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All Journal Jurnal Hortikultura Indonesia (JHI) Caraka Tani - Agriculture Science Journal JURNAL ILMU PERTANIAN JURNAL PEMULIAAN TANAMAN HUTAN Kultivasi Agrosains: Jurnal Penelitian Agronomi Caraka Tani: Journal of Sustainable Agriculture Asian Journal of Agriculture Agrotechnology Research Journal PRIMA: Journal of Community Empowering and Services Journal of Biodiversity and Biotechnology
Samanhudi Samanhudi
Department Of Agrotechnology, Faculty Of Agriculture, Universitas Sebelas Maret
Agriculture, Biological Sciences & Forestry Environmental Science

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In Vitro Propagation of Tribulus terrestris with IAA and BAP Concentrations Samanhudi Samanhudi; Ahmad Yunus; Rahmanto Rahmanto
Journal of Biodiversity and Biotechnology Vol 1, No 1 (2021)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v1i1.50245

Abstract

Tribulus terrestris is conventionally propagated using seeds but has limited germination capacity. One approach that can be done for this plant multiplication is in vitro tissue culture. This study was conducted to determine the growth potential of T. terrestris in vitro by the addition of auxin (IAA) and cytokinin (BAP) as growth regulators at several concentrations. The study was conducted at the Laboratory of Plant Physiology and Biotechnology, Faculty of Agriculture, Universitas Sebelas Maret Surakarta from February to December 2017. The experiment employed factorial Completely Randomized Design (CRD) method with 2 factors, namely IAA concentration (0 ppm, 0.1 ppm, 0.2 ppm, 0.3 ppm), and BAP concentration (0 ppm, 0.3 ppm, 0.5 ppm, 0.7 ppm). The observed growth parameters were shoot emergence time, leaf emergence time, root emergence time, number of leaves, and number of roots. Data were analyzed using analysis of variance followed by DMRT with a 95% confidence level. The results showed that the addition of IAA only affects leaf emergence time, while the addition of BAP had a significant effect on the leaf emergence time and number of leaves. The interactions between IAA and BAP had a significant effect on leaf emergence time. Keywords: explant; plant growth; tissue culture.
Comparison between the electrical conductivity method and radicle emergence test as a rapid test of sorghum seed vigor Nugraheni, Nugraheni; Pujiasmanto, Bambang; Samanhudi, Samanhudi; Sakya, Amalia Tetrani
Kultivasi Vol 22, No 2 (2023): Jurnal Kultivasi
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/kultivasi.v22i2.46547

Abstract

Sorghum (Sorghum bicolor L. Moench) belongs to multipurpose crops. All parts of sorghum can be used both as main products and derivative products; some derivative products produced from the sorghum plant consist of sugar, bioethanol, biomass, handicraft raw materials and starch. This study aims to determine the time required for testing the vigor and viability of sorghum seeds using the electrical conductivity and radicle emergence methods. The study was conducted in two experimental stages using a completely randomized design. The first experiment consisted of two factors: the number of sorghum seeds (40, 70 and 100 grains) and the volume of soaking water (75, 100, 125, and 150 ml). The second experiment consisted of two factors: research method (germination/control method, electrical conduction method, and radicle emergence method) and varieties (consisted of Numbu, Kawali, Suri 3, and Suri 4). The first experiment's results showed that the best electrical conductivity method on Suri 4 varieties were 40 seeds and 150 ml water volume. The electrical conductivity value is negatively correlated with Germination capacity, vigor index, growth of speed, maximum growth potential, and sprout growth rate. Time needed for Electrical Conductivity method in this study was three days. The germination period of 96 hours gives the best results on the radicle emergence of sorghum seed varieties. Vigor index, growth of speed, germination capacity, and maximum growth potential are positively correlated with the value of radicula emergence.Keywords: Electrical Conductivity, Radicle emergence, Vigor, Viability, Germination
In Vitro Proliferation and Acclimatization of Kepok Banana Unti Sayang (ABB) with Addition of Organic Materials Devinawati Rachmi; Samanhudi; Djoko Purnomo
Jurnal Hortikultura Indonesia (JHI) Vol 11 No 2 (2020): Jurnal Hortikultura Indonesia
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jhi.11.2.91-100

Abstract

Kepok banana (Musa balbisiana) contains genome B with high browning potential and low proliferation rate in vitro culture. One way to reduce browning and increase levels of vitamins and hormones, is by adding synthetic supplements and natural extracts. This study aimed to determine the proliferation of kepok banana Unti Sayang explants in fruit extract enriched media and its plantlet acclimatization ability. This research consisted of 2 experiments, the first stage was in vitro using a split plot completed randomized design with two factors: main plot (3 levels of temperature of culture room) and subplot (5 levels of fruit extracts). The second stage was the plantlet acclimatization using a split-plot completed randomized design with two factors: main plot (4 levels of acclimatization media) and subplot (4 levels of plantlet vigor). The results showed that the addition of fruit extracts to MS media affected meanwhile the incubation temperature difference did not affect the proliferation of kepok banana Unti Sayang explants. Papaya fruit extract (150 g L-1) and coconut water (100 ml L-1) gave the same results with the addition of synthetic vitamins to the proliferation and morphogenesis of kepok banana Unti Sayang. Plantlet vigor in various acclimatization media is not significantly different, with the growth ability up to 92%.
Co-Authors Agastya Putra Pradipta Agnofi Merdeka Efendi Ahmad Yunus Ahmad Yunus Ahmad Yunus Ahmad Yunus Amalia Tetrani Sakya Amalia Tetrani Sakya Amalia Tetrani Sakya, Amalia Tetrani Bambang Pujiasmanto Bambang Pujiasmanto Bambang Pujiasmanto Bambang Pujiasmanto Devinawati Rachmi Dewi, Reni Surya Dhimas Taufika Putra Djoko Purnomo Dwi Hardjoko Edi Purwanto Edi Purwanto Edi Purwanto Edi Purwanto Fitri Aprillia Hadiandra Harfiansyah Hardjoko, Dwi Himawan Joko Riswanda Indah Tri Retnosari Indrawan Fahmi Iwan Dewangga Muhammad Imam Wicaksono Muhammad Imam Wicaksono Muji Rahayu Muji Rahayu Nugraheni Nugraheni Nurkholis Majid Purwanto Purwanto Rahmanto Rahmanto Riandy Adhitya S.Pd. M Kes I Ketut Sudiana . SETIYANINGSIH, ARY Siti Mardhika Sari Sulandjari Sulandjari, Sulandjari Wartoyo Wartoyo Yunus, Ahmad