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All Journal JSME MIPA UNIMA JURNAL PENDIDIKAN TAMBUSAI Vivabio : Jurnal Pengabdian Multidisiplin Jurnal Agroekoteknologi Terapan Indonesian Biodiversity Journal Indonesian Biodiversity Journal
Utari Satiman
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Education Environmental Science Immunology & microbiology Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Public Health Social Sciences Veterinary

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CHARACTERIZATION OF SAPROPHAGE INSECTS FORENSIC ENTOMOLOGY INDICATORS USING LOCAL PIGS Kandowangko, Sandy; Wurarah, Masje; Mokosuli, Yermia; Satiman, Utari; Gedoan, Sukmarayu
Indonesian Biodiversity Journal Vol. 5 No. 2 (2024): August 2024
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v5i2.10755

Abstract

Forensic entomology is the science of insects which is used to analyze cases related to forensics, namely death. This is based on the close relationship between humans and insects who live side by side. Insects can be a factor in determining the Postmortem Interval (PMI) so that it is useful in the forensic investigation process. Medicolegal insects are insects that are used as evidence in death cases. This research aims to obtain the diversity of insect species, the morphological characteristics of insects, the characteristics of insects in experimental pigs that died from hanging, poisoning and stabbing and based on the stage of decomposition. The results of this research examine temperature, soil pH, air humidity and the decomposition process of local pigs. The results of research on the decomposition stage in 3 deaths (hanging, stabbing, and poisoning) showed differences in the time of death, where the hanging carcass decomposed more quickly than the stabbed and poisoned carcass. In the decomposition stage, the first insect encountered is the order (Diptera) where these insects damage internal organs such as the brain and liver and also damage body cells. The temperature results of local pig carcasses placed outdoors ranged from 32.2-34.50C, while indoor carcass temperatures ranged from 26.77-33.43 0C. The degree of acidity (pH) of carcasses placed outdoors ranges from 5.13-8.53 while indoor carcasses ranges from 6.0-7.77. The environmental temperature of animal bodies placed outdoors ranges from 26.35-39,740C, while humidity ranges from 57.79-86.10%. The temperature of animal bodies in the room ranges from 27.54-31,520C, while humidity ranges from 71.50-85.37%.
Growth and Yield of Lettuce Plants (Lactuca sativa L.) with Alternative Nutrition to Subtitute AB Mix in Hydroponic Systems Toheni, Fredrik; Mege, Revolson; Satiman, Utari
Indonesian Biodiversity Journal Vol. 5 No. 1 (2024): April 2024
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v5i1.9075

Abstract

The hydroponic system is modern agricultural cultivation with various planting media as a substitute for soil, so hydroponics is an agricultural activity that is carried out by using water as a medium for distributing nutrients. This research aims to determine the effect of alternative nutrition to substitute AB mix on the growth and yield of hydroponic lettuce (Lactuca sativa L.). The research method was an experiment in a completely randomized design(CRD), using 5treatments with 4 repetitions, namely P0 (control/with ABmix nutrition) of 700-800 ppm, P1 (800-900 alternative nutrients), P2 (900-1000 ppm alternative nutrients), P3 (1000-1100 alternative nutrients), P4 (1000-1200 alternative nutrients), were analyzed by one way ANOVA analysis with a significance level of 0.05. Parameters observed were plant height, number of leaves, root length, leaf width, and fresh weight. The results showed that providing alternative nutrition significantly affected plant height, leaf width, and fresh weight of lettuce (Lactuca sativa L.). With the best treatment of 900-1000 ppm with plant height 35-41 cm, number of leaves 15-17, leaf width 17-18 cm, and plant wet weight 120-209 grams, the lowest yield was in treatment P0 with plant height 30-33 cm. the number of leaves is 15-16, and the wet weight of the plant is 117-146 grams
CHARACTERIZATION OF SAPROPHAGE INSECTS FORENSIC ENTOMOLOGY INDICATORS USING LOCAL PIGS Kandowangko, Sandy; Wurarah, Masje; Mokosuli, Yermia; Satiman, Utari; Gedoan, Sukmarayu
Indonesian Biodiversity Journal Vol. 5 No. 2 (2024): August 2024
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v5i2.10755

Abstract

Forensic entomology is the science of insects which is used to analyze cases related to forensics, namely death. This is based on the close relationship between humans and insects who live side by side. Insects can be a factor in determining the Postmortem Interval (PMI) so that it is useful in the forensic investigation process. Medicolegal insects are insects that are used as evidence in death cases. This research aims to obtain the diversity of insect species, the morphological characteristics of insects, the characteristics of insects in experimental pigs that died from hanging, poisoning and stabbing and based on the stage of decomposition. The results of this research examine temperature, soil pH, air humidity and the decomposition process of local pigs. The results of research on the decomposition stage in 3 deaths (hanging, stabbing, and poisoning) showed differences in the time of death, where the hanging carcass decomposed more quickly than the stabbed and poisoned carcass. In the decomposition stage, the first insect encountered is the order (Diptera) where these insects damage internal organs such as the brain and liver and also damage body cells. The temperature results of local pig carcasses placed outdoors ranged from 32.2-34.50C, while indoor carcass temperatures ranged from 26.77-33.43 0C. The degree of acidity (pH) of carcasses placed outdoors ranges from 5.13-8.53 while indoor carcasses ranges from 6.0-7.77. The environmental temperature of animal bodies placed outdoors ranges from 26.35-39,740C, while humidity ranges from 57.79-86.10%. The temperature of animal bodies in the room ranges from 27.54-31,520C, while humidity ranges from 71.50-85.37%.
Pemberdayaan Masyarakat Tani Desa Kayuuwi Kecamatan Kawangkoan Tentang Pembuatan Pupuk Organik dari Limbah Pertanian dan Rumah Tangga : Community Empowerment of Kayuuwi Village Farmers, Kawangkoan District Regarding Organic Fertilizer Based on Agricultural and Household Waste Satiman, Utari; Rungkat, Jouveline; Batmetan, Johan; Taulu, Marthy
Vivabio: Jurnal Pengabdian Multidisiplin Vol. 7 No. 2 (2025): VIVABIO: Jurnal Pengabdian Multidisiplin
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/vivabio.v7i2.62930

Abstract

The Community Service activity partners are the Farmer Community, who are members of the Berkat Kayuuwi Kawangkoan Minahasa farmer group, with the following problems faced by the partners: 1) Low knowledge of farmer group members about organic fertiliser. 2) Low skills of farmers in empowering agricultural waste and household waste into organic fertiliser. 3). Low knowledge and skills of farmers in innovating daily community life activities in Kayuuwi Village, Kawangkoan District, such as household activities, farming, livestock raising which are found to have much waste that can be used as raw materials for making organic fertiliser so that it can increase community income while improving the quality of the soil and the surrounding environment. The methods used to achieve the PKM objectives were carried out in stages and systematically, starting with outreach and training. Then, direct field assistance until all activities were completed and partner issues were resolved. In addition to theoretical training, participants were also involved in hands-on practice in making solid compost. They were taught how to use a simple shredder as part of the application of appropriate technology. Evaluation was conducted by comparing pre-test and post-test results to assess participants' knowledge gains. The evaluation results showed a significant increase in farmers' understanding and skills, even reaching a 300% increase in some topics. Furthermore, organic fertiliser produced from agricultural and household waste was also directly utilised in peanut cultivation activities by farmer groups. This activity was not only educational but also productive because it encouraged farmers to be independent in meeting their fertiliser needs and opened up small business opportunities in organic farming. ABSTRAK Mitra kegiatan Pengabdian Kepada Masyarakat (PKM) adalah Masyarakat Tani yang tergabung dalam kelompok tani Berkat Kayuuwi Kawangkoan Minahasa dengan permasalahan yang dihadapi mitra sebagai berikut: 1). Rendahnya pengetahuan anggota kelompok tani tentang pupuk organik. 2). Rendahnya keterampilan petani  dalam memberdayakan limbah pertanian dan limbah rumah tangga menjadi pupuk organik. 3). Rendahnya pengetahuan dan keterampilan petani dalam menginovasi aktifitas kehidupan masyarakat sehari-hari di Desa kayuuwi Kecamatan Kawangkoan, seperti aktivitas Rumah tangga, bercocok tanam, beternak yang didapati banyak limbahnya yang bisa dimanfaatkan menjadi bahan baku pembuatan pupuk organik sehingga bisa menambah pendapatan masyarakat sekaligus memperbaiki kualitas tanah dan lingkungan sekitar.  Metode yang digunakan untuk mencapai tujuan PKM dilakukan secara bertahap dan sistematis dimulai dari sosialisasi, pelatihan, hingga pendampingan langsung di lapangan sampai seluruh kegiatan selesai dan permasalahan mitra teratasi.  Selain pelatihan teori, peserta juga dilibatkan dalam praktek langsung pembuatan pupuk kompos padat. Mereka diajarkan menggunakan mesin pencacah sederhana sebagai bagian dari penerapan teknologi tepat guna. Evaluasi dilakukan dengan membandingkan hasil pre-test dan post-test untuk menilai peningkatan pengetahuan peserta. Hasil evaluasi menunjukkan adanya peningkatan signifikan dalam pemahaman dan keterampilan petani, bahkan pada beberapa topik mencapai kenaikan hingga 300%. Tidak hanya itu, pupuk organik yang dihasilkan dari limbah pertanian dan rumah tangga juga langsung dimanfaatkan dalam kegiatan budidaya kacang tanah oleh kelompok tani. Kegiatan ini tidak hanya bersifat edukatif tetapi juga produktif karena mampu mendorong petani untuk mandiri dalam memenuhi kebutuhan pupuk serta membuka peluang usaha kecil di bidang pertanian organik.    
Incidence of Yellow Virus Disease in Chili Pepper Plants (Capsicum frutescens L.) in East Tombatu District, Southeast Minahasa Regency Pinaria, Arthur Gehart; Rondonuwu, Frans Bernhard; Satiman, Utari
Jurnal Agroekoteknologi Terapan (Applied Agroecotechnology Journal) Vol. 6 No. 2 (2025): ISSUE JULY-DECEMBER 2025
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35791/jat.v6i2.65949

Abstract

The purpose of this study is to ascertain the incidence of diseases in the chili plant population in East Tombatu District, Southeast Minahasa Regency, caused by the yellow virus that affects plants in terms of area. The sample garden is at least half a hectare in size. Purposive sampling was used to choose the sample gardens, and the survey was only done once. Five subplots, one in the center of the garden and one close to each corner, were used for sampling in each chili garden (plot). The quantity of individual plants, or sample units, in each subplot. The number of samples represents 20% of the total number of plants. The findings revealed that the melompar atas garden had the highest prevalence of yellow viral disease in cayenne pepper (100%), followed by Mundung (180.91%), esandom (80%), Malam part 2 (8.80%), and jumping (141.88%). The incidence of this illness rose as the plant grew older after being planted. Further study ideas include inventorying B. tabachi hosts, inventorying gemini virus hosts, and applying natural farming to reduce cayenne pepper infections. Keywords: Yellow Virus, Incidence, Capsicum frutescens. Abstrak. Penelitian ini bertujuan untuk mengetahui keseringan penyakit yang disebabkan oleh virus kuning yang menyerang tanaman dalam aspek luasan pada populasi tanaman cabai di Kecamatan Tombatu Timur, Kabupaten Minahasa Tenggara. Kebun sampel berukuran minimal 0,5 ha. Pemilihan kebun sampel dilakukan secara purposive sampling, dan dan survei hanya dilakukan satu kali. Sampling pada setiap kebun (plot) cabe dilakukan pada lima subplot (setiap dekat sudut kebun satu plot dan satu subplot di tengah kebun).  Jumlah unit contoh (individu tanaman) per subplot. Jumlah  sampel ialah 20 % dari jumlah tanaman. Hasil penelitian menunjukkan bahwa insidensi penyakit virus kuning pada cabe rawit tertinggi terdapat di kebun melompar atas 100% diikuti oleh Mundung 180,91% esandom 80% Malam part 2 6 8,80% dan melompat 141,88% insidensi Penyakit ini makin tinggi seiring dengan makin tingginya umur tanaman sesudah tanam. Penelitian lanjutan yang disarankan ialah: inventarisasi inang-inang B. tabachi, menginventarisasi inang-inang gemini virus dan penerapan natural farming untuk mengendalikan patogen cabe rawit secara sekaligus. Kata-Kata Kunci: Virus Kuning, Insidensi, Capsicum frutescens
Lactic Acid Bacteria That Produce Antibacterial Compunds on Candied Pakoba Fruit Syzygium luzonense Runtuwene, Kharly; Lawalata, Helen; Posumah, Dany; Satiman, Utari; Moko, Emma
Indonesian Biodiversity Journal Vol. 6 No. 2 (2025): August 2025
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/srqbqw88

Abstract

Lactic Acid Bacteria (LAB) are a group of Gram-positive, cocci or rod-shaped microorganisms characterized by their catalase-negative phenotype. These bacteria are known to produce various antibacterial compounds, including organic acids, hydrogen peroxide, carbon dioxide, and bacteriocins, which exhibit bacteriostatic or bactericidal properties against pathogenic bacteria. The aim of this study is to isolate LAB that produce antibacterial compounds during the fermentation of Pakoba fruit (Syzygium luzonense) into sweets, with a focus on inhibiting the growth of Escherichia coli and Staphylococcus aureus. This research employs a quantitative descriptive method, involving the isolation of LAB from candied Pakoba fruit samples and subsequent testing of their antibacterial activity against the aforementioned pathogens. The barrier zone formed around the wells is then measured. The data from the study showed that BAL isolation from Pakoba fruit candied was obtained from as many as 10 isolates, and antibacterial activity tests showed that 10 BAL isolates produced antibacterial compounds with inhibitory diameters of 7.6 – 15.3 mm. The isolates with the greatest antibacterial activity are  LAB isolate MP(1)5.2  for E. coli and LAB isolate MP(3)6.3 isolates for S. aureus. Based on the results of identification using the profile matching method, it was shown that the selected LAB isolates, namely MP(1)5.2 and MP(3)6.3, were classified in the genus Lactobacillus with the characteristics of stem cell shape, single cell arrangement, catalase-negative, non-motile, did not form spots, and did not produce gas from glucose.
THE EFFECT OF THE USE OF CHICKEN MANURE ON RED BEANS (Phaseolus vulgaris L.) Goo, Alince; Satiman, Utari; Lawalata, Helen; Tumbel, Ferny Margo; Wantania, Johanna
Indonesian Biodiversity Journal Vol. 6 No. 2 (2025): August 2025
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/dc0z3470

Abstract

Red beans are a horticultural crop belonging to the annual legume group that grows upright and has a fairly high nutritional content. To obtain maximum growth and yield from red beans, several factors must be considered, including balanced fertilizer application. The factor of providing organic fertilizer from chicken manure with the right dosage is the target of this study. This study aims to determine the effect of red bean growth on the dosage of chicken manure fertilizer. This study was conducted from December 2024 to February 2025 and was carried out on the plantation land of the Department of Science Education, Faculty of Mathematics, Natural Sciences and Earth Sciences, Manado State University. The method used is a quantitative method with a type of experiment in the form of a Randomized Block Design (RAK) with five treatment combinations with each repeated three times. A0 = 0 (control), A1 = 1.00 kg, A2 = 1.50 kg, A3 = 2.00 kg, A4 = 2.50 kg, so there are 15 experimental bed units, and the data obtained are then analyzed using analysis of variance (ANOVA). The results of this study indicate that good plant height growth, number of leaves and stem diameter are found in treatment A1 with a dose of 1.00 kg of chicken manure fertilizer and A2 with a dose of 1.50 kg of chicken manure fertilizer because there is the most maximum growth.
ETHNOBOTANICAL STUDY OF TRADITIONAL MEDICINAL PLANTS AND THEIR UTILIZATION IN PASSI VILLAGE, WEST PASSI DISTRICT, BOLAANG MONGONDOW REGENCY Latip, Irwan; Satiman, Utari; Djarang, Rievo
Indonesian Biodiversity Journal Vol. 6 No. 2 (2025): August 2025
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/k8p6pd73

Abstract

Ethnobotany is the study of the relationship between plants and humans, including the use of plants by local communities. Traditional medicinal plants are plants that are often used by local communities for their health benefits. This study aims to describe the types of medicinal plants used and how they are processed using traditional methods in the village of Passi, West Passi subdistrict, Bolaang Mongondow district. This research is descriptive qualitative with an interview method and descriptive analysis technique. The results of this study show that there are 37 types of medicinal plants used with a total of 23 families found. The methods of processing traditional medicinal plants are: boiling, squeezing, soaking, pounding, and not processing. The most commonly used processing method is boiling, with 24 types of medicinal plants using this method. The parts of the plants that are most commonly used are: stems, fruits, flowers, leaves, roots, gel, sap, rhizomes, and tubers. The most commonly used parts are leaves, rhizomes, and fruits.
Lactic Acid Bacteria That Produce Antibacterial Compunds on Candied Pakoba Fruit Syzygium luzonense Runtuwene, Kharly; Lawalata, Helen; Posumah, Dany; Satiman, Utari; Moko, Emma
Indonesian Biodiversity Journal Vol. 6 No. 2 (2025): August 2025
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/srqbqw88

Abstract

Lactic Acid Bacteria (LAB) are a group of Gram-positive, cocci or rod-shaped microorganisms characterized by their catalase-negative phenotype. These bacteria are known to produce various antibacterial compounds, including organic acids, hydrogen peroxide, carbon dioxide, and bacteriocins, which exhibit bacteriostatic or bactericidal properties against pathogenic bacteria. The aim of this study is to isolate LAB that produce antibacterial compounds during the fermentation of Pakoba fruit (Syzygium luzonense) into sweets, with a focus on inhibiting the growth of Escherichia coli and Staphylococcus aureus. This research employs a quantitative descriptive method, involving the isolation of LAB from candied Pakoba fruit samples and subsequent testing of their antibacterial activity against the aforementioned pathogens. The barrier zone formed around the wells is then measured. The data from the study showed that BAL isolation from Pakoba fruit candied was obtained from as many as 10 isolates, and antibacterial activity tests showed that 10 BAL isolates produced antibacterial compounds with inhibitory diameters of 7.6 – 15.3 mm. The isolates with the greatest antibacterial activity are  LAB isolate MP(1)5.2  for E. coli and LAB isolate MP(3)6.3 isolates for S. aureus. Based on the results of identification using the profile matching method, it was shown that the selected LAB isolates, namely MP(1)5.2 and MP(3)6.3, were classified in the genus Lactobacillus with the characteristics of stem cell shape, single cell arrangement, catalase-negative, non-motile, did not form spots, and did not produce gas from glucose.
THE EFFECT OF THE USE OF CHICKEN MANURE ON RED BEANS (Phaseolus vulgaris L.) Goo, Alince; Satiman, Utari; Lawalata, Helen; Tumbel, Ferny Margo; Wantania, Johanna
Indonesian Biodiversity Journal Vol. 6 No. 2 (2025): August 2025
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/dc0z3470

Abstract

Red beans are a horticultural crop belonging to the annual legume group that grows upright and has a fairly high nutritional content. To obtain maximum growth and yield from red beans, several factors must be considered, including balanced fertilizer application. The factor of providing organic fertilizer from chicken manure with the right dosage is the target of this study. This study aims to determine the effect of red bean growth on the dosage of chicken manure fertilizer. This study was conducted from December 2024 to February 2025 and was carried out on the plantation land of the Department of Science Education, Faculty of Mathematics, Natural Sciences and Earth Sciences, Manado State University. The method used is a quantitative method with a type of experiment in the form of a Randomized Block Design (RAK) with five treatment combinations with each repeated three times. A0 = 0 (control), A1 = 1.00 kg, A2 = 1.50 kg, A3 = 2.00 kg, A4 = 2.50 kg, so there are 15 experimental bed units, and the data obtained are then analyzed using analysis of variance (ANOVA). The results of this study indicate that good plant height growth, number of leaves and stem diameter are found in treatment A1 with a dose of 1.00 kg of chicken manure fertilizer and A2 with a dose of 1.50 kg of chicken manure fertilizer because there is the most maximum growth.
Co-Authors Arrijani - Bandu, Julandi Batmetan, Johan Dany Posumah Djarang, Rievo Dumgair, Sarah Ferni Margo Tumbel Goo, Alince Herry Maurits Sumampouw Kandowangko, Sandy Latip, Irwan Lawalata, Helen Marthy L S Taulu Masje Wurarah Masye Wurarah Moko, Emma Mokosuli Yermia Samuel Ogi, Ceacillia Orbanus Naharia Pinaria, Arthur Gehart Revolson Mege Rondonuwu, Frans Bernhard Rungkat, Jouveline Runtuwene, Kharly Simarmata, Iyusnita Sari Sukmarayu P. Gedoan Tahulending, Atika Toheni, Fredrik Tumbel, Ferny M. Unaola, Valerika Wantania, Johanna Worang, Rantje L.