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All Journal Buletin Udayana Mengabdi E-Jurnal Medika Udayana Jurnal Veteriner ISM (Intisari Sains Medis) : Jurnal Kedokteran Neurologico Spinale Medico Chirurgico Bali Anatomy Journal Jurnal Kefarmasian Indonesia Jurnal Sains dan Kesehatan
I Gusti Nyoman Sri Wiryawan
Departemen Histologi, Fakultas Kedokteran, Universitas Udayana, Denpasar, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Education Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Veterinary

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Journal : Jurnal Veteriner

 1 
Minyak Buah Merah Meningkatkan Aktivitas Proliferasi Limfosit Limpa Mencit Setelah Infeksi Listeria Monocytogenes Ika Wahyuniari; Marsetyawan HNE Soesatyo; Muhammad Ghufron; Yustina -; Andwi Ari Sumiwi; Sri Wiryawan
Jurnal Veteriner Vol 10 No 3 (2009)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The success of individual in keeping healthy from infectious disease is closely related to the ability oftheir body immune responses againts infectious agents. It is reported that one way to increase the immuneresponses is by system to antioxidant. Red fruit oil contains carotenoid and tocoferol that function asantioxidant. This study was aimed at investigating the effect of red fruit oil on spleen lymphocyteproliferation in mice infected with Listeria monocytogenes. Sixty female Balb/c mice were used in thisstudy. The animals were randomly selected and divided into five groups, each group were 12 mice. The firstand second groups received aquadest only, whereas the third, fourth, and fifth groups received red fruit oilwith different doses, ie. 0.3, 0.6, and 1.2 mL/kbBW/day, respectively. Lymphocyte proliferation activitieswere tested by using 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay method.Lymphocyte proliferation assay demonstrated that there was significant difference between the groupswith red fruit oil and aquadest, started at day 2 post-infection. The optical density (OD) values recorded atday 3 in groups I, II, III, IV, and V were 0.769 ± 0.025, 0.904 ± 0.048, 1.110 ± 0.020, 1.021 ± 0.033, 0.979 ±0.002, respectively. The highest optical density (OD), ie. 1.194 ± 0.032, occurred at day 6 after receiving 0,3mL/kgBW/day. In conclusion, red fruit oil could increase lymphocyte proliferation.
Ekstrak Biji Klabet Menurunkan Jumlah Sel Spermatozoa pada Kelinci I Gusti Nyoman Sri Wiryawan; Ida Ayu Ika Wahyuniari
Jurnal Veteriner Vol 10 No 2 (2009)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Fenugreek seed (Trigonella foenum-graecum) contains saponin diosgenin, wich has an antifertility effect on spermatozoa so it can be used as an oral contraceptive drug. This study was aimedto investigate the effect of fenugreek seed extract to spermatogenic process of rabbit, especially onviability spermatozoa. “Completely randomized control group post-test only design” was used inthis study. The animals were divided into four groups; one control group and three treatmentgroups with six replicates (P0 = control group; P1 = group were given 10 % fenugreek seed extract,1 cc/day; P2 = group were given 20 % fenugreek seed extract, 1 cc/day; P3 = group were given 30 %fenugreek seed extract, 1 cc/day). The extract was given orally once a day in 50 days. After treatment,testicles were sectioned and stained with Hematoxylin Eosin; for qualitative and quantitativemicroscopic analysis. The result of this study showed that the number of spermatozoa were decreasedsignificantly (p<0,05) after receiving 10% fenugreek seed extract 1 cc per day. In conclusion,fenugreek seed extract could reduce the number of spermatozoa.
Growth Hormone Menurunkan Ekspresi Protein p53 dan p21 Sel Endotel Tikus Jantan (GROWTH HORMONE REDUCES P53 AND P21 ENDOTHELIAL PROTEIN EXPRESSION IN MALE RATS) I Gusti Ayu Dewi Ratnayanti; Ni Putu Sriwidyani; I Dewa Ayu Inten Primayanti; I Gusti Kamasan; Nyoman Arijana; I Gusti Nyoman Sri Wiryawan; Ida Ayu Ika Wahyuniari; I Wayan Sugiritama; I Gusti Ngurah Mayun
Jurnal Veteriner Vol 17 No 3 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The use of growth hormone (GH) treatment in aging related condition such as atherosclerosis is stillcontroversial. Previous study showed GH reduce atherosclerotic plaque and prevent endothelial cellsenescence. This study was aimed to understand the mechanism of GH effect to endothelial senescencethrough p53/p21 pathway. A randomized posttest only control group design study was conducted. Twentymale Wistar rats were randomized into five groups; negative control (P0), positive control (P1), and GHtreated group (P2, P3, P4). Negative control group was fed with standard diet, and others were fed withatherogenic diet for 20 weeks. After 10 weeks, subjects were injected subcutaneously (0,1 mL) with aquadest(P0 and P1) and increasing dose of GH (0,02 IU, 0,04 IU, and 0,08 IU) for P2, P3, P4 once a day respectivelyfor 10 weeks. In the end of the study all subjects were examined for p53 and p21 endothelial proteinexpressions. Immunohistochemistry of endothelial p53 showed reduce expression in treated groups (P0:7.28 ± 0.36; P1: 39.51 ± 1.18; P2: 32.70 ± 1.10; P3: 16.98 ± 0.78; and P4: 14.29 ± 0.38). The reduction was also observed in p21 expression (P0: 5.38 ± 0.49; P1: 37.81 ± 0.76; P2: 26.02 ± 1.54; P3: 16.37 ± 1.24; andP4: 4.82 ± 0.61. One way analysis of variance and post hoc test (LSD) analysis showed significant differencesbetween all groups (p<0.05). In conclusion, GH reduces endothelial expression of p53 and p21 and thispathway may contribute to GH effect on atherosclerotic plaque and endothelial senescence.
Perbedaan Gambaran Histopatologi Granuloma Paru Mencit Setelah Diinfeksi Mycobacterium tuberculosis dan atau Intervensi Silika (THE INFLUENCES OF TIME IN THE HISTOPATHOLOGY OF LUNG GRANULOMA IN MICE AFTER INFECTION OF MYCOBACTERIUM TUBERCULOSIS AND SILI Ni Made Linawati; I Gusti Ngurah Mayun; I Gusti Nyoman Sri Wiryawan; Nyoman Sri Budayanti; Ni Made Mertaniasih; Fedik Abdul Ratam; I Nyoman Wande; I Gusti Ayu Dewi Ratnayanti; Ida Ayu Ika Wahyuniari; I Wayan Sugiritama; I Gusti Kamasan Arijana
Jurnal Veteriner Vol 14 No 1 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The characteristics of lung tuberculosis is granuloma, which is consisted of lymphocytes andmacrophages that show the interaction between immune cells and M.tb. Granuloma is the organizationprocess which is depend on lymphocytes invasion, adhesion molecules and chemokine fasilitation. Silicosiswhich is caused by silica, can influence granuloma in the lung. The features of granuloma is variationdepend on the elicited agent and immune reaction. The main purpose of this study was to prove thehistopathology differences of  mice lung granuloma caused by M.tb infection,  silica intervention and bothin 3th  and 7th weeks. It was 45 mice Balb-c strain, divided into 3 groups;  P1 got  M.tb infection with H37Rvstrain 105  perml,P2 got silica intervention with 60 micro litre and, P3 got both of M.tb infection and  silica intervention. Termination of each group were held on 3 and 7 weeks of intervention, continued byhistopathology examination. In the histopathology feature, we done semi-quantitative prosedure to measurelung damage by using Dormans scores; perivasculitis, peribronchiolitis, alveolitis and granuloma. Oneway anova to analysis the differences of histopathologycal result among these groups (P< 0,05).  Resultshowed the significant differences  among these group.  In the 3th weeks, we found  mild lung damage werehappened in all groups with granuloma, without necrosic (P1 and P2). In the 7th weeks we found  severe lungdamage in P3 with necrotic and fibrotic granuloma sign, with necrosis in P1, with fibrotic in P2.  Weconcluded the worst lung damage happened in 7th weeks in group which are got M.tb infection and silicaintervention, with granuloma characterictic of necrosic and fibrotic.
Penurunan Kadar Trigliserida pada Tikus Jantan dengan Terapi Growth Hormone (DECREASE OF TRIGLYCERIDE LEVEL IN MALE RAT BY GROWTH HORMONE TREATMENT) I Gusti Ayu Dewi Ratnayanti; I Wayan Sugiritama; Ida Ayu Ika Wahyuniari; Ni Made Linawati; I Gusti Ngurah Mayun; Dewa Ayu Agus Sri Laksemi; I Gusti Ngurah Sri Wiryawan; I Gusti Kamasan Nyoman Arijana
Jurnal Veteriner Vol 14 No 2 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The use of growth hormone (GH) as cardiovascular disease treatment is still controversial. In thispreliminary study the effect of growth hormone therapy on plasma triglyceride level in dyslipidemia wasexamined. Pre and post control group design study was done using 20 dyslipidemic (total cholesterol >200mg/dL) male rats, age 11–12 month-old. The subjects were divided into four groups, aquadest (P0), GH0.02 IU/day (P1), GH 0.04 IU/day (P2), and GH 0.08 IU/day (P3). All subjects were given high cholesteroldiet for three weeks to achieve dyslipidemic in blood level. Aquadest and GH were injected subcutaneouslyonce daily for two weeks. Triglyceride plasma level was measured on day 22nd and 38th by using colorimetricenzymatic test. The mean of pre test plasma triglyceride level of all groups was 136.30 mg/dL and nosignificant difference was found among the groups (p > 0.05). Growth hormone therapy significantly reducedplasma triglyceride level of P1 by 11.78% (118.82 mg/dL, p < 0.01), P2 by 23.46% (103.41 mg/dL, p < 0.01),and P3 by 35.15% (90.22 mg/dL, p < 0.01). Comparison of  post test data amomg the groups showedsignificant difference (p < 0.01). This study show that growth hormone therapy could reduce plasmatriglyceride level in dyslipidemic rat. However, further research is needed to more understand the effect ofthe therapy on cardiovascular diseases.
Co-Authors Andwi Ari Sumiwi Arijana, I Gusti Nyoman Kamasan Dewa Ayu Agus Sri Laksmi Dewi Wijayanti, Ni Putu Ayu Dinesh Tanabbal Dyta Putri Aryo Eswariy Chandramogan Fedik Abdul Ratam GAP Ratih Pradnyandari Gede Reynaldi Wahyu Sanjaya Gede Wirata I Dewa Ayu Inten Primayanti I Gusti Ayu Dewi Ratnayanthi I Gusti Ayu Dewi Ratnayanti I Gusti Kamasan I Gusti Kamasan Arijana I Gusti Kamasan Nyoman Arijana I Gusti Kamasan Nyoman Arijana I Gusti Ngurah Mayun I Made Perdana I Nyoman Wande I Wayan Sugiritama I. G. A. D. Ratnayanthi Ida Ayu Ika Wahyuniari Ida Ayu Ika Wahyuniari Linawati N.M Luh Made Mas Rusyati Marsetyawan HNE Soesatyo Mayun G.N Melita Soares Cristovao Muhammad Ghufron N. M. Linawati Ni Made Linawati Ni Made Linawati Ni Made Mertaniasih Ni Nyoman Sri Budayanti Ni Putu Candra Paramita Ni Putu Sriwidyani Putu Diah Pratiwi Raditya Pradipta Ratnayanthi, I Gusti Ayu Dewi Raymond Elbert Budianto Riezky Januar Pramitha Ruthirar Kalaichelvam Yustina -