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All Journal ACTA VETERINARIA INDONESIANA Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Jurnal Peternakan Indonesia Animal Production : Indonesian Journal of Animal Production Jurnal Nukleus Peternakan Jurnal Ilmiah AgriSains ANGON: Journal of Animal Science and Technology Jurnal Veteriner
Dadang Mulyadi Saleh
Jurusan Peternakan, Bagian Fisiologi dan Reproduksi Ternak Terapan, Fakultas Peternakan, Universitas Jenderal Soedirman
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Environmental Science Immunology & microbiology Medicine & Pharmacology Veterinary Other

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Quality of Native Chicken Spermatozoa in Different Diluents Supplementing with Vitamin C and Their Ability to Fertilise Jatra, Miftah Laili Dwi; Ismoyowati; Saleh, Dadang Mulyadi; Tugiyanti, Elly; Sumaryadi, Mas Yedi; Yunitasari, Fitri
Jurnal Ilmu-Ilmu Peternakan Vol. 35 No. 3 (2025): December 2025
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiip.2025.035.03.8

Abstract

This study aimed to examine the effects of the interaction of the addition of vitamin C, the type of diluent, and the duration of storage at 5°C on the quality and fertility of Rooster Kampung spermatozoa and the fertile period of Red Kedu hen eggs. Semen was collected from 10 roosters subjected to vitamin C level treatment (0 mg, 0.1 mg, 0.2 mg, or 0.3 mg), two diluent types (lactated Ringer (RL) and skim milk + 50 mM glucose), and storage durations (0 hours, 3 hours, 6 hours, or 9 hours). The sperm samples meeting the quality standards were inseminated into 64 Red Kedu hens. Phase 1 of the study used a factorial randomised design (2´4´4) followed by variance analysis and orthogonal polynomial testing. Phase 2 applied the eight best result treatments from phase 1 via a completely randomised design with Tukey’s test for post hoc analysis. The results revealed a highly significant interaction effect (P<0,01) between vitamin C level and diluent type on motility, plasma membrane integrity (IPM), and sperm abnormality. The interaction effect of vitamin C level and storage duration had a very significant effect (P<0.01) on motility, IPM, and viability. The interaction effect of diluent type and storage duration significantly affected (P<0.01) motility and IPM. However, no significant effect (P>0.05) among all three variables was observed on fertility or the fertile period. In summary, the addition of 0.3 mg of vitamin C, lactated Ringer’s diluent, and 6 hours of storage at 5°C maintained sperm quality and resulted in 51.78±37.64 fertility and a fertile period of 15 days in Red Kedu hens.
Kualitas Spermatozoa Y Hasil Separasi Seks dengan Medium Bovine Serum Albumin (BSA) dan Lama Inkubasi yang Berbeda pada Domba Sakub: Kualitas Spermatozoa Y Hasil Separasi Seks dengan Medium Bovine Serum Albumin (BSA) dan Lama Inkubasi yang Berbeda pada Domba Sakub Imam Prasetyo, Setiadi; Anis, Ahmad Dzulfahmi; Sumaryadi, Mas Yedi; Saleh, Dadang Mulyadi
Jurnal Peternakan Indonesia (Indonesian Journal of Animal Science) Vol 28 No 1 (2026): Jurnal Peternakan Indonesia
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jpi.28.1.11-22.2026

Abstract

Penelitian ini bertujuan untuk mengetahui pengaruh medium Bovine Serum Albumin (BSA) dan lama inkubasi yang berbeda terhadap kualitas spermatozoa Y domba Sakub. Penelitian dirancang menggunakan pola factorial 2x3 dengan rancangan dasar acak kelompok (RAK). Faktor I gradien BSA (G) terdiri dari dua tingat konsentrasi dua level (g1=4:8% dan g2=5:10%) dan faktor II lama inkubasi (T) terdiri dari level (t1=30, t2=40, dan t3=60 menit). Variabel yang diamati yaitu motilitas, abnormalitas, konsentrasi dan viabilitas spermatozoa Y. Hasil analisis statistik menunjukkan bahwa tidak ada interaksi antara gradien BSA dan lama inkubasi (P>0,05) terhadap kualitas spermatozoa. Namun gradien BSA berpengaruh sangat nyata (P<0,01) dan lama inkubasi berpengaruh nyata (P<0,05) terhadap kualitas spermatozoa Y domba Sakub. Nilai motilitas tertinggi pada t1 (73,67 ± 1,41%), abnormalitas terendah pada t1 (9,92 ± 1,06%), konsentrasi tertinggi pada g2 (1196,67 x 106), dan viabilitas tertinggi pada t1 (73,5 ± 1,00 %). Disimpulkan bahwa untuk memperoleh kualitas spermatozoa Y yang tinggi sebaiknya digunakan medium BSA dengan konsentrasi 5-10% (g2) dan lama inkubasi 30 menit (t1) dan masih memenuhi standar untuk pelaksanaan inseminasi Buatan (IB).
The Effect of Egg-White Gradient and Incubation Time on Sex Separation for Semen Quality in Dorper Rams Alwan, Mochamad Daffa; Sumaryadi, Mas Yedi; Saleh, Dadang Mulyadi; Setyaningrum, Agustinah; Rahayu, Sri
Jurnal Veteriner Vol. 27 No. 1 (2026)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/

Abstract

Improving Dorper sheep meat production efficiency through sperm sex separation (sexing) technology using egg white gradients is an economical alternative, yet it is often hindered by the decline in post-freezing semen quality due to cold shock. This study was aimed to evaluate the effectiveness of combining egg white medium concentration gradients and incubation times on the quality of liquid and frozen-thawed semen. Using fresh semen from two Dorper rams (aged 24 months), this study employed a 2x3 Factorial Randomized Block Design (RBD) with four replications. Treatments consisted of two gradient levels (15:30% and 20:35%) and three incubation times (30, 45 and 60 minutes). The obtaimed data were analyzed using Analysis of variane and continued with Duncan's multiple range test if there were significantly differences betrweem treatments. Results showed that incubation time significantly affected (P<0.05) motility, viability and concentration. Specifically, the 15:30% gradient with 45 minutes of incubation proved to be the most optimal, yielding 51.25% post-sexing motility and 53.88% viability. This superiority persisted through the post-thawing stage, where this treatment recorded the highest motility (12.50%) and viability (14.25%). However, frozen semen values were generally low. Conversely, 60-minute incubation significantly reduced quality due to metabolic exhaustion, while the 20:35% gradient had excessive viscosity that hindered sperm penetration, drastically lowering the final concentration to 4.75 x 10⁷ cells/mL. Although fluctuations occurred, abnormality rates across all treatments remained below the 20% threshold. It is concluded that freezing techniques require evaluation, using a 15:30% egg white gradient with 45 minutes of incubation at 37°C is the best protocol in this study recommended for Dorper sheep sex separation, as it maximally maintains membrane integrity and sperm survival in both liquid and frozen phases.
Co-Authors Abdurrahman, Muadz Afri Wahyuningsih Agustinah Setyaningrum, Agustinah Alwan, Mochamad Daffa Andara, Ghaitza Anis, Ahmad Dzulfahmi Aras Prasetiyo Nugroho Aras Prasetiyo Nugroho, Aras Prasetiyo Ardiansyah, Muhammad Arfan Nur Ariefin Ade Prasetyo Armelia, Vony Armelia, Vony Asep Nasirin Awaludin, Khairul Burhanudin, Deden Deby Tripriliawan Elly Tugiyanti Ferdian Nugroho Gagah Hendra Wijaya, Gagah Hendra Hidayah, Chomsiatun Nurul Hidayat, Nu'man Hidayat, Nu’man Imam Prasetyo, Setiadi Imbang Haryoko Ismoyowati Ismoyowati, Tri Wahyuni Jatra, Miftah Laili Dwi Juhri, Mohamad Aminudin M. Y. Sumaryadi Maidaswar Maidaswar Mangliari, Yugys Novie Andri Setianto Nurrofingah, Ulfa Nursabani, Agung Mohamad Paulus Suparman Permana, Fachmi Angga Rilandana, Dipta Santoso, Ilham Budi Septi Dwi Lestari Septiyani, Yunda Dwi Sigit Mugiyono Sri Lestari SRI RAHAYU Sugiyatno Sugiyatno Sukardi Sukardi Sutimah Baktiningsih Taswin Rahman Tagama Tugistan, Laudza Rechttiana Vian Graha Wijaya Widodo, Hermawan Setyo Yuniar, Tiara Utami Yunitasari, Fitri