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All Journal AGRITROP Buletin Udayana Mengabdi Majalah Ilmiah Peternakan Pastura; Journal Of Tropical Forage Science Jurnal Peternakan Tropika PTK: Jurnal Tindakan Kelas
I WAYAN SUBERATA
Fakultas Peternakan Universitas Udayana, Denpasar Bali
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Veterinary Other

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Journal : AGRITROP

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TRANSFORMASI GEN ALBUMIN BUNGA MATAHARI (HELIANTHUS ANNUUS L.) PADA TANAMAN KEDELAI MENGGUNAKAN VEKTOR AGROBAKTERIUM TUMEFACIENS LBA4404 (PAL4404, PSW600) SECARA IN VITRO SUBERATA, I WAYAN
AGRITROP Vol. 26, No. 2 Juni 2007
Publisher : AGRITROP

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Abstract

Usages of A.tumefaciens for the purpose of including DNA sunflower albumine into soybean cell, constituted of natural ability of A. tumefaciens to trasformation in specific form of DNA plasmid (T-DNA) into crop cell later, and then integrate at mains crop cell genome. Result of gene cloning of albumine grown at bacterium of E.coli, then carried over by A. tumefaciens LBA4404, passing method of triparental mating. A. tumefaciens LBA4404 (pAL4404, pSW600) yielded later, then transformation at soybean by in vitro. Result of analysis of transformation at soybean of transgenic by in vitro, can be proved with testing its resilience to gene activity of GUS-its and canamisin.
Co-Authors A. W. Puger Adi Parwata I G. B. Anak Agung Putu Putra Wibawa ARTININGSIH NM Aslamiyah, Ditya Banurea M.R DARSONO I Dewa Ketut Harya Putra I Gusti Agung Arta Putra I Gusti Ngurah Kayana I Ketut Sukada I Komang Budaarsa I M. Mudita I M. Mudita I N. S. Sutama I.G.E. Putra I.M. Nitis I.M. Suarn IK Sukada IN Ardika IN. S Sutama IW Wijana N. M. ARTININGSIH Ni Luh Gde Sumardani Ni Made Artiningsih Rasna Suranjaya I .Gd Wayan Sayang Yupardhi Wirawan I W.