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Effect of Water Availability on the Growth and Secondary Metabolites of Zodia (Evodia suaveolens) Andriyana Setyawati; Edi Purwanto; Ahmad Yunus; Samanhudi Samanhudi; Amalia Tetrani Sakya; Muji Rahayu; Djoko Purnomo; Retna Bandriyati Arniputri; Gani Cahyo; Qonita Rahma Dwiyanti; Saat Egra; Okky Talitha; Alfida Muthi’ah
Agrosains : Jurnal Penelitian Agronomi Vol 25, No 2 (2023): Agrosains: Jurnal Penelitian Agronomi
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/agsjpa.v25i2.81528

Abstract

Zodia are included in medicinal plants because it contains secondary metabolites such as flavonoids, tannins, alkaloids, steroids/triterpenoids that have many benefit and also as mosquito repellents. The growth and content of secondary metabolites in plants can be affected by environmental factors such as water availability. Research on the cultivation of this crop is still limited. Environmental modification in cultivation is needed to increase its secondary metabolites, and the study of the potential of this plant as medicine are necessary. This study aimed to determine the effect of water availability on the growth and secondary metabolites of zodia. The research was carried out in July-November 2021 in Greenhouse, Faculty of Agriculture, Universitas Sebelas Maret, Surakarta. The method used was a completely randomized design, one treatment factor with 4 levels namely, 100, 80, 60 and 40% of field capacity. Each treatment was repeated 6 times so that there were 24 experimental polybags. The data analysis used is Analysis of Variance, and the Duncan’s Multiple Range Test at the 5% level. The results showed that the availability of water at a level of 60% field capacity gave the best average growth parameters (plant height and number of leaves) among other treatments. The flavonoid content in each treatment showed the same average. The results of the GCMS (Gas Cromatography and Mass Spectroscopy) test of zodia leaves showed that there were dominant bioactive compounds, namely ethylbenzene, octadecamethyl cyclononasiloxane, pentadecanoid acid and heptadecanoid acid. It is suspected that the content of these compounds has the potential as antifungal, antibacterial, antioxidant and anticancer.
Budidaya Anggur dengan Sistem Pemantauan Berbasis IOT pada Kelompok Wanita Tani “Sekar Arum” Desa Genengan, Kecamatan Jumantono, Kabupaten Karanganyar Fitria Roviqowati; Ahmad Yunus; Edi Purwanto; Retna Bandriyati Arniputri; Samanhudi Samanhudi; Muji Rahayu; Gani Cahyo Handoyo; Andriyana Setyawati; Iswahyudi Iswahyudi; Joko Prihanto
SEMAR (Jurnal Ilmu Pengetahuan, Teknologi, dan Seni bagi Masyarakat) Vol 15, No 1 (2026): Mei
Publisher : LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/semar.v15i1.109864

Abstract

Desa Genengan memiliki potensi geografis yang mendukung budidaya anggur, namun pengembangannya oleh Kelompok Wanita Tani (KWT) “Sekar Arum” terkendala oleh keterbatasan pengetahuan teknik budidaya modern dan sistem pemantauan lingkungan yang masih konvensional. Program pengabdian ini bertujuan untuk: (1) meningkatkan pengetahuan dan keterampilan anggota KWT mengenai budidaya anggur dan teknik grafting, (2) mengimplementasikan sistem pemantauan lingkungan berbasis Internet of Things (IoT), dan (3) menganalisis efektivitas pelatihan serta kendala adopsi teknologi. Metode pelaksanaan meliputi pelatihan teoritis dan praktik, implementasi demplot, serta pemasangan perangkat IoT yang memantau suhu, kelembaban tanah, intensitas cahaya, dan pH. Evaluasi menggunakan pretest-posttest dan kuesioner kepuasan. Hasil menunjukkan partisipasi 22 anggota (100%). Terjadi peningkatan pengetahuan yang signifikan dengan nilai rata-rata pretest 71,03 menjadi posttest 93,05 (gain 22,02 poin). Tingkat keberhasilan praktek grafting mencapai 75%. Berdasarkan angket yang telah dibagian diperoleh data bahwa keseluruhan peserta menyatakan materi yang disampaikan bermanfaat. Tingkat ketertarikan pada kegiatan 72,2% menyatakan sangat menarik serta 22,8% menyatakan menarik. Keseluruhan peserta menyatakan tertarik untuk melakukan budidaya anggur di rumah. Sistem IoT beroperasi dengan baik, meski mengalami kendala konektivitas internet. Analisis mengungkap penerimaan yang lebih tinggi terhadap materi budidaya konvensional dibandingkan teknologi IoT, serta ketergantungan pada infrastruktur digital sebagai penghambat utama. Program ini berhasil meningkatkan kapasitas teknis kelompok dan membuka jalan menuju pertanian presisi. Keberlanjutan memerlukan pendampingan lanjutan, penguatan aspek bisnis, dan solusi infrastruktur komunikasi yang adaptif.Kata kunci: budidaya anggur; IoT; kelompok wanita tani; pertanian presisi; pemberdayaan masyarakat
Application of Livestock Waste Liquid Fertilizer and Mychorriza Biofertilizer on The Growth of Biduri (Calotropis gigantea) Muhammad Naufal Adani; Samanhudi Samanhudi; Amalia Tetrani Sakya; Sri Hartati; Muji Rahayu; Anne Sukmawati
Journal of Biodiversity and Biotechnology Vol 2, No 2 (2022)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v2i2.63262

Abstract

Biduri (Calotropis gigantea) was a plant with several benefits, namely as a medicinal plant and textile material. Biduri was a wild plant that could grow in various types of soil conditions. The existence of biduri was still not fully utilized by many people. So it was necessary to develop the cultivation technology of Biduri to take advantage of this plant. This research was conducted at the field laboratory of Jumantono, Faculty of Agriculture, Universitas Sebelas Maret, Karanganyar, and used a factorial Randomized Complete Block Design (RCBD), which consisted of 2 factors, namely organic liquid fertilizer from livestock waste and mycorrhizae. Livestock waste liquid fertilizer consists of 40ml/kg soil cow urine, 40ml/kg soil goat urine, 40ml/kg soil rabbit urine, and 40ml/kg soil fish waste. Mycorrhizae consisted of 0 grams/plant, 5 grams/plant, 10gram/plant, and 15gram/plant. There were 20 treatment combinations repeated 3 times, so the total was 60 treatment combinations. The interaction between livestock waste liquid fertilizer and mycorrhizae did not significantly affect all the physiological observation variables. The results showed that the application of livestock waste liquid fertilizer had no significant effect on all observations of physiological variables. Giving mycorrhizae shows that it can increase the number of stomata and the width of stomata aperture.
Morphological Character of The Natural Orchid Phalaenopsis Spp. Results of Colchicine Polyploidy Induction Sri Hartati; Samanhudi Samanhudi; Endang Suprapti
Journal of Biodiversity and Biotechnology Vol 4, No 2 (2024)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v4i2.108518

Abstract

Induction of polyploidy in orchids can cause morphological changes such as larger leaf and flower sizes, longer bloom durations, and sharper flower colors. Morphological characterization needs to be done to determine the morphological changes that occur after colchicine treatment. This study aims to determine the morphological characters of natural orchids Phalaenopsis spp. polyploidy induction using colchicine. The research was carried out from November 2021-February 2022 at the Screen House, Faculty of Agriculture, UNS. The plant materials used included P. violacea from Sulawesi, P. violacea from Java, and P. Ambilis from Ambon. The treatment used was colchicine 1,250 ppm which was applied to flower buds using the cotton method with 2 replications. The collected data were analyzed by descriptive analysis and paired T-test using IBM SPSS Statistics 26 to compare the average performance of mutant plants with control plants. Induction of polyploidy using colchicine with a concentration of 1,250 ppm could increase the size of leaves, flower stalks, flowers, dorsal and lateral sepals, and petals in P. violacea from Java, P. violacea from Sulawesi, and P. amabilis from Ambon.
Growth Response and Yields of Variety of Sorghum on Multiple NPK Fertilization in Dry Land Samanhudi Samanhudi; Puji Harsono; Sri Hartati; Iswahyudi Iswahyudi; Naufal Ghazy Wicaksono
Journal of Biodiversity and Biotechnology Vol 4, No 1 (2024)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v4i1.108195

Abstract

Land degradation is a major constraint to agricultural productivity, with drylands particularly vulnerable to critical conditions. Sorghum (Sorghum bicolor L. Moench) is a promising crop for such environments due to its adaptability and dual-purpose value for both biomass and sugar-rich stalks. This study evaluated the growth and yield response of four sorghum varieties—Super, Suri, Numbu, and Keller—to different rates of compound NPK fertilizer under dryland conditions. The experiment was arranged in a Randomized Complete Block Design (RCBD) with two factors: sorghum varieties and five fertilizer doses (0, 10, 12, 14, and 16 g plant⁻¹), replicated three times. Data were subjected to ANOVA, and treatment means were compared using DMRT at the 5% significance level. Results showed that the Keller variety consistently produced the best agronomic performance, including plant height, number of leaves, stem segments, stem diameter, and biomass dry weight. Moreover, Keller combined with 12 g plant⁻¹ of NPK fertilizer produced the highest sap content (14.0% Brix), highlighting its potential as a dual-purpose sorghum. Leaf traits were most responsive at 14 g plant⁻¹, indicating that moderate-to-high fertilization enhances vegetative growth without excessive input. These findings demonstrate that varietal selection coupled with appropriate nutrient management is crucial for optimizing sorghum production in dryland systems. The study provides practical guidance for farmers and can inform future breeding and fertilizer recommendation programs for sustainable sorghum cultivation.
Karyotype Analysis of Phalaenopsis Hibryds with Colchicine Induction Sri Hartati; Samanhudi Samanhudi; Hasna Afanin
Journal of Biodiversity and Biotechnology Vol 4, No 1 (2024)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v4i1.101961

Abstract

Phalaenopsis hybrid is one of the orchids that is very important in the horticultural market as an ornamental plant whose demand continues to increase. This causes the need for breeding in order to obtain new varied flower characters as desired by polyploidization. Polyploidy induction in Phalaenopsis hybrids was carried out using colchicine. The purpose of this study was to determine the karyotype pattern of Phalaenopsis hybrids. The research was conducted at the Screenhouse of the Faculty of Agriculture and Integrated Laboratory Unit of Sebelas Maret University, Surakarta in August 2021-March 2022. Polyploidy induction was carried out by dripping 1500 ppm concentration of colchicine on flower bud of Phalaenopsis hybrid. The method used in this research is squashing. The experimental results showed that the karyotype pattern in control Phal. Golden Tree was 2n=3x-7=50m, while in Phal. Fuller Sunset and Phal. OX X-ray was 2n=4x=76m. The karyotype pattern in orchids with colchicine induction was Phal. Golden Tree 2n=6x-14=100m, while in Phal. Fuller Sunset and Phal. OX X-ray 2n=8x=152m. 
In Vitro Effects of Yeast Extract and Indole-3-Acetic Acid on Shoot Emergence and Height in Mas Kirana Banana Samanhudi Samanhudi; Retna Bandriyati Arniputri; Sri Hartati; Iswahyudi Iswahyudi; Dewi Selvia Maulida Saputri
Journal of Biodiversity and Biotechnology Vol 5, No 1 (2025)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v5i1.108197

Abstract

Mas Kirana banana (Musa sp.) is a superior local variety from Lumajang, East Java, valued for its favorable fruit size and consumer preference. Conventional propagation of this variety is limited due to its low production of saplings (2–3 per clump), making large-scale cultivation inefficient. Tissue culture techniques offer an alternative approach to accelerate propagation. This study aimed to evaluate the effects of yeast extract and indole-3-acetic acid (IAA) on the in vitro growth of Mas Kirana banana explants. The experiment was arranged in a factorial completely randomized design (CRD) with two factors: yeast extract at four concentrations (0, 400, 800, and 1200 mg L⁻¹) and IAA at four concentrations (0, 0.75, 1.5, and 2.25 ppm). Growth responses were assessed based on shoot emergence, shoot height, root development, and leaf formation. Yeast extract significantly affected shoot emergence time; however, its effect was inhibitory rather than promotive. The control (0 mg L⁻¹) produced the fastest emergence (11 DAP), whereas higher yeast concentrations (400–1200 mg L⁻¹) delayed emergence to 13–22 DAP.. IAA significantly influenced shoot height, with the best performance at 1.5 ppm, while other growth parameters were not significantly affected. These findings indicate that the effectiveness of yeast extract and IAA is highly concentration-dependent, and their roles in improving micropropagation of Mas Kirana banana require further optimization. This study provides baseline information to support the development of more efficient tissue culture protocols for large-scale propagation of this important local variety.
The Effect of Naphtaleine Acetic Acid ND Benzyl Amino Purine on the Subculture of Kepok Unti Sayang Banana Samanhudi Samanhudi; Andriyana Setyawati; Edi Purwanto; Muji Rahayu; Eviani Eviani; Muhammad Aji Cahyadi
Journal of Biodiversity and Biotechnology Vol 5, No 1 (2025)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v5i1.108513

Abstract

The Kepok Unti Sayang variety is a superior type of banana that is resistant to fusarium wilt. The high demand for Kepok Unti Sayang needs to be balanced with good seed production. One of the methods used for multiplying superior seeds of Kepok Unti Sayang is through tissue culture. This research aims to obtain the optimal concentration of Naphtaleine Acetic Acid (NAA) in the subculture media for the growth of root numbers in the Kepok Unti Sayang Banana. The second objective is to determine the optimal concentration of Benzyl Amino Purine (BAP) in the subculture medium for shoot number growth in Kepok Unti Sayang bananas. Another goal is to identify the best treatment combination that can produce the highest number of shoots in Kepok Unti Sayang bananas. The research was conducted at the Laboratory of Plant Physiology and Biotechnology at Sebelas Maret University from February to July 2025. The study was conducted with 4 levels of NAA treatment: 0; 0.5; 1; and 1.5 ppm. The BAP treatment was administered at 4 levels: 0; 1; 2; and 3 ppm. The variables observed in this study were the time of shoot emergence, number of shoots, time of root emergence, root length, and final height of the planlet. Data from each variable were then analyzed using analysis of variance (ANOVA) with an F-test at a significance level of 95%. Significantly different data were then followed by Duncan's Multiple Range Test (DMRT) at a significance level of 95%. The significant combination of NAA and BAP on the observed variables was then reanalyzed using regression tests. The results showed that the application of various concentrations of NAA does not affect the growth of the number of roots of Kepok bananas var. Unti Sayang. The application of 2 ppm BAP is optimal for the growth of the number of shoots of Kepok bananas var. Unti Sayang. The interaction between 1 ppm NAA and BAP at 1-2 ppm produces the most optimal average shoot emergence time.
Application Cow Manure and Mychorriza to Physiological of Biduri (Calotropis gigantea) Sri Hartati; Samanhudi Samanhudi; Muji Rahayu; Mochammad Danny Sukardan; Nadia Alifia Rahma
Journal of Biodiversity and Biotechnology Vol 2, No 2 (2022)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v2i2.61273

Abstract

Biduri (Calotropis gigantea) was a wild plant that had many benefits in the health and textile industries. In the health sector, biduri was used as a herbal plant, while in the textile industry, used as the main raw material. Biduri could be used as a substitute for kapok as the main raw material, therefore it was necessary to develop cultivation technology to met the needs. This research was conducted at the Jumantono Field Laboratory of Faculty of Agriculture UNS, Karanganyar using a factorial Randomized Complete Block Design (RCBD), which consisted of 2 factors, namely cow dung fertilizer, and mycorrhizae. Cow dung fertilizer consists of 0 ton/ha, 10 ton/ha, and 20 ton/ha. Mycorrhizae consisted of 0 grams/plant, 5 grams/plant, 10gram/plant, and 15gram/plant. There were 36 treatment combination that consist of 12 treatment combination repeated 3 times. The interaction between cow dung did not significantly affect all physiological observation variables. The results showed that the application of 20 ton/ha of cow dung fertilizer gave the best results on the width of stomata openings, the rate of photosynthesis, and the rate of transpiration. Inoculation with mycorrhizal fungi had no significant effect on all observations of physiological variables Keywords: Calotropis gigantea, cow manure, mycorrhiza, physiological
APPLICATION OF BENZYL ADENINE AND IAA ON THE GROWTH OF BANANA BARANGAN SUBCULTURE (Musa acuminata L.) Andriyana Setyawati; Samanhudi Samanhudi; Edi Purwanto; Retna Bandriyati Arniputri; Kevin Putra Setyawan; Muhammad Aji Cahyadi
Journal of Biodiversity and Biotechnology Vol 4, No 2 (2024)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v4i2.108497

Abstract

Barangan banana is a banana variety that has high economic value, but its production is still carried out conventionally, resulting in limited barangan bananas on the market. The use of tissue culture methods can quickly produce high-quality, disease-free plants regardless of seasonal conditions. The aim of this research is to determine the effect of providing the best ZPT IAA and BA for the growth of the Barangan banana planet. The research was conducted in August-October 2023 at the Biotechnology and Plant Physiology Laboratory, Sebelas Maret University. This research used a two-factor Completely Randomized Design (CRD) with 6 replications. The first factor was the use of ZPT Indole Acetic Acid (concentrations 0, 0.5, 1, and 1.5 ppm and the second factor was the use of ZPT Benzyl Adenine (0, 0.5, 1, 1.5 ppm). The variables observed were root emergence time, number of roots, root length, time of leaf emergence, number of leaves, time of shoot emergence, number of shoots, and height of shoots. The observed data were analyzed using Analysis of Variance (ANOVA) with a 5% level test and if there was a significant difference, it was continued with Duncan's Multiple Range Test (DMRT) at the level 5%. The results showed that giving 0.5 ppm IAA increased root length; 0.5 ppm IAA was given optimally for root formation; and 1 ppm IAA increased the shoot height of the Barangan banana plnalet. Giving BA 0.5 ppm root number; BA 1, 5 ppm produces the highest number of leaves; BA 1 ppm increases shoot height of barangan banana plantlets.
Co-Authors Adi Ratriyanto Agastya Putra Pradipta Agnofi Merdeka Efendi AHMAD YUNUS Ahmad Yunus Ahmad Yunus Alfida Muthi’ah AMALIA TETRANI SAKYA AMALIA TETRANI SAKYA Amalia Tetrani Sakya Amalia Tetrani Sakya Amalia Tetrani Sakya Amalia Tetrani Sakya Amalia Tetrani Sakya Amalia Tetrani Sakya Amalia Tetrani Sakya Amalia Tetrani Sakya, Amalia Tetrani ANDRIYANA SETYAWATI ANDRIYANA SETYAWATI Andriyana Setiyawati Andriyana Setyawati Anif Nur Artanti, Anif Nur Anindya Saras ANIVERSARI APRIANA Anne Sukmawati ARI SUSILOWATI ATMITRI SISHARMINI Ayu Lestari BAMBANG PUJIASMANTO Bambang Pujiasmanto Bambang Pujiasmanto Bambang Pujiasmanto Bambang Pujiasmanto Bambang Pujiasmanto Dewi Selvia Maulida Saputri Dewi, Reni Surya Dhimas Taufika Putra DIDIK SUDIHARDJO Djoko Purnomo Djoko Purnomo Djoko Purnomo Dwi Hardjoko Dwi Harjoko Eddy Tri Haryanto EDI PURWANTO EDI PURWANTO EDI PURWANTO Edi Purwanto Edi Purwanto EDI PURWANTO EDI PURWANTO Edi Purwanto Edi Purwanto Edi Purwanto Edi Purwanto Edi Purwanto Edi Purwanto EKA HANDAYANTA Elvy Carolina Pane ENDANG YUNIASTUTI Endang Setyowati ENDANG YUNIASTUTI Erni Irawati Eviani Eviani FAROUQ HEIDAR BARIDO Fitri Aprillia Fitria Roviqowati Fitrotunnisa Al-batul Gani Cahyo Gani Cahyo Handoyo Gani Cahyo Handoyo Hadiandra Harfiansyah Hardjoko, Dwi HASAN BASRI JUMIN Hassan Bashir Hassan Bashir Himawan Joko Riswanda Ida Rumia Manurung Ida Rumia Manurung Ida Rumia Manurung Indah Tri Retnosari Indrawan Fahmi Iswahyudi Iswahyudi Iswahyudi Iswahyudi Iswahyudi Iswahyudi Iswahyudi Iwan Dewangga Joko Prihanto Kevin Putra Setyawan Lixin Zhang MARINA RUZYATI Mochammad Danny Sukardan Muhammad Aji Cahyadi Muhammad Imam Wicaksono Muhammad Imam Wicaksono Muhammad Naufal Adani MUJI RAHAYU Muji Rahayu Muji Rahayu Muji Rahayu MUMTAZ MUAWANAH Nadia Alifia Rahma Nandariyah, Nandariyah Naufal Ghazy Wicaksono Nugraheni Nugraheni NUR AZIZAH USWATUN HASANAH NUR AZIZAH USWATUN HASANAH Nurkholis Majid Okky Talitha ONGKO CAHYONO ONGKO CAHYONO Paramasari Dirgahayu PUJI HARSONO PUJI HARSONO Puji Harsono Purwanto Purwanto Qonita Rahma Dwiyanti Rahayu, Ria Muji Rahmanto Rahmanto Retna Bandriyati Arniputri Retna Bandriyati Arniputri Retna Bandriyati Arniputri Retna Bandriyati Arniputri Retna Bandriyati Arniputri Riandy Adhitya Rina Puji Lestari RIZKA NOVI SESANTI S.Pd. M Kes I Ketut Sudiana . Saat Egra Saras, Anindya Satya Primasasta Zulhivan SELVIA SUTRIANA SETIYANINGSIH, ARY SHOLAHUDDIN SHOLAHUDDIN Sigit PRASTOWO Siti Mardhika Sari Sri Hartati SRI HARTATI Sri Hartati Sri Hartati Sri Hartati Sri Hartati Sri Hartati Sri Hartati Sri Hartati Sri Mulyani SUDADI SUDADI Sudarmi Sudarmi Sulandjari Sulandjari, Sulandjari Supriyono Supriyono SUSANTI INDRIYA WATI TRI JOKO SANTOSO Tyas Alivia Wulandari Venty Suryanti Wartoyo Wartoyo Zuraida Anniswa Bachtiar