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All Journal Jurnal Sain Veteriner Buletin Peternakan Proceeding Buffalo International Conference Jurnal Ilmu Ternak Veteriner JITRO (Jurnal Ilmiah dan Teknologi Peternakan Tropis) Jurnal Ternak

Syahruddin Said

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Author-ID : 368575

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Veterinary Other

Published : 9 Documents Claim Missing Document

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Successful ICSI depends on DNA stability and disulfide status of sperm nuclei Said, Syahruddin; Niwa, Koji
Indonesian Journal of Animal and Veterinary Sciences Vol 9, No 3 (2004)
Publisher : Indonesian Animal Sciences Society

Relation of successful ICSI (intracytoplasmic sperm injection) with DNA stability and thiol-disulfide status of rat spermatozoa nuclei was investigated. Testicular, caput and cauda epididymal spermatozoa have been used for this research. The nuclei of these spermatozoa were stained with aeridine orange for DNA stability test and labeled with mBBr for thiol-disulfide status before injesting into oocytes. Cauda epididymal spermatozoa were very stable and contained high disulfide than caput epididymal and testicular spermatozoa. Results from spermatozoa injection into oocytes show that they were capable to fertilize oocytes. However, testicular spermatozoa can not develop up to blastocyst stage. These results indicated that successful ICSI at least in the rat depends on DNA stability and disulfide status of sperm nuclei. Â Key words: ICSI, DNA stability, disulfide status, acridine orange, mBBr

Rescuing genetic material of unexpectedly die animal Said, Syahruddin; Saili, Takdir
Indonesian Journal of Animal and Veterinary Sciences Vol 12, No 2 (2007)
Publisher : Indonesian Animal Sciences Society

Rat cauda epididymides were kept in 1.5-ml centrifuge tubes containing 1 ml milli-Q water or physiological saline (0.9% NaCl) and stored and freezed at -196ÂºC without cryoprotectant for up to 7 days. After thawing of the cauda epididymis, all spermatozoa were non-motile immediately after collection. All oocytes injected with sperm heads (nuclei) of spermatozoa collected from frozen-thawed cauda epididymis in saline were activated (100%) and gradually decreased (P<0.05) in cauda epididymis frozen in milli-Q water at -196Â°C (86%), and in control (69%). In activated oocytes, a large proportion of sperm heads had transformed into male pronuclear formation (66-78%). When 1-cell embryos were cultured for 120 h, 7% developed to blastocyst stages. These results indicate that genetic materials of species (at least in the rat) that had unexpectedly die can be saved by a simple method. Key words: ICSI, Sperm Heads, Piezo-Injection, Frozen, Rat

Rescuing genetic material of unexpectedly die animal Syahruddin Said; Takdir Saili
Jurnal Ilmu Ternak dan Veteriner Vol 12, No 2 (2007): JUNE 2007
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Rat cauda epididymides were kept in 1.5-ml centrifuge tubes containing 1 ml milli-Q water or physiological saline (0.9% NaCl) and stored and freezed at -196ºC without cryoprotectant for up to 7 days. After thawing of the cauda epididymis, all spermatozoa were non-motile immediately after collection. All oocytes injected with sperm heads (nuclei) of spermatozoa collected from frozen-thawed cauda epididymis in saline were activated (100%) and gradually decreased (P<0.05) in cauda epididymis frozen in milli-Q water at -196°C (86%), and in control (69%). In activated oocytes, a large proportion of sperm heads had transformed into male pronuclear formation (66-78%). When 1-cell embryos were cultured for 120 h, 7% developed to blastocyst stages. These results indicate that genetic materials of species (at least in the rat) that had unexpectedly die can be saved by a simple method. Key words: ICSI, Sperm Heads, Piezo-Injection, Frozen, Rat

Successful ICSI depends on DNA stability and disulfide status of sperm nuclei Syahruddin Said; Koji Niwa
Jurnal Ilmu Ternak dan Veteriner Vol 9, No 3 (2004): SEPTEMBER 2004
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Relation of successful ICSI (intracytoplasmic sperm injection) with DNA stability and thiol-disulfide status of rat spermatozoa nuclei was investigated. Testicular, caput and cauda epididymal spermatozoa have been used for this research. The nuclei of these spermatozoa were stained with aeridine orange for DNA stability test and labeled with mBBr for thiol-disulfide status before injesting into oocytes. Cauda epididymal spermatozoa were very stable and contained high disulfide than caput epididymal and testicular spermatozoa. Results from spermatozoa injection into oocytes show that they were capable to fertilize oocytes. However, testicular spermatozoa can not develop up to blastocyst stage. These results indicated that successful ICSI at least in the rat depends on DNA stability and disulfide status of sperm nuclei. Key words: ICSI, DNA stability, disulfide status, acridine orange, mBBr

Co-Authors ACHMAD FARAJALLAH Aries Boediono Dinda Ayu Permata Sari Dyah Perwitasari Febyastri, Meda Ferdy Saputra Hermansyah, Alfandy Kaiin, Ekayanti Mulyawati Koji Niwa Kuswati Kuswati M Agus Setiadi Moch. Syamsul Arifin Zein, Moch. Syamsul Arifin MOZES R. TOELIHERE Nahrowi Nanditya, Wisnu Krisna Paskah Partogi Agung Pintaka Bayu Putra, Widya Risqa Novita, Risqa Rudy Priyanto Srihadi Agungpriyono TAKDIR SAILI Takdir Syahruddin Said Tri Eko Susilorini

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Journal : Jurnal Ilmu Ternak Veteriner