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All Journal Caraka Tani - Agriculture Science Journal JURNAL ILMU PERTANIAN JURNAL PEMULIAAN TANAMAN HUTAN AGRIVITA, Journal of Agricultural Science Kultivasi Agrosains: Jurnal Penelitian Agronomi Caraka Tani: Journal of Sustainable Agriculture Asian Journal of Agriculture Agrotechnology Research Journal PRIMA: Journal of Community Empowering and Services Journal of Biodiversity and Biotechnology Indonesian Journal of Jamu
Samanhudi Samanhudi
Department Of Agrotechnology, Faculty Of Agriculture, Universitas Sebelas Maret
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Environmental Science Health Professions Medicine & Pharmacology

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In Vitro Propagation of Tribulus terrestris with IAA and BAP Concentrations Samanhudi Samanhudi; Ahmad Yunus; Rahmanto Rahmanto
Journal of Biodiversity and Biotechnology Vol 1, No 1 (2021)
Publisher : Pusat Penelitian dan Pengembangan Bioteknologi dan Biodiversitas (P3BB) LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jbb.v1i1.50245

Abstract

Tribulus terrestris is conventionally propagated using seeds but has limited germination capacity. One approach that can be done for this plant multiplication is in vitro tissue culture. This study was conducted to determine the growth potential of T. terrestris in vitro by the addition of auxin (IAA) and cytokinin (BAP) as growth regulators at several concentrations. The study was conducted at the Laboratory of Plant Physiology and Biotechnology, Faculty of Agriculture, Universitas Sebelas Maret Surakarta from February to December 2017. The experiment employed factorial Completely Randomized Design (CRD) method with 2 factors, namely IAA concentration (0 ppm, 0.1 ppm, 0.2 ppm, 0.3 ppm), and BAP concentration (0 ppm, 0.3 ppm, 0.5 ppm, 0.7 ppm). The observed growth parameters were shoot emergence time, leaf emergence time, root emergence time, number of leaves, and number of roots. Data were analyzed using analysis of variance followed by DMRT with a 95% confidence level. The results showed that the addition of IAA only affects leaf emergence time, while the addition of BAP had a significant effect on the leaf emergence time and number of leaves. The interactions between IAA and BAP had a significant effect on leaf emergence time. Keywords: explant; plant growth; tissue culture.
Comparison between the electrical conductivity method and radicle emergence test as a rapid test of sorghum seed vigor Nugraheni, Nugraheni; Pujiasmanto, Bambang; Samanhudi, Samanhudi; Sakya, Amalia Tetrani
Kultivasi Vol 22, No 2 (2023): Jurnal Kultivasi
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/kultivasi.v22i2.46547

Abstract

Sorghum (Sorghum bicolor L. Moench) belongs to multipurpose crops. All parts of sorghum can be used both as main products and derivative products; some derivative products produced from the sorghum plant consist of sugar, bioethanol, biomass, handicraft raw materials and starch. This study aims to determine the time required for testing the vigor and viability of sorghum seeds using the electrical conductivity and radicle emergence methods. The study was conducted in two experimental stages using a completely randomized design. The first experiment consisted of two factors: the number of sorghum seeds (40, 70 and 100 grains) and the volume of soaking water (75, 100, 125, and 150 ml). The second experiment consisted of two factors: research method (germination/control method, electrical conduction method, and radicle emergence method) and varieties (consisted of Numbu, Kawali, Suri 3, and Suri 4). The first experiment's results showed that the best electrical conductivity method on Suri 4 varieties were 40 seeds and 150 ml water volume. The electrical conductivity value is negatively correlated with Germination capacity, vigor index, growth of speed, maximum growth potential, and sprout growth rate. Time needed for Electrical Conductivity method in this study was three days. The germination period of 96 hours gives the best results on the radicle emergence of sorghum seed varieties. Vigor index, growth of speed, germination capacity, and maximum growth potential are positively correlated with the value of radicula emergence.Keywords: Electrical Conductivity, Radicle emergence, Vigor, Viability, Germination
Studies of Shading Levels and Nutrition Sources on Growth, Yield and Andrographolide Content of Sambiloto (Andrographis paniculata Ness) Purwanto, Edi; Samanhudi, Samanhudi; Sudarmi, Sudarmi
AGRIVITA Journal of Agricultural Science Vol 33, No 3 (2011)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v33i3.73

Abstract

Growth and biochemical content of medicinal crops are influenced by agroecosystems characteristics . The objective of this research was to determine the optimum shading level and type of fertilizer as sources of nutrition on the growth, yield, and andrographolide content of sambiloto. The experiment used Split Plot Design with basic design of Randomized Complete Block Design arranged with two treatment factors, with three replications. The first factor as the main plot was shading levels, namely without shading, 25% shading, 50% shading, and 75% shading. The second factor as the sub plot was sources of nutrition reprented by type of fertilizer, namely NPK fertilizer, cow stable fertilizer, and compost fertilizer. The result of research indicated that shading level and the kind of nutrition influenced some growth and yield variables such as number of leaves, number of branches, plant height, plant dry weight and simplisia weight, and andrographolide content. Interaction of shading level at 25% and straw compost fertilizer performed best in growth characteristics, while the highest andrographolide content resulted from the treatment combination of 50% shading level and straw compost fertilizer.Keywords: fertilizer,nutrition, shading, andrographolide, Andrographis paniculata
In Vitro Propagation of Temulawak (Curcuma xanthorrhiza Roxb.) Samanhudi, Samanhudi; Yunus, Ahmad; Pujiasmanto, Bambang; Saras, Anindya
Jurnal Jamu Indonesia Vol. 2 No. 2 (2017): Jurnal Jamu Indonesia
Publisher : Tropical Biopharmaca Research Center, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jji.v2i2.30

Abstract

Problems encountered in the development of herbal medicine industry is that most of the raw materials (80%) came from the forest or natural habitats and the rest (20%) of the results of traditional cultivation. The research is focused to get the propagation techniques of temulawak by in vitro using various combinations of IBA and BAP concentration, each consisting of 0, 1, 2, 3, 4 ppm. The purpose of this research is to gain a plant propagation technique of temulawak through the use of IBA and BAP concentration so as to provide a quality seeds. The results showed that the highest shoots obtained at 1 ppm IBA and 3 ppm BAP treatment with a buds height of 15.9 cm. Treatment of 0 ppm IBA and 4 ppm BAP produces the highest buds number by 4 buds. While the highest number of leaves obtained in 2 ppm IBA and 3 ppm BAP treatment with total of 18 leafs. The highest length of leaf obtained in the treatment of 3 ppm IBA and 2 ppm BAP with a length of 14.6 cm. Variable highest number of roots was obtained at treatment of 4 ppm IBA and 1 ppm BAP with root number exceeds 30 pieces with very many hair roots and the highest root length was obtained at treatment of 2 ppm IBA and 0 ppm BAP with a length of 15.6 cm. Based on these results it can be concluded that the IBA and BAP treatment at various concentrations can affect the propagation and growth of temulawak explants.
Co-Authors Agastya Putra Pradipta Agnofi Merdeka Efendi Ahmad Yunus Ahmad Yunus Ahmad Yunus Ahmad Yunus Amalia Tetrani Sakya Amalia Tetrani Sakya Amalia Tetrani Sakya, Amalia Tetrani Bambang Pujiasmanto Bambang Pujiasmanto Bambang Pujiasmanto Bambang Pujiasmanto Dewi, Reni Surya Dhimas Taufika Putra Dwi Hardjoko Edi Purwanto Edi Purwanto Edi Purwanto Edi Purwanto Fitri Aprillia Hadiandra Harfiansyah Hardjoko, Dwi Himawan Joko Riswanda Indah Tri Retnosari Indrawan Fahmi Iwan Dewangga Muhammad Imam Wicaksono Muhammad Imam Wicaksono Muji Rahayu Muji Rahayu Nugraheni Nugraheni Nurkholis Majid Purwanto Purwanto Rahmanto Rahmanto Riandy Adhitya S.Pd. M Kes I Ketut Sudiana . Saras, Anindya SETIYANINGSIH, ARY Siti Mardhika Sari Sudarmi Sudarmi Sulandjari Sulandjari, Sulandjari Wartoyo Wartoyo Yunus, Ahmad