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All Journal HAYATI Journal of Biosciences Prosiding Seminar Nasional MIPA Biotika: Jurnal Ilmiah Biologi BIOTROPIA - The Southeast Asian Journal of Tropical Biology AL KAUNIYAH Biogenesis: Jurnal Ilmiah Biologi AGRIVITA, Journal of Agricultural Science Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (Journal of Natural Resources and Environmental Management) JURNAL ISTEK Journal of Tropical Biodiversity and Biotechnology Pro-Life REINWARDTIA BERITA BIOLOGI JURNAL BIOLOGI INDONESIA BIOEDUSCIENCE Jurnal Biologi Tropis Bioma : Jurnal Biologi Indonesia Makara Journal of Science Reinwardtia Berita Biologi
Andi Salamah
Department Of Biology, Faculty Of Mathematic And Natural Sciences, Universitas Indonesia, Depok 16424, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Earth & Planetary Sciences Education Environmental Science Immunology & microbiology Materials Science & Nanotechnology Veterinary

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Optimizing DNA Extraction Methods from Leaf and Wood Tissues to Support Dipterocarp Conservation and Sustainable Forest Management Arrofaha, Nawwall; Rachmat, Henti Hendalastuti; Dwiyanti, Fifi Gus; Adinugroho, Wahyu Catur; Siregar, Iskandar Zulkarnaen; Kamal, Irsyad; Syaputra, Dhika; Salamah, Andi
Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (Journal of Natural Resources and Environmental Management) Vol 16 No 1 (2026): Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (JPSL)
Publisher : Pusat Penelitian Lingkungan Hidup, IPB (PPLH-IPB) dan Program Studi Pengelolaan Sumberdaya Alam dan Lingkungan, IPB (PS. PSL, SPs. IPB)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jpsl.16.1.13

Abstract

Dipterocarpaceae are economically important, contributing over 85% of Indonesia's timber exports. However, this crucial resource is increasingly threatened by illegal logging, habitat destruction, and the illegal timber trade, which jeopardize dipterocarp population. Furthermore, conservation efforts utilizing genetic and forensic techniques often encounter substantial challenges due to the complexities in DNA extraction protocol. To address this, the study aimed to enhance the efficiency of DNA extraction methodologies by comparing two methods: the modified cetyltrimethylammonium bromide (CTAB) and the Genomic DNA Mini Kit (Plant) from Geneaid Biotech Ltd. The research focused on leaf and wood samples from two species, spesifically Rubroshorea leprosula (Miq.) P.S.Ashton & J.Heck and Shorea laevis Ridl. For each of these species, five leaf and five wood samples were extracted using both methods. The quality of the DNA extraction was evaluated using electrophoresis and quantified with a Qubit fluorometer. Higher DNA concentrations were obtained with the modified CTAB method compared to the GeneAid kit for both R. leprosula and S. laevis, particularly in leaf tissue. The GeneAid kit consistently exhibited low DNA yield efficiency compared to the modified CTAB method for both species. Additionally, PCR amplification of both leaf and wood samples confirmed that the extracted DNA was suitable formolecular analyses. These findings not only contributed to laboratory applications but also served as practical tools for species identification and genetic conservation for sustainable forest management and law enforcement.
Co-Authors Abinawanto Abinawanto Achmad Dinoto Adinugroho, Wahyu Catur Agus Haryadi Anggita R Hafsari Anna Widyastuti Ariyanti Oetari Arrofaha, Nawwall Asep Sadili Asep Sadili Asep Sadili, Asep Atria, Mega Az Zahra, Najwa Wafiyah Bayu Adjie Brearley, Francis Q Citra Karina Windarti Decky Indrawan Junaedi Decky Indrawan Junaedi Dee Dee Al Farishy Dian Hendrayanti Dika Migi Priyono Dwiranti, Astari Edi Mirmanto Edi Mirmanto Edi Mirmanto, Edi Edi Mirtanto Endang Sukara Fadilah, Nurrahmi Fifi Gus Dwiyanti Firdha Meidi Irshanty Francis Q Brearly Iriani, Nur Annisa Irshanty, Firdha Meidi Irsyad Kamal ISKANDAR ZULKARNAEN SIREGAR Isnaenisa Rachma Khoiriyah, Istatik Kusumadewi Sri Yulita Kuswata Kartawinata KUSWATA KARTAWINATA Kuswata Kartawinata Lulut Dwi Sulistyaningsih Mansur Halik Marlina Ardiyani Maryanto, Andi Eko Mouleidi Dwi Putri Muhammad Mansur MUHAMMAD MANSUR Mutia Syadewi Najwa Wafiyah Az Zahra Niarsi Merry Hemelda Niarsi Merry Hemelda Niarsi Merry Hemelda NUNIK SRI ARIYANTI Nur Laili Pipit Marianingsih Pipit Marianingsih Pipit Marianingsih Priyono, Dika Migi Purba, Eliza Pricilia Puspitaningrum, Hardini Putrika, Afiatry Rachmat, Henti Hendalastuti Rahmadina, Amara Rahmandika, Ghifari Ririn Oktarina Saifudin Saifudin Sarah Tsabituddinillah Sarjiya Antonius Siska Apriliani Sulistiani Sulistiani, Sulistiani Syaputra, Dhika Tri Joko Santoso Tri Joko Santoso TRI JOKO SANTOSO Tri Muji Ermayanti Tsabitudinillah, Sarah Wellyzar Sjamsuridzal WIBOWO MANGUNWARDOYO Winarni, Nurul Laksmi Windri Handayani Windri Handayani Windri Handayani Wita Wardani Wita Wardani Yuki Ichinose Yuki Ichinose Yuniati, Ratna