Article Per Year (5 Year)
p-Index From 2020 - 2025
0.882
P-Index
This Author published in this journals
All Journal Jurnal Biosains Pascasarjana Jurnal Ilmu Kesehatan Bhakti Husada: Health Sciences Journal Anakes: Jurnal Ilmiah Analis Kesehatan Narra J Jurnal Kesehatan Kartika
Khristian, Erick
Unknown Affiliation
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Dentistry Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Veterinary

Published : 5 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 5 Documents
Search

 1 
PENGGUNAAN KUTEKS SEBAGAI ALTERNATIF MOUNTING MEDIA PADA PEMBUATAN PREPARAT JARINGAN Khristian, Erick; Purnama, Jeri Nobia
JURNAL KESEHATAN KARTIKA Vol. 15 No. 2 (2020): Jurnal Kesehatan Kartika
Publisher : Faculty of Health Science and Technology, University of Jenderal Achmad Yani

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26874/jkkes.v15i2.151

Abstract

Mounting media is one of the important components in making tissue slides. Mounting media serves to cover the tissue on the slide to protect it from damage and also creates a refractive index between the tissue and objective lens approach, so that it can be seen clearly when observed using a microscope. Mounting media commonly used in the laboratory for tissue slide include entelan, Canada balsam and DFX. The aim of this research is to find an alternative mounting media that is cheap, easy to obtain and has a value comparable to the commonly used mounting media. The study used various organs of the liver, kidney, testes and brain stained with Haematoxylin Eosin. The analysis used is descriptive analysis by comparing the quality of the tissue slides using different mounting media, that is entelan and nail polish. The test parameters to see the quality of the results from the use of mounting media are the contrast of the tissue slides, the details of the nucleus and the brightness of the cytoplasm. The results of the study showed comparable values ​​for tissue slide contrast, nucleus detail and the brightness of the cytoplasm for all the organs made. It is advisable to use other stains to ensure that the use of nail polish can be used as a mounting media in the laboratory
HISTOPATHOLOGICAL STRUCTURE OF THE RAT KIDNEY AFTER ADMINISTRATION OF SAPPAN WOOD EXTRACT (Caesalpinia sappan L.) IN IRON OVERLOAD CONDITION Jeri Nobia Purnama; Firdawati, Nurul; Khristian, Erick; Safitri, Ratu; Utama, Gemilang Lara; Fakhira, Anisa Muthia
Jurnal Biosains Pascasarjana Vol. 26 No. 2 (2024): JURNAL BIOSAINS PASCASARJANA
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbp.v26i2.2024.87-98

Abstract

Excessive amounts of iron in the body can lead to damage to various organs, including the kidneys. Iron chelators have been demonstrated to effectively reduce the accumulation of this excess iron. This study aims to investigate the impact of administering sappan wood extract (Caesalpinia sappan) as an adjuvant to iron chelators on the structure and function of kidneys in a rat model of iron overload. The experimental research, spanning 28 days, employed a Completely Randomized Design (CRD) involving 30 male Wistar rats (Rattus norvegicus) distributed across 6 test groups. Iron dextran at 60 mg/kg bb induced iron overload, while a comparative iron chelator, deferiprone, was given at 1.35 mg/kg bb. Various doses of Sappan wood extract (SWE) 100, 150, and 200 mg/kg bb were administered to separate test groups. The parameters that are being observed include the distribution of iron and the structure of kidney injury. At a 95% confidence level, the acquired data were examined using analysis of variance (ANOVA), and the Duncan test was used to see whether there were any differences. The study's findings demonstrated that each treatment group's kidney iron levels differed from the excess iron-containing control group (p <0.05). The histological investigation results demonstrated a significant difference in damage scores (p <0.05) between the groups administered secang wood extract and the excess besei control group. Adminstered of 100 mg/kgbw dose of EKS might lower their organ iron levels and lessen the harm that the iron did to their kidneys. Keyword: Sappan Wood (Caesalpinia sappan L.), Iron Overload, Kidney
Pengaruh frekuensi konsumsi kopi robusta (coffea canephora) terhadap gambaran histopatologi & biokimia ginjal pada tikus putih jantan galur wistar Suharjiman, Suharjiman; Khristian, Erick; Supriadi, Dedi; Purnama, Jeri Nobia; Budiana, Teguh Akbar
Jurnal Ilmu Kesehatan Bhakti Husada: Health Sciences Journal Vol. 15 No. 02 (2024): Jurnal Ilmu Kesehatan Bhakti Husada: Health Science Journal
Publisher : Lembaga Penelitian Sekolah Tinggi Ilmu Kesehatan Kuningan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34305/jikbh.v15i02.1214

Abstract

Latar Belakang: Kopi merupakan minuman yang umum dikonsumsi dan memiliki kemampuan sebagai antioksidan yang dapat memberikan manfaat terhadap kesehatan ginjal. Tujuan dari penelitian untuk melihat efek frekuensi konsumsi kopi robusta terhadap gambaran histopatologis dan biokimia organ ginjal meliputi kadar ureum dan kreatinin pada model hewan uji tikus galur wistar.Metode: Penelitian ini bersifat eksperimental dengan desain Posttest Only Group Control. Tikus dibagi secara acak menjadi 4 kelompok perlakuan (masing-masing terdiri dari 5 ekor). Perlakuan per oral ekstrak kopi diberikan selama 30 hari untuk dievaluasi parameter histopatologis dan fungsi ginjal.Hasil: Hasil penelitian menunjukkan kadar ureum dan kreatinin serum tidak ada perbedaan yang signifikan pada setiap peningkatan pemberian dosis kopi robusta setelah 30 hari masa penelitian. Skoring histopatologis ginjal mencit tidak menunjukan perbedaan yang signifikan pada kelompok yang diberikan kopi robusta terhadap kelompok kontrol (p value < 0,005).Kesimpulan: Penelitian ini menyimpulkan bahwa konsumsi kopi Robusta tidak memberikan efek negatif terhadap fungsi dan struktur histopatologis ginjal mencit. Temuan tersebut melengkapi pengetahuan tentang manfaat dari konsumsi kopi yang tidak berdampak pada kesehatan ginjal.
Intravenous administration of iron dextran as a potential inducer for hemochromatosis: Development of an iron overload animal model Khristian, Erick; Ghozali, Mohammad; Bashari, Muhammad H.; Purnama, Jeri N.; Irianto, Gunawan; Panigoro, Ramdan; Safitri, Ratu
Narra J Vol. 4 No. 3 (2024): December 2024
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narra.v4i3.1003

Abstract

Iron overload in transfusion-dependent thalassemia patients represents a significant public health challenge due to its high mortality rate and risks of severe complications. Therefore, developing safe and effective therapeutic modalities for managing iron overload is critical, as current animal models inadequately replicate human conditions. The aim of this study was to investigate the effects of intravenous iron dextran on hepatocyte morphology, liver iron concentration, and serum iron profile changes as a model for hemochromatosis. An experimental design with a post-test-only control group method was conducted using animal models. Fifty rats were used and divided into ten groups, nine received different intravenous doses of iron dextran: 10, 20, 30, 40, 50, 60, 80, 100, and 120 mg/kg body weight (BW) and a control group received no treatment. The results showed that intravenous iron dextran starting at a dose of 10 mg/kg BW caused significant changes in liver iron concentration while starting at 20 mg/kg BW significantly affected hepatocyte morphology, transferrin levels, unsaturated iron binding capacity, serum iron levels, and transferrin saturation. Intravenous iron dextran starting at 40 mg/kg BW resulted significant changes in the level of total iron binding capacity compared to control group. In conclusion, intravenous iron dextran significantly altered hepatocyte morphology, increased liver iron concentration, and modified the serum iron profile, reflecting changes that might be observed in patients with transfusion-dependent thalassemia.
Pengembangan Metode Identifikasi Kadar Ion Besi Sampel Urin Berbasis Separasi Magnetik Permana, Ellsie Viendra; Khristian, Erick; Oktaviani, Nidya; Muhammad Rizaldi Ridwan; Siti, Salma; Maharani, Devi
Anakes : Jurnal Ilmiah Analis Kesehatan Vol. 10 No. 2 (2024): ANAKES: Jurnal Ilmiah Analis Kesehatan
Publisher : Universitas Mohammad Husni Thamrin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37012/anakes.v10i2.2457

Abstract

Kelebihan zat besi (iron overload) dapat disebabkan oleh berbagai faktor, termasuk gangguan genetik seperti hemokromatosis herediter dan penyebab sekunder seperti transfusi darah berulang atau asupan besi yang berlebihan, yang sering terjadi pada pasien thalasemia. Penelitian ini bertujuan untuk mengembangkan metode baru dalam menentukan kadar ion besi dalam urin. Beberapa senyawa pembentuk presipitat ion besi yang diidentifikasi meliputi sulfit, peroksida, dan hidroksida, dengan hasil optimum diperoleh dari presipitasi menggunakan hidroksida berdasarkan parameter kestabilan dan kecepatan pengendapan dengan magnet. Sebelum analisis, sampel urin diperlakukan dengan penambahan magnesium untuk menghindari gangguan dari presipitasi ion fosfat. Hasil pengendapan optimum menggunakan hidroksida dicapai pada menit ketiga untuk ion besi dengan kadar 0,5 – 2,5 g/L. Nilai absorbansi turbiditas awal pada menit pertama berkisar antara 0,336 hingga 0,44, sedangkan absorbansi maksimum saat presipitasi maksimum berkisar antara 0,393 hingga 0,806, meningkat seiring dengan kenaikan konsentrasi Fe²⁺ dalam larutan. Setelah penarikan magnet dengan palladium ke dasar kuvet, absorbansi menurun dan stabil sejak menit kesembilan dengan rentang absorbansi 0,047 – 0,173. Absorbansi mencapai titik terendah pada menit keempat belas dengan rentang 0,002 – 0,03. Kurva standar yang dihasilkan dari selisih absorbansi turbiditas presipitat dan absorbansi supernatant setelah magnetisasi menunjukkan linearitas sebesar 0,94.
Co-Authors Bashari, Muhammad H. Dedi Supriadi Fakhira, Anisa Muthia Firdawati, Nurul Gemilang Lara Utama Irianto, Gunawan Jeri Nobia Purnama Maharani, Devi Mohammad Ghozali, Mohammad Muhammad Rizaldi Ridwan Oktaviani, Nidya Permana, Ellsie Viendra Purnama, Jeri N. Purnama, Jeri Nobia Ramdan Panigoro Ratu Safitri Siti, Salma Suharjiman, Suharjiman Teguh Akbar Budiana