012)
Publisher : Faculty of Medicine, Universitas Padjadjaran

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Abstract

Manfaat suatu tumbuhan sebagai obat herbal diperoleh dari kandungan kimianya, misalnya flavonoid. Flavonoid bermanfaat untuk kesehatan tubuh manusia. Oleh karena itu, kandungan flavonoid dapat dijadikan sebagai acuan manfaat suatu tanaman dan dibutuhkan metode identifikasi flavonoid yang cepat. Tujuan penelitian untuk memperoleh metode cepat kandungan flavonoid dalam Ocimum sanctum. Telah dilakukan penelitian ekstraksi, isolasi, dan identifikasi flavonoid daun Ocimum sanctum di laboratorium Kimia Medik Fakultas Kedokteran Universitas Padjadjaran pada tahun 2005. Flavonoid diekstraksi sinambung dengan etanol 95%, diisolasi dengan kromatografi kertas/lapis tipis selulose dan asam asetat 2%, kemudian dilanjutkan dengan pengembang: n-butanol–asam asetat–air, kloroform–asam asetat–air, forestall, asam asetat 5%, 15%, 35%, dan 50%. Flavonoid diidentifikasi dengan pereaksi geser dan sinar ultraviolet. Hasil isolasi dengan asam asetat 2% dan 35% berturut-turut memberikan dua bercak flavonoid, sebagai flavon, FOAc-1 mempunyai Rf=0,69 dan FOAc-2 mempunyai Rf=0,57. Pengembang lainnya memberikan satu bercak flavonoid. Simpulan, isolasi dengan asam asetat 2% dan 35% berturut-turut serta identifikasi dengan pereaksi geser dan sinar ultraviolet merupakan metode yang cepat untuk identifikasi kandungan flavonoid dalam Ocimum sanctum. [MKB. 2012;44(1):32–7]. Kata kunci: Flavonoid, identifikasi flavonoid, Ocimum sanctumRapid Identification Method of Flavonoid from Ocimum sanctum L. (Selasih) Leaves A plant’s effectiveness as a herbal drug comes from its chemical content such as flavonoids. Flavonoids are useful for human body health. Therefore flavonoids content can be used as a marker from the usefulness of a plant, and rapid identification method of flavonoid is needed. The objective of the research was to get a rapid method of flavonoid content identification from Ocimum sanctum. The extraction, isolation and identification of flavonoids from Ocimum sanctum leaves has been done at Medical Chemistry laboratory Faculty of Medicine Universitas Padjadjaran in 2005. Flavonoids were extracted continually by 95% ethanol, were isolated with paper chromatography/cellulose TLC and 2% acetic acid, then continued with elucidation reagents: n-butanol–acetic acid–water, chloroform–acetic acid–water, forestall, 5%, 15%, 35% and 50% acetic acids. Flavonoids were identified with diagnostic reagents and ultraviolet light. Isolation with 2% and 35% acetic acids respectively gave two spots of flavonoids as flavon, FOAc-1 had Rf=0.69 and FOAc-2 had Rf=0.57. The other elucidation reagents gave one spot of flavonoid. In conclusion, isolation with 2% and 35% acetic acids respectively and identification with diagnostic reagent and ultraviolet light is a rapid method for identification of flavonoids content in Ocimum sanctum. [MKB. 2012;44(1):32–7].Key words: Flavonoid, identification of flavonoid, Ocimum sanctum DOI: http://dx.doi.org/10.15395/mkb.v44n1.73
COGNITIVE IMPAIRMENT DETECTION IN ADULT THALASSEMIA PATIENT USING MOCA-INA Wardoyo, Chandra Calista; Gamayani, Uni; Ong, Anam; Rizal, Ahmad; Wibisono, Yusuf; Amalia, Lisda; Panigoro, Ramdan
MNJ (Malang Neurology Journal) Vol. 7 No. 1 (2021): January
Publisher : PERDOSSI (Perhimpunan Dokter Spesialis Saraf Indonesia Cabang Malang) - Indonesian Neurological Association Branch of Malang cooperated with Neurology Residency Program, Faculty of Medicine Brawijaya University, Malang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.mnj.2021.007.01.6

Abstract

Background: Cognitive impairment in thalassemia patients are prevalent, therefore early detection of cognitive impairment in adult thalassemia patients is crucial for prevention. Montreal Cognitive Assessment (MoCA) is a public domain cognition screening tools that covers all cognitive domains in detecting mild cognitive impairments.Objective: To compare cognitive function between adult thalassemia patients and healty control by using Indonesia version of MoCA test (MoCA-Ina)Methods: This prospective observational analytic with case control study, compared the total scores and scores of each domain of cognition between adult thalassemia patients and healthy subjects at the Medical Hematology Oncology Clinic of Dr. Hasan Sadikin General Hospital, Bandung, Indonesia using MoCA-Ina from August to October 2018.Results: A total of 32 thalassemia subjects and 50 healthy subjects were conducted. A total of 16(50%) subjects in the case group had a value of MoCA-Ina <26, while only 1(2%) healthy control had a value of MoCA-Ina <26. The median total MoCA-Ina score in case and control groups were 25.5 and 27.50 (p <0.001). The median score of memory domains, executive functions and visuospatial of the case and control groups were 3 versus 4 (p <0.001), 3 versus 3.5 (p <0.001) and 3.53 ± 0.671 versus 3.88 ± 0.385 (p <0.003), respectively.Conclusion: Adults thalassemia patients have lower score in total MoCA-Ina, domains of memory, executive function and visuospatial score compared to healthy control.
Poor knowledge of anemia and sexually transmitted disease but better knowledge of HIV/AIDS among unmarried adolescents aged 15-24 years Sahiratmadja, Edhyana; Raharja, Mugia Bayu; Setiawati, Elsa Pudji; Panigoro, Ramdan
Universa Medicina Vol. 40 No. 2 (2021)
Publisher : Faculty of Medicine, Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/UnivMed.2021.v40.110-120

Abstract

BACKGROUND Basic knowledge of sexual and reproductive health is important to be introduced during the premarital period, to reduce high-risk sexual behavior among adolescents, as well as to increase the correct attitude in their future marriage. This study explored the knowledge of HIV/AIDS, sexually transmitted diseases (STDs) and anemia among unmarried adolescents. METHODS This was a cross-sectional analytical study on secondary data from the Indonesian Demographic and Health Population Survey 2017, initially conducted by the National Population and Family Planning Board (BKKBN). Knowledge of HIV/AIDS, STDs and anemia among unmarried adolescents aged 15-24 years in West Java Province and Jakarta, the capital city of Indonesia, was compared and analyzed using the chi-square test. RESULTS In total, 5,389 weighted data were retrieved. In general, adolescents in Jakarta had better knowledge of all diseases compared to those in West Java (p<0.001). Knowledge of anemia and STDs was poor compared to that of HIV/AIDS which was moderate to good (p<0.001). Interestingly, although the adolescents had a middle-low wealth index (43%), the majority (>90%) had their own mobile phone with almost daily internet access, however most of their information sources on health education were from their school period. CONCLUSION Premarital education in schools is imperative to enhance the knowledge of STDs and anemia. Moreover, poor knowledge of anemia is a challenge for introducing the topic of thalassemia major, an inherited autosomal recessive type of anemia due to hemoglobinopathy. Since Indonesia is harboring 6-10% of thalassemia carriers, integrative premarital education is needed for better family planning.
Incidence of Peripheral Neuropathy in Major Beta-Thalassemia Patients at Hasan Sadikin General Hospital, Bandung, Indonesia Putri, Fanny Adhy; Gamayani, Uni; Lailiyya, Nushrotul; Panigoro, Ramdan
Cermin Dunia Kedokteran Vol 46, No 9 (2019): Neuropati
Publisher : PT. Kalbe Farma Tbk.

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (610.493 KB) | DOI: 10.55175/cdk.v46i9.416

Abstract

Bakground. Beta major thalassemia is the most common inherited blood disorder worldwide. It can lead to various neurological complications such as peripheral neuropathy. Toronto Clinical Neuropathy Score (TCNS) is helpful for peripheral neuropathy screening especially in diabetes mellitus. Objective. To investigate the prevalence of peripheral neuropathy in beta major thalassemia patient using Toronto Clinical Neuropathy Score (TCNS). Method. A descriptive study on beta major thalassemia patients aged more than 14 years who regularly underwent blood transfusions in Hasan Sadikin General Hospital Bandung, from July to August 2017. Normal TCNS values was < 4, mild neuropathy 5-7, moderate neuropathy 8-10 and severe neuropathy > 10. Results. Sixty subjects met the inclusion criteria, 48,3% were male with the mean (SD) age of 20,7 ± 7,6 years. Mean hemoglobin value was 6,7 ± 0,9 g/dL and median (IQR) blood ferritin serum was 2873 (1900-3859) μg/L. Thirty-two subjects had neuropathy; 19 (31,7%) with mild neuropathy and 13 (21,6%) with moderate neuropathy.Conclusion. The incidence of peripheral neuropathy in patients with thalassemia according to TCNS score is fairly high.Latar Belakang. Talasemia beta mayor adalah kelainan darah bawaan paling umum di dunia dan dapat menyebabkan berbagai komplikasi, salah satunya neuropati perifer. Toronto Clinical Neuropathy Score (TCNS) dapat digunakan untuk penilaian neuropati perifer yang disebabkan oleh berbagai penyakit sistemik. Objektif. Menyelidiki prevalensi neuropati perifer pada pasien talasemia beta mayor menggunakan Toronto Clinical Neuropathy Score (TCNS). Metode. Penelitian ini studi deskriptif skrining menggunakan TCNS pada pasien thalassaemia beta mayor berusia lebih dari 14 tahun yang secara teratur menjalani transfusi darah di Rumah Sakit Umum Hasan Sadikin Bandung, dari Juli hingga Agustus 2017. Nilai TCNS normal adalah <4, neuropati ringan 5-7, neuropati sedang 8-10 dan neuropati berat> 10. Hasil. Sebanyak 60 subjek memenuhi kriteria inklusi, 48,3% laki-laki dengan usia rata-rata (SD) 20,7 ± 7,6 tahun. Nilai hemoglobin rata-rata 6,7 ± 0,9 g / dL dan serum ferritin darah median (IQR) adalah 2873 (1900-3859) μg / L. Tiga puluh dua subjek memiliki neuropati; 19 (31,7%) neuropati ringan dan 13 (21,6%) neuropati sedang. Simpulan. Insiden neuropati perifer pada pasien dengan talasemia menurut skor TCNS cukup tinggi.
Factors Affecting Mortality in Adult Tetanus Patients Lohghinee Khrisnan; Anam Ong; Ramdan Panigoro
Althea Medical Journal Vol 2, No 2 (2015)
Publisher : Faculty of Medicine Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (510.996 KB)

Abstract

Background: Tetanus is a devastating disease that is associated with high mortality. The aim of this study was to analyze the prognostic factors that were associated with high mortality in tetanus. Methods: This was an analytical study and the data was collected retrospectively from 1 January 2010–31October 2012 in the Department of Neurology, Dr. Hasan Sadikin General Hospital.Results: Out of hundred thirty two cases only 87 cases of tetanus patient have complete medical records. There were 54 male and 33 female. The age range was 35-49 years old. Mortality rate was high (32.18%). The most frequent wound site is extremities (85.07 %). The absence of post-injury tetanus vaccination was 75.86%, period of onset <48 hours was 67.82% and the use of mechanical ventilation and tracheostomy 36.07%. Mortality was significantly associated with an incubation period <7 days (p<0.05), presence of fever(p <0.05), co morbidity of autonomic storm (p<0.01), and the severity of the disease by grade 5 (p<0.05).Conclusions: Factors affecting the mortality in adult tetanus patients were significant for incubation period, fever, co morbidity and severity of the disease by grade. [AMJ.2015;2(1):157–61]
Acute Iron Dextran Injection Increases Liver Weight and Reduces Glycerol Kinase Expression in Liver Ramdan Panigoro; Fadhal M. Ahmad; Uni Gamayani; Neni Anggraeni; Rini Widyastuti; Mohammad Ghozali; Mas Rizky A.A Syamsunarno
Indonesian Journal of Clinical Pharmacy Vol 7, No 4 (2018)
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (319.699 KB) | DOI: 10.15416/ijcp.2018.7.4.236

Abstract

Iron is essential and needed in a very small amount. When iron exceeds normal need, metabolic alteration occurs, causing hepatosteatosis. The mechanism of iron inducing hepatosteatosis remains unclear. Glycerol kinase, the enzyme responsible in triglyceride synthesis initiation, is assumed to have a role in the pathomechanism of hepatosteatosis. This study aimed to investigate the gene expression of glycerol kinase in an acute iron overload condition. This study was conducted in Animal Laboratory Faculty of Medicine and Central Laboratory Universitas Padjadjaran from May to June 2017. Three groups of mice were divided by the dose of iron dextran injection (0, 0.1, 0.3 mg/day/mice). After 19 days, mice were terminated, liver weight was measured and glycerol kinase gene expression in the liver was determined by semi-qualitative PCR. Quantification of PCR result was calculated by ImageJ software. There was a significant change in liver weight of the mice in a dose-dependent manner of iron injection. The expression of glycerol kinase tended to decrease, but statistically insignificant. Acute iron dextran injection increases liver weight and tends to reduce glycerol kinase gene expression in mice liver.Keywords: Glycerol kinase, hepatosteatosis, iron overload Efek Zat Besi Dosis Tinggi Akut dalam Meningkatkan Berat Organ dan Menurunkan Ekspresi Gliserol Kinase HeparAbstrakZat besi merupakan nutrien esensial dan diperlukan dalam jumlah yang sangat kecil. Ketika kadar zat besi melebihi kadar normal dalam tubuh, terjadi perubahan metabolisme yang menyebabkan hepatosteatosis. Mekanisme zat besi dalam menyebabkan hepatosteatosis masih belum diketahui secara pasti. Gliserol kinase, enzim yang menginisiasi sintesis trigliserida, diduga berperan dalam patomekanisme hepatosteatosis. Penelitian ini bertujuan untuk meneliti ekspresi gen gliserol kinase pada hepar pada kondisi tinggi zat besi akut. Penelitian ini dilakukan di Laboratorium Hewan Fakultas Kedokteran dan Laboratorium Sentral Universitas Padjadjaran dari bulan Mei sampai dengan Juni 2017. Tiga kelompok mencit dibagi berdasarkan dosis injeksi iron dextran intraperitoneal (0, 0,1, 0,3 mg/hari/ekor). Setelah 19 hari, mencit diterminasi, berat hepar ditimbang dan ekspresi gen gliserol kinase diukur dengan metode semi-kualitatif PCR. Kuantifikasi hasil PCR dilakukan dengan menggunakan aplikasi ImageJ. Terdapat peningkatan berat hepar secara signifikan yang sejalan dengan dosis ijeksi zat besi. Ekspresi gen gliserol kinase cenderung menurun, meskipun secara statistik tidak signifikan. Keadaan tinggi kadar zat besi yang akut meningkatkan berat hepar dan cenderung menurunkan ekspresi gen gliserol kinase pada hepar mencit.Kata kunci: Gliserol kinase, hepatosteatosis, zat besi berlebih
Co-Authors Adi Imam Cahyadi Ahmad Rizal ahmad rizal Aih Cahyani Al Rasyid Alif Bagus Rakhimullah Alvinsyah Adhityo Pramono Anam Ong Anam Ong Ani Melani Maskoen Asep Nugraha Hermawan Bashari, Muhammad H. Cissy B. Kartasasmita Cissy B. Kartasasmita Dewanto, Julius B Diah Dhianawaty Dias Aryani Dwi Agustian Dwi Agustian Edhyana Sahiratmadja Edhyana Sahiratmadja Edhyana Sahiratmadja Elsa Pudji Setiawati Erick Khristian Fadhal M. Ahmad Fanny Adhy Putri Haryono Tansah, Haryono Heda M. Duddy Heda Melinda Duddy Helena Napitu, Helena Herman Susanto Ida Parwati Ina Setiowati Ina Setiowati Irianto, Gunawan Jeri Nobia Purnama Julius B Dewanto Julius Broto Dewanto, Julius Broto Kevin Dominique Tjandraprawira Khristian, Erick Lailiyya, Nushrotul Lelani Reniarti Lisda Amalia Lohghinee Khrisnan Lohghinee Khrisnan, Lohghinee Mas Rizky A.A Syamsunarno Mas Rizky A.A. Syamsunarno Masahiko Kurabayasi Mohammad Ghozali Mohammad Ghozali, Mohammad Mugia Bayu Rahardja Mugia Bayu Raharja Muhammad, Rifqi Y Neni Anggraeni Novi Vicahyani Utami Nur Atiik Nurul Setia Rahayu Nushrotul Lailiyya Pandji Irani Fianza Penggoam, Simeon Pramono, Alvinsyah Adhityo Pricilla Purushothman Purnama, Jeri N. Putri, Fanny Adhy Ratu Safitri Ratu Safitri Ratu Safitri Reni Ghrahani Rifqi Y Muhammad Rini Widyastuti Samsudin Surialaga Sari Puspa Dewi Shenny Dianathasari Santoso Simeon Penggoam Sunaryati Sudigdoadi Sunaryati Sudigdoadi Surbakti, Indra Murty Tatsuya Iso Tri H. Ahmad Tri Hanggono Achmad Tri Hanggono Ahmad Uni Gamayani Uni Gamayani Uni Gamayani Uni Gamayani Uni Gamayani Uni Gamayani, Uni Wardoyo, Chandra Calista Yudi Mulyana Hidayat Yusuf Wibisono Yusuf Wibisono