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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Caraka Tani - Agriculture Science Journal JURNAL ILMU PERTANIAN JURNAL PEMULIAAN TANAMAN HUTAN AGRIVITA, Journal of Agricultural Science Kultivasi Agrosains: Jurnal Penelitian Agronomi Caraka Tani: Journal of Sustainable Agriculture Asian Journal of Agriculture Agrotechnology Research Journal PRIMA: Journal of Community Empowering and Services Tropical Plantation Journal Journal of Biodiversity and Biotechnology Indonesian Journal of Jamu
Samanhudi Samanhudi
Department Of Agrotechnology, Faculty Of Agriculture, Universitas Sebelas Maret
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Economics, Econometrics & Finance Environmental Science Health Professions Medicine & Pharmacology

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In Vitro Propagation of Tribulus terrestris with IAA and BAP Concentrations Samanhudi Samanhudi; Ahmad Yunus; Rahmanto Rahmanto
Journal of Biodiversity and Biotechnology Vol 1, No 1 (2021)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v1i1.50245

Abstract

Tribulus terrestris is conventionally propagated using seeds but has limited germination capacity. One approach that can be done for this plant multiplication is in vitro tissue culture. This study was conducted to determine the growth potential of T. terrestris in vitro by the addition of auxin (IAA) and cytokinin (BAP) as growth regulators at several concentrations. The study was conducted at the Laboratory of Plant Physiology and Biotechnology, Faculty of Agriculture, Universitas Sebelas Maret Surakarta from February to December 2017. The experiment employed factorial Completely Randomized Design (CRD) method with 2 factors, namely IAA concentration (0 ppm, 0.1 ppm, 0.2 ppm, 0.3 ppm), and BAP concentration (0 ppm, 0.3 ppm, 0.5 ppm, 0.7 ppm). The observed growth parameters were shoot emergence time, leaf emergence time, root emergence time, number of leaves, and number of roots. Data were analyzed using analysis of variance followed by DMRT with a 95% confidence level. The results showed that the addition of IAA only affects leaf emergence time, while the addition of BAP had a significant effect on the leaf emergence time and number of leaves. The interactions between IAA and BAP had a significant effect on leaf emergence time. Keywords: explant; plant growth; tissue culture.
Comparison between the electrical conductivity method and radicle emergence test as a rapid test of sorghum seed vigor Nugraheni, Nugraheni; Pujiasmanto, Bambang; Samanhudi, Samanhudi; Sakya, Amalia Tetrani
Kultivasi Vol 22, No 2 (2023): Jurnal Kultivasi
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/kultivasi.v22i2.46547

Abstract

Sorghum (Sorghum bicolor L. Moench) belongs to multipurpose crops. All parts of sorghum can be used both as main products and derivative products; some derivative products produced from the sorghum plant consist of sugar, bioethanol, biomass, handicraft raw materials and starch. This study aims to determine the time required for testing the vigor and viability of sorghum seeds using the electrical conductivity and radicle emergence methods. The study was conducted in two experimental stages using a completely randomized design. The first experiment consisted of two factors: the number of sorghum seeds (40, 70 and 100 grains) and the volume of soaking water (75, 100, 125, and 150 ml). The second experiment consisted of two factors: research method (germination/control method, electrical conduction method, and radicle emergence method) and varieties (consisted of Numbu, Kawali, Suri 3, and Suri 4). The first experiment's results showed that the best electrical conductivity method on Suri 4 varieties were 40 seeds and 150 ml water volume. The electrical conductivity value is negatively correlated with Germination capacity, vigor index, growth of speed, maximum growth potential, and sprout growth rate. Time needed for Electrical Conductivity method in this study was three days. The germination period of 96 hours gives the best results on the radicle emergence of sorghum seed varieties. Vigor index, growth of speed, germination capacity, and maximum growth potential are positively correlated with the value of radicula emergence.Keywords: Electrical Conductivity, Radicle emergence, Vigor, Viability, Germination
Studies of Shading Levels and Nutrition Sources on Growth, Yield and Andrographolide Content of Sambiloto (Andrographis paniculata Ness) Purwanto, Edi; Samanhudi, Samanhudi; Sudarmi, Sudarmi
AGRIVITA Journal of Agricultural Science Vol 33, No 3 (2011)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v33i3.73

Abstract

Growth and biochemical content of medicinal crops are influenced by agroecosystems characteristics . The objective of this research was to determine the optimum shading level and type of fertilizer as sources of nutrition on the growth, yield, and andrographolide content of sambiloto. The experiment used Split Plot Design with basic design of Randomized Complete Block Design arranged with two treatment factors, with three replications. The first factor as the main plot was shading levels, namely without shading, 25% shading, 50% shading, and 75% shading. The second factor as the sub plot was sources of nutrition reprented by type of fertilizer, namely NPK fertilizer, cow stable fertilizer, and compost fertilizer. The result of research indicated that shading level and the kind of nutrition influenced some growth and yield variables such as number of leaves, number of branches, plant height, plant dry weight and simplisia weight, and andrographolide content. Interaction of shading level at 25% and straw compost fertilizer performed best in growth characteristics, while the highest andrographolide content resulted from the treatment combination of 50% shading level and straw compost fertilizer.Keywords: fertilizer,nutrition, shading, andrographolide, Andrographis paniculata
In Vitro Propagation of Temulawak (Curcuma xanthorrhiza Roxb.) Samanhudi, Samanhudi; Yunus, Ahmad; Pujiasmanto, Bambang; Saras, Anindya
Jurnal Jamu Indonesia Vol. 2 No. 2 (2017): Jurnal Jamu Indonesia
Publisher : Tropical Biopharmaca Research Center, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jji.v2i2.30

Abstract

Problems encountered in the development of herbal medicine industry is that most of the raw materials (80%) came from the forest or natural habitats and the rest (20%) of the results of traditional cultivation. The research is focused to get the propagation techniques of temulawak by in vitro using various combinations of IBA and BAP concentration, each consisting of 0, 1, 2, 3, 4 ppm. The purpose of this research is to gain a plant propagation technique of temulawak through the use of IBA and BAP concentration so as to provide a quality seeds. The results showed that the highest shoots obtained at 1 ppm IBA and 3 ppm BAP treatment with a buds height of 15.9 cm. Treatment of 0 ppm IBA and 4 ppm BAP produces the highest buds number by 4 buds. While the highest number of leaves obtained in 2 ppm IBA and 3 ppm BAP treatment with total of 18 leafs. The highest length of leaf obtained in the treatment of 3 ppm IBA and 2 ppm BAP with a length of 14.6 cm. Variable highest number of roots was obtained at treatment of 4 ppm IBA and 1 ppm BAP with root number exceeds 30 pieces with very many hair roots and the highest root length was obtained at treatment of 2 ppm IBA and 0 ppm BAP with a length of 15.6 cm. Based on these results it can be concluded that the IBA and BAP treatment at various concentrations can affect the propagation and growth of temulawak explants.
Morphological characteristics of Phalaenopsis hybrids treated with 1500 ppm colchicine Samanhudi, Samanhudi; Hartati, Sri; Manurung, Ida Rumia; Lestari, Ayu
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 53 No. 3 (2025): Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy)
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24831/jai.v53i3.64689

Abstract

The increasing number of Phalaenopsis sp. enthusiasts needs to be balanced with the availability of good-quality cultivars, one of which can be achieved by improving flower morphological characteristics through colchicine induction. Colchicine-induced plants often exhibit superior traits such as larger, thicker, and more durable flowers. This study aimed to investigate the effect of a 1500 ppm colchicine concentration on the floral morphological characteristics of the Phalaenopsis sp., with a particular focus on flower size, petal thickness, and sepal morphology. This research provides genetic diversity information on Phalaenopsis sp., which can serve as a basis for future breeding programs and as a reference material for orchid growers and researchers. The materials used were flower buds of three Phalaenopsis varieties: Phalaenopsis Fuller’s Sunset, Phalaenopsis Golden Tree, and Phalaenopsis OX X-Ray. Each treatment was replicated three times. Morphological data were analyzed qualitatively through scoring and converted into binary data according to the Guide Book of Orchid Plant Characterization by the Ornamental Plant Research Center (2007). The results showed that 1500 ppm colchicine affects Phalaenopsis Fullers Sunset in the form of rhombus-shaped petals, and on Phalaenopsis Golden Tree in the form of pointed lateral sepal tips, concave transverse and longitudinal cross-sections of petals, and on Phalaenopsis OX X-Ray in the form of split dorsal sepal edges, and open petal arrangement. Overall, colchicine treatment also induced apparent enlargement of flowers in all three Phalaenopsis hybrids. Keywords: chromosome; flower morphology; mutagenesis; ornamental plant; polyploidy
Rice response to cadmium bioremediation using sorghum and mycorrhiza HARSONO, PUJI; HASANAH, NUR AZIZAH USWATUN; PURWANTO, EDI; SAMANHUDI, SAMANHUDI
Asian Journal of Agriculture Vol. 9 No. 2 (2025)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/asianjagric/g090219

Abstract

Abstract. Harsono P, Hasanah NAU, Purwanto E, Samanhudi. 2025. Rice response to cadmium bioremediation using sorghum and mycorrhiza. Asian J Agric 9: 522-532. Cadmium (Cd) contamination at 2.9 mg kg-¹ in paddy soils reduces rice productivity and threatens food safety. This study aimed to evaluate the effectiveness of sorghum (Sorghum bicolor) phytoremediation combined with Arbuscular Mycorrhizal Fungi (AMF) in improving rice growth under mild Cd contamination. A factorial randomized complete block design with two cropping systems (seed vs. ratoon, where the same crop is re-grown from the stubble or roots of the first crop) and seven remediation treatments (control; three sorghum varieties (Super 1, Samurai 1, Kawali) combine with/without 10 g AMF plot-¹) was applied in 42 plots (3×4 m). Rice cv. IR 64 was transplanted 30 days after remediation. Significant interactions (p<0.05) between cropping system and remediation treatment occurred for plant height and leaf area. The Kawali + AMF treatment produced the most significant gains relative to the control: plant height +7.7%, leaf area +207%, chlorophyll +236% and total dry biomass +152%, while extending the vegetative phase by 23%. Improvements were consistent across sorghum seed and sorghum ratoon systems, indicating that remediation benefits persist beyond the first harvest. Ratoon sorghum-maintained remediation benefits, with 7-10% taller plants and 8% greater leaf area than the control, despite slightly reduced canopy size compared with seed sorghum; all sorghum treatments delayed flowering by ~11 days, indicating reduced Cd stress. High-biomass sorghum, especially Kawali, combined with AMF, enhances rice growth and physiology while stabilizing Cd, providing a scalable remediation strategy for sustainable rice production in Cd-contaminated tropical agroecosystems.
Effects of nutrient solution and substrate on Limnocharis flava performance in hydroponic systems SESANTI, RIZKA NOVI; PURWANTO, EDI; SAMANHUDI, SAMANHUDI; SUDADI, SUDADI
Asian Journal of Agriculture Vol. 9 No. 2 (2025)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/asianjagric/g090225

Abstract

Abstract. Sesanti RN, Purwanto E, Samanhudi, Sudadi. 2025. Effects of nutrient solution and substrate on Limnocharis flava performance in hydroponic systems. Asian J Agric 9: 590-597. Limnocharis flava is a promising aquatic vegetable for substrate-based hydroponic cultivation. However, the optimal combination of nutrient concentrations and substrate types to maximize growth under hydroponic conditions remains unclear. This study evaluated five nutrient solution concentrations (0, 0.8, 1.6, 2.4, and 3.2 dS m-¹) and three substrate types (volcanic sand, rice husk charcoal, and a 1:1 mixture) in a Randomized Complete Block Design (RCBD) with three replications. Nutrient concentrations of 0.8 and 1.6 dS m-¹ produced the highest plant height (28.48 cm and 28.20 cm), number of leaves (11.31 and 11.03), shoot dry weight (4.61 g and 4.74 g), root fresh weight (13.20 g and 12.43 g), and root dry weight (0.76 g and 0.74 g). Both lower and higher concentrations inhibited growth, indicating optimal thresholds for nutrient uptake. Chlorophyll a, b, and total chlorophyll contents were similar across the 0.8-3.2 dS m-¹ range, but consistently higher than the control. The leaf greenness index showed a significant interaction between nutrient concentration and substrate type, with the highest value at 2.4 dS m-¹ in the mixed substrate. A nutrient concentration of 1.6 dS m-¹ produced the best overall performance. Based on these results, a combination of 1.6 dS m-¹ nutrient solution and a 1:1 mixture of volcanic sand and rice husk charcoal is recommended for the cultivation of L. flava. These findings establish baseline guidelines for hydroponic cultivation of L. flava and highlight its potential to advance precision hydroponics for indigenous vegetables, thereby contributing to food security and sustainable agriculture.
Effect of Dolomite and NPK on The Growth and Yield of off-Season Shallots Iswahyudi, Iswahyudi; Haryanto, Eddy Tri; Supriyono, Supriyono; Al-batul, Fitrotunnisa; Samanhudi, Samanhudi; Irawati, Erni; Rahayu, Muji; Purwanto, Edi
Tropical Plantation Journal Vol 5, No 1 (2026): TROPICAL PLANTATION JOURNAL
Publisher : Akademi Komunitas Perkebunan Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56125/tpj.v5i1.74

Abstract

Shallot is a high-value horticultural commodity. Its productivity often declines due to suboptimal soil conditions, such as low pH and imbalanced nutrient availability, especially when cultivated off-season. This study aimed to determine the optimal combination of dolomite and NPK (Nitrogen, Phosphorus, Potassium) fertilizer for the growth and yield of shallot cultivated in the off-season. The research was conducted in Ngringo Village, Jaten District, Karanganyar Regency from February-April 2025. The method used was a factorial Completely Randomized Design (CRD) with two factors and three replications. The first factor was dolomite doses (0; 2000; and 4000 kg.ha-1) and NPK fertilizer doses (250; 375; and 500 kg.ha-1). Observed parameters were plant height, number of leaves, fresh and dry biomass weight, fresh and dry bulb weight, number of bulbs per plant, and bulb diameter. Data were analyzed by analysis of variance (ANOVA) at a 95% significance level and continued with Duncan’s Multiple Range Test (DMRT) with 95% significance level when significant differences were found. The results showed no significant interaction between dolomite and NPK fertilizer on shallot growth and yield. Dolomite at 2000 kg.ha-1 significantly increased the number of leaves, while NPK fertilizer had no significant effect. However, 250 kg.ha-1 of NPK fertilizer produced similar results to higher doses, indicating potential fertilizer efficiency. Environmental conditions such as high humidity and low light intensity likely influenced treatment effectiveness.
Co-Authors Agastya Putra Pradipta Agnofi Merdeka Efendi Ahmad Yunus Ahmad Yunus Ahmad Yunus Ahmad Yunus Al-batul, Fitrotunnisa Amalia Tetrani Sakya Amalia Tetrani Sakya Amalia Tetrani Sakya, Amalia Tetrani AYU LESTARI Bambang Pujiasmanto Bambang Pujiasmanto Bambang Pujiasmanto Bambang Pujiasmanto Dewi, Reni Surya Dhimas Taufika Putra Dwi Hardjoko Eddy Tri Haryanto, Eddy Tri Edi Purwanto Edi Purwanto Edi Purwanto Edi Purwanto Erni Irawati Fitri Aprillia Hadiandra Harfiansyah Hardjoko, Dwi HASANAH, NUR AZIZAH USWATUN Himawan Joko Riswanda Ida Rumia Manurung Indah Tri Retnosari Indrawan Fahmi ISWAHYUDI ISWAHYUDI Iwan Dewangga Muhammad Imam Wicaksono Muhammad Imam Wicaksono Muji Rahayu Muji Rahayu Nugraheni Nugraheni Nurkholis Majid Puji Harsono, Puji Purwanto Purwanto Rahmanto Rahmanto Riandy Adhitya Rizka Novi Sesanti S.Pd. M Kes I Ketut Sudiana . Saras, Anindya SETIYANINGSIH, ARY Siti Mardhika Sari Sri Hartati Sudadi Sudadi Sudarmi Sudarmi Sulandjari Sulandjari, Sulandjari Supriyono Supriyono Wartoyo Wartoyo Yunus, Ahmad