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PENGARUH KADAR RESIQUIMOD (R848) TERHADAP PERSENTASE SPERMATOZOA X DAN Y PADA SEMEN KAMBING PERANAKAN ETAWAH NURHAYATI, SITI ZYA; Solihati, Nurcholidah; Setiawan, Rangga
Jurnal Produksi Ternak Terapan Vol 6, No 1 (2025): Volume 6 Nomor 1 Maret 2025
Publisher : Faculty of Animal Husbandry, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/jptt.v6i1.55886

Abstract

The swim up method is a method of selecting X and Y spermatozoa. This study aims to determine how resiquimod (R848) levels affect the percentage of X spermatozoa and Y spermatozoa in the semen of Etawah crossbreed goats (PE) resulting from sexing using the swim up method. This research was carried out from 1 - 30 January 2024 with the research object being male PE goats with a lifespan of 4 years. The research results were analyzed using variance, followed by Duncan's multiple range test to determine differences between treatments. In this study, four treatments were given, namely  (without R848),  (R484 0.3 μM),   (R484 0.6 μM), and  (R848 0.9 μM). Each treatment was repeated six times. The results of this study showed that the use of resiquimod (R848) did not have a significant difference in the percentage of X and Y spermatozoa. It can be concluded that it had no effect on the percentage of PE goat spermatozoa in the lower and upper layers.Keywords: Resiquimod (R848), Percentage of Spermatozoa, Swim up method, Goat PE
The Effect Of Antioxidant (Alpha-Tocopherol) Addition In Preservation Medium On Follicle Diameter And Oocyte Quality After Local Sheep Ovary Preservation Faradana, Najma Rika; Widyastuti, Rini; Setiawan, Rangga
JURNAL ILMIAH PETERNAKAN TERPADU Vol. 13 No. 3 (2025)
Publisher : DEPARTMENT OF ANIMAL HUSBANDRY, FACULTY OF AGRICULTURE, UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jipt.v13i3.p702-714

Abstract

This study aimed to evaluate the effect of adding the antioxidant Alpha-Tocopherol to the preservation medium on follicle diameter and oocyte quality of local sheep ovaries after 24 hours of storage at 4–5°C. The ovaries were divided into four treatment groups: P1 (dPBS), P2 (dPBS + 200 µM H₂O₂), P3 (dPBS + 200 µM H₂O₂ + 3 mM alpha-tocopherol), and P4 (dPBS + 3 mM alpha-tocopherol). The results showed no significant differences in follicle diameter changes among all treatments. The addition of Alpha-Tocopherol also did not demonstrate a notable effect in maintaining oocyte quality. Oocytes collected from all treatment groups exhibited relatively similar percentages of grade 1, 2, and 3. This study indicates that Alpha-Tocopherol does not provide a significant protective effect on follicle diameter or oocyte quality during short-term ovary preservation.
Penyuluhan Komunikasi Bekal Gizi Seimbang antara Ibu dan Anak di Kelurahan Keranggan Octory, Gadis; Fitriani, Resa; Ulfie, Nasya Shalsabiel; Shabrina, Tiara; Ayu Putri Noviana, Ninda; Dwidika, Adyatma; Setiawan, Rangga
Jurnal Pengabdian Masyarakat Indonesia Vol 4 No 4 (2024): JPMI - Agustus 2024
Publisher : CV Infinite Corporation

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52436/1.jpmi.2753

Abstract

Gizi seimbang merupakan aspek penting dalam menunjang kesehatan dan kesejahteraan anak-anak di Indonesia. Penelitian ini bertujuan untuk meningkatkan pengetahuan dan kreativitas ibu-ibu dalam mengkomunikailan gizi seimbang kepada anak-anak di Kelurahan Keranggan, Tangerang Selatan. Metode yang digunakan adalah observasi, wawancara, workshop, dokumentasi, dan triangulasi data. Hasil penelitian menunjukkan bahwa program penyuluhan komunikasi bekal gizi seimbang efektif dalam meningkatkan pengetahuan dan kreativitas ibu-ibu. Sebagian besar ibu setelah mengikuti program workshop menilai bahwa program dan materi yang disuguhkan sangat penting dan menarik. Hal ini dapat dilihat pada Tabel Rekapitulasi data. Penelitian ini merekomendasikan program penyuluhan serupa untuk dilakukan di wilayah lain dengan fokus pada peningkatan pengetahuan dan praktik kreativitas ibu-ibu dalam mengkomunikailan bekal gizi seimbang kepada anak-anak.
IMPROVING EWE OOCYTE VIABILITY AFTER VITRIFICATION WARMING USING COMBINATION OF DIFFERENT CRYOPROTECTANT AND CARRIER SYSTEM Widyastuti, Rini; Setiawan, Rangga; Syamsunarno, Mas Rizky Anggun Adipurna; Ghozali, Mohammad; Saili, Takdir; Boediono, Arief
Jurnal Kedokteran Hewan Vol 12, No 3 (2018): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v12i3.11398

Abstract

The aim of this study was to determine the best combination of cryoprotectant (Ethylene glycol, EG), dimethyl sulfoxide (DMSO) and propanediol (PrOH) and carrier system (hemistraw and cryotop) in improving ewe oocytes viability during cryopreservation. Oocytes with multi layers of compact cumulus cells were colleted from abbatoir and matured in TCM 199 medium supplemented with 10% fetal calf serum for 24-26 h at 38.5 C under 5% CO2 in the air. Matured oocyte was divided into six parts and vitrified in three different vitrification solutions; (i) 17% EG+17% DMSO with hemistraw as carrier system, (ii) 34% EG with hemistraw as carrier system, (iii) 17% EG+17% PrOH in hemistraw (iv), 17% EG+17% DMSO with cryotop as carrier system (v), 34% EG with cryotop as carrier system (vi), and 17% EG+17% PrOH in cryotop. Oocytes were cryopreserved for one week before revived and evaluated for viability. The result showed that oocytes vitrified in media containing EG and DMSO in cryotop had the highest viability (88.16%) compared to media containing EG only or EG and PrOH (70.95% and 68.76%, respectively) (P0.05). Moreover, oocytes viability that vitrified using cryotop and hemistraw as carrier system were not significantly different. The present results indicated that vitrification using combination of EG and DMSO as permeable cryoprotectant and cryotop as carrier system was the best system to maintain oocyte viability after vitrification-warming.
Effect of PMSG administration in combination with vaginal sponge on estrous occurrence and litter size of Javanese sheep Setiawan, Rangga; Rasad, Siti Darodjah; Solihati, Nurcholidah; Widyastuti, Rini; Soeparna, Soeparna
Jurnal Kedokteran Hewan Vol 11, No 4 (2017): December
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v11i4.4349

Abstract

AbstractThe use of vaginal sponge in estrous synchronization has been a popular method to uniform estrous cycle of small ruminants. In order to increase the litter size, the use of the sponge is combined with PMSG. Therefore the objective of this study was to evaluate the effect of PMSG in combination with vaginal sponge on estrous response and litter size of Javanese local sheep. The study was conducted in Faculty of Animal Husbandry, Padjadjaran University, Indonesia with 32 non-pregnant local sheep. All these sheep was divided into four groups of treatment, i.e: 1) group of sheep was subjected to vaginal sponge (VS) treatment containing 30 mg Medroxyprogesterone acetat (MAP) inserted in the vagina for 14 days; 2) group of sheep was subjected to the 30 mg MAP in VS plus 300 IU PMSG injection -1 d before VS removal; 3) group of sheep was subjected to the 30 mg MAP in VS plus 300 IU PMSG injection at the day of VS removal; 4) group of sheep was subjected to the 30 mg MAP in VS plus 300 IU PMSG injection +1 d after VS removal. The result showed that all treatment groups had 100% of estrous response. However, the occurrence of estrous time varied among group after VS removal. Group 1 (G1) and G4 tend to have estrous occurrence at day 3 after VS removal by 55% and 75% response, respectively. Whilst, G2 and G3 tend to have estrous occurrence respectively at day 1 (62,5%) and day 2 (50%) after VS removal. A 100% of pregnancy rate occurs in G2 and G4, while G1 and G3 have a 88,9% and 87,5%, respectively. No significant effects of the treatments on the ewes litter size. In conclusion, the use of PMSG administration 24 h prior, after, or at sponge withdrawal increase a tighter synchrony of oestrous and pregnancy rate, but has no effect on the increasing of litter size in Javanese local sheep.Keywords: Vaginal sponge, PMSG, estrous response, pregnancy rate, litter size, sheep
Pengaruh kombinasi pH media dan lama inkubasi terhadap ukuran panjang, lebar, dan luas kepala sperma hasil sexing Ibrahim, M. Rangga Rangga Katar; Solihati, Nurcholidah; Setiawan, Rangga
Jurnal Produksi Ternak Terapan Vol 6, No 3 (2025): Volume 6 Nomor 3 November 2025
Publisher : Faculty of Animal Husbandry, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/jptt.v6i3.65924

Abstract

Sexing sperma metode swim up merupakan proses pemisahan spermatozoa X danY yang paling mudah dan ekonomis. Penelitian ini bertujuan untuk mengetahui pengaruh kombinasi pH media dan lama inkubasi terhadap morfometri sperma pembawa kromosom X-Y pada kambing sapera hasil sexing. Penelitian ini menggunakan Rancangan Acak Lengkap dengan enam perlakuan kombinasi pH dan lama inkubasi, yaitu P1 (6,2-40), P2 (6,8-40), P3 (7,4-40), P4 (6,2-60), P5 (6,8-60), P6 (7,4-60), setiap perlakuan diulang 5 kali. Data penelitian dianalisis menggunakan analisis varian dengan software statplus. Hasil penelitian menunjukkan rataan minimal dan maksimal ukuran panjang 7,49 ± 0,05 dan 10,03 ± 0,11 µm, serta lebar 3,54 ± 0,07 dan 5,37 ± 0,05 µm. Hasil Penelitian menunjukkan bahwa kombinasi pH media dan lama inkubasi tidak berpengaruh nyata terhadap morfometri X-Y pada lapisan atas dan bawah (Fhit ≤ F0,05). Disimpulkan bahwa perlakuan memberikan pengaruh yang sama.
Pelatihan Penerapan Sinkronisasi Estrus dan Inseminasi Buatan untuk Peningkatan Produktivitas Budidaya Domba di Desa Pamulihan Kecamatan Pamulihan Kabupaten Sumedang Solihati, Nurcholidah; Rasad, Siti Darodjah; Setiawan, Rangga; Widyastuti, Rini; Sari, Aprilianna P.Z.N.L.; Toha, Toha; Winangun, Kikin
Farmers : Journal of Community Services Vol 7, No 1 (2026): Farmers: Journal of Community Services
Publisher : Unpad Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/fjcs.v7i1.69538

Abstract

Sinkronisasi estrus dan inseminasi buatan (IB) merupakan bioteknologi reproduksi yang dapat membantu manajemen reproduksi di suatu kawasan peternakan dimana peternak dapat mengatur waktu perkawinan yang dapat disesuaikan dengan permintaan pasar. Desa pamulihan Kecamatan Pamulihan Kabupaten Sumedang, memiliki beberapa peternak domba Garut yang memiliki usaha secara komersil dengan jangkauan pemasaran yang cukup luas. Tujuan kegiatan ini yaitu memberikan pengetahuan kepada peternak domba tentang sinkronisasi estrus dan IB untuk dapat diaplikasikan pada sistem pemeliharaan domba yang mereka miliki. Metode yang dilakukan meliputi penyuluhan, diskusi dan tanya jawab, pelatihan dan demonstrasi. Hasil kegiatan menunjukkan adanya peningkatan pengetahuan peternak terhadap materi pelatihan terkait sinkronisasi estrus dan IB yang diketahui dari hasil pre-test dan post-tes, yaitu sebesar 53%. Hasil ini menunjukkan bahwa metode yang dilakukan berupa penyuluhan dan pelatihan cukup efektif memberikan pengetahuan kepada peternak domba di Desa Pamulihan. Disimpulkan bahwa peserta pelatihan mampu menerima ilmu dan keterampilan yang telah diberikan, peserta juga tertarik untuk dapat mengaplikasikan hasil pelatihan terhadap domba yang mereka miliki, namun untuk pelaksanaan secara mandiri masih memerlukan pembinaan dan pendampingan
Vaginal Cytological Changes and Estrus Response Following Estrus Synchronization Using a Double Prostaglandin-F₂α and Gonadotropin Releasing Hormone in Pasundan Heifers Rini Widyastuti; Azis, Azhar Abdul; Setiawan, Rangga; Hilmia, Nena; Lubis, Alkaustariyah; Ridlo, Muhammad Rosyid
Jurnal Veteriner Vol. 27 No. 1 (2026)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/

Abstract

Pasundan cattle, an indigenous breed native to West Java, Indonesia, demonstrate significant adaptability and reproductive potential. However, their declining populations pose a threat to genetic conservation. Effective estrus synchronization is essential for enhancing reproductive efficiency and supporting fixed-time artificial insemination (FTAI) programs. Vaginal cytology profiling offers a reliable, noninvasive method for assessing estrus by evaluating the dynamics of epithelial and leukocyte cells, which reflect hormonal fluctuations. This study was aimed to investigate the cytological dynamics of vaginal epithelial, leukocyte cells and estrus response in Pasundan heifers subjected to double injection of prostaglandin F₂α (PGF₂α) and gonadotropin-releasing hormone (GnRH) synchronization. Nine Pasundan heifers, aged 1.5–2.0 years, were treated with PGF₂α with 2.75 μg/kg BW on days 0 and 11, followed by 2 μg/kg BW GnRH on day 12. Vaginal smears were collected daily from days 0 to 15, stained with 5% Giemsa, and examined microscopically to determine the proportions of parabasal, intermediate, superficial, keratinized, and leukocyte cells. The results indicated that the initial cytology showed a luteal phase dominated by parabasal (31.2%) and intermediate (30.6%) cells. Following hormonal treatment, superficial cells peaked at 70.9% on day 13, indicating estrus and estrogen dominance. By days 14–15, keratinized and parabasal cells had increased, confirming post-ovulatory luteal activity. The protocol achieved complete (100%) estrus synchronization among all heifers. It can be concluded that the double PGF₂ α-GnRH protocol effectively synchronized estrus in Pasundan heifers. Vaginal cytology has proven to be a sensitive and practical tool for monitoring reproductive phases, thereby supporting breeding management and conservation of this native genetic resource.
Identification of Changes in Body Temperature, Vulvar Temperature, Vulvar Length and Width Prior to Parturition in Sapera Goats Afifah, Ummi; Setiawan, Rangga; Solihati, Nurcholidah
Jurnal Produksi Ternak Terapan Vol 7, No 1 (2026): Volume 7 Nomor 1 Tahun 2026
Publisher : Faculty of Animal Husbandry, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/jptt.v7i1.71109

Abstract

Preparation for parturition in goats requires monitoring physiological indicators to improve the accuracy of predicting the timing of parturition and minimize the risk of birthing complications. This study aimed to identify changes in body temperature, vulvar temperature, vulvar length, and vulvar width prior to parturition in Sapera goats. This study utilized eight pregnant Sapera does observed for five days before kidding (D-5 to D0). Parameters analyzed included body temperature, vulvar temperature, vulvar length, and vulvar width, which were then evaluated using Analysis of Variance (ANOVA) and Tukey's HSD post-hoc test. The results showed that body temperature differed significantly (P<0.05), with a significant decrease occurring one day before parturition (D-1). Meanwhile, vulvar temperature, vulvar length, and vulvar width showed a shifting trend but were not significantly different. It is concluded that the decrease in body temperature one day prior to parturition can be used as a predictive indicator for the timing of birth in Sapera goats.