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STATUS KEBERSIHAN RONGGA MULUT DAN KEBUTUHAN PERAWATAN JARINGAN PERIODONTAL PADA LANSIA DI DAERAH PESISIR PUGER, KABUPATEN JEMBER Utha, Maria Fransisca; Fany, Manta; Wahyukundari, Melok Aris; Pudjiastuti, Peni; Prasetya, Rendra C.; Harmono, Happy; Wanodyo, Ari Tri; Sari, Desi Sandra
Jurnal Kesehatan Vol 17 No 2 (2024): JURNAL KESEHATAN
Publisher : Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/kesehatan.v17i2.38943

Background: Periodontal disease is a dental and oral health problem that is often experienced by the elderly. Aging affects the ability of the elderly to carry out their daily activities, including cleaning the oral cavity. Increased accumulation of calculus due to poor oral hygiene can lead to the incidence of periodontal disease which affects the quality of life of the elderly. Purpose: to examine the status of oral hygiene and the need for periodontal tissue care in the elderly in the Pesisir Puger and Payangan area, Jember Regency. Methods: Descriptive observational with a cross-sectional approach. Subjects were selected as many as 192 elderly by purposive sampling technique. Data were obtained by taking questionnaires and clinical examinations in the form of OHI-S and CPITN. Results: the majority of elderly in the Pesisir Puger area of Jember district have a poor oral hygiene status of 96.88% and , the condition of the periodontal tissue has a 4-5 mm periodontal pocket of 66.67% and the need for periodontal tissue treatment is in the form of oral hygiene instructions and SRP procedures of 96.88%. Conclusion: Majority of the elderly in Pesisir Puger and Payangan have poor oral hygiene and require periodontal tissue treatment in the form of oral hygiene instructions and scaling and root planing procedures

Phytochemical properties and antibacterial activity of green tea leaf extract from gunung gambir jember against Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans: an experimental study Meilawaty, Zahara; Ermawati, Tantin; Sari, Desi Sandra; Ningsih, Indah Yulia; Rachmawati, Dessy; Nulend, Jenneke Klein
Padjadjaran Journal of Dentistry Vol 36, No 3 (2024): November 2024
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjd.vol36no3.58316

ABSTRACTIntroduction: Green tea leaves are abundant in bioactive components, including catechins, flavonoids, and polyphenols. These compounds are known for their antibacterial, anti-inflammatory, and antioxidant properties. In addition, green tea also contains minerals, such as Calcium, Phosphor, Potassium, Magnesium, and Sulphur. However, the precise mineral content and anti-bacterial properties of green tea against Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans from Gunung (Mount) Gambir in Jember Regency are currently unknown. This study aimed to analyze the phytochemical composition and antibacterial activity of green tea leaf extract from Gunung Gambir, Jember against Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans. Methods: The bioactive constituents of green tea leaf extract were identified using a UV-Vis spectrophotometer to measure its total flavonoid content (TFC) and total phenolic compounds (TPC). Mineral identification was performed using flame atomic absorption spectrometry. The disc diffusion method was used to assess antibacterial activity by quantifying the diameter of the inhibition zone in cultures of Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans treated with green tea leaf extract at concentrations of 3.125%, 6.25%, 12.5%, 25%, 50%, and 100%. Anaerobic conditions were created by incubating the Petri dishes in a desiccator for 24 hours at 37°C. One-Way Anova test was used to analyze the data, and differences were considered statistically significant at p<0.05. Subsequently, a LSD test was carried out (p<0,05). Results: The TPC value was 1.607±0.742 mg GAE/g, while the TFC value was 99.146 ± 0.307 mg GAE/g. Calcium appeared to be the most abundant mineral in the tea extract, measured at 31.65 ppm. The inhibitory power of green tea leaves extract against both bacteria was not significantly different (p>0.05). Conclusion: Green tea leaves extract from Gunung Gambir Jember Regency in Indonesia is rich in phenolic compounds, flavonoids, and certain minerals, such us Phosphor, Calium, Magnesium, Sulphur and Calcium. These constituents are known to possess biological and antibacterial activity against Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans.KEYWORDS Antibacterial, flavonoid, green tea extract, minerals, phenol, Jember

Peningkatan Pemanfaatan Tanaman Toga Penangkal Covid-19 Pada Kader Toga Puskesmas Kemuningsari Kidul Jember Desi Sandra Sari; Iis Rahmawati; Leersia Yusi
Dental Agromedis Vol. 2 No. 1 (2024): Mei
Publisher : Fakultas Kedokteran Gigi, Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/da.v2i1.1465

The problems of the community in the area of the Kemuningsari Kidul Public Health Center, Jenggawah, Jember are that they still do not use the yard with the toga plant optimally, the knowledge of the residents is still low, the lack of community resources and limited information and technology. During this pandemic, it is very important to maintain general health and increase body resistance to prevent the entry of the Covid-19 virus. This study aims to increase health knowledge, skills and use of the toga plant in the community independently through empowering toga cadres to behave in a healthy life during a pandemic in the Kemuningsari Kidul Public Health Center, Jenggawah, Jember. The solutions offered are counselling the benefits of the toga plant for endurance to prevent Covid-19 disease to toga cadres, public awareness through Toga cadres on the importance of maintaining body resistance by consuming the toga plant as a food supplement. Problem-solving from partners is carried out with a community service scheme. with the stages of providing counselling on the benefits of the Toga plant for endurance to prevent Covid 19 disease to Toga cadres, making the community aware through the Toga cadres of the importance of maintaining body resistance by consuming the Toga plant as an alternative to cheap, practical food supplements and training on how to use the Toga plant.Keywords: Toga Cadres, Immunity System, Covid-19, Kemuningsari Kidul Jember.

Analisis kandungan logam timbal (Pb) pada pasta gigi dengan ekstrak biji kopi robusta (Coffea canephora L.) Putri, Puspita Nabila; Pujiastuti, Peni; Sari, Desi Sandra
MKGK (Majalah Kedokteran Gigi Klinik) (Clinical Dental Journal) UGM Vol 11, No 2 (2025)
Publisher : Fakultas Kedokteran Gigi, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/mkgk.104299

Pasta gigi dengan ekstrak biji kopi robusta merupakan produk alternatif untuk mencegah penyakit periodontal yang mengandung bahan alami dengan sifat antibakteri, antiinflamasi, dan antioksidan. Pengembangan pasta gigi memerlukan pengujian untuk mendapatkan formula yang baik dan sesuai dengan Standar Nasional Indonesia (SNI). Salah satu pengujian yang perlu dilakukan adalah uji kandungan logam berat timbal (Pb). Tujuan penelitian ini adalah untuk mengetahui apakah kandungan Pb dalam pasta gigi dengan ekstrak biji kopi robusta sesuai dengan standar SNI. Ekstrak biji kopi robusta dibuat menggunakan metode maserasi dengan pelarut etanol 96%. Pasta gigi diformulasikan dengan mencampur pasta plasebo dan ekstrak biji kopi robusta pada konsentrasi 0,0625%, 0,125%, 0,25%, dan 0,5%. Pengujian kontaminasi Pb dilakukan menggunakan Spektrofotometer Serapan Atom (AAS) dengan hasil dinyatakan dalam mg/kg. Hasil penelitian menunjukkan bahwa ekstrak biji kopi robusta tidak mengandung Pb yang terdeteksi, dengan hasil tercatat sebagai "nd" atau "not detected", namun kandungan Pb sebesar 1,382mg/kg ditemukan pada pasta plasebo. Pada pasta gigi dengan ekstrak biji kopi robusta dengan berbagai konsentrasi, kandungan Pb berkisar antara 1,626 hingga 1,866mg/kg. Dari hasil penelitian ini dapat disimpulkan bahwa kandungan Pb dalam pasta gigi dengan ekstrak biji kopi robusta lebih rendah dari batas maksimum Pb yang ditetapkan SNI, yaitu kurang dari 20 mg/kg.

Karakterisasi bovine tooth scaffold dalam bentuk nanopartikel dengan uji x-ray diffraction (XRD) untuk terapi periodontitis Adisty, Anggi Salwa; Sari, Desi Sandra; Wahyukundari, Melok Aris
MKGK (Majalah Kedokteran Gigi Klinik) (Clinical Dental Journal) UGM Vol 11, No 2 (2025)
Publisher : Fakultas Kedokteran Gigi, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/mkgk.104301

Kerusakan tulang alveolar akibat periodontitis memiliki prevalensi tertinggi di Indonesia. Pencegahan kerusakan tulang alveolar dapat dilakukan dengan terapi regenerasi periodontal. Desain scaffold telah menjadi fokus utama untuk regenerasi jaringan tulang. Bovine teeth scaffold berukuran nanopartikel saat ini sedang dikembangkan sebagai solusi untuk mengatasi keterbatasan scaffold berukuran mikro. Scaffold harus memiliki karakteristik tertentu yang mendukung pertumbuhan dan diferensiasi sel untuk regenerasi jaringan periodontal yang dapat diketahui melalui uji karakterisasi X-ray diffraction. Penelitian ini bertujuan untuk mengetahui karakterisasi bovine teeth scaffold dalam bentuk nanopartikel melalui uji X-ray diffraction (XRD) untuk terapi periodontitis. Metode yang digunakan dalam penelitian ini adalah penelitian deskriptif kuantitatif untuk mendeskripsikan karakterisasi bovine teeth scaffold dalam bentuk nanopartikel dan data dianalisis secara deskriptif dengan tabel, kurva, dan nilai persentase. Hasil uji XRD menunjukkan bahwa terdapat dua fasa yang terdeteksi, yaitu hidroksiapatit dan whitlockite. Puncak-puncak difraksi hidroksiapatit muncul pada sudut difraksi 2θ sebesar 25,82˚; 31,67˚; 33,91˚; 49,33˚; 50,30˚ dengan puncak tertinggi pada sudut difraksi 31,67˚. Puncak-puncak difraksi whitlockite muncul pada sudut difraksi 2θ sebesar 28,86˚; 32,72˚; 39,58˚; 46,56˚ dengan puncak tertinggi pada sudut difraksi 32,72˚. Grafik menunjukkan pola difraksi sempit, tinggi, dan tajam yang mengindikasikan bahwa bovine teeth scaffold memiliki struktur kristal. Bovine teeth scaffold dalam bentuk nanopartikel mengandung 65% Hidroksiapatit (Ca10(PO4)6OH) dan 35% Whitlockite (Ca10NaMg(PO4)6(CO3) (OH)3). Karakterisasi bovine teeth scaffold dalam bentuk nanopartikel dengan menggunakan uji XRD diperoleh fasa hidroksiapatit sebesar 65% dan fasa whitlockite sebesar 35%.

EFFECTIVENESS OF ROBUSTA COFFEE BEAN EXTRACT GEL (Coffea canephora) ON THE THICKNESS OF COLLAGEN FIBERS AFTER WISTAR RAT TOOTH EXTRACTION Sari, Desi Sandra; Aisyah, Siti; Rachmawati, Dessy; Sutjiati, Rina; Yuwono, Budi
Odonto : Dental Journal Vol 10, No 2 (2023): December 2023
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar

Background: Tooth extraction causes injury to the alveolar bone and oral mucosa which is followed by the body â€™s natural response through wound healing. One of the important parameters and indicators of wound healing is collagen density. Collagen is synthesised by fibroblasts in the proliferative phase, which was formed from day 3 and reached its peak on day 7. Robusta coffee beans have active compounds in the form of anti-inflammatory and antioxidants that are effective in accelerating wound healing. Objective: To determine the effectiveness of robusta coffee bean extract gel (Coffea canephora) at concentrations of 12,5%, 25%, and 50% in increasing the thickness of collagen fibres in socket wound healing after tooth extraction in Wistar rats. Method: This type of research is laboratory experimental. The samples used were 40 male Wistar rats divided into 5 groups: negative control, positive control, 12.5% robusta coffee bean extract gel treatment, 25% robusta coffee bean extract gel treatment, and 50% robusta coffee bean extract gel treatment. The sample group was decapitated on the 3rd and 7th day after tooth extraction. Socket tissues were taken to make histological preparations stained with trichrome mallory and then measured using Adobe Photoshop CS 6.0 software. Results: The average thickness of collagen fibres in the Robusta coffee bean extract gel treatment group was higher than that in the control group (p

EFFECTIVENESS OF ROBUSTA COFFEE BEAN EXTRACT GEL (Coffea canephora) ON THE THICKNESS OF COLLAGEN FIBERS AFTER WISTAR RAT TOOTH EXTRACTION Sari, Desi Sandra; Aisyah, Siti; Rachmawati, Dessy; Sutjiati, Rina; Yuwono, Budi
Odonto : Dental Journal Vol 10, No 2 (2023): December 2023
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar

Background: Tooth extraction causes injury to the alveolar bone and oral mucosa which is followed by the body â€™s natural response through wound healing. One of the important parameters and indicators of wound healing is collagen density. Collagen is synthesised by fibroblasts in the proliferative phase, which was formed from day 3 and reached its peak on day 7. Robusta coffee beans have active compounds in the form of anti-inflammatory and antioxidants that are effective in accelerating wound healing. Objective: To determine the effectiveness of robusta coffee bean extract gel (Coffea canephora) at concentrations of 12,5%, 25%, and 50% in increasing the thickness of collagen fibres in socket wound healing after tooth extraction in Wistar rats. Method: This type of research is laboratory experimental. The samples used were 40 male Wistar rats divided into 5 groups: negative control, positive control, 12.5% robusta coffee bean extract gel treatment, 25% robusta coffee bean extract gel treatment, and 50% robusta coffee bean extract gel treatment. The sample group was decapitated on the 3rd and 7th day after tooth extraction. Socket tissues were taken to make histological preparations stained with trichrome mallory and then measured using Adobe Photoshop CS 6.0 software. Results: The average thickness of collagen fibres in the Robusta coffee bean extract gel treatment group was higher than that in the control group (p

The effect of supernatants of Lactobacillus casei against Porphyromonas gingivalis Zainina, Iris Amelinda; Kusumawardani, Banun; Sari, Desi Sandra
Majalah Kedokteran Gigi Indonesia Vol 11, No 2 (2025): August
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/majkedgiind.101506

Periodontitis is an inflammatory disease of the periodontal tissues primarily caused by Porphyromonas gingivalis (P. gingivalis). In recent years, probiotics have been proposed as a potential bacteriotherapeutic approach for periodontitis. Probiotic bacteria such as Lactobacillus casei (L. casei) are known to produce antibacterial compounds, which can be found in their culture supernatants. The ability of L. casei to grow and synthesize these substances depends on environmental factors, including pH. Based on preliminary findings, a pH of 6.5 was used in this study. This study aimed to evaluate the antibacterial activity of L. casei supernatant against P. gingivalis in vitro. The research employed an experimental post-test-only group design. Two control groups were included: a negative control (aquadest) and a positive control (0.2% chlorhexidine). The treatment groups received L. casei supernatant at volumes of 20 µl, 50 µl, and 80 µl, with incubation periods of 6, 12, and 24 hours. The results demonstrated that the L. casei supernatant exhibited the highest antibacterial activity against P. gingivalis at a volume of 80 µl after 12 hours of incubation. The Shapiro–Wilk test indicated that the data were normally distributed, while Levene’s test revealed a lack of homogeneity. Post hoc analysis showed significant differences among all treatment groups at the 12-hour incubation point. In conclusion, the supernatant of L. casei exhibits in vitro antibacterial activity against P. gingivalis.

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