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All Journal Bioscience Biogenesis: Jurnal Ilmiah Biologi BERITA BIOLOGI Jurnal Bioteknologi & Biosains Indonesia (JBBI) BIOEDUKASI BIOEDUSCIENCE ANNALES BOGORIENSES Jurnal Pengabdian Masyarakat AbdiMas AL-ULUM: JURNAL SAINS DAN TEKNOLOGI Biofarm : Jurnal Ilmiah Pertanian SPIN: Jurnal Kimia & Pendidikan Kimia Jurnal Pengabdian kepada Masyarakat Prosiding Seminar Informasi Kesehatan Nasional Jurnal Multidisiplin Sahombu Annales Bogorienses
Febriana Dwi Wahyuni
Dosen Bioteknologi - Universitas Esa Unggul
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Energy Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Medicine & Pharmacology Nursing Public Health Social Sciences Other

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Journal : BIOEDUKASI

 1 
Bioinformatics Analysis to Construct Cellulose-binding Module Synthetic Gene and Design Primer Febriana Dwi Wahyuni; Asrul Muhamad Fuad
BIOEDUKASI Vol 17 No 1 (2019)
Publisher : PROGRAM STUDI PENDIDIKAN BIOLOGI FAKULTAS KEGURUAN DAN ILMU PENDIDIKAN UNIVERSITAS JEMBER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bioedu.v17i1.13204

Abstract

Cellulose-binding module (CBM) is a protein domain commonly found in various types of cellulase enzymes. The function of this CBM can be used for the binding process and the immobilization of a protein in the cellulose matrix. CBM can be obtained from several organisms, one of them is Trichoderma reesei. To get a gene, it does not have to be isolated from the original organism. Gene sequences can be obtained synthetically through bioinformatics analysis in accordance with the same gene sequences as those at Gene Bank. Bioinformatics analysis can be used to find new gene sequences or existing genes. This study aims to get a cbmsyn synthetic gene quickly and efficiently without reducing protein activity, which can then be ligated with other genes so that it functions as an immobilized enzyme. From the results of bioinformatics analysis, obtained DNA sequences measuring around 498 pb with 166 amino acid protein lengths. The sequence was modified by adding several restriction sites, namely BamHI, AfeI, and ScaI. The DNA sequences obtained were optimized with the Pichia pastoris codon.
SUBSTITUTION OF SYNTHETIC HORMONES WITH ORGANIC MATERIALS ON THE GROWTH OF ORCHID PLANTS (Phalaenopsis amabilis) AS A GROWTH REGULATORY SUBSTANCE IN VITRO Febriana Dwi Wahyuni; Kevin Febrianus Moda; Dimas Ridho Irvansah; Novita Sari
BIOEDUKASI Vol 19 No 2 (2021)
Publisher : PROGRAM STUDI PENDIDIKAN BIOLOGI FAKULTAS KEGURUAN DAN ILMU PENDIDIKAN UNIVERSITAS JEMBER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bioedu.v19i2.27141

Abstract

In Indonesia, the agribusiness sector has begun to expand due to the increase in entrepreneurship of orchid plants. Several genera and species of orchid plants are promising to be traded as ornamental plants, one of which is the moon orchid (Phalaenopsis amabilis). However, the limited number of seeds or plants produced by conventional propagation methods takes a long time to get new tillers. Therefore, tissue culture techniques can be used to grow and reproduce moon orchid plants. In the success of in vitro culture, the balance of growth regulators is an essential factor. On the other hand, synthetic hormones such as 1-naphthalenaecetic acid (NAA) and 6-benzylaminopurine (BAP) are relatively expensive. The study in this research is to substitute the synthetic hormones NAA and BAP with organic ingredients that are cheap and easy to find. Such as Purple Sweet Potatoes and Beans and find out the right concentration of organic matter. This study used a completely randomized design with two factorials (concentration of purple sweet potato and green beans) with three repetitions. PLB (Protocorm Like Bodies) moon orchid (Phalaenopsis amabilis) was treated with: positive control (2 ppm), negative control (without the addition of organic or synthetic hormones), and variations in the concentration of purple sweet potato and green beans organic matter. The result was that C4D0 (500g/L purple sweet potato extract and 0 g/L green bean extract) was an excellent formulation, although there was no significant interaction between purple sweet potato and green bean extracts against moon orchid.
In-Silico Analysis for cryI Gene Amplification from Bacillus thuringiensis Febriana Dwi Wahyuni; Henny Saraswati; Kartika Sari Dewi
BIOEDUKASI Vol 18 No 1 (2020)
Publisher : PROGRAM STUDI PENDIDIKAN BIOLOGI FAKULTAS KEGURUAN DAN ILMU PENDIDIKAN UNIVERSITAS JEMBER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bioedu.v18i1.16430

Abstract

Abstract Bacillus thuringiensis is one type of bacteria that has been used as a microbiological control agent for pests and a vector of plant disease. The presence of Cry proteins inside the B. thuringiensis can be acted as a specific insect repellent that only toxic to certain insects. The CryI protein is toxic to Lepidoptera insects which can attack various types of plants. Polymerase Chain Reaction (PCR) is a common method that can be used to amplify the gene encoding CryI proteins from B. thuringiensis. This research aimed to design a good primer candidate for cryI gene amplification from B. thuringiensis. In silico analysis for designing cryI primer was carried out using some software, such as BLAST for searching cryI gene sequence, Bioedit for sequences alignment, and DINAmelt for analyzing dimer structure of primers. Ten primer candidates were successfully obtained based on the result of the primer3 software. A pair of primer was selected to amplify the cryI gene, with forward primer 5’- CGGTGAATGCCCTGTTTACT -3’ and reverse primer 5’-CGGTCTGGTTGCCTATTGAT -3’. Amplification of the cryI gene by PCR method using selected primer resulting in a PCR product with a length of approximately 200 bp.
Optimization of Real-Time PCR Conditions for COVID-19 Diagnosis with Logix Smart Reagent™ Anisa Febriyanti; Seprianto Seprianto; Titta Novianti; Febriana Dwi Wahyuni; Oktaviani Naulita Turnip; Roselein Putri; Henny Saraswati
BIOEDUKASI Vol 20 No 1 (2022)
Publisher : PROGRAM STUDI PENDIDIKAN BIOLOGI FAKULTAS KEGURUAN DAN ILMU PENDIDIKAN UNIVERSITAS JEMBER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bioedu.v20i1.28356

Abstract

Corona Virus Disease 2019 or COVID-19 is a new type of virus that attacks the respiratory tract and can cause death. Laboratory examinations play an essential role in diagnosing COVID-19 with a set of reagents or kits. Sampiand sampling is carried out with a nasopharyngeal swab or oropharyngeal swab. Positive samples of COVID-19 patients used in this study were converted into RNA at the COVID-19 Referral Clinic in Bekasi, after which volume optimization was carried out with a total volume of 5 µl, 8 µl, and 10 µl with the Logix Smart™ kit. The method in this study uses One-Step Real-time PCR. This method is the best method for carrying out several bear tests because it can reduce the possibility of sample contamination. The procedure is fast and has high sensitivity. The fluorescence detection used in this study was FAM with a specific target of COVID-19 RNA and ROX with a particular DNA target of RNase-P. This research was conducted to obtain optimal volume conditions under the manufacturer's standards in detecting the SARS-CoV-2 virus. The results of this study indicate that a total volume of 5 l is the optimal total volume for detecting the presence of the SARS- CoV-2 virus in samples taken from patients.
Co-Authors Agung Suprianto alfero Putra Iryanto Anabella, Anabella Anisa Febriyanti Aroem Naroeni Asrul Muhamad Fuad Asrul Muhamad Fuad Asrul Muhamad Fuad, Asrul Muhamad Atikah Fairus Siti Badriah Aulia, Cika callista marsya Dedi Supriadi Dimas Ridho Irvansah Enung Sri Mulyaningsih feby feby Hani Fitriani, Hani Henny Saraswati Henny Saraswati Henny Saraswati Iis Nur Asyiah Kartika Sari Dewi Kevin Febrianus Moda Meisya Then Septian Muhamad Amza Sidiq Muhammad Arreza Nelly Eka Khusnul Qotimah nie nie Ningsih, Irnawati Nora, Adri Novianti, Titta Novita Sari Oktaviani Naulita Turnip putri mega utami Rahman, Nurhamidar roaslein putri Roaslein Putri Roselein Putri Sabar Pambudi Saputri, Yulia Saraswati, Henny Saraswati, Henny Seprianto Seprianto Seprianto Seprianto Seprianto, Seprianto Slamet Hariyadi Sri Rianawati Suharsono Suharsono Suharsono Suharsono Syafruddin Ilyas