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All Journal Bioscience Biogenesis: Jurnal Ilmiah Biologi BERITA BIOLOGI Jurnal Bioteknologi & Biosains Indonesia (JBBI) BIOEDUKASI BIOEDUSCIENCE ANNALES BOGORIENSES Jurnal Pengabdian Masyarakat AbdiMas AL-ULUM: JURNAL SAINS DAN TEKNOLOGI Biofarm : Jurnal Ilmiah Pertanian SPIN: Jurnal Kimia & Pendidikan Kimia Jurnal Pengabdian kepada Masyarakat Prosiding Seminar Informasi Kesehatan Nasional Jurnal Multidisiplin Sahombu Annales Bogorienses
Febriana Dwi Wahyuni
Dosen Bioteknologi - Universitas Esa Unggul
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Energy Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Medicine & Pharmacology Nursing Public Health Social Sciences Other

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Journal : Bioscience

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Candida antarctica Lipase B Synthetic Gene: A Bioinformatics Analysis Febriana Dwi Wahyuni; seprianto Seprianto
Bioscience Vol 2, No 2 (2018): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (443.901 KB) | DOI: 10.24036/0201822100216-0-00

Abstract

Enzyme exploration is important to support the development of biotechnology. To facilitate microbial discovery, it can be done synthetically using bioinformatics methods. Candida antarctica lipase B (CALB) is one of enzyme derived from microorganisms and has been applied as a biocatalyst in several industry. The methods used in this study are the analysis of gene structure using NCBI, analysis of protein CALB sequence using Uniprot, analysis of 3D protein structure using Swiss model, analysis primary design using primer3 and analysis of restriction sites using snapgene. The construction of synthetic calB gene obtained based on the results of the CalB gene sequence in geneBank using NCBI with access number Z30645.1. CalB gene wildtype is modified by adding several restriction enzyme (XhoI, XbaI, ClaI, and BglI), 6 Histags, and 2x stops codon and produce 1083 base pairs. CALB protein has 342 amino acids. Based on 3D structure, CALB protein has three molecules in common with one homotrimer ring that will encircle the double helix DNA. Primers used for calB gene amplification are CALB forward 5'-TCCCCAGTATCAGGTCCAAG-3 ' and CALB reverse 5'-GACACCTGAGGCTGAACGAT-3'.
Co-Authors Agung Suprianto alfero Putra Iryanto Anabella, Anabella Anisa Febriyanti Aroem Naroeni Asrul Muhamad Fuad Asrul Muhamad Fuad Asrul Muhamad Fuad, Asrul Muhamad Atikah Fairus Siti Badriah Aulia, Cika callista marsya Dedi Supriadi Dimas Ridho Irvansah Enung Sri Mulyaningsih feby feby Hani Fitriani, Hani Henny Saraswati Henny Saraswati Henny Saraswati Iis Nur Asyiah Kartika Sari Dewi Kevin Febrianus Moda Meisya Then Septian Muhamad Amza Sidiq Muhammad Arreza Nelly Eka Khusnul Qotimah nie nie Ningsih, Irnawati Nora, Adri Novianti, Titta Novita Sari Oktaviani Naulita Turnip putri mega utami Rahman, Nurhamidar roaslein putri Roaslein Putri Roselein Putri Sabar Pambudi Saputri, Yulia Saraswati, Henny Saraswati, Henny Seprianto Seprianto Seprianto Seprianto Seprianto, Seprianto Slamet Hariyadi Sri Rianawati Suharsono Suharsono Suharsono Suharsono Syafruddin Ilyas