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Radical Scavenging Activity and Quercetin Content of Muntingia calabura L. Leaves Extracted by Various Ethanol Concentration Ratih Dyah Pertiwi; Suwaldi; Ronny Martien; Erna Prawita Setyowati
Journal of Food and Pharmaceutical Sciences Vol 8, No 1 (2020): J. Food Pharm. Sci
Publisher : Institute for Halal Industry and System (IHIS) Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfps.581

Muntingia calabura is broadly cultivated and has become a common roadside tree in Indonesia that is known as "Kersen," or cherry. The current study aimed to determine the activities of the phytochemical constituent (i.e., phenolic and flavonoid total contents) with various concentrations of ethanolic extract of M. calabura leaves (EEMC) to determine the best one and the in vitro antioxidant activities and quercetin content using TLC Densitometry. The extraction was carried out by maceration with various concentrations of ethanol (96% v/v; 70% v/v and 50% v/v) to obtain the ethanolic extract of Muntingia calabura, L leaves. The determination of Quercetin was conducted by Thin Layer Chromatography (TLC) densitometry, and the total phenolic was analyzed with reagent Folin-Ciocalteu while the flavonoid content we identified by applying the colorimetric method. The antioxidant capacity was measured using the radical scavenging assay of 1,2-diphenyl-2-picrylhydrazyl (DPPH). The phytochemical study showed that phenolics, saponins, tannins, and flavonoids were present in all EEMC. The extract obtained by 96% ethanol showed the highest total antioxidant activity, reducing power and DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging activity. The same extract also exhibited the highest flavonoid content. However, the extract obtained by 70% ethanol showed the phenolic content, and the highest quercetin content was obtained by using 50% ethanol. The result from the EEMC analysis indicated that Muntingia calabura leaf extract contains essential bioactive compounds and shows potential as a source of antioxidants in M. calabura which uses 96% ethanol solvent and has the highest quercetin content in M calabura using 50% ethanol solvent.

Korelasi Berat Badan dan Umur Sapi terhadap Berat, Volume Cairan dan Konsentrasi Prostaglandin F2α pada Vesikula Seminalis Naela Wanda Yusria Dalimunthe; Agung Budiyanto; Erna Prawita Setyowati; Agustina Dwi Wijayanti
Jurnal Sain Veteriner Vol 35, No 1 (2017): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Seminal vesicles were collected from 60 heads of Bulls which butchered in slaughter house (RPH) Yogyakarta. The aims of this study are knowing the relationship between body weight, age, fluids volume and concentration of prostaglandin F2 α (PGF2α) in seminal vesicle of beef cattle. Those seminal vesicles were gathered from bulls which recorded its body weight and age then measured its seminal vesicles for weight, fluids volume and PGF2α levels. The PGF2α level was measured by Enzyme-Linked Immunosorbent Assay. Statistical analysis was performed using one way – analysis of varian, regression and correlation with P<0.05. Body weight of bulls showed positive correlation with the weight of seminal vesicle and its fluids volume. However, PGF2α levels were not correlated with the body weight of cattle. Weight of seminal vesicles also exhibited positive correlation with volume of vesicle fluids but no correlation with PGF2α levels. Based on the age of bulls, there were no correlation withthe weight of seminal vesicles, seminal fluids volume and PGF2α levels. Those result indicated that the weight and fluids volume were affected by the body weight of bulls altough the PGF2α levels have a standard of developmentwhich seems affected by other factors such as concentration of androgen hormone.

Optimasi formula gel ekstrak kubis ungu (Brassica Oleracea L. Var. Capitata F. Rubra) menggunakan simplex lattice design dan pengujian aktivitas antioksidan secara in vitro Rima Yulia Senja; Akhmad Kharis Nugroho; Erna Prawita Setyowati
Pharmaciana Vol 6, No 2 (2016): Pharmaciana
Publisher : Universitas Ahmad Dahlan

ABSTRACTA study of optimization of red cabbage (Brassica oleracea.L. var. capitata f. rubra) gel extract formula has been performed by using Simplex Lattice Design (SLD) method and antioxidant activity of the formula gel was also evaluated by using in vitro method.The red cabbage was extracted by soxhletation by using ethanol 96% followed by optimization of red cabbage extract in antioxidant gel preparation used SLD method by Design-ExpertÂ® software version 7 (DX7) and determination of its IC50 used UV-Spectrophotometry. The stability of optimum gel formula is seen through comparison of physycal properties at the beginning and after four weeks storage used ANOVA, with a 95% significant level. Optimum gel formula of red cabbage extract obtained in the proportion of Metolose 3,883%, propilen glikol 13.5%, Tween 80 1.117%. The evaluation results of optimum gel formula of red cabbage extract is the surface area of gel dispersive of 38.99 Â± 3.27cm2; viscosity gel dPa.s of 295.56 Â± 1.93 and viscosity change 3.89 Â± 0.96%. From the results of statistical analysis of one t-test sample was concluded that there was no difference between the prediction price of software with the observation result (p> 0.05). The IC50 test result of the optimum formula of red cabbage extract gel was 257.25 Â± 0.35 Âµg / mL. The testing result of the physical stability of the optimum formula of red cabbage extract gel suffered a pH decrease after 4 weeks of storage (p <0.05).

Radical Scavenging Activity and Quercetin Content of Muntingia calabura L. Leaves Extracted by Various Ethanol Concentration Ratih Dyah Pertiwi; Suwaldi; Ronny Martien; Erna Prawita Setyowati
Journal of Food and Pharmaceutical Sciences Vol 8, No 1 (2020): J. Food Pharm. Sci
Publisher : Institute for Halal Industry and System (IHIS) Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfps.581

Muntingia calabura is broadly cultivated and has become a common roadside tree in Indonesia that is known as "Kersen," or cherry. The current study aimed to determine the activities of the phytochemical constituent (i.e., phenolic and flavonoid total contents) with various concentrations of ethanolic extract of M. calabura leaves (EEMC) to determine the best one and the in vitro antioxidant activities and quercetin content using TLC Densitometry. The extraction was carried out by maceration with various concentrations of ethanol (96% v/v; 70% v/v and 50% v/v) to obtain the ethanolic extract of Muntingia calabura, L leaves. The determination of Quercetin was conducted by Thin Layer Chromatography (TLC) densitometry, and the total phenolic was analyzed with reagent Folin-Ciocalteu while the flavonoid content we identified by applying the colorimetric method. The antioxidant capacity was measured using the radical scavenging assay of 1,2-diphenyl-2-picrylhydrazyl (DPPH). The phytochemical study showed that phenolics, saponins, tannins, and flavonoids were present in all EEMC. The extract obtained by 96% ethanol showed the highest total antioxidant activity, reducing power and DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging activity. The same extract also exhibited the highest flavonoid content. However, the extract obtained by 70% ethanol showed the phenolic content, and the highest quercetin content was obtained by using 50% ethanol. The result from the EEMC analysis indicated that Muntingia calabura leaf extract contains essential bioactive compounds and shows potential as a source of antioxidants in M. calabura which uses 96% ethanol solvent and has the highest quercetin content in M calabura using 50% ethanol solvent.

Health cadre empowerment to use herbal plants for therapy and health in Girirejo Village, Imogiri, Bantul Hastuti, Janatin; Rahmawati, Neni Trilusiana; Setyowati, Erna Prawita
Journal of Community Empowerment for Health Vol 8, No 3 (2025)
Publisher : Faculty of Medicine, Public Health, and Nursing, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jcoemph.100536

Introduction: The high rate of obesity and hypertension in the productive age group demands special attention from various parties for early detection and treatment, which can be accomplished by empowering village health cadres. This community service program is intended to increase knowledge and skills in the use of herbal plants for therapy and health among health cadres in Girirejo Village, Imogiri, Bantul.Methods: This study, done as part of a community service program in September 2023, included 42 health cadres (women aged 25-62 years) from Girirejo Village, Imogiri, Bantul, Yogyakarta. Participants learned about the therapeutic and physiological benefits of using herbal plants. The materials delivered included identifying various herbal materials, making herbal combinations, and employing therapeutic plants for health purposes. Pre- and post-tests were given before and after education. Training is provided through the practice of making herbal infusions. Statistical analysis uses the paired t-test. Results: The results revealed a significant difference (p<0.001) in the mean pre-test (64.50 ± 15.23) and post-test (80.75 ± 15.21). This suggests that this education can help health cadres learn more about knowledge and the utilization of herbal plants.Conclusion: The study concluded that this community service program might increase understanding and skills of the use of herbal plants for the health of Health Cadres in Girirejo Village. Participants indicated their satisfaction with the program's implementation. It is proposed that a new Community Service Strategic Plan, which includes Girirejo Village Health Cadres, be designed as a structured, innovative, and forward-thinking program plan to strengthen the empowerment of health cadres and the natural potential of local wisdom

ANTIOXIDANT ACTIVITY OF INDONESIAN BROWN MARINE ALGAE (Sargassum turbinarioides) Artemisia, Rahma; Sinurat, Ellya; Setyowati, Erna Prawita; Martien, Ronny; Nugroho, Akhmad Kharis
Jurnal Farmasi Sains dan Praktis Vol 11 No 3 (September-December 2025)
Publisher : Universitas Muhammadiyah Magelang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31603/pharmacy.v11i3.9673

Brown marine algae are provided for an excellent source of bioactive compounds especially fucoidan. Fucoidan is primarily composed of L-fucose residues linked by α-1,3 and α-1,4 glycosidic bonds, with sulfate groups as its main chemical components. One of the biological activities of fucoidan is antioxidant. Antioxidant activities of Indonesian brown marine algae were investigated. The phytochemical screening of the crude extract was conducted to identify various phytochemical constituents, including carbohydrates, flavonoids, phenolic compound, terpenoids, and proteins and amino acid. The antioxidant activity of the brown marine algae Sargassum turbinarioides was evaluated using free radical scavenging through the DPPH assay and a reducing power assay. The antioxidant capacity of the extracted fucoidan was measured against ascorbic acid, revealing that fucoidan from brown marine algae exhibited lower DPPH radical scavenging activity compared to vitamin C. Meanwhile, the reducing power of the extract increased with higher concentrations, demonstrating a dose-dependent effect. These findings suggest that brown marine algae extract has potential for commercialization as a functional food or dietary supplement owing to its significant antioxidative properties.

Tinjauan Literatur: Trichoderma sebagai Sumber Penghasil Senyawa Sitotoksik yang Menjanjikan Nurhaliza, Nurhaliza; Setyowati, Erna Prawita; Jenie, Riris Istighfari
Majalah Farmaseutik Vol 22, No 1 (2026)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/farmaseutik.v22i1.105038

Trichoderma merupakan genus fungi yang memiliki aktivitas farmakologi beragam, seperti antifungi, antibakteri, agen biokontrol, dan antikanker. Meski diketahui memiliki aktivitas antikanker, studi mengenai jenis-jenis metabolit sekunder antikanker dari Trichoderma belum tersedia sehingga studi ini dinilai perlu untuk mengkaji potensi Trichoderma sebagai sumber senyawa antikanker melalui analisis aktivitas sitotoksiknya terhadap sel kanker. Tinjauan literatur ini menggunakan pustaka acuan yang mengacu pada pangkalan data Scopus dan Web of Science. Metodologi pemerolehan data menggunakan metode PRISMA (Preferred Reporting Items for Systematic Reviews and Meta Analyses). Dari 117 publikasi yang diperoleh, sebanyak 96 artikel dieksklusi karena tidak memenuhi kriteria sehingga hanya tersedia sebanyak 21 artikel yang dianalisis. Data kemudian disajikan dalam bentuk tabel yang memuat informasi spesies, nama senyawa, nilai IC50, dan metode uji sitotoksik yang digunakan. Trichoderma diketahui menghasilkan beragam metabolit sekunder yang bersifat sitotoksik. Adapun tiga contoh senyawa yang memiliki aktivitas sitotoksik sangat poten adalah isobisvertinol, 18-deoxycytochalasin H, dan harzianum A dengan nilai IC50 berturut-turut adalah 0.5 µM, 0.42 µM terhadap sel A2780, dan 0.65±0.03 µg/mL terhadap sel HT1080.

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