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All Journal Global Health Management Journal PREPOTIF : Jurnal Kesehatan Masyarakat Tunas Medika Jurnal Kedokteran & Kesehatan Indonesian Journal of Applied Research (IJAR) JPM: JURNAL PENGABDIAN MASYARAKAT InaBHS (Indonesian Journal of Biomedicine and Health Science)
Weni, Mustika
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Antimicrobial Activity of Ethyl Acetate Fraction of Kersen Leaves Against Staphylococcus aureus and Escherichia coli Weni, Mustika; Marfuati, Sri
Indonesian Journal of Applied Research (IJAR) Vol. 5 No. 3 (2024): Indonesian Journal of Applied Research (IJAR)
Publisher : Universitas Djuanda

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30997/ijar.v5i3.531

Abstract

The underutilized leaves of the kersen plant contain secondary metabolite compounds that have antibacterial potential. The aim of this research was to test the antibacterial activity of the ethyl acetate fraction of kersen leaf against Escherichia coli and Staphylococcus aureus and determine the best concentration. Kersen leaf extraction method using the maceration method using ethanol solvent, fractionation method using ethyl acetate solvent and testing the antibacterial activity of kersen leaf against Staphylococcus aureus and Escherichia coli using the well diffusion method. The normality test used was the Shapiro-Wilk test and used the One-way Anova parametric statistical test. The results of the phytochemical screening of kersen leaf contain compounds phenols, saponins, tannins, alkaloids and triterpenoids. Concentration of 20% of the ethyl acetate fraction of kersen leaf can inhibit Escherichia coli by 8,817 mm in the medium category and Staphylococcus aureus by 7,987 mm in the medium category. The inhibitory power of the ethyl acetate fraction is higher in inhibiting Escherichia coli than Staphylococcus aureus.
Dampak Pelatihan Kesehatan Gigi dan Mulut Pada Siswa Sekolah Dasar Dalam Meningkatkan Status Kebersihan Mulut Siswa Weni, Mustika; Mauladani , Muhammad Firqin; Wahidan, Fikri; Salsabila, Wanda Ayu; Yuniarni, Ervina
JPM: Jurnal Pengabdian Masyarakat Vol. 5 No. 4 (2025): April 2025
Publisher : Forum Kerjasama Pendidikan Tinggi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47065/jpm.v5i4.2350

Abstract

Dental caries is one of the most common oral health problems in society. One of the factors suspected to contribute to the occurrence of dental caries is the habit of brushing teeth. This program aims to increase students' knowledge about the importance of dental and oral care and train them in correct tooth brushing techniques. This activity began with counseling on dental health problems that children often face, such as cavities due to bad habits and high sugar consumption. Furthermore, students were given practical training on how to brush their teeth effectively. After the counseling, data collection was continued with a questionnaire to determine children's tooth brushing habits and the incidence of tooth decay. The results of the training showed that students were very enthusiastic and were able to understand and practice the correct tooth brushing technique. The results of the study: the habit of brushing teeth of elementary school children in the category of irregular brushing of teeth was 15 students (34.1%) of respondents from 44 respondents who were sampled. And the occurrence of tooth decay in children was 28 students (63.6%) of 44 respondents who were sampled. The results of the analysis of the relationship between tooth brushing habits and the occurrence of tooth caries. The analysis using the Chi-square test showed a P-Value = 0.361, indicating no significant relationship between tooth-brushing habits and the occurrence of dental caries. This suggests that other factors, such as dietary patterns, particularly frequent consumption of sugary foods like chocolate or candy, may have a greater influence on dental caries occurrence. Therefore, it is necessary to educate students about the importance of maintaining oral hygiene and reducing sugary food consumption to prevent dental caries.
Dampak Optimalisasi Kesehatan Remaja dengan Edukasi Bijak Dalam Penggunaan Obat Kepada Siswa SMP Marfuati, Sri; Weni, Mustika; Brajawikalpa, Rama Samara; Emallia Fitriani
JPM: Jurnal Pengabdian Masyarakat Vol. 6 No. 1 (2025): July 2025
Publisher : Forum Kerjasama Pendidikan Tinggi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47065/jpm.v6i1.2444

Abstract

Self-medication is increasingly common among the general public, including adolescents. However, a lack of proper understanding regarding drug use, storage, and disposal can lead to medication errors that pose serious health risks. This community service activity aimed to improve the knowledge of students at SMP regarding the proper and responsible use of medications through an educational session titled “Using Medicines Wisely.” The activity was conducted on May 24, 2025, by lecturers from the Faculty of Medicine, Universitas Swadaya Gunung Jati (UGJ), using a face-to-face method involving a pre-test, educational material delivery, interactive discussion, and a post-test. Pre-test results showed that only 22% of participants had good knowledge, which increased to 68% after the educational session with the meaning of value. These findings indicate that the activity effectively enhanced students’ understanding of responsible self-medication practices, particularly in recognizing drug types, usage instructions, proper storage, and safe disposal methods. This program is expected to foster wise medication habits from an early age and contribute to the development of a health-conscious society.
INDONESIA KARAKTERISTIK KLINIS INFEKSI PSEUDOMONAS AERUGINOSA DI RUMAH SAKIT GUNUNG JATI Rachman, Faqih Oktavian; Erwin I, Mohamad; Weni, Mustika
PREPOTIF : JURNAL KESEHATAN MASYARAKAT Vol. 9 No. 2 (2025): AGUSTUS 2025
Publisher : Universitas Pahlawan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/prepotif.v9i2.44116

Abstract

Angka kejadian infeksi di Indonesia nosokomial mencapai 15,74%, jauh lebih tinggi dibandingkan negara-negara maju yang berkisar antara 4,8–15,5%. Tingkat mortalitas akibat infeksi Pseudomonas aeruginosa dilaporkan sebesar 15,1%. Penelitian ini bertujuan untuk mengetahui karakteristik klinis pasien dengan infeksi Pseudomonas aeruginosa. Jenis penelitian ini adalah observasional deskriptif dengan teknik total sampling, sehingga diperoleh sebanyak 153 sampel. Dari 153 pasien yang terinfeksi Pseudomonas aeruginosa, mayoritas berjenis kelamin laki-laki (56,9%) dengan kelompok usia balita (0–5 tahun) sebagai kelompok usia terbanyak (34,0%). Tindakan medis yang paling sering dilakukan adalah operasi pada saluran cerna dan abdomen (4,6%). Penggunaan alat invasif, terutama infus intravena, ditemukan pada 136 pasien (35,7%). Diagnosis klinis primer yang paling sering ditemukan adalah gagal napas (17,0%), sedangkan diagnosis sekunder terbanyak adalah bronkopneumonia (6,5%). Sebagian besar pasien (89,7%) tidak memiliki penyakit penyerta. Angka kematian akibat infeksi Pseudomonas aeruginosa dalam penelitian ini mencapai 58,2%, dengan rerata lama perawatan selama 5 hari. Di RSUD Gunung Jati, dari 153 kasus infeksi yang tercatat, kelompok usia balita dan lansia merupakan kelompok paling rentan, dengan dominasi pasien laki-laki (56,9%). Infeksi ini berkaitan erat dengan penyakit infeksi saluran pernapasan akut (ISPA), infeksi saluran kemih (ISK), infeksi luka, serta penggunaan alat medis invasif seperti infus intravena dan ventilator. Sebagian besar pasien juga menjalani tindakan pembedahan, dan beberapa di antaranya memiliki komorbiditas seperti diabetes mellitus, penyakit jantung, dan penyakit paru kronik.
Overview of COVID-19 Test Results (2021–2022) at the Laboratory of the Faculty of Medicine, Universitas Swadaya Gunung Jati, Cirebon, Indonesia Febrianti, Fitri Ayu; Khasanah, Uswatun; Weni, Mustika
GHMJ (Global Health Management Journal) Vol. 7 No. 3s (2024)
Publisher : Yayasan Aliansi Cendekiawan Indonesia Thailand (Indonesian Scholars' Alliance)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35898/ghmj-741097

Abstract

Background: COVID-19 (Coronavirus Disease-19) is a respiratory infection caused by the SARS-CoV-2 virus. In 2020, the World Health Organization (WHO) declared COVID-19 a global pandemic due to the rapid rise in cases and mortality rates worldwide. The pandemic has impacted millions of people and continues to pose a significant global public health threat. Accurate diagnosis of the disease depends on laboratory testing, with RT-PCR widely recognized as the gold standard for confirming COVID-19. Aims: To describe an overview of COVID-19 test results at Laboratory Faculty of Medicine, Universitas Swadaya Gunung Jati, Cirebon, Indonesia, from 2021-2022. Methods: This study used a descriptive observational method. The sample was collected in July 2024 using a total sampling technique. The sample includes all 135,713 patients who had COVID-19 tests at Laboratory Faculty of Medicine Swadaya Gunung Jati Univesity between 2021 and 2022. Data were collected from medical records of COVID-19 test. The variables of this study include gender, age, and COVID-19 test results. A statistical analysis was used to determine frequency distribution. Results: The results of the study showed that among the sample who tested for COVID-19, the majority are male (50.9%) and early adolescent age group (23.9%). RT-PCR test findings were positive in 16.3% of patients and negative in 83.7% of patients. Among PCR positive cases, the majority are female (17.4%), with seniors age groups >65 years accounting for 39.0%. Conclusion: Most of the tested samples are males and early adolescent age groups, with more negative PCR results than positive ones. Among positive cases, the highest number was found in females and seniors age group. It is recommended that females and seniors age groups keep protected during COVID-19 outbreaks. Received: 25 September 2024  |  Reviewed: 22 October 2024  |  Revised: 30 November 2024  |  Accepted: 11 December 2024.
Improving Pulmonary Tuberculosis Treatment Adherence: The role of patient knowledge in Cirebon, West Java, Indonesia Marfuati, Sri; Fitriani, Hikmah; Weni, Mustika; Riawan, Firanda Putri
GHMJ (Global Health Management Journal) Vol. 7 No. 2 (2024)
Publisher : Yayasan Aliansi Cendekiawan Indonesia Thailand (Indonesian Scholars' Alliance)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35898/ghmj-72987

Abstract

Background: With 10 million cases around the world, pulmonary tuberculosis (TB) has been classified as a highly contagious disease and mostly affecting low and middle countries. Having the second highest incident cases in West Java of Indonesia, Cirebon becomes a challenging city in order to reduce the number of TB cases in the country. Aims: This study aims to identify the patients’ knowledge and treatment phases, and how the two factors encourage patients to comply with their medication. Methods :  This cross-sectional observational study was conducted among 91 new pulmonary tuberculosis patients at the Cirebon City Community Lung Health Centre, selected using random sampling. Not only respondent characteristics, but also data on the patients' knowledge levels, treatment phases, and medication adherence were collected using a questionnaire and medical records. To assess the relationship between these variables, the collected data was then analyzed using the Spearman Correlation test. Ethical clearance was obtained from the Health Research Ethics Commission, and informed consent was gathered from all participants. Results: This study reveals the most updated characteristics of the Tuberculosis patients at the Cirebon City Community Lung Health Center aged 15-64 years old with treatment duration ranged 1-6 months. The majority have insufficient knowledge about tuberculosis (45.1%), and 75.8% of patients adhered to their prescribed medication regimen, regardless of their knowledge level. The data indicates a significant positive correlation between knowledge level and medication adherence (p = 0.015), with 95% of patients with good knowledge adhering to treatment compared to only 34% with poor knowledge. Furthermore, there is a significant relationship between adherence and treatment duration (p = 0.002), as 85% of patients who adhered to treatment did so for more than two months. Conclusion: The study shows that patients with better knowledge of tuberculosis are more likely to stick to their medication, which also leads to longer treatment durations. Given the high incidence of TB in the region, these findings suggest the need for targeted educational programs to enhance patients' understanding of TB, thereby improving adherence to treatment protocols. Received: 20 May 2024, Reviewed: 09 June 2024, Revised: 26 August 2024, Accepted: 30 August 2024.
Uji Aktivitas Antibakteri Ekstrak Etanol Daun Kersen (Muntingia calabura L.) terhadap Bakteri Escherichia coli Weni, Mustika; Marfuati, Sri; Fauzah, Shofa Nur; Affandi, Thysa Thysmelia
Tunas Medika Jurnal Kedokteran & Kesehatan Vol 10 No 3 (2024): TUNAS MEDIKA JURNAL KEDOKTERAN & KESEHATAN
Publisher : Fakultas Kedokteran UGJ Cirebon

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33603/tumed.v10i3.9618

Abstract

Permasalahan penyakit infeksi dan penyakit tropik masih menjadi permasalahan yang sangat penting karena angkakesakitan dan kemartian yang cukup tinggi. Escherichia coli adalah salah satu bakteri yang dapat menyebabkaninfeksi. Resistensi Escherichia coli sering dilaporkan terjadi, salah satunya Antimicrobial Resistant in Indonesiatelah melaporkan Escherichia coli resisten terhadap beberapa jenis antibiotik, oleh sebab itu dibutuhkan alternatiflain untuk menghambat pertumbuhan bakteri Escherichia coli. Daun kersen mengandung senyawa flavonoid,tannin, saponin yang diketahui berkhasiat sebagai antibakteri. Tujuan: Mengetahui aktivitas antibakteri ekstraketanol daun kersen (Muntingia calabura L.) terhadap bakteri Escherichia coli. Metode: Penelitian ini merupakanpenelitian eksperimental dengan rancangan penelitian post-test only control grup design. Penelitian inimenggunakan 7 kelompok, 2 kelompok kontrol dan 5 kelompok perlakuan. Kelompok perlakuan terdiri dariekstrak etanol dari daun kersen konsentrasi 2%, 10%, 20%, 40%, 60%. Kelompok kontrol yaitu kontrol positif(K(+)) dengan cotrimoxazole dan kontrol negatif ((K(-)) yaitu Dimethyl Sulfoxide (DMSO) 10%. Data diujimenggunakan uji One-way Anova. Hasil: Rata-rata zona hambat terbesar pada konsentrasi 60% yaitu 10,5 mm,sedangkan rata-rata zona hambat terkecil pada konsentrasi 10% yaitu 6 mm, konsentrasi 2% tidak didapatkanadanya zona hambat Kesimpulan: Terdapat perbedaan yang bermakna pada setiap konsentrasi ekstrak etanol daunkersen terhadap bakteri Escherichia coli dan Kadar hambat minimum dari ekstrak etanol daun kersen terhadapbakteri Escherichia coli ada pada konsentrasi 10% dengan rata-rata zona hambat 6 mmKata Kunci: Ekstrak Daun Kersen, Escherichia coli, Aktivitas AntibakteriABSTRACTThe problem of infectious diseases and tropical diseases is still a very important problem because the morbidityand mortality rates are quite high. Escherichia coli is a bacteria that can cause infection. Escherichia coliresistance is often reported, one of which is Antimicrobial Resistance in Indonesia which has reported thatEscherichia coli is resistant to several types of antibiotics, therefore other alternatives are needed to inhibit thegrowth of Escherichia coli bacteria. Cherry leaves contain flavonoid compounds, tannins, saponins which areknown to have antibacterial properties. Objective: To determine the antibacterial activity of ethanol extract ofcherry leaves (Muntingia calabura L.) against Escherichia coli bacteria. Methods: This research is anexperimental study with a post-test only control group design. This study used 7 groups, 2 control groups and 5treatment groups. The treatment group consisted of ethanol extract from cherry leaves at concentrations of 2%,10%, 20%, 40%, 60%. The control group was the positive control (K(+)) with cotrimoxazole and the negativecontrol (K(-)) namely Dimethyl Sulfoxide (DMSO) 10%. Data were tested using the One-way Anova. Results:Obtained the largest average inhibition zone at a concentration of 60%, namely 10,5 mm, while the smallestaverage inhibition zone at a concentration of 10%, namely 6 mm, concentrations of 2% found no inhibition zoneConclusion: There is a significant difference in each concentration of the ethanol extract of cherry leaves againstEscherichia coli bacteria and the minimum inhibition level of the ethanol extract of cherry leaves againstEscherichia coli bacteria is at a concentration of 10 % with an average inhibition zone of 6 mm
Antibacterial Activity of Ethyl Acetat Fraction of Basil Leave (Ocimum Basilicum L) Toward Escherichia Coli Growth Marfuati, Sri; Weni, Mustika; Putri, Inayati Diana
Indonesian Journal of Applied Research (IJAR) Vol. 5 No. 1 (2024): Indonesian Journal of Applied Research (IJAR)
Publisher : Universitas Djuanda

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30997/ijar.v5i1.433

Abstract

Antibiotic resistance to Escherichia coli is commonly found. Several studies have found that basil leaves contain chemical compounds: flavonoids, alkaloids, tannins, and saponins, which act as antibacterials. This research aimed to determine the inhibitory power of the ethyl acetate fraction of basil leaves (Ocimum basilicum L) on the growth of Escherichia coli. This study used a post-test-only control group design. This study contained five groups: one positive control group, one negative control group, and three treatment groups. The treatment group consisted of the ethyl acetate fraction of basil leaves at 12.5% μ, 25% μ, and 50% μ. The control group was a positive control K(+) with co-trimoxazole and a negative control K(-) with 10% Dimethyl Sulfoxide (DMSO). Kruskal walls test was chosen, followed by the Mann-Whitney difference test. In the Kruskal Walls test, a significant difference was found (p-value < 0.050) for basil leaf ethyl acetate fraction treatment. The largest average was obtained, namely the ethyl acetate fraction with a concentration of 12.5%. The Mann-Whitney test was carried out to determine fundamental differences between groups. The results of ethyl acetate fraction showed that the Minimum inhibitory concentration (MIC) for Escherichia coli was 12.5%.
UJI AKTIVITAS ANTIBAKTERI FRAKSI ETIL ASETAT DAUN KEMANGI (Ocimum basilicum L) TERHADAP PERTUMBUHAN BAKTERI Escherichia coli Putri, Inayati Diana; Marfuati, Sri; Weni, Mustika
InaBHS (Indonesian Journal of Biomedicine and Health Science) Vol 3 No 2 (2024): Indonesian Journal of Biomedicine and Health Science
Publisher : Fakultas Kedokteran UGJ Cirebon

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33603/inabhs.v3i2.10523

Abstract

Latar Belakang : Escherichia coli merupakan bakteri penyebab diare. Banyak yang melaporkan terjadinya resistensi antibiotik terhadap Escherichia coli. Oleh karena itu dibutuhkan alternatif pengobatan. Beberapa penelitian mengatakan Daun Kemangi mengandung zat metabolit sekunder seperti alkaloid, flavonoid, saponin dan tanin yang berfungsi sebagai antibakteri. Tujuan : Mengetahui Daya Hambat Fraksi Etil Asetat Daun Kemangi (Ocimum basilicum L) Terhadap Pertumbuhan Escherichia coli. Metode : Penelitian ini adalah penelitian eksperimental laboratorium dengan rancangan penelitian post-test only control grup design. Penelitian ini menggunakan 5 kelompok, yaitu 2 kelompok kontrol dan 3 kelompok perlakuan. Kelompok perlakuan terdiri dari fraksi etil asetat dari daun kemangi konsentrasi 12,5%, 25% , 50% . Kelompok kontrol yaitu kontrol positif (K(+)) dengan cotrimoxazole dan kontrol negatif (K(-)) yaitu Dimetil Sulfoksida (DMSO) 10%. Data diuji menggunakan uji Kruskal wallis dan dilanjutkan dengan uji beda Mann Whitney Hasil : Pada uji Kruskal walls terdapat perbedaan yang signifikan (p-value < 0,050), terhadap pemberian perlakuan fraksi etil asetat daun kemangi. Didapat rerata terbesar yaitu pada fraksi etil asetat konsentrasi 12,5% 28960000000. Dilanjutkan dengan uji Mann whitney untuk mengetahui perbedaan antar kelompok, didapatkan perbedaan daya hambat pada masing-masing konsentrasi Kesimpulan : Kadar hambat minimum dari fraksi etil asetat daun kemangi terhadap pertumbuhan Escherichia coli konsentrasi 12,5% dengan nilai TPC sebesar 28960000000, dan terdapat perbedaan yang bermakna setiap konsentrasi fraksi etil asetat daun kemangi terhadap pertumbuhan Escherichia coli Kata Kunci : Daun Kemangi, Fraksi, Escherichia coli
The Antibacterial Activity Test using Ethyl Acetate Fraction from Kersen Leaves (Muntingia calabura L.) against the Staphylococcus aureus Fadilah, Mutiara; Weni, Mustika; Marfuati, Sri
GHMJ (Global Health Management Journal) Vol. 9 No. 1 (2026)
Publisher : Yayasan Aliansi Cendekiawan Indonesia Thailand (Indonesian Scholars' Alliance)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35898/ghmj-911232

Abstract

Background: Staphylococcus aureus is a common cause of skin and soft tissue infections (pyoderma) and has developed resistance to multiple antibiotics. Kersen (Muntingia calabura L.) leaves contain bioactive compounds such as flavonoids, triterpenoids, steroids, tannins, and saponins, which have been reported to possess antibacterial properties. Aim: This study aimed to evaluate the in vitro antibacterial activity of the ethyl acetate fraction of M. calabura leaves against S. aureus. Methods: An experimental study with a post-test-only control group design was conducted. Phytochemical screening was performed to identify the secondary metabolites present in the fraction. Antibacterial testing was done using the well diffusion method on Mueller-Hinton Agar (MHA). Five treatment groups (ethyl acetate fractions at concentrations of 100%, 60%, 20%, 10%, and 1%) were compared to a negative control (10% DMSO) and a positive control (doxycycline). Inhibition zones were measured manually in millimeters and analyzed using one-way ANOVA (p < 0.05). Results: The ethyl acetate fraction demonstrated a concentration-dependent antibacterial effect. Mean ± SD inhibition zones were 17.20 ± 1.92 mm (100%), 12.94 ± 1.13 mm (60%), 7.99 ± 0.70 mm (20%), 7.34 ± 0.35 mm (10%), and 6.71 ± 0.85 mm (1%). The positive control showed a significantly higher inhibition zone (33.82 ± 1.62 mm), while the negative control showed no inhibition. Phytochemical screening of the kersen leaf ethyl acetate fraction includes tannins, saponins, steroids, triterpenoids, and phenolics. Conclusion: The ethyl acetate fraction of M. calabura leaves exhibited antibacterial activity against S. aureus. These findings support its potential development as a plant-based antibacterial agent, although further in vivo studies are needed.
Co-Authors Afandi, Thysa Thysmelia Amelia, Rosa Daniel Happy Putra Emallia Fitriani Erwin I, Mohamad Fadilah, Mutiara Fauzah, Shofa Nur Fitri Ayu Febrianti Hikmah Fitriani Marfuati, Sri Mauladani , Muhammad Firqin Nirmalasari, Endah Putri, Inayati Diana Rachman, Faqih Oktavian Rahadian, Zulfikar Rama Samara Brajawikalpa Ramadhinia, Nadya Riawan, Firanda Putri Salsabila, Wanda Ayu Thysa Thysmelia Affandi Wahidan, Fikri Widiani, Uci Yuniarni, Ervina Zuliani, Rizki